Orawan Suwantong

Mae Fah Luang University | MFU · Materials Science

The aim of this study was to fabricate and characterize nanoemulsion-loaded Garcinia cowa leaf extract (GC) for use as pharmaceutical and cosmeceutical products. The GC nanoemulsions were prepared by ultrasonication method. Properties of the GC nanoemulsions were investigated, including morphology, particle size, polydispersity index (PDI), zeta po...

Cannabidiol is a non-psychoactive cannabinoid isolated from the cannabis plant. Instead of intoxicating effects, Cannabidiol is known for its antioxidant, anti-inflammatory, and anti-anxiety effects. Being antagonist to CB1 and CB2 cannabinoid receptors, Cannabidiol binds to CB receptors through negative allosteric modulation; reduce binding affini...

Injectable self-healing hydrogels are attractive materials for use as wound dressings. To prepare such hydrogels, the current study used quaternized chitosan (QCS) to improve the solubility and antibacterial activity and oxidized pectin (OPEC) to introduce aldehyde groups for Schiff's base reaction with the amine groups from QCS. Self-healing hydro...

Semi-solid poly(vinyl alcohol) (PVA)-borax hydrogels containing a cannabidiol (CBD)/β-cyclodextrin (β-CD) inclusion complex were prepared and aimed for being used as wound management materials. The inclusion complex was prepared by the precipitation method and loaded within PVA semi-solid hydrogels which had various contents of CBD/β-CD inclusion c...

Quaternized chitosan (QCS) was blended with pectin (Pec) to improve water solubility and antibacterial activity of the hydrogel films. Propolis was also loaded into hydrogel films to improve wound healing ability. Therefore, the aim of this study was to fabricate and characterize the propolis-loaded QCS/Pec hydrogel films for use as wound dressing...

In daily life, people are often receiving minor cuts due to carelessness, leaving wounds on the skin. If wound healing is interrupted and the healing process does not finish, pathogens can easily enter wounds and cause infection. Liquid bandages are a fast and convenient way to help stop the bleeding of superficial wounds. Moreover, antibacterial a...

Semi-solid poly(vinyl alcohol) (PVA)-borax hydrogels containing a cannabidiol (CBD)/β-cyclodextrin (β-CD) inclusion complex were prepared and aimed for being used as wound management materials. The inclusion complex was prepared by the co-precipitation method and loaded within PVA semi-solid hydrogels which had various contents of CBD/β-CD inclusio...

In this study, porous natural rubber (NR) films loaded with silver nanoparticles (AgNPs) were prepared by the solvent casting method. The porous structure of the NR films was formed with hydrophilic substances such as glycerol, pectin or carrageenan. The film properties including morphology, mechanical test, hydrophilicity evaluation, water absorpt...

This study concentrated on developing quercetin/cyclodextrin inclusion complex-loaded polyvinyl alcohol (PVA) hydrogel for enhanced stability and solubility. Quercetin was encapsulated in hydroxypropyl-β-cyclodextrin (HP-β-CD) by the solvent evaporation method. The prepared quercetin/HP-β-CD inclusion complex showed 90.50 ± 1.84% encapsulation effi...

Acne vulgaris is an inflammatory skin condition of the pilosebaceous unit associated with the colonization of Cutibacterium acnes. One of the problems when using antibiotic creams is antibiotic molecules cannot penetrate the stratum corneum layer of the skin, which acts as a barrier for the molecules. To overcome this problem, a transdermal patch w...

The potential of a drug delivery system of the longan seed extract (LSE) incorporated in the alginate/chitosan (Alg/CS) beads has been studied. The LSE-loaded Alg/CS beads were prepared using the ionic gelation method via the interaction between protonated amino groups of CS and negatively charged carboxylic groups of Alg. Properties of the LSE-loa...

Treatment of chronic wounds requires good monitoring for the wound infection. One of the indicators for the infected wound is the change of pH values around the wound area. In this study, the visual pH sensor from Clitoria ternatea (CT) flower was used to indicate the pH value and evaluate the wound status. The CT extract contains anthocyanins whic...

The potential of chitosan nanoparticles (CNPs) loaded with essential oil (EO) incorporated into semi-solid PVA hydrogels for use in wound management was studied. Two types of essential oil were compared including clove essential oil (CEO) and turmeric essential oil (TEO). The EO-loaded CNPs were prepared by a two-step method; oil-in-water (o/w) emu...

Novel copper (II) ion‐imprinted polymers (Cu‐IIPs) were synthesized by bulk polymerization using 1 mmol of copper (II) sulfate pentahydrate as the template ion, 1 mmol of ethylenediaminetetraacetic acid (EDTA) as the ligand, and 4 mmol of 4‐vinyl pyridine (4‐VP) or methacrylic acid (MAA) as the functional monomer dissolved in a porogen of 20 mL of...

Gelatin (Gel) is a protein polymer obtained from the hydrolysis of collagen. Gel was used to prepare a wound dressing material loaded with asiaticoside (AC), a trisaccharide triterpene from Centella asiatica (L.) Urban commonly used in wound healing and dermis reconstruction. To improve water solubility, AC was formed into an inclusion complexation...

The aim of this study was to fabricate and characterize curcumin-β-cyclodextrin inclusion complex loaded sodium alginate/chitosan (CMx-loaded SA/CS) bilayer hydrogels for use as wound dressing materials. Various concentrations of calcium chloride (CaCl2) including 0.05, 0.10, and 0.20% w/v was directly added to SA layer for crosslinking hydrogels....

In this research, the cellulose sponges with curcumin-β-cyclodextrin inclusion complex (CMx) and chitosan (CS) were fabricated for use as wound dressings. 1-Allyl-3-methylimidazolium chloride (AMIMCl) ionic liquid as a green solvent was used for the fabrication of cellulose sponges. Due to the low aqueous solubility and low bioavailability of curcu...

In this study, alginate/gelatin (Alg/Gel) hydrogels containing silver nanoparticles (AgNPs) cross-linked with 1.0, 2.5, and 5.0% w/v calcium chloride (CaCl2) were fabricated using solvent casting method. The morphology and mechanical properties of Alg/Gel hydrogels containing AgNPs were observed by scanning electron microscopy and universal testing...

Here, we demonstrated the fabrication of a composite scaffold (chitosan [CS], collagen [Col], and hydroxyapatite [HA]) with the incorporation of encapsulated Cissus quadrangularis (CQ) extract for tissue engineering applications. First, the crude extract of CQ loaded nanoparticles were synthesized via double emulsion technique using polycaprolacton...

Recent advances in electrospinning are yielding intricate scaffolds for use in regenerative medicine. To explore the possibility of creating bioactive scaffolds with functional gene expression systems, electrospun gelatin mats bearing plasmid DNA (pDNA) polyplexes are explored. The pDNA is first condensed with a lipid-modified polyethylenimine (PEI...

Encapsulation of ginger (Zingiber officinale Roscoe) essential oil (GEO) in different amounts (i.e. 1%, 3%, and 5% v/v) within chitosan nanoparticles (CNPs) was studied. It was done by a two-step method: oil in water emulsion (O/W) and ionic gelation using sodium tripolyphosphate (STPP) as the crosslinking agent. GEO-loaded CNPs appeared to be more...

In this study, the injectable thermogelling hydrogels under physiological temperature were prepared using chitosan (CS) and silk fibroin (SF). These hydrogels were loaded with crude water extract of longan seed (WLS) to improve osteoblast differentiation and stimulate mineralization in bone cells. These hydrogels showed a sol-to-gel transition time...

Poly(l-lactic acid) (PLLA) scaffolds were prepared by a particulate leaching method using sodium chloride (NaCl) with the size of 300–425 µm as a particulate leaching. The PLLA/NaCl weight ratios were varied to be 1:8, 1:10, 1:12, and 1:15. The gentamicin sulfate (GS)-loaded PLLA-sericin hybrid scaffolds were prepared by immersion of the PLLA scaff...

Electrospun poly(l-lactic acid) (PLLA) fiber mats containing curcumin were prepared by electrospinning. Varying amounts of as-loaded curcumin at 0.2, 0.5, and 1.0% w/w (based on the weight of PLLA) were added in the PLLA solution. Results showed that the obtained fibers were smooth with no surface aggregates, indicating complete incorporation of cu...

The effects of the β-cyclodextrin (CD) complexation of curcumin (CUR) and the quaternization of chitosan (CS) on the properties of the blend films were studied. The quaternized chitosan containing curcumin (CUR-QCS) and the quaternized β-cyclodextrin grafted with chitosan containing curcumin (CUR-QCD-g-CS) were prepared. The CUR-QCS or CUR-QCD-g-CS...

In this study, hydrogels that were thermosensitive at body temperature were developed using chitosan (CS)/silk sericin (SS)/β-glycerophosphate (β-GP) loaded with longan seed extract (LE) for use in bone tissue engineering. These hydrogels were transformed into a gel at 37 °C within 10 min via interactions between CS and β-GP. The incorporation of S...

Bacterial cellulose (BC) nanofibers, with and without silane surface modification, were incorporated into self-reinforced poly(lactic acid) (SR-PLA) nanocomposites at 1 and 10 wt%. Disintegrated BC was combined with electrospun PLA fiber mats by film stacking and compression molding at 165 °C for 40 sec to obtain SR-PLA/BC hybrid films. The effect...

The prednisolone molecularly imprinted polymer nanoparticles (prednisolone‐MIPs) were prepared via precipitation polymerization. The synthetic conditions were performed by optimizing of functional monomers (acrylamide and methacrylic acid) and crosslinkers (ethylene glycol dimethacrylate and pentaerythritol triacrylate) with defined the synthetic r...

Copper(II) (Cu2+) ion‐imprinted polymers were prepared by suspension polymerization using 2.5 mmol of copper(II) sulfate pentahydrate as the template ion, 5 mmol of 4‐vinylpyridine and methacrylic acid as functional monomers, 20 mmol of ethylene glycol dimethacrylate as the cross‐linker, 1 mmol of 1,1′‐azobis(cyclohexanecarbonitrile) as the initiat...

The aim of this research study was to develop polyvinyl alcohol (PVA) hydrogels containing ciprofloxacin hydrochloride (CIP)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles as antibacterial materials capable of supporting the treatment of infected pressure ulcers. Either 6.25 mg, 39 mg, or 244 mg of CIP was loaded onto PLGA nanoparticles...

Gentamicin sulfate (GS)-loaded silk fibroin (SF)/gelatin (Gel) scaffolds were prepared by the freeze-drying method. The effect of blending ratios of SF and Gel (i.e., 0/100, 30/70, 50/50, 70/30, and 100/0) in the scaffolds on the morphology, pore size, compressive modulus, water swelling, weight loss, and release profiles was investigated. The pore...

Electrospinning has been extensively used to construct tissue‐engineered scaffolds because of its ability to provide the fibrous scaffold with structurally analogous to the naturally occurring protein in the extracellular matrix of native tissues. In addition, the modification of scaffolds with bioactive molecules is beneficial as this can create a...

Silk has been intensively studied for tissue engineering applications owning to its advantages of biodegradability, tunable mechanical properties, versatile processing, and biocompatibility. Silk, however, has few cell signaling domains, so silk alone might not be sufficient to use as the scaffolds for tissue engineering. Here, we demonstrated the...

Interactions between cells and substrates play an important role in tissue development during the process of tissue regeneration. Substrates that mimic the surface topography and chemical composition of the extracellular matrix (ECM) lead to enhanced cellular interactions. Electrospinning can easily produce aligned fibrous substrates with an archit...

Today, the development of materials has to involve the consideration of environmental responsibility and sustainability. Therefore, more environmentally friendly products, which are biodegradable and derived from renewable resources, have been extensively established. However, in widespread applications, most bio-based polymers when used alone pres...

The embedding of silver nanoparticle (nAg)-containing calcium alginate (CaAlg) beads in gelatin scaffolds was aimed to reduce the burst release and prolong the release of silver (Ag+) ions for a long period of time. The reduced sizes of the nAg-containing CaAlg beads were prepared by an emulsification/external gelation method. The diameter of these...

Polycaprolactone (PCL) is a biodegradable polyester emerging into biomedical applications because of its biodegradability, biocompatibility, chemical stability, thermal stability and good mechanical properties. Electrospinning is a versatile method using electrostatic forces for fabricating continuous ultrafine fibers that offer various advantages...

Electrospinning is an efficient process for fabrication of polymeric ultrafine fibers with diameters ranging from sub-micrometer to nanometer. It involves the application of a strong electric field across a conductive capillary attaching to a polymer liquid-containing reservoir and a collector. The obtained ultrafine fibers exhibit several interest...

The silver nanoparticles (AgNPs)-loaded calcium alginate beads embedded in gelatin scaffolds were developed to sustain and maintain the release of silver (Ag+) ions over an extended time period. UV irradiation technique was used to reduce the Ag+ ions in alginate solution to AgNPs. The average AgNPs sizes ranged between ~20 and ~22 nm. The AgNPs-lo...

Silver nanoparticles (AgNPs)-loaded alginate beads embedded in gelatin scaffolds were successfully prepared. The AgNPs-loaded calcium alginate beads were prepared by electrospraying method. The effect of alginate concentration and applied voltage on shape and diameter of beads was studied. The diameter of dry AgNPs-loaded calcium alignate beads at...

Poly(l-lactic acid) (PLLA) fiber mats containing two types of crude Garcinia mangostana Linn. (GM) extract [i.e., dichloromethane extract (dGM) and acetone extract (aGM)] were successfully prepared by electrospinning process. Both the neat and the GM-loaded PLLA fibers were smooth, with the average diameters ranging between 0.77 and 1.14 μm. The re...

Electrospun poly(L-lactic acid) (PLLA) fiber mats containing an acetone crude extract of Garcinia dulcis Roxb. (GD) at either amount of 30% or 50% (based on the weight of PLLA) were successfully prepared by electrospinning. Both the neat and the GD-loaded PLLA fibers were smooth, with the average diameters of the fibers ranged between 0.60 and 1.13...

Poly(L-lactic acid) (PLLA) fiber mats containing a crude extract of Garcinia cowa Roxb. (GC) were prepared by electrospinning. The extract was introduced at a level of either at 30 or 50 % with respect to the weight of PLLA. The fibers of both the neat and the GC-loaded PLLA fibers were smooth, with average diameters of 0.80–1.13 μm. The characteri...

Electrospinning has been recognized as a versatile method for the fabrication of continuous ultrafine fibers using electrical forces. Various natural and synthetic polymers have been successfully electrospun into non-woven mats or oriented fibrous bundles with high porosity and large surface areas. Despite the numerous reports on the production of...

Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The ζ-potential continuously increased with higher polymer:siRNA weight ratio, and the ζ-potential of lipid-modified polymers:siRNA complexes were higher than PEI2 at all ratios. At polymer:siRNA ratio of...

In the present contribution, ultra-fine zein fibers were successfully prepared by electrospinning. The diameters of these fibers ranged between 0.32 and 1.10 μm. Cross-linked electrospun zein fiber mats were prepared by adding varying quantities of glyoxal aqueous solution (i.e., 0, 5, 10 and 15% v/v based on the volume of the zein solution) into t...

Ultra-fine cellulose acetate (CA; M(w) approximately 30,000 Da; degree of acetyl substitution approximately 2.4) fiber mats containing either asiaticoside [from the plant Centella asiatica (L.); either in the form of a crude extract (CACE) or pure substance (PAC)] or curcumin (CM; from the plant Curcuma longa L.) were successfully prepared. The pro...

Chitosan or poly(N-acetyl-d-glucosamine-co-d-glucosamine) with a degree of deacetylation of about 85% was fabricated into nanofibrous membranes by electrospinning from 7% w/v chitosan solution in 70:30 v/v trifluoroacetic acid/dichloromethane solvent system. The obtained fibers were smooth without the presence of beads. The diameters of the individ...

This study was conducted in order to develop amphiphilic, low molecular weight polymeric carriers for nonviral gene delivery. Caprylic, myristic, palmitic, stearic, oleic and linoleic acids were grafted onto the 2 kDa polyethylenimine (PEI) and properties critical for gene delivery were investigated using 293T and bone marrow stromal cells. The ext...

Synopsis Electrospinning is a process used to produce ultrafine fibers with diameters in the nanometer range. Electrospun fiber mats have high potentials for biomedical uses, due to their high surface area and ease of drug incorporation into the fibers. They can be used as carriers for drug delivery and can enhance drug release and skin permeabilit...

To make polycaprolactone (PCL) more suitable for tissue engineering, PCL in the form of electrospun fibrous scaffolds was first modified with 1,6-hexamethylenediamine to introduce amino groups on their surface. Various biomolecules, i.e., collagen, chitosan, and Gly-Arg-Gly-Asp-Ser (GRGDS) peptide, were then immobilized on their surface, with N,N'-...

a b s t r a c t Ultra-fine cellulose acetate (CA; M w z 30,000 Da; degree of acetyl substitution z 2.4) fiber mats con-taining asiaticoside (AC) from the plant Centella asiatica L. either in the form of pure substance (PAC) or a crude extract (CACE) were fabricated by electrospinning. Incorporation of either PAC or CACE (40 wt.% based on the weight...

Tooth extraction induces residual ridge resorption which impairs function and aesthetic of dental prostheses. This study aimed at developing new bone scaffolds to be used in a tooth socket for preserving bone mass from the residual ridge resorption. Scaffolds were fabricated from poly(1,4-butylene succinate) extended with 1,6-diisocyanatohexane (PB...

Electrospinning is a process used to produce ultrafine fibers with diameters in the nanometer range. Electrospun fiber mats have high potentials for biomedical uses, due to their high surface area and ease of drug incorporation into the fibers. They can be used as carriers for drug delivery and can enhance drug release and skin permeability. The ai...

Ultra-fine cellulose acetate (CA; Mw ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) fiber mats containing curcumin from the plant Curcuma longa L., widely known for its anti-tumor, antioxidant, and anti-inflammatory properties, were fabricated, for the first time, from the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) containin...

The in vitro responses of Schwann cells (RT4-D6P2T, a schwannoma cell line derived from a chemically induced rat peripheral neurotumor) on various types of electrospun fibrous scaffolds of some commercially available biocompatible and biodegradable polymers, i.e., poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), p...

In the present contribution, the potential for use of the ultrafine electrospun fiber mats of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as scaffolding materials for skin and nerve regeneration was evaluated in vitro using mouse fibroblasts (L929) and Schwann cells (RT4-D6P2T) as reference cell lines. Comp...

Electrospinning of poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and their blends was first carried out in chloroform at 50 °C on a stationary collector. The average diameter of the as-spun fiber from PHB and PHBV solutions decreased with increasing collection distance and increased with increasing solution con...