Ophélie Menant- PhD
- Post-doctoral researcher at TEAGASC - The Agriculture and Food Development Authority
Ophélie Menant
- PhD
- Post-doctoral researcher at TEAGASC - The Agriculture and Food Development Authority
Post-doctoral researcher at TEAGASC - The Agriculture and Food Development Authority
About
12
Publications
5,660
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Introduction
Current institution
Publications
Publications (12)
Out-of-season lambing is based in productivity aims, but also modifies the basic physiology of the ewe. It might affect ewes and lambs’ behaviours at birth, their bond, the process of lambs independence from their mothers and their response at weaning. This study aimed to compare the ewe-lamb bond from birth to artificial weaning in out-of-season l...
Access full-text: https://authors.elsevier.com/c/1cbE9_3qj2Tw35 Brushing produces a relaxing effect and improves the welfare of ewes and lambs and the human-animal relationship. Lambs gently handled during rearing are less fearful to humans in their adulthood, but there are few studies on the development of a positive human-animal relationship in a...
Conceiving during the early postpartum period, when ewes are investing energy resources nursing an offspring is energetically demanding. Moreover, it requires artificial weaning during ewe gestation, which induces a strong stress response. All this together can impact fetal growth and lambs’ mortality rate. The aim of the study was to determine if...
In extensive systems, most of the lambs' deaths are related to their low vitality at birth, and an inadequate ewe-lamb bond, leading to lambs' hypothermia. In this context, the aim of this study was to determine if lambs' rectal and body surface temperatures were related to the ewes and lambs behaviours at birth and during a separation-reunion test...
Sensory substitution refers to a process whereby an agent, by means of a removable specialized instrumentation, becomes capable of exploiting an available sensory modality in order to perceive properties of the environment which are normally accessible by means of a different modality. We describe a situation of visual-auditory sensory substitution...
The periaqueductal gray (PAG) is less referred in relationship with emotions than other parts of the brain (e.g. cortex, thalamus, amygdala), most probably because of the difficulty to reach and manipulate this small and deeply lying structure. After defining how to evaluate emotions, we have reviewed the literature and summarized data of the PAG c...
The periaqueductal gray (PAG) is a mesencephalic brain structure organised in subdivisions with specific anatomical connections with the rest of the brain. These connections support the different PAG functions and especially its role in emotion. Mainly described in territorial and predatory mammals, examination of the PAG connections suggests an op...
La question des émotions des animaux d’élevage s’inscrit dans la volonté d’améliorer les conditions d’élevage en prenant en compte leur bien-être. La genèse des émotions repose sur la capacité cognitive des individus à percevoir, évaluer et réagir à leur environnement. La capacité de perception met en jeu différentes sensorialités et participe à la...
The periaqueductal gray (PAG) is a mesencephalic brain structure involved in the expression of numerous behaviours such as maternal, sexual and emotional. Histological approaches showed the PAG is composed by subdivisions with specific cell organisation, neurochemical composition and connections with the rest of the brain. The comparison of studies...
In this work, we investigated the link between the blood-oxygen-level
dependant (BOLD) effect observed using functional magnetic resonance imaging (fMRI) and the neurite density inferred from the Neurite Orientation Dispersion and Density Imaging (NODDI) model in some well-known lateralized cortical areas. We found a strong colocalization between t...
This preliminary experiment aims to develop a conditioning protocol to study humananimal
relationship in piglets. One week after weaning, five groups of three piglets were
subjected to a conditioning situation repeated 11 times over 3 days: a human entered the
home pen and sat passively in a corner for 5 minutes (P) or delivered a stimulus each
tim...
Questions
Question (1)
I am doing IHC on free floating sheep brains slides. For pratical reasons after cuting the slides with a cryostat, we conserved the brain slides at -20ºC in a cryoprotectant solution ( Sucrose , Ethylene glycol, Buffer ).
I did a c-Foc protocol which did not work. This prococtol was already used on the same brains (but other parts) with the same anti-bodies references but the slides were conserved only at +4ºC in PBS-A solution. On the later condition, the protocol worked well. It is seems that the cryoprotectant solution interferes with the IHC. To solv this problem I did 7 wasking in TBS-A at +4ºC with agutation distributed over 24 hours, without succes.
Is someone has already had a problem with the cryoprotectant solution ? If yes, how did you solve it ?
Thanks for your help
