Omoanghe S. Isikhuemhen

Omoanghe S. Isikhuemhen
North Carolina Agricultural and Technical State University | NC A&T · Department of Natural Resources and Environmental Design

PhD

About

159
Publications
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1,676
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Publications

Publications (159)
Article
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Commercial cultivation of Ganoderma species found in Nigeria does not exist. Four Ganoderma isolates (YCT-BKS, YCT-Q2, YCT-Q14, and YCT-Q18) collected in Lagos were tested for mycelia growth and cultivation in sawdust-based substrates. Internal transcribed spacer sequences (ITS1 and ITS4) from three isolates upon a GenBank BLAST search gave DNA seq...
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Silver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi due to silver exposure are scanty. This present w...
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Quality spawn, which is also dependent on grain composition, is a critical factor that must be optimized to achieve successful and profitable mushroom farming. The characteristics of grain spawn composition (Factor A) and two microclimatic fruiting conditions (Factor B) were studied in the cultivation of Pleurotus ostreatus . Eight different grain...
Article
Agaricus subrufescens has emerged as an important culinary-medicinal mushroom over the last decades. Efforts have been dedicated to upgrade the A. subrufescens productive process via strain selection and cultivation scaling-up. However, little is known on the influence of those variables on the metabolite profiles and nutraceutical properties of th...
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The integrity of fruit bodies of oyster mushrooms is a significant factor that affects their storability. The natural flora of bacterial on fresh mushrooms start to grow when mushroom fruit body tissues are injured during packaging into polystyrene foam trays (PFT). Various cluster sizes of mushrooms and the standard size PFT used by growers in Ukr...
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Metal nanoparticles used as antifungals have increased the occurrence of fungal-metal interactions. However, there is a lack of knowledge about how these interactions cause genomic and physiological changes, which can produce fungal superbugs. Despite interest in these interactions, there is limited understanding of resistance mechanisms in most fu...
Article
Pleurotus tuber-regium has been reported to lower dexamethasone-induced ocular hypertension. Effects of P. tuber-regium and 0.005% latanoprost on the circadian intraocular pressure (IOP) cycle in cats were investigated for a possible phase difference. The research was conducted in three phases. In phase 1, the baseline diurnal IOP of 12 cats was de...
Article
The milky white mushroom, Calocybe indica Purkay & A. Chandra, is a tropical species not commercially cultivated in Europe. The influence of casing and scratching treatments on productivity, morphological characteristics, and nutritional contents were investigated during the cultivation of C. indica on locally available substrates. The highest tota...
Article
Previous studies of the lung microbiome have focused on characterizing the community and attempts to understand the role of community membership concerning disease or exposures such as cigarette smoke. However, we still lack an understanding of two critical aspects of the lung microbiome: the origin of the community members and their fate. As we co...
Article
A batch culture technique was used to evaluate dry matter (DM) digestibility of corn stover (Zea mays L.) and kudzu, Pueraria montana (Lour.) Merr. after solid-state fermentation (SSF) with a white-rot fungus, Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. Five dietary treatments consisting of mixtures of corn stover (C) and kudzu (K) in varying ratio...
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Albino Grifola frondosa (Dicks.) Gray “maitake” mushrooms (described as G. albicans Imazeki and then placed in synonymy with G. frondosa) are particularly rare, and the few pertinent records are not treated in scientific publications. A field investigation carried out in Sicily (Italy) led to the collection of an unusual white Grifola specimen at t...
Article
Maintaining farm viability, especially during the post tobacco era, has been very challenging for North Carolina farmers experiencing market failure for their produce. Farmers in North Carolina are searching and willing to try new crops that have the potential to keep their farms in business and remain profitable. Industrial hemp seems to check all...
Article
Chicken intestinal microbiomes are known to enhance chicken health and increase production. Identifying natural supplements that shift bacteria communities in favor of beneficial bacteria is critical. This study is a preliminary investigation of the use of medicinal mushrooms nutritional supplements (MSs) including Lentinus edodes, Ganoderma lucidu...
Article
Agaricus bisporus, also known as the white button mushroom or champignon, is the most cultivated mushroom species worldwide. In addition to its favorable nutrient profile, it contains a number of compounds with antioxidant, antibacterial, anti-inflammatory, antitumor, and immunomodulatory activities. Waste biomass is a secondary product obtained fr...
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The beneficial properties of mushrooms’ bioactive compounds indicate their potential for use as performance-enhancing natural additives for livestock animals. A study was undertaken to evaluate the effects of diets supplemented with mushroom myceliated grains (MMGs) fed to dairy ewes on intestinal parasite load, milk production, milk fatty acid (FA...
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Background The lack of good production practices and probable post-fermentation contaminations contribute to bacterial pathogen load in ugba, a fermented food made from African oil been seeds (Pentaclethra macrophylla Benth). Some of these bacteria are not easily detected using standard culturing techniques. The study used molecular-based approache...
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Higher Basidiomycetes and Ascomycetes mushrooms possess various immunological and anticancer properties. They also offer important health benefits and exhibit a broad spectrum of pharmacological activities including antibacterial, antifungal, antiviral, cytotoxic, immunomodulating, anti-inflammatory, antioxidative, antiallergic, antidepressive, ant...
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Lentinus squarrosulus Mont. is an emerging tropical white rot basidiomycete, with nutritional and medicinal benefits. Low levels of commercial cultivation of the mushrooms limit their availability for use as food and medicine. Mycelia from submerged fermentation are a suitable alternative to the mushroom from L. squarrosulus. Three strains, 340, 33...
Article
Auricularia auricula (St. Aman's) Berk was cultivated on Mansonia altissima sawdust with various additives (Brewer's grain (BG), Corn chaff (CC), Oil palm fibre (OPF), Sorghum bicolor chaff (SC) and Wheat bran (WB) at different percentages (0%, 5%, 10% and 20%). The study was carried out to determine the effect of additives on the performance of th...
Article
In this study, culture conditions were optimized to improve lovastatin production by Omphalotus olearius, isolate OBCC 2002 using statistical experimental designs. The Plackett-Burman design was used to select important variables affecting lovastatin production. Accordingly, glucose, peptone, and agitation speed were determined as the variables tha...
Article
This study was undertaken to investigate the effect of 50% ethanol extract from the sclerotium of Pleurotus tuberregium (Fr.) Sing, an edible mushroom, on hepatocellular integrity of albino rats treated with carbon tetrachloride (CCl4). Activities of hepatic enzymes; alkaline phosphatase (ALP), alanine transaminase (ALT), and aspartate transaminase...
Article
Although lovastatin production has been reported for different microorganism species, there is limited information about lovastatin production by basidiomycetes. The optimization of culture parameters that enhances lovastatin production by Omphalotus olearius OBCC 2002 was investigated, using statistically based experimental designs under solid sta...
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Canola crop is rich in plant biomass. It is considered a major cash crop in North America and a po-tential source for biofuel. We evaluated six strains of white rot basidiomycetes under solid state fermentation (SSF) for their potentials to secrete oxidative and hydrolytic enzymes to biodegrade canola plant biomass (CPB), and release sugars. Fuscop...
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This study examined the impact of shiitake mushroom extract (ME) on the viability and α/β enzyme activity of Bifidobacterium breve ATCC 15701 and Lactobacillus reuteri DSM20016 in yoghurt during refrigerated storage. The presence of ME significantly enhanced the viability of both strains. L. reuteri DSM20016 exhibited better stability in yoghurt th...
Article
This study examined the impact of shiitake mushroom extract (ME) on the viability and α/β enzyme activity of Bifidobacterium breve ATCC 15701 and Lactobacillus reuteri DSM20016 in yoghurt during refrigerated storage. The presence of ME significantly enhanced the viability of both strains. L. reuteri DSM20016 exhibited better stability in yoghurt th...
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A study was conducted to evaluate the effects of different incubation time and level of inclusion of Fungus Myceliated Grain (FMG) in diets of broiler chickens. The nine different dietary treatments were as follows: (1) Control-No-FMG (2) 5% FMG-14 d (3) 10% FMG-14 d (4) 5% FMG-28 d (5) 10% FMG-28 d (6) 5% FMG-42 d (7) 10% FMG-42 d (8) 5% FMG-56 d...
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Beta-glucosidase is among the suite of enzymes pro-duced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activi-ties and kinetic properties of β-glucosidase from se-venteen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squ...
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An experiment was conducted to evaluate the feeding of four mushrooms separate and combined via Fungus Myceliated Grain (FMG) on broiler chicken performance after an Eimeria challenge at 14 days of age. Male broilers (n-180) were randomly allocated to 1 of 12 treatments, replicated 3 times with 5 chicks each in a battery cage system for 28 days as...
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Lentinus squarrosulus Mont., a high temperature tolerant white rot fungus that is found across sub-Saharan Africa and many parts of Asia, is attracting attention due to its rapid mycelia growth and potential for use in food and biodegradation. A solid state fermentation (SSF) experiment with L. squarrosulus (strain MBFBL 201) on cornstalks was cond...
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Eleven corticoid and three poroid fungi have been isolated from Latvian cultural monuments and evaluated for their potential to produce wood-degrading enzymes. In submerged fermentation of mandarin peels, notable intergeneric and intrageneric differences were revealed with regard to the extent of hydrolase and oxidase activity. The xylanase activit...
Chapter
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The relationships among species within Morchella and Macrolepiota were studied using nrDNA ITS and EF-1α sequences from 50 samples belonging to genera Gyromitra, Distiotis, Morchella, and Verpa; and 58 samples belonging to genera Macrolepiota, Leucoagaricus, and Chlorophyllum species from different geographical areas. Maximum parsimony, maximum lik...
Article
The capacity of white rot fungus, Pleurotus ostreatus, to biodegrade naphthalene present in sandy-clay-loam soil samples at concentrations of 50, 100, 200, and 500 mg=L was investigated experimentally. Using high-pressure liquid chromatography (HPLC), triplicate samples of liquid extracts from both fungus-inoculated and control (noninoculated) soil...
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Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight...
Article
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Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight...
Article
Auricularia auricula (St.Amans), a white rot fungus, was cultivated on Mansonia altissima (A. Chev) sawdust supplemented with 0,5, 10, and 20% brewer’s grain (BG), corn chaff (CC), oil palm fibre (OPF), Sorghum bicolor chaff (SC) and wheat bran (WB). Sawdust supplemented with 20% WB showed the fastest mycelial colonization (9 days), and density. Ho...
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An experiment was conducted to investigate Fungus Myceliated Grain (FMG) feed inclusion strategies for broilers and the effects of this feed on natural Eimeria oocyst excretion and bird performance. Two hundred ninety-four straight-run day-of-hatch broiler chicks were obtained from a local commercial hatchery and randomly distributed in seven treat...
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The objective of this study was to investigate the effect of the mushroom extract on the growth of lactic acid bacteria and bifidobacteria. Lactobacillus reuteri CF2-7F, L. reuteri DMS20016, Bifidobacterium breve and B. adolescentis were tested with different concentrations (0, 1, 2, and 4% w/v) of mushroom extract. Bacterial growth was monitored e...
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An experiment was conducted to determine if dietary Fungus Myceliated Grain (FMG) as an alternative to other coccidiosis control measures would result in oocyst reduction in the presence of a field strain Eimeria challenge during grow out. A total of 144 broiler chicks were assigned to 8 treatment groups as follows: 1) Control (no coccidiosis prote...
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This study investigated the effect of inoculation of white rot fungus, Pleurotus ostreatus, incubation time, and levels of contamination on anthracene degradation in contaminated soil over a 90-day period. Control samples were set up to compare rates of degradation at different levels of anthracene contamination in soil. Using HPLC, extracts from s...
Article
ABSTRACT: Lentinus squarrosulus Mont. is an important tropical edible and medicinal mushroom distributed across Sub-Saharan Africa and most parts of Asia. Intrastock mating study was performed using single spore isolates (ssi) from a wild strain (MBFBL 201). Mycelia growth and morphology characterization indicated that milled corn straw agar (CSA)...
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An experiment was conducted to investigate the effects of Fungus (Lentinula edodes) Myceliated Grain (FMG) supplementation on production performance, leukocyte levels and growth of Bifidobacteria and Salmonella populations in broiler chickens. A total of 240 broiler chickens were kept in floor pens and fed a corn and soybean meal based diet with ad...
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Small farms represent a significant proportion of the total U.S farms, and current trends in agriculture pose new challenges for their viability and survival. To meet such challenges, there is a need for introducing high-value non-traditional alternative enterprises. The study reported here explored goat, organic, and mushroom farming as potential...
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Two experiments were conducted to evaluate the administration of mushroom extract on bifidobacteria and Salmonella populations in broiler chicken feces. In the first experiment, a total of 100 female broiler chicks were assigned to 1 of the following treatment conditions, with 20 chicks per treatment: 1) control (no additive), 2) mushroom extract,...
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Molting in poultry is used to rejuvenate hens for a second or third laying cycle. Feed withdrawal was once the most effective method used for molt induction; however, it has being phased out due to food safety and animal welfare concerns. This study evaluated the utilization of fungus myceliated grain as a safe and effective alternative for inducin...
Article
Fungi are important in terrestrial decay processes. However, fungi associated with organic decay during composting are still not well known. In this study culture-independent methods were used to identify fungi associated with composting organic municipal wastes to gain a better understanding of the diversity of fungi associated with this process....
Article
Solid waste from anaerobic digestion of litter from the commercial production of broiler chickens has limited use as fertilizer. Its disposal is a major problem for digester operators who are seeking alternative use for anaerobic digester solids, also referred to as solid waste (SW). The use of SW as substrates for the cultivation of Pleurotus ostr...
Article
Genitourinary Candida spp isolates (n = 595) represented by C. glabrata, C. tropicalis, C. guilliermondii, C. albicans, C. pseudotropicalis, C. parapsilosis and C. stellatoidea were microscopically examined to determine their cell shapes, budding types and morphological responses to different pH values. Four cell shapes (spherical, oval, oval/spher...
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Cerrena unicolor HAI 1095 lectin from vegetatively grown mycelium was isolated and characterized. Two N-acetyl-D-galactosamine- and one galactose-specific lectin fractions were purified from the mycelia of C. unicolor by a procedure consisting of ammonium sulfate precipitation, gel filtration on a Toyopearl HW-55F column, affinity chromatography an...
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An experiment was conducted to evaluate the effects of mushroom and pokeweed extract alone or in combination with alfalfa meal on Salmonella spp. population, egg production, and weight loss in laying hens during a 10-d molting period. The trial used 54 active laying hens approximately 77 wk of age that were naturally infected with Salmonella spp. T...
Article
Productivity and nutritional contents in culinary-medicinal oyster mushroom Pleurotus ostreatus strain MBFBL67 cultivated on substrates containing anaerobic digester solid waste (SW) from poultry litter and wheat straw, with or without millet, were studied. Among the seven different combinations tested, a substrate combination consisting of 10 SW,...
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The degradation and utilization of solid waste (SW) from anaerobic digestion of poultry litter by Agrocybe aegerita was evaluated through mushroom production, loss of organic matter (LOM), lignocellulolytic enzymes activity, lignocellulose degradation and mushroom nutrients content. Among the substrate combinations (SCs) tested, substrates composed...
Article
This study was conducted to test the efficacy of an indigenous white rot fungus Lentinus squarrosulus in degrading engine oil in soil. Flasks containing sterilized garden soil (100 g) moistened with 75% distilled water (w/v) were contaminated with engine oil 1, 2.5, 5, 10, 20 and 40% w/w concentrations, inoculated with L. squarrosulus and incubated...
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The antagonistic/antifungal activities (AA/AFA) of the higher Basidiomycetes medicinal mushroom Pleurotus tub erregium were studied in a dualculture experiment using potato-dextrose agar at two different temperatures (26°C and 30°C) against phytopathogens(Fusarium culmorum, Bipolaris sorokiniana) and their antagonists (Trichoderma harzianum, T. asp...
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A study was conducted to evaluate the effect of combined Shiitake mushroom (Lentinus edodes) extract with probiotics (PrimaLac) on the growth and health of broiler chickens. In trial 1, 540 d-of-hatch chicks were randomly assigned to 6 treatment groups, replicated 3 times, with 15 males and 15 females per pen for 3 wk. Dietary probiotics and mushro...