Oscar Benito-Román

University of Burgos | UBU · Department of Biotechnology and Food Science

The purpose of this work was to study the purification and isolation (downstream process) of the β-glucan extracted from barley in an ultrasound assisted extraction (UAE) process. The co-extracted starch (average concentration 5.2 ± 0.1 g/L)was hydrolyzed by means of α-amylase. The optimization of the hydrolysis length, temperature and enzyme dose...

Pressurized hot water (PHW) is a new and promising solvent for the extraction of β-glucans from cereals. The effect of temperature, extraction time and pressure has been studied for the extraction of β-glucans from waxy barley (6.0% in β-glucans). Extraction yield, molecular weight and degradation products have been evaluated. Temperature (110–180...

The effect of operational variables on the ultrasound assisted extraction (UAE) of β-glucans from barley has been studied. The selected sonication variables were amplitude, time and cycle, and their combination represents different amounts of energy. The RSM Box–Behnken approach has been used to maximize both extraction yield and molecular weight (...

The purpose of this work is to provide a complete study of the influence of several operational parameters on the aqueous extraction of β-glucan from barley, and to find an optimal combination of factors that maximize the extraction yield. Temperature, pH, extraction time, particle size, stirring rate and solvent:flour ratio were identified as the...

The performance of a microbial fuel cell (MFC) was investigated at different temperatures and anodic media. A lag phase of 30 h occurred at 30 degrees C which was half that at room temperature (22 degrees C). The maximum power density at 30 degrees C was 70 mW/m(2) and at 22 degrees C was 43 mW/m(2). At 15 degrees C, no successful operation was obs...

Onion skin waste (OSW), the primary non-edible byproduct from onion processing, offers a renewable source of bioactive compounds. This study aims to valorize OSW through subcritical water extraction (SWE), aligning with a circular economy and biorefinery principles. SWE was carried out at 145 °C and 50 bar for 50 min in a discontinuous reactor, pro...

Onion skin waste (OSW), the primary non-edible byproduct from onion processing, offers a renewable source of bioactive compounds. This study aims to valorize OSW through subcritical water extraction (SWE), aligning with circular economy and biorefinery principles. SWE was carried out at 145 °C, 50 bar and 50 min in a discontinuous reactor, producin...

The hydrolysis of the water-soluble protein (WSP) fraction from tuna fish meal was evaluated by subcritical water (subW) by using N2 and CO2 as different pressurization agents in the temperature range from 140 to 180 °C. For both gases, the amino group release increased by increasing working temperature while the Lowry response decreased due to pro...

The semi-continuous hydrolysis of onion skin wastes (OSW) using subcritical water (SubW; working conditions: 105-180 ºC; 5 MPa; 2.5 mL/min) has been studied in this work. Liquids after hydrolysis showed a sharp increase in total organic carbon (>30 mg/g OSW) at temperatures above 145 ºC, when SubW began to promote the partial hydrolysis of the stru...

In this work, the optimization of two extraction methods, conventional CE and ultrasound-assisted UAE, to obtain extracts from cornelian cherry fruit with high antioxidant activity, which can be used to produce healthier jelly candies, is presented. In the CE process, the effects of temperature (30–50 °C), time (15–45 min), and hydroalcoholic mixtu...

In this work, the optimization of two extraction methods, conventional CE and ultrasound-assisted UAE, to obtain extracts from cornelian cherry fruit with high antioxidant activity, which can be used to produce healthier jelly candies, is presented. In the CE process, the effects of temperature (30-50 • C), time (15-45 min), and hydroalcoholic mixt...

In this work, the intensification of the extraction of flavonoids from dry onion skin wastes (DOSW) by Ultrasound Assisted Extraction (UAE) was investigated. Conventional stirred tank extraction was used to find the optimal temperature (37 °C) and solvent (ethanol 70%, v/v), providing 20.7 ± 0.4 mg QE/g DOSW after 1 h. In the UAE process, the ampli...

A complete chemical characterization of Moringa oleifera leaves was carried out showing a high content of extractives. Extraction kinetics of bioactive compounds present in this fraction were performed by conventional and ultrasound assisted extraction (UAE). A 50 % (v/v) hydroalcoholic mixture led to the highest total phenolic compounds yield by c...

Ru/MCM48 catalyst was used in the hydrolysis of cellobiose leading to complete hydrolysis and 80% glucose yield at 180 °C and 20 min with loadings of 0.14 g/g of cellobiose. The one-pot hydrolytic hydrogenation of cellobiose was optimum at 180 °C with a catalyst load of 0.57 g/g of cellobiose: after 10 min, a conversion of 86% was achieved with an...

The valorization of the brewery’s spent grain (BSG) generated in a craft beer industry was studied by subcritical water hydrolysis in a semi-continuous fixed-bed reactor. Temperature was varied from 125 to 185 °C at a constant flow rate of 4 mL/min. Biomass hydrolysis yielded a maximum of 78 % of solubilized protein at 185 °C. Free amino acids pres...

The valorization of onion skin wastes (OSW) through the extraction, identification, and quantification of phenolic compounds was studied in this work, using subcritical water in a semicontinuous extractor (2.5 mL/min; 105–180 °C; 5 MPa). The extraction of flavonoids resulted to be fast (<30 min) and temperature sensitive (maximum at 145 °C; total f...

In this work, the polymorphic α-magnesium borohydride form was infiltrated by wet impregnation using tetrahydrofuran (THF) as solvent and subcritical carbon dioxide as innovative drying process. Pressure infiltration at high temperature was also tested as another promising method for confinement. After infiltration, onset decomposition temperature...

The extraction of ferulic acid (FA) and other phenolic compounds from wheat bran can represent an interesting valorization step of this lignocellulosic biomass. In this work, pressurized hot water (PHW) and pressurized aqueous ethanol are used as solvents, and the amount of extracted FA in its free form and the total phenolic content of the extract...

In this work, the encapsulation of rice bran oil extracted using supercritical CO2 has been studied. In the first stage, the emulsification process by high pressure homogenization was studied and optimized. The effect of the working pressure (60–150 MPa), the composition of the carrier (mixtures of pea protein isolate (PPI) and maltodextrin (MD), f...

Brewer’s spent grain (BSG) was chemically characterized obtaining 52.1% of carbohydrates, 17.8% protein, 5.9% lipids, 13.5% insoluble lignin and 24.3% of water-soluble extractives. This work has been focused on the study of polyphenol extraction of the extractive fraction by water ultrasound-assisted extraction. Selected extraction conditions were...

In this work, as a first step in a comprehensive strategy for the valorization of rice bran, the extraction of oil using supercritical CO2 and ethanol as cosolvent has been studied. The effect of extraction temperature (40 and 60 °C), pressure (30 and 40 MPa) and amount of ethanol used (0, 5 and 10%) has been considered. The quality extracted oil h...

Supercritical carbon dioxide (SC−CO2) has been used as solvent in the ethanolysis of fish oil rich with omega-3 polyunsaturated fatty acids (n-3 PUFAs) by Lipozyme 435. The effect of experimental conditions: initial ethanol-to-oil molar ratio (MR, 6:1-76:1), pressure (10–30 MPa), and temperature (323–343 K) on the reaction performance has been stud...

The extraction of polyphenoloxidase (PPO) from Litopenaeus vannamei was studied at different temperatures, pH and ionic strength conditions. Optimum extracts at pH = 7.2 were treated by hiph pressure carbon dioxide (HPCD) technology at 20 MPa and in a temperature range from 35 to 50 °C. Inactivation kinetics for PPO extracts were determined and fit...

Particles from Gas-Saturated Solutions (PGSS)-drying has been used as a green alternative to encapsulate omega-3 polyunsaturated fatty acids (n-3 PUFAs) at mild, non-oxidative conditions. PGSS-dried particles have been compared to those obtained by conventional drying methods such as spray-drying and freeze-drying, finding encapsulation efficiencie...

In the present work, High Pressure Carbon Dioxide (HPCD) has been used to inactivate the enzymes Polyphenol Oxidase (PPO) and Pectin Methylesterase (PME). The work has been done in two stages: in the first one, fundamental knowledge about the inactivation mechanism induced by HPCD was obtained using pure commercial enzymes. The influence of the mai...

In the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatic...

A “green” simple, and low-cost sample extraction procedure involving the use of a deep eutectic solvent (DES) in a vortex assisted liquid-liquid microextraction (VALLME) technique followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis was developed for the simultaneous determination of different PBDEs congeners and OCPs residue...

Tyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance....

The supercritical CO2 extraction of oil from four different quinoa varieties has been studied in this work. For this purpose, the influence of extraction temperature (40–60 °C), pressure (20–40 MPa) and raw material size (250–1000 μm) on the extraction rate has been considered. The extraction rate resulted to be faster the higher the pressure where...

Cloudy apple juice has been treated by thermosonication in batch mode as an alternative processing to thermal treatment. Thermosonication was found to be effective to inactivate polyphenol oxidase; however, pectinmethylesterase was found to be more resistant. An increase of the working ultrasound amplitude and the amount of energy supplied to the j...

Grape marc polyphenols encapsulation in natural origin carriers by high pressure and supercritical fluid techniques

The purpose of this work was to study the behavior of (1-3)(1-4)- β -D-glucan in pressurized hot water. For this purpose, solid β -glucan (450 kDa) was put in water and heated at different temperatures (120, 150, and 170°C) for different times (5 to 360 minutes). At 120°C it was found that the highest soluble β -glucan concentration was measured af...

(1,3)-(1,6)-B-d-Glucans were extracted from Ganoderma lucidum (34.2%, w/w) using pressurized hot water as solvent (P = 5 MPa) in a fixed bed laboratory scale unit. A RSM Box–Behnken experimental design was used to evaluate the effect of the temperature (135–175ªC), flowrate (0.7–1.3 mL/min) and solvent to biomass ratio (20–60 mL/g) on the extractio...

Beta-glucans and phenolics were extracted from waxy barley using pressurized aqueous ethanol in a stirred batch reactor at 25 bar and 500 rpm. The effect of temperature (135–175 °C), extraction time (15–55 min) and ethanol content (5–20%) was evaluated. Temperature had an opposite effect on the extraction of both compounds. The higher the temperatu...

beta-Glucans were successfully extracted from different cereals (barley, de-starched barley and wheat bran) in a fixed-bed extractor using pressurized hot water (PHW) as solvent, in the range of 110-175 degrees C, at 20 bar. Results were evaluated in terms of extraction yield, molecular weight of the extracted (beta-glucans and products of hydrolys...

(1,3)-(1,6)-β-d-Glucans were extracted from Ganoderma lucidum (34.2%, w/w) using pressurized hot water as solvent (P = 5 MPa) in a fixed bed laboratory scale unit. A RSM Box-Behnken experimental design was used to evaluate the effect of the temperature (135-175 °C), flow rate (0.7-1.3 mL/min) and solvent to biomass ratio (20-60 mL/g) on the extract...

β-Glucans were successfully extracted from different cereals (barley, de-starched barley and wheat bran) in a fixed-bed extractor using pressurized hot water (PHW) as solvent, in the range of 110–175 °C, at 20 bar. Results were evaluated in terms of extraction yield, molecular weight of the extracted β-glucans and products of hydrolysis. Extractio...

The effect of the milling on the extraction of beta-glucans has been studied with a hull-less waxy genotype barley cultivar (H13, commercially known as GALIS, with a beta-glucan content of 4.64 +/- 0.16 % and a 58.5 +/- 3.6 % of starch). Barley was milled in a Chopin CD1 mill, obtaining three different milling fractions: brans (50 % w/w; 4.5 % beta...

The aim of this work is to study the effect of different pretreatments on the extraction of β-glucan from barley in order to optimize the overall process. Two different treatments were studied separately: ultrasounds, to enhance the final extraction yield, and ethanol to increase the molecular weight of those β-glucans extracted. Barley flour was b...

β-glucans are non-starchy polysaccharides composed of (1-3), (1-4) mixed linked glucose polymers. They are located mainly in the cell walls of the endosperm and the aleurone layer of different cereal grains, specially oat and barleys. In general, β-glucans are a major component of the soluble dietary fibre and they influence the nutritional values...