Osamah Batiha

Jordan University of Science and Technology | Just · Department of Biotechnology and Genetic Eng

Background and aim: Asthenozoospermia is a condition characterized by reduced sperm motility. Oxidative stress is known to impact sperm parameters, but Coenzyme Q10 (CoQ10), as an antioxidant, protects sperm from such damage. CoQ10 antioxidant activity requires reduction by the NAD(P) oxidoreductase 1(NQO1) enzyme. This study investigated the assoc...

Down syndrome (DS) is the leading cause of intellectual disability, yet the factors contributing to its occurrence remain largely unknown. In this study, we investigated the genetic influence of the C677T variant in the methylenetetrahydrofolate reductase (MTHFR) gene, alongside maternal age, family history, and miscarriages, on the development of...

Identifying menopause-related breast cancer biomarkers is crucial for enhancing diagnosis, prognosis, and personalized treatment at that stage of the patient’s life. In this paper, we present a comprehensive framework for extracting multiomics biomarkers specifically related to breast cancer incidence before and after menopause. Our approach integr...

Elevated concentrations of reactive oxygen species (ROS) in the semen can lead to oxidative protein damage as they react with the amino acids' side chains in the protein, leading to the generation of carbonyl groups. This study aimed to investigate the effect of protein carbonyl (PC) concentration on sperm motility and the laboratory intracytoplasm...

Infertility is a common health problem that affects around 1 in 6 couples in the United States, where half of these cases are attributed to male factors. Genetics play an important role in infertility and it is estimated that up to 50% of cases are due to genetic factors. Despite this, many male infertility cases are still idiopathic. This study ai...

Objective: Poor ovarian response (POR) refers to a subnormal follicular response that leads to a decrease in the quality and quantity of the eggs retrieved after ovarian stimulation during assisted reproductive treatment (ART). The present study investigated the associations of multiple variants of the estrogen receptor 2 (ESR2) and follicle-stimu...

Sperm mitochondrial dysfunction causes the generation of an insufficient amount of energy needed for sperm motility. This will affect sperm fertilization capacity, and thus, most asthenozoospermic men usually require assisted reproductive techniques. The etiology of asthenozoospermia remains largely unknown. The current study aimed to investigate t...

The expansion of trinucleotide CGG repeats in the promoter of fragile X mental retardation 1 (FMR1) gene is associated with fragile X and fragile X associated tremor/ataxia syndromes. While the expansion of CGG repeats has been associated with such neuro/psychiatric diseases, the contraction of CGG repeats has been recently suggested as an indicati...

Male infertility is linked to some viral infections including human papillomavirus (HPV), herpes simplex viruses (HSV) and human immunodeficiency viruses (HIVs). Almost nothing is known about severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) effect on fertility. The possible risk factors of coronavirus disease 2019 (COVID-19) infecti...

Male infertility is linked to some viral infections including human papillomavirus (HPV), herpes simplex viruses (HSV), and human immunodeficiency viruses (HIVs), among others. Almost nothing is known about severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) effect on fertility. The possible risk factors of coronavirus disease 2019 (COVID-...

CAG trinucleotide repeats are coded for the polyglutamine tract in the N‐terminal of the androgen receptor (AR) gene which varies in normal individuals from 6 to 36 residues. In this study, we inspected the impact of the CAG repeats on the spermatogenic defects by measuring the size of AR‐CAG repeats length in a cohort of 260infertile and 169 ferti...

Male infertility is commonly associated with sperm abnormalities including asthenozoospermia. The molecular basis of asthenozoospermia was linked to mitochondrial DNA (mtDNA) mutations. The 4,977‐bp human mtDNA deletion is one of the most common mutations of spermatozoa and results in loss of about 33% of the mitochondrial genome. In this prelimina...

Background: Newborn ovary homeobox (NOBOX) gene plays a critical role in the transcriptional regulation of oocyte-specific genes. Previous studies have demonstrated a pathogenic effect of NOBOX variants on premature ovarian insufficiency (POI) patients. Poor ovarian response (POR) is a risk factor for POI. Therefore, genetic variants in the NOBOX...

Triple negative breast cancer (TNBC) is an aggressive and rapidly growing subtype of breast cancer characterized by the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) expression, rendering it resistant to targeted therapies. In the absence of molecular targets, the pathogenesis of TNB...

Persistent infection with high-risk human papillomavirus (HPV) is the leading cause of cervical cancer worldwide. In this study, we used immunohistochemical methods to investigate the prevalence of HPV among Jordanian women who visited local gynaecological clinics. Expression of HPV and p16 protein was analysed to assess the high-risk prevalence of...

Assisted reproductive technology is a common procedure which helps millions of couples who suffer fertility problems worldwide every year. Screening for genetic abnormalities prior to such procedure is very important to prevent the transmission of harmful genetic mutations to future generations. Microdeletions within the azoospermia factor (AZF) re...

Wolfram syndrome (WS) is a rare autosomal recessive neurodegenerative disorder characterized by the presentation of early onset type I diabetes mellitus and optic atrophy with later onset diabetes insipidus and deafness. WFS1 gene was identified on chromosome 4p16.1 as a gene responsible for the presentation of WS disorder given that most of the WS...

Chromosome segregation in meiosis is controlled by a conserved pathway that culminates in Separase-mediated cleavage of the α-kleisin, Rec8, leading to dissolution of cohesin rings. Drosophila has no rec8 gene and the absence of a known Separase target raises the question of whether Separase and its regulator Securin are important in Drosophila mei...

Infertility is a serious problem that affects between 10%-15% of couples all over the world in which half of these cases are attributed to males. Deletions in the azoospermia factor (AZF) of the Y chromosome have been investigated and shown to be associated with azoospermia and severe oligozoospermia in many populations. However, the percentages of...

The spindle assembly checkpoint (SAC) plays an important role in mitotic cells to sense improper chromosome attachment to spindle microtubules and to inhibit APC(Fzy)-dependent destruction of cyclin B and Securin; consequent initiation of anaphase until correct attachments are made. In Drosophila , SAC genes have been found to play a role in ensuri...