Ondrej Gahura

Ondrej Gahura
Institute of Parasitology Biology Centre, Czech Academy of Science

22.61
 · 
PhD
About
16
Research items
1,585
Reads
162
Citations
Research Experience
Sep 2013 - Sep 2017
MRC Mitochondrial Biology Unit
Position
  • Visiting Postdoc
Jan 2013
Institute of Parasitology Biology Centre, Czech Academy of Science
Position
  • Postdoctoral Associate
Oct 2006 - Apr 2012
Faculty of Science, Charles University in Prague
Position
  • Research Assistant
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Research
Research items (16)
Article
F1-ATPase is a membrane-extrinsic catalytic subcomplex of F-type ATP synthase, an enzyme that uses the proton motive force across biological membranes to produce adenosine triphosphate (ATP). The isolation of the intact F1-ATPase from its native source is an essential prerequisite to characterize the enzyme's protein composition, kinetic parameters...
Article
Hydrolysis of ATP by the mitochondrial F‐ATPase is inhibited by a protein called IF1. In the parasitic flagellate, Trypanosoma brucei, this protein, known as TbIF1, is expressed exclusively in the procyclic stage, where the F‐ATPase is synthesizing ATP. In the bloodstream stage, where TbIF1 is absent, the F‐ATPase hydrolyzes ATP made by glycolysis...
Conference Paper
The sophisticated rotational mechanism of ATP synthesis coupled to the proton translocation across biological membranes imposes significant constraint to evolutionary diversification of F-type ATP synthases. The compositional and structural variability of these enzymes is restricted to the regions not engaged in the proton pumping, torque transmiss...
Article
Full-text available
Author summary Human African Trypanosomiasis (HAT) is a life-threatening infectious disease caused by the protozoan parasite, Trypanosoma brucei. Current treatments suffer from low efficacy, toxicity issues and complex medication regimens. Moreover, an alarming number of these parasites are demonstrating resistance to current drugs. For these reaso...
Data
List of oligonucleotides used in this study. (DOCX)
Article
Full-text available
The structures and functions of the components of ATP synthases, especially those subunits involved directly in the catalytic formation of ATP, are widely conserved in metazoans, fungi, eubacteria, and plant chloroplasts. On the basis of a map at 32.5-Å resolution determined in situ in the mitochondria of Trypanosoma brucei by electron cryotomograp...
Article
Full-text available
The F-ATPases (also called the F1Fo-ATPases or ATP synthases) are multi-subunit membrane-bound molecular machines that produce ATP in bacteria and in eukaryotic mitochondria and chloroplasts. The structures and enzymic mechanisms of their F1-catalytic domains are highly conserved in all species investigated hitherto. However, there is evidence that...
Article
Full-text available
Splicing in S. cerevisiae has been shown to proceed cotranscriptionally, but the nature of the coupling remains a subject of debate. Here, we examine the effect of nineteen complex-related splicing factor Prp45 (a homolog of SNW1/SKIP) on cotranscriptional splicing. RNA-sequencing and RT-qPCR showed elevated pre-mRNA levels but only limited reducti...
Article
Full-text available
The mitochondrial (mt) FoF1-ATP synthase of the digenetic parasite, Trypanosoma brucei, generates ATP during the insect procyclic form (PF), but becomes a perpetual consumer of ATP in the mammalian bloodstream form (BF), which lacks a canonical respiratory chain. This unconventional dependence on FoF1-ATPase is required to maintain the essential mt...
Article
Full-text available
We reconstruct the historical biogeography of cichlid fishes endemic to the trans-Andean region of NW South America. DNA sequences were used to study historical biogeography of the cichlid genera Andinoacara (Cichlasomatini) and Mesoheros (Heroini). Two event-based methodological approaches, parsimony-based Statistical Dispersal-Vicariance Analysis...
Article
Full-text available
Circadian clocks generate endogenous rhythms in most organisms from cyanobacteria to humans and facilitate entrainment to environmental diurnal cycles, thus conferring a fitness advantage. Both transcriptional and posttranslational mechanisms are prominent in the basic network architecture of circadian systems. Posttranscriptional regulation, inclu...
Article
Full-text available
Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3′ splice site (3′ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure...
Article
Human transcription co-regulator SNW1/SKIP is implicated in the regulation of both transcription elongation and alternative splicing. Prp45, the SNW/SKIP ortholog in yeast, is assumed to be essential for pre-mRNA processing. Here, we characterize prp45(1-169), a temperature sensitive allele of PRP45, which at permissive temperature elicits cell div...