Olivier Moralès

Olivier Moralès
Institut de Biologie de Lille | biologie lille · CNRS UMR8161 - Mécanismes de la tumorigenèse et thérapies ciblées

PhD, HDR

About

70
Publications
9,361
Reads
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1,060
Citations
Citations since 2016
26 Research Items
693 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Additional affiliations
January 2020 - present
Institut de Biologie de Lille
Position
  • Research Associate
Description
  • From basic OncoImmunology to the development of Immunotherapies and Phtodynamic Therapy protocols
December 2003 - January 2020
Institut de Biologie de Lille
Position
  • Research Associate
Education
October 2013 - December 2014
Université de Lille
Field of study
  • OncoImmunology
October 2005 - December 2009
Ecole Pratique des Hautes Etudes
Field of study
  • Immunology, Oncology and Virology

Publications

Publications (70)
Article
Full-text available
Photodynamic Therapy (PDT) relies on local or systemic administration of a light-sensitive dye, called photosensitizer, to accumulate into the target site followed by excitation with light of appropriate wavelength and fluence. This photo-activated molecule reacts with the intracellular oxygen to induce selective cytotoxicity of targeted cells by t...
Article
Full-text available
Changes in gene expression are increasingly used to evaluate the effects of exposure to environmental agents. Housekeeping genes (Hk) are essential in these analyzes as internal controls to normalize expression levels assessed in real-time PCR (RT-PCR). Ideal Hk genes (i) are constitutively expressed; (ii) do not respond to external stimuli and (ii...
Article
Full-text available
The development of immunotherapy has recently modified the anti-tumor therapeutic arsenal; particularly, immune checkpoint inhibitors have led to a significant increase in overall survival. The current challenge is now to select good responder patients by identifying early biomarkers to propose therapeutic combinations that potentiate the efficacy...
Article
Full-text available
Nasopharyngeal carcinoma (NPC) is a malignant tumour of the head and neck affecting localised regions of the world, with the highest rates described in Southeast Asia, Northern Africa, and Greenland. Its high morbidity rate is linked to both late-stage diagnosis and unresponsiveness to conventional anti-cancer treatments. Multiple aetiological fact...
Article
Full-text available
Often discovered at an advanced stage, ovarian cancer progresses to peritoneal carcinoma, which corresponds to the invasion of the serosa by multiple tumor implants. The current treatment is based on the combination of chemotherapy and tumor cytoreduction surgery. Despite the progress and standardization of surgical techniques combined with effecti...
Article
Surgical management of peritoneal metastases raises the problem of the theoretical spread of the entire peritoneal surface. Intraperitoneal photodynamic therapy (IntraPDT) has been limited by the lack of specificity of photosensitizers (PS) and difficulties to bring light into the abdominal cavity. Recent data in ovarian cancer may give development...
Article
Full-text available
To date, pancreatic adenocarcinoma (ADKP) is a devastating disease for which the incidence rate is close to the mortality rate. The survival rate has evolved only 2–5% in 45 years, highlighting the failure of current therapies. Otherwise, the use of photodynamic therapy (PDT), based on the use of an adapted photosensitizer (PS) has already proved i...
Preprint
Full-text available
Background: Changes in gene expression are increasingly used to evaluate the effects of exposure to environmental agents. Housekeeping genes (Hk) are essential in these analyzes as internal controls to normalize expression levels assessed in real-time PCR (RT-PCR). Ideal Hk genes (i) are constitutively expressed; (ii) do not respond to external sti...
Article
Full-text available
Background: The role of regulatory T cells (Tregs) is now well established in the progression of hepatocellular carcinoma (HCC) linked to Hepatitis C virus (HCV) infection. However, nothing is known about the potential interplay between Tregs and HCV. In this pilot study, we have investigated the ability of Tregs to hang HCV on and the subsequent...
Article
Full-text available
Colorectal cancer (CRC) remains the most cancer type related to chronic inflammation; however, the mechanisms that link inflammation to CRC development and progression are still poorly understood. Our study aimed to investigate one of the prominent inflammatory response in cancers, iNOS/NO system. In this regard, we evaluated the link between the i...
Article
Background: Immune response failure against hepatitis C virus (HCV) has been associated with an increased regulatory T cell (Treg) activity. After liver transplantation (LT), 80% of patients experience an accelerated progression of hepatitis C recurrence. The aim of this work was to assess the involvement of Tregs, T helper (Th) 1, 2 and 17 cells...
Article
Full-text available
Extra-cellular galectin-9 (gal-9) is an immuno-modulatory protein with predominant immunosuppressive effects. Inappropriate production of gal-9 has been reported in several human malignancies and viral diseases like nasopharyngeal, pancreatic and renal carcinomas, metastatic melanomas and chronic active viral hepatitis. Therefore therapeutic antibo...
Data
Capture of biotinylated galectins on surface-bound antibodies. The binding of Gal-Nab1, Gal-Nab2 and control mouse IgG1 antibodies to gal-9 was assessed by direct ELISA as described under “Materials & Methods” with the difference that biotinylated gal-9S (1 nM) was incubated during 1h at 37°C in antibody-coated wells in the presence or absence of l...
Data
Gal-9 surface staining on primary T cells. CD3+ purified T cells were stimulated (CD3/CD28 coated beads) or not and incubated with anti-MHC-I antibodies (as positive control), anti-gal-9 mAbs (9M1, Gal-Nab1 and Gal-Nab2; 5 μg/ml) or mouse irrelevant IgG1 for 30 min at 4°C. Cells were then washed in ice-cold PBS and incubated 30 min at 4°C with rat...
Article
Full-text available
Background: We analyzed telomere maintenance mechanisms (TMMs) in lymph node samples from HL patients treated with standard therapy. The TMMs correlated with clinical outcomes of patients. Materials and Methods: Lymph node biopsies obtained from 38 HL patients and 24 patients with lymphadenitis were included in this study. Seven HL cell lines were...
Conference Paper
Full-text available
Introduction Epithelial ovarian cancer (EOC) management is underlined by the high rate of peritoneal recurrence that may be related to microscopic peritoneal metastases ignored by conventional cytoreductive surgery. Intraperitoneal photodynamic therapy (PDT) could improve microscopic cytoreduction. In addition, immune response seems to be enhanced...
Poster
Full-text available
Objectives Epithelial ovarian cancer (EOC) management is underlined by the high rate of peritoneal recurrence that may be related to microscopic peritoneal metastases ignored by conventional cytoreductive surgery. Intraperitoneal photodynamic therapy (PDT) could improve microscopic cytoreduction. In addition, immune response seems to be enhanced af...
Presentation
Full-text available
To enable intraperitoneal photodynamic therapy (PDT) of peritoneal metastases, whichever their origin, is probably the most difficult challenge encountered by researchers involved in photodynamic applications. Indeed, intraperitoneal indications raise the issue of illumination delivery and its difficulty related to the peritoneal cavity’s complex a...
Article
Full-text available
Colitis associated cancer (CAC) is the colorectal cancer (CRC) subtype that is associated with bowel disease such as ulcerative colitis (UC).The data on role of NF-𝜅B signaling in development and progression of CAC were derived from preclinical studies, whereas data from human are rare.The aim of this work was to study the contribution of NF-𝜅B pat...
Conference Paper
Full-text available
Table of contents A1 Hope and despair in the current treatment of nasopharyngeal cancer IB Tan I1 NPC international incidence and risk factors Ellen T Chang I2 Familial nasopharyngeal carcinoma and the use of biomarkers Chien-Jen Chen, Wan-Lun Hsu, Yin-Chu Chien I3 Genetic susceptibility risk factors for sporadic and familial NPC: recent findings A...
Patent
The invention relates to an antibody which is directed against galectin‑9 and is an inhibitor of the suppressor activity of regulatory T lymphocytes, and also to the use of this antibody for the treatment of diseases associated with the suppressor activity of regulatory T lymphocytes, in particular the treatment of cancer.
Article
Full-text available
Galectin-9 (gal-9) is a multifunctional β-galactoside-binding lectin, frequently released in the extra-cellular medium, where it acts as a pleiotropic immune modulator. Despite its overall immunosuppressive effects, a recent study has reported a bimodal action of gal-9 on human resting blood T cells with apoptosis occurring in a majority of them, f...
Article
Full-text available
Background: Regulatory T cells (Treg) and tumor-exosomes are thought to play a role in preventing the rejection of malignant cells in patients bearing nasopharyngeal carcinoma (NPC). Methods: Treg recruitment by exosomes derived from NPC cell lines (C15/C17-Exo), exosomes isolated from NPC patients’ plasma (Patient-Exo), and CCL20 were tested in v...
Article
Full-text available
Virus infections are involved in chronic inflammation and, in some cases, cancer development. Although a viral infection activates the immune system’s response that eradicates the pathogen mainly through inflammatory mechanisms, it is now recognized that this inflammatory condition is also favorable to the development of tumors. Indeed, it is well...
Article
Epstein–Barr Virus (EBV) is present in the neoplastic cells of around 20–30% of patients with Hodgkin Lymphoma (HL). Although, an immunosuppressive environment is currently described in HL patients, little is known concerning the regulatory mechanism induced by EBV proteins expression in tumour cells. This study aimed to investigate an association...
Article
Full-text available
The TRANSPEG study was a prospective study to assess the efficacy of antiviral therapy in patients with a recurrent hepatitis C virus (HCV) after liver transplantation. The influence of regulatory T-cells (Tregs) on the response to antiviral therapy was analyzed. Patients were considered as a function of their sustained virological response (SVR) a...
Article
Full-text available
Hepatitis C virus (HCV) is an important causative agent of liver disease, but factors that determine the resolution or progression of infection are poorly understood. In this study, we suggested that existence of immunosuppressive mechanisms, supported by regulatory T cells and especially the regulatory T cell 1 subset (Tr1), may explain the impair...
Article
Full-text available
Inflammatory bowel diseases (IBDs) are chronic inflammatory diseases of the gastrointestinal tract, which are clinically present as 1 of the 2 disorders, Crohn's disease (CD) or ulcerative colitis (UC) (Rogler 2004 ). The immune dysregulation in the intestine plays a critical role in the pathogenesis of IBD, involving a wide range of molecules, inc...
Article
Inevitable hepatitis C virus recurrence after liver transplantation, a major barrier to survival of the transplanted liver may be promoted by immunosuppression and by CD4(+)CD25(+) regulatory T cells (Treg). Treg cells are essential for the induction and maintenance of immunologic self-tolerance as well as transplant tolerance. Moreover, we have pr...
Article
Full-text available
Liver transplantation is the treatment of end-stage liver diseases, including hepatitis C. Immunosuppression prevents graft rejection but seems to accelerate the recurrence of hepatitis C. Regulatory T cells (Tregs) may be beneficial in tolerance but deleterious in recurrent hepatitis C. We evaluated the effects of cyclosporine or tacrolimus, the p...
Article
Full-text available
CCL18 is both a constitutively expressed and an inducible chemokine, whose role in the inflammatory reaction is poorly known. The aim of this study was to evaluate whether CCL18 has the capacity to attract human T cells with a regulatory function (regulatory T cells [Treg]). Results from chemotaxis assays performed on different types of Treg showed...
Article
Epstein-Barr virus (EBV) is associated with several malignant diseases that can be distinguished by their patterns of viral latent gene expression. We developed here an original peptidic approach to favor the induction of a specific CD4 T-cell response against EBV latency II malignancies (Hodgkin's lymphoma, nasopharyngeal carcinoma, T/NK lymphoma)...
Article
Epstein-Barr virus (EBV) is associated with several malignant diseases that can be distinguished by their patterns of viral latent gene expression. We developed here an original peptidic approach to favor the induction of a specific CD4+ T-cell response against EBV latency II malignancies (Hodgkin's lymphoma, nasopharyngeal carcinoma, T/NK lymphoma...
Article
Full-text available
H-1 parvovirus (H-1 PV), a rodent autonomous oncolytic parvovirus, has emerged as a novel class of promising anticancer agents, because of its ability to selectively find and destroy malignant cells. However, to probe H-1 PV multimodal antitumor potential one of the major prerequisites is to decipher H-1 PV direct interplay with human immune system...
Article
Full-text available
Epstein-Barr virus (EBV) is a common human herpesvirus. Infection with EBV is associated with several human malignancies in which the virus expresses a set of latent proteins, among which is latent membrane protein 1 (LMP1). LMP1 is able to transform numerous cell types and is considered the main oncogenic protein of EBV. The mechanism of action is...
Article
Full-text available
The Epstein-Barr virus (EBV) is a common human herpes virus. Its infection is associated with several human malignancies where it expresses a set of latent proteins among which is the latent membrane protein LMP1. LMP1 is able to transform numerous cell types and is considered the main oncogenic protein of EBV. The mechanism of action is based on m...
Article
Full-text available
The aim of this study was to evaluate the nonchemotactic function of CCL18 on human dendritic cells (DCs). In different protocols of DC differentiation, CCL18 was highly produced, suggesting that it may constitute a mandatory mediator of the differentiation process. Differentiation of monocytes from healthy subjects in the presence of granulocyte-m...
Article
The purpose of this study was to assess the direct effect of CCL18, a chemokine elevated in allergic diseases and induced by Th2 cytokines, on the polarization of human CD4(+) T cells. Purified human T cells from healthy subjects were pretreated or not with CCL18, and evaluated for cytokine production. CCL18-pretreated memory but not naive CD4(+) T...
Article
Full-text available
Inevitable hepatitis C virus (HCV) recurrence after liver transplantation is a major barrier to the survival of a transplanted liver. It may be promoted by immunosuppression and the emergence of CD4+CD25+ regulatory T cells (Treg). Treg cells can mediate the induction and maintenance of immunological self-tolerance as well as transplant tolerance....
Article
Immune response failure during HCV infection has been associated with the activity of regulatory T cells. Hepatitis C-related cirrhosis is the main reason for liver transplantation. However, 80% of transplanted patients present an accelerated recurrence of the disease. This study assessed the involvement of regulatory T-cell subsets (CD4+CD25+ cell...
Article
Hepatitis C related cirrhosis is one of the main cause of liver transplantation. However, 80% of transplanted patients present an accelerate recurrence of the disease, leading in 20% case to a new cirrhosis 5 years later. Immune response failure during HCV primo-infection has been associated with regulatory T cells activity. For the first time, it...
Article
Alternative, non-invasive techniques are necessary to monitor the progression of liver disease during chronic hepatitis C. Firstly, because serum is the most accessible material for studies using qPCR in microplates, gene transcription was compared in 219 selected genes involved in the pathogenesis of hepatitis C virus (HCV) infection between sera,...