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Olivier Laprévote

Olivier Laprévote
University of Paris, CPSC

Pharm D PhD HDR
Professor of Toxicology Head of the Mass Spectrometry and Metabolomics platform

About

308
Publications
46,394
Reads
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8,405
Citations
Citations since 2017
42 Research Items
3056 Citations
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
Additional affiliations
January 2014 - present
Université Paris Descartes
Position
  • Director of the research unit CNRS UMR8638
October 2008 - December 2013
Paris Descartes, CPSC
Position
  • Principal Investigator
October 2008 - present
Assistance Publique – Hôpitaux de Paris
Position
  • Director of the Biological Toxicology Department
Description
  • Hôpital Lariboisière 2, rue Ambroise Paré 75 010 PARIS
Education
September 1986 - December 1989
Université Paris-Sud
Field of study
  • Pharmacognosy, Analytical Chemistry, Pharmaceutical Sciences
September 1985 - June 1986
Université Paris-Sud 11
Field of study
  • Organic Chemistry
September 1980 - November 1986
Université Paris-Sud 11
Field of study
  • Pharmacy

Publications

Publications (308)
Article
A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous determination of tryptophan (Trp) and ten metabolites of kynurenine pathway, including kynurenine (Kyn), 3-hydroxy-kynurenine (3-HK), kynurenic acid (KA), xanthurenic acid (XA), 3-Hydroxy-anthranilic acid (3-HANA),...
Article
In the central nervous system, lipids represent approximately 70% of myelin dry weight and play a key role in axon insulation and action potential conduction velocity. Lipids may thus represent sensitive markers of myelin status in physiological and pathological contexts. In this study, a comprehensive lipidomic analysis by ultra-high-performance l...
Article
Three new very long chain polyunsaturated fatty acids (VLC PUFA) belonging to the omega-3 family have been identified in meibum samples collected by Schirmer strips. These VLC PUFA, namely FA (32:3), FA (34:3) and FA (36:3), were detected in O-acyl-ω-hydroxy fatty acids using a molecular network approach, and as free fatty acids. Identification was...
Article
Full-text available
In the central nervous system, the process of myelination involves oligodendrocytes that wrap myelin around axons. Myelin sheaths are mainly composed of lipids and ensure efficient conduction of action potentials. Oligodendrocyte differentiation is an essential preliminary step to myelination which, in turn, is a key event of neurodevelopment. Bisp...
Article
Full-text available
Three-component cascade reactions of (E)-3-arylidene-1-methyl-pyrrolidine-2,5-diones, L-valine and various isatin derivatives are described. A series of 17 spiropyrrolidine derivatives with wide structural complexity and diversity have been thus obtained in moderate to excellent yields under mild reaction conditions. The structure and stereochemist...
Article
The in-depth knowledge of lipid biological functions needs a comprehensive structural annotation including a method to locate fatty acid unsaturations, which remains a thorny problem. For this purpose, we have associated Grubbs’ cross-metathesis reaction and liquid chromatography hyphenated to tandem mass spectrometry to locate double bond position...
Article
Rationale: Accurate and reliable measurements are mandatory in the field of environmental monitoring. Matrix effects are often depicted as the Achilles heel of LC-MS analysis since they may be prejudicial for analytical performances such as detection capability and accuracy if not documented nor compensated. Here a methodological work for the eval...
Article
Natural and synthetic steroid hormones are chronically released into aquatic spheres. Whereas knowledge on their combined mode of action and the cocktail effect are needed, only few multi-class methods address the challenge of their trace quantification in surface waters. The current study describes a sensitive multi-residue analytical strategy aim...
Article
In the central and peripheral nervous systems, myelin is essential for efficient conduction of action potentials. During development, oligodendrocytes and Schwann cells differentiate and ensure axon myelination, and disruption of these processes can contribute to neurodevelopmental disorders. In adults, demyelination can lead to important disabilit...
Preprint
The in-depth knowledge of lipid biological functions calls for a comprehensive lipid structure annotation that implies implementing a method to locate fatty acids unsaturations. To address this challenge we have associated Grubbs' cross metathesis reaction and liquid chromatography hyphenated to tandem mass spectrometry. The pretreatment of lipids...
Article
Detergent chemicals, widely used in household products, in pharmaceutical, medical, cosmetic and industrial fields, have been linked to side effects and involved in several eye diseases. On the ocular surface, detergents can interfere with the corneal epithelium, the most superficial layer of the cornea, representing a line of defence against exter...
Article
Full-text available
Pretherapeutic screening for dihydropyrimidine dehydrogenase (DPD) deficiency is recommended or required prior to the administration of fluoropyrimidine-based chemotherapy. However, the best strategy to identify DPD-deficient patients remains elusive. Among a nationwide cohort of 5886 phenotyped patients with cancer who were screened for DPD defici...
Article
Full-text available
Annotation of lipids in untargeted lipidomic analysis remains challenging and a systematic approach needs to be developed to organize important datasets with the help of bioinformatic tools. For this purpose, we combined tandem mass spectrometry-based molecular networking with retention time (t R) prediction to annotate phospholipid and sphingolipi...
Article
Dry eye disease (DED) is a multifactorial chronic inflammatory disease of the ocular surface characterized by tear film instability, hyperosmolarity, cell damage and inflammation. Hyperosmolarity is strongly established as the core mechanism of the DED. Benzalkonium chloride (BAK) - a quaternary ammonium salt commonly used in eye drops for its micr...
Article
Full-text available
This paper reports the isolation and structural characterization of four new ent-kaurane derivatives from the Lamiaceae plant Sideritis hyssopifolia. Planar structures and relative configurations were determined using both mass spectrometry and nuclear magnetic resonance (1D and 2D). Absolute configurations were determined by comparing experimental...
Article
Full-text available
The prevalence of cognitive decline is increasing as the ageing population is considerably growing. Restricting this age-associated process has become a challenging public health issue. The age-related increase in oxidative stress plays a major role in cognitive decline, because of its harmful effect on functional plasticity of the brain, such as l...
Article
Full-text available
Background: Phthalates are environmental contaminants commonly used as plasticizers in polyvinyl chloride (PVC) products. Recently, exposure to phthalates has been associated with preterm birth, low birth weight, and pregnancy loss. There is limited information about the possible mechanisms linking maternal phthalate exposure and placental develop...
Article
Purpose To better understand how quantitative sensory testing could help the clinician in the management of oxaliplatin-induced peripheral neuropathy in terms of earlier and more reliable detection, we conducted a two-year prospective study. Methods Thermal sensory assessment, tactile sensory assessment, neuropathic pain assessment and adverse eve...
Article
A series of new 1,2,3-triazole linked coumarinopyrazole conjugates 4a-e and 5a-e have been synthesized via the Copper(I)-catalysed Alkyne-Azide Cycloaddition (CuAAC). Going through the reaction of compound 2 with the 3-propargyl bromide gave a mixture of propargylated regioisomers 3 + 3' used as a dipolarophile to access to triazoles 4a-e and 5a-e....
Article
Full-text available
Re-investigation of the chemical composition of the annual plant Mitracarpus scaber Zucc. led to the identification of clarinoside, a new pentalogin derivative containing a rare quinovose moiety, and the known compound harounoside. While the planar structure was fully determined using tandem mass spectrometry (MS) and quantum mechanics (QM) calcula...
Preprint
Re-investigation of the chemical composition of the annual plant Mitracarpus scaber Zucc. led to the identification of clarinoside, a new pentalogin derivative containing a rare quinovose moiety, and the known compound harounoside. While the planar structure was fully determined using tandem MS and quantum mechanics calculations (QM), the tridimens...
Preprint
Re-investigation of the chemical composition of the annual plant Mitracarpus scaber Zucc. led to the identification of a new pentalogin derivative containing a rare quinovose moiety. While the planar structure has been fully determined using tandem MS and quantum mechanics calculations (QM), the tridimensional structure was unraveled after isolation...
Article
Full-text available
During pregnancy, exposure to environmental contaminants can lead to adverse effects on fetal growth and development, especially by targeting the placenta. Di(2-ethylhexyl)phthalate (DEHP), the most abundant chemical used in plastic materials, is known to induce toxicity on animals reproductive system and is suspected to give rise to similar effect...
Article
Full-text available
Resveratrol (RES), a polyphenol found in natural foods, displays anti-oxidant, anti-inflammatory and anti-proliferative properties potentially beneficial in cancers, in particular in the prevention of tumor growth. However, the rapid metabolism of resveratrol strongly limits its bioavailability. The molecular mechanisms sustaining the potential bio...
Article
Full-text available
The Aryl hydrocarbon Receptor(AhR) is among the most important receptors which bind pollutants; however it also regulates signaling pathways independently of such exposure. We previously demonstrated that AhR is expressed during development of the central nervous system(CNS) and that its deletion leads to the occurrence of a congenital nystagmus. O...
Article
Degenerative diseases are characterized by both cell death and inflammation, which involve different pathways such as apoptosis and pyroptosis. Oxysterols, oxidized derivatives of cholesterol, are known to act as key actors in degenerative disorders such as skin photoaging. We hypothesize that oxysterols could be implicated in either apoptosis or p...
Article
Introduction Addiction to psychoactive substances (PAS) can lead to cardiovascular complications. Cardiotoxicity of drugs is known but it is rarely documented by toxicology. Objectives We conducted a prevalence study on PAS use among patients with cardiac symptoms, with an analysis of diagnosis and a description of PAS user's characteristics. Aim...
Article
A series of S-mono and S,O-bis-1,2,3-triazole linked 1,5-benzodiazepines (BZD) conjugates 6a-j and 7a-j were synthesized employing “click chemistry” under microwave irradiation. The synthesized derivatives have been evaluated for their cytotoxic (MCF-7, HeLa, A549 cell lines), anti-tyrosinase and anti-cholinesterase activities. These molecules exhi...
Article
Scope: Trans-resveratrol is widely studied for its potentially beneficial effects on numerous disorders. It is rapidly metabolized and its metabolites can exhibit biological activity. The present study aimed to investigate whether acute or sustained trans-resveratrol administration impacted on the distribution of trans-resveratrol and its metaboli...
Article
The supply of recreational drugs has changed in the early 2000s and users increasingly buy cathinones over the Internet. There is a potential for significant toxicity associated with their use. Cathinone named “NRG-3” seems to be the most popular in Parisian CEIP data: the adverse events related to use of NRG-3 from 2011 to 2015 are reported. We ai...
Article
Objectif Vérifier que les résultats rendus dans un contexte d’urgence (CL–UV/BD–SM) sont bien corrélés aux résultats obtenus par la technique de référence (CL–SM/SM), et cela pour trois benzodiazépines courantes : le diazépam, le nordiazépam et l’oxazépam. Description Les benzodiazépines sont les psychotropes les plus prescrits dans la prise en ch...
Article
Objectif Présenter un tableau d’intoxication aiguë aux opiacés chez un enfant soupçonné d’avoir été exposé à de l’héroïne dans son environnement familial. Description Un enfant de 8 ans est admis en réanimation dans un état de coma profond avec atteinte neurologique sévère. Après que l’enfant se soit endormi vers 00h30, son père prévient le SAMU à...
Article
Contexte Le criblage toxicologique s’appuie sur un ensemble de méthodes analytiques hétérogènes visant à apporter au clinicien des informations rapides et fiables pour la prise en charge des patients intoxiqués. À ce jour, malgré les discussions des experts autour d’un consensus [1], la stratégie analytique du criblage reste avant tout conditionnée...
Article
Objectif Comparer les résultats obtenus en toxicologie hospitalière entre deux approches de criblage systématique par CG–SM (urine)/CL–UV/BD–SM (plasma) et CL–SMn. Description La nécessité d’élaborer des stratégies génériques pour cribler et/ou quantifier de nombreux composés dans les laboratoires de toxicologie demeure un défi. Les matrices les p...
Article
Skin photoaging due to UV irradiation is a degenerative process that appears more and more as a growing concern. Lipids, including oxysterols, are involved in degenerative processes; as skin cells contain various lipids, the aim of our study was to evaluate first, changes in keratinocyte lipid levels induced by UV exposure and second, cellular effe...
Article
Age-related macular degeneration (AMD) is the most common cause of severe vision loss worldwide. Amyloid β involvement in degenerative diseases such as AMD is well known and its toxicity has been related to P2X7 receptor-pannexin-1. Recently, oxysterols (oxidized derivatives of cholesterol) have been implicated in AMD pathogenesis. The aim of our s...
Article
Nude mice carry a spontaneous mutation affecting the gene Foxn1 mainly expressed in the epidermis. This gene is involved in several skin functions, especially in the proliferation and the differentiation of keratinocytes which are key cells of epithelial barrier. The skin, a protective barrier for the body, is essentially composed of lipids. Taking...
Article
Full-text available
Alzheimer's disease (AD) is characterized by both amyloid and Tau pathologies. The amyloid component and altered cholesterol metabolism are closely linked, but the relationship between Tau pathology and cholesterol is currently unclear. Brain cholesterol is synthesized in situ and cannot cross the blood–brain barrier: to be exported from the centra...
Article
Full-text available
Abnormalities in neuronal cholesterol homeostasis have been suspected or observed in several neurodegenerative disorders including Alzheimer's disease, Parkinson's disease and Huntington's disease. However, it has not been demonstrated whether an increased abundance of cholesterol in neurons in vivo contributes to neurodegeneration. To address this...
Article
Objectifs En toxicologie hospitalière, le but est d’identifier et de quantifier des médicaments/drogues inconnus responsables d’intoxications. La fiabilité des résultats de laboratoire ne dépend pas uniquement d’une technique d’analyse robuste, une préparation de l’échantillon dans les règles de l’art doit précéder la phase analytique. Cette phase...
Article
This study reports an energy-resolved mass spectrometric (ERMS) strategy for the characterization of position isomers derived from the reaction of hydroxyl radicals (●OH) with diphenhydramine (DPH) that are usually hard to differentiate by other methods. The isomer analogues formed by ●OH attack on the side chain of DPH are identified with the help...
Conference Paper
Workshops are an important part of the IFPA annual meeting as they allow for discussion of specialized topics. At IFPA meeting 2014 there were six themed workshops, five of which are summarized in this report. These workshops related to various aspects of placental biology but collectively covered areas of animal models, xenobiotics, pathological b...
Article
Mass spectrometry imaging has become a popular tool for probing the chemical complexity of biological surfaces. This led to the development of a wide range of instrumentation and preparation protocols. It is thus desirable to evaluate and compare the data output from different methodologies and mass spectrometers. Here, we present an approach for t...
Article
The development of ‘omics’ sciences offers new opportunities for the study of neurodegenerative diseases but increases at the same time the sample demand on brain banks that collect and store valuable human post-mortem tissue. Our study aims to evaluate in lipidomics the potential of formalin-fixed tissue compared with the cryopreservation method,...
Article
While acting upon chromatin compaction, histone post-translational modifications (PTMs) are involved in modulating gene expression through histone-DNA affinity and protein-protein interactions. These dynamic and environment-sensitive modifications are constitutive of the histone code that reflects the transient transcriptional state of the chromati...
Article
A rapid, sensitive and specific method using liquid chromatography coupled to tandem mass spectrometry was developed for the simultaneous quantification of hydroxychloroquine (HCQ) and its three major metabolites in human whole blood. The assay, using a sample volume of 100μL, was linear in a dynamic 25-2000ng/mL range (R(2)>0.99) for all four comp...
Article
Full-text available
Background Methadone maintenance treatment is the most widely prescribed treatment for opiate dependence with proven benefits for patients. In naïve users or in case of recreational misuse, methadone can be a source of potentially lethal intoxications, resulting in fatal overdoses. A few cases of infantile intoxications have been described in the l...
Article
Full-text available
Cytochromes P450 (CYPs) play critical roles in oxidative metabolism of many endogenous and exogenous compounds. Protein expression levels of CYPs in liver provide relevant information for a better understanding of the importance of CYPs in pharmacology and toxicology. This work aimed at establishing a simple method to quantify six CYPs (CYP3A4, CYP...
Article
The grey mouse lemur (Microcebus murinus) is a non-human primate used to study the ageing process. Resveratrol is a polyphenol that may increase lifespan by delaying age-associated pathologies. However, no information about resveratrol absorption and metabolism is available for this primate. Resveratrol and its metabolites were qualitatively and qu...
Article
The senile plaque is a hallmark lesion of Alzheimer disease (AD). We compared, without a priori, the lipidome of the senile plaques and of the adjacent plaque-free neuropil. The analysis by liquid chromatography coupled with electrospray ionization mass spectrometry revealed that laser microdissected senile plaques were enriched in saturated cerami...