Oliver Tusevski

Saints Cyril and Methodius University of Skopje · Faculty of Natural Sciences and Mathematics

Hypericum perforatum transformed shoot lines (TSL) regenerated from corresponding hairy roots and non-transformed shoots (NTS) were comparatively evaluated for their phenolic com- pound contents and in vitro inhibitory capacity against target enzymes (monoamine oxidase-A, cholinesterases, tyrosinase, α-amylase, α-glucosidase, lipase, and cholestero...

This study has been made to develop an efficient protocol for micropropagation of Cannabis sativa L. cultivar Bubba Kush x OG Kush allowing us to examine cannabinoid production in various in vitro cultures. The role of plant growth regulators has been particularly studied. Three in vitro culture lines with different morphological characteristics we...

Hairy root (HR) cultures of Hypericum perforatum L. have shown promise in controlling hyperglycemia, regulating blood lipid and enzyme profiles, and improving metabolic function in vivo. These benefits are mainly attributed to the phenolic acids, flavonoids, and xanthones present in HR extracts. However, the specific mechanism underlying these effe...

Hypericum perforatum L. hairy root clones cultured under dark (HR1) and photoperiod (HR2) conditions were comparatively assessed for the contents of phenolic compounds and their inhibitory potential against enzymes related to depression, neurodegeneration, diabetes and hyperlipidemia. In silico docking methods were performed to examine the molecula...

Citation: Tusevski, O.; Todorovska, M.; Todorovska, I.; Petreska Stanoeva, J.; Gadzovska Simic, S. Abstract: Fifteen transgenic shoot clones (TS A-TS O) regenerated from Hypericum perforatum hairy roots were evaluated for growth characteristics, phenylpropanoid, and naphthodianthrone production , as well as non-enzymatic and enzymatic antioxidant s...

Fifteen transgenic shoot clones (TS A-TS O) regenerated from Hypericum perforatum hairy roots were evaluated for growth characteristics, phenylpropanoid, and naphthodianthrone production, as well as non-enzymatic and enzymatic antioxidant status. Transgenic shoots displayed higher biomass production and non-altered phenotype compared to control sho...

Objective: The main objective of this study was to determine the influence of various cytokinins on the production of cannabinoids and total phenolics, as well on antioxidant capacity in callus cultures of cannabis (Cannabis sativa L.). Methods: Calli were obtained on MS/B 5 medium [1,2] with various concentrations (0.5, 1.0, 1.5 and 2.0 mg·L-1) of...

The aim of this study was to evaluate the non-enzymatic and enzymatic antioxidant response of fifteen Hypericum perforatum L. dark-grown (HR1 A-HR1 O) and photoperiod-exposed (HR2 A-HR2 O) hairy root clones. Dark-grown HR1 clones were characterized with high biomass accumulation and up-regulated phenylpropanoid metabolism through phenylalanine ammo...

Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depre...

Ethnopharmacological relevance Hypericum perforatum L., commonly known as St. John's Wort (SJW), represents one of the best-known and most thoroughly researched medicinal plant species. The ethnobotanical usage and bioactivities related to H. perforatum include treatment of skin diseases, wounds and burns, gastrointestinal problems, urogenital dise...

Hypericum perforatum hairy root (HR) clones obtained by Agrobacterium rhizogenes A4-mediated transformation were analyzed for in vitro inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as key enzymes related to neurodegeneration. In silico molecular modeling was performed using AutoDock 4.2 software to predict...

The aim of this study was to investigate the effect of salicylic acid (SA: 50, 100 and 250 μМ) on the growth, production of total phenolics (TP), flavonoids (TF) and xanthones (TX), as well antioxidant capacity (CUPRAC assay) in hairy root (HR) cultures of Hypercum perforatum during the period of post-elicitation (day 1, 4, 7, 14, 21 and 28). The...

Apart from currently available therapeutics for the treatment of diabetes mellitus, much attention has been paid to discover phytochemicals from natural resources, mainly due to their low side-effects. Hypericum perforatum hairy root (HR) transformed with Agrobacterium rhizogenes A4 represent prospective experimental system enriched in xanthones, k...

Cannabis sativa L. (Cannabaceae) is an important medicinal plant well known for its pharmacological and therapeutic activity. High commercial interest, limited natural resources and variable phytochemical composition of wild growing C. sativa plants are the main reasons for cultivation of this plant in controlled conditions. A quite challenging tas...

In the present study, methanolic extracts from 30 plant species collected on Galichitsa Mt. were evaluated for their antioxidant properties using CUPRAC (CUPric Reducing Antioxidant Capacity) assay and total phenolic (TP) content according to the Folin-Ciocalteau method. Antioxidant properties and total phenolic content differed significantly among...

Thirty-two different solvent mixtures containing methanol and acid (acetic, formic, and hydrochloric); methanol, water, and acid; and pure methanol were tested for their efficiency for extraction of phenolic compounds from aronia ( Aronia melanocarpa) belonging to the groups of anthocyanins, flavonols, and hydroxycinnamic acid derivatives. Thirteen...

Altogether, 14 basidiomycetes (12BAD, 95PCH, 9WCOC, 5PSA, 96BCI, 331SHIBD, 4MSC, 74HFA, 220MPS, 115PFLA, 111 ICO C, 16LED, 6TSU, and 61LYP) were grown on solid and in liquid media using hairy roots of genetically modified Hypericum perforatum (L.) as the only source of carbon and nitrogen. After the first screening by GC‐MS/MS‐O, two fungi (115PFLA...

The aim of this study was to evaluate phenolic compounds composition and antioxidant activity in roots (RO), non-flowering shoots (NFS) and flowering shoots (FS) of Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia. The analyses of total phenolic compounds included quantification of phenolics, flavonoids, catechin d...

Three transgenic shoot clones (TS B, TS F and TS H) of Hypericum perforatum L. were evaluated for phenolic compounds composition and in vitro antidiabetic activity. The TS clones were spontaneously regenerated from the corresponding hairy root cultures induced by Agrobacterium rhizogenes A4-mediated transformation. Phenolic compounds in TS clones a...

Hypericum perforatum hairy root clones (HR A–HR O) transformed with Agrobacterium rhizogenes A4 were evaluated for growth characteristics, phenolic compounds production, antioxidant/radical scavenging activity, antioxidant enzymes, and oxidative stress markers. The screening of growth characteristics revealed that HR clones were characterized with...

The aim of this study was to evaluate phenolic compounds composition and antioxidant activity in roots (RO), non-flowering shoots (NFS) and flowering shoots (FS) of Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia. The analyses of total phenolic compounds included quantification of phenolics, flavonoids, catechin d...

The aim of this study was to provide comprehensive understanding of phenolic compounds composition in roots (RO), non-flower shoots (NFS) and flower shoots (FS) of Hypericum perforatum wild-growing plants from the Republic of Macedonia. Identification of phenolic compounds in plant methanolic extracts was performed by HPLC-DAD/ESI-MS analysis. Chlo...

The aim of this study was to evaluate the phytochemical composition, antimicrobial activity and enzyme inhibitory properties of roots (RO), non-flower shoots (NFS) and flower shoots (FS) of Hypericum perforatum L. originating from the Republic of Macedonia. The identification and quantification of phenolic compounds in plant material were performed...

The aim of this study was to evaluate the contents of total phenolics (TP) and flavonoids (TF), as well the antioxidant activity (DPPH) in methanolic extracts of leaf, stem, flower and aerial part from fourteen Hypericum taxa: H. perforatum var. perforatum L., H. perforatum var. angustifolium DC., H. perforatum var. microphyllum DC., H. barbatum va...

This is the first report for the effect of cytokinin N6-benzyladenine (BA) on biomass production and morphological characteristics of Hypericum barbatum Jacq. shoot cultures. Shoot cultures of H. barbatum were established from epicotyls isolated from in vitro grown seedlings and cultivated on MS/B5 medium supplemented with BA (0.1, 0.2, 0.5 and 1.0...

Methanolic extracts from Hypericum perforatum L. hairy root clones (HR B, HR F and HR H) and non-transformed roots (NTR) were evaluated for total phenolic (TP) and flavonoid (TF) contents and antioxidant activities using ABTS and CUPRAC assays. The in vitro antidiabetic effect of plant extracts was investigated through inhibition of α-amylase (α-AM...

The aim of this study was to provide comprehensive understanding of phenolic compounds distribution in roots (RO), non-flower shoots (NFS) and flower shoots (FS) of Hypericum perforatum L. wild-growing plants. Chromatographic analysis of plant methanolic extracts revealed the presence of phenolic acids, flavan-3-ols, flavonol glucosides and aglycon...

The up-regulation of antioxidant enzymes is a well-known defense mechanism for neutralization of reactive oxygen species (ROS) in plant tissues upon biotic stress. The objective of this work was to investigate the correlation between antioxidant enzyme activity and oxidative stress marker contents in Hypericum perforatum L. transgenic shoots (TS)....

Hypericum perforatum transgenic shoots (TS) were spontaneously regenerated from the corresponding hairy roots induced by Agrobacterium rhizogenes A4-mediated transformation. The aim of this study was to evaluate the phenolic compounds composition and antimicrobial activity of H. perforatum transgenic shoot extracts. The HPLC/DAD/ESI-MSn analysis of...

Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis o...

Macrofungi have long been valued as nutritional food and an important part of the traditional medicine by different cultures worldwide. They contain large number of secondary metabolites which have antioxidant properties. Phenolic compounds are widely distributed secondary metabolites in mushrooms, that are correlated with their antioxidant activit...

The genus Hypericum belongs to Hypericaceae, a family composed by species with important medicinal properties. In Republic of Macedonia, the genus Hypericum includes 15 species, 3 subspecies,10 varieties and one endemic species H. dimoniei. This study reports for the first time the production of phenolic compounds and antioxidant activity in differ...

Hypericum perforatum callus cultures exhibited qualitatively and quantitatively different phenolic profile compared to wild-growing plants (WGP). This study revealed the presence of phenolic acids, flavonoid glycosides and aglycones, flavan-3-ols and naphtodianthrones in callus and WGP extracts. Phloroglucinols were detected only in WGP, while xant...

Hypericum perforatum cell suspensions were evaluated for their growth, phenylpropanoid and naphtodianthrone productions, and antioxidant activity after treatments with polysaccharide elicitors chitin (CHI), pectin (PEC) and dextran (DEX). Polysaccharide elicitation of cell cultures showed a considerable improving effect on phenylpropanoid productio...

Hypericum perforatum L. cell suspensions were evaluated for their growth, antioxidant activity, phenolic compound productions and xanthone profile after elicitation with Agrobacterium tumefaciens and Agrobacterium rhizogenes. Secondary metabolite production in H. perforatum elicited cells was enhanced without loss of biomass. A. tumefaciens elicite...

Hypericum perforatum cell suspensions were evaluated for their growth, phenylpropanoid and naphtodianthrone productions, and antioxidant activity after treatments with fungal elicitors Fusarium oxysporum, Phoma exigua and Botrytis cinerea. Elicited cells displayed a reduced biomass production, a rapid stimulation of secondary metabolites production...

The aim of this paper is to show that infection of tobacco (Nicotiana tabacum) with a fresh culture of Agrobacterium rhizogenes A4, under in vitro conditions, lead to genetic transformation. The interaction between the t-DNA sequence of Ri plasmid of Agrobacterium rhizogenes A4 and recipient cells of Nicotiana tabacum seed type Prilep variety P 12-...

The effects of polysaccharide elicitors such as chitin, pectin, and dextran on the production of phenylpropanoids (phenolics and flavonoids) and naphtodianthrones (hypericin and pseudohypericin) in Hypericum perforatum shoot cultures were studied. Nonenzymatic antioxidant properties (NEAOP) and peroxidase (POD) activity were also observed in shoot...

Investigations have been made to study the production of phenolic compounds (total phenolics, flavonoids and phenylpropanoids) and total antioxidant capacity in 27 Macedonian traditional medicinal plants to improve its potential as a source of natural antioxidants. Antioxidant potential of plant extracts was analyzed by five different assays: cupri...

Considering the global interest for the natural sources of antioxidants, macrofungi have become attractive for research activities. The aim of this study was to investigate the antioxidant properties of three Boletus species: B. appendiculatus, B. satanas and B. lupinus. Specimens were collected from Macedonian oak forest on Galichica Mountain in a...

Regeneration of transgenic shoots was achieved from Hypericum perforatum L. hairy roots on hormone-free MS/B5 medium for a period of 4 weeks under a photoperiod of 16-h light. A control experiment was set up with root segments obtained from in vitro grown seedlings. Investigations have been made to study the production of phenolic compounds in non...

St. John’s wort (Hypericum perforatum L.) has received considerable interest in recent years due to the increased market demand for Hyperici herba crude material as a source of bioactive pharmaceuticals. Hypericum extracts contain a complex mixture of bioactive metabolites, mainly naphthodianthrones (hypericin and pseudohypericin), phloroglucinols...

the Republic of Macedonia Secondary metabolites have a specific role in the organic matter and nutrient cycling in forest ecosystems, especially in the process of litter decomposition. Decomposition of Common beech (Fagus sylvatica L.) leaf, branch, and acorn litter was followed by litter-bag experiments in the Common beech ecosystem Calamintho gra...

The presence of phenolic compounds in the milk and cheese is a result of their transfer from plant to milk and can affect their antioxidant activity. The objective of this work is to make a survey of the prevalence of the total phenolic compounds (TPC) in certain stages during cheese ripening as well as to determinate their final concentration in m...

Hypericum perforatum L. is a medicinal plant considered as an important natural source of secondary metabolites with a wide range of pharmacological attributes. Hairy roots (HR) were induced from root segments of in vitro grown seedlings from H. perforatum after cocultivation with Agrobacterium rhizogenes A4. Investigations have been made to study...

Hypericum perforatum L. is a common perennial plant with a reputed medicinal value. Investigations have been made to develop an efficient protocol for the identification and quantification of secondary metabolites in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after...

Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was con...

We investigated the production of phenylpropanoids (phenolic compounds, flavanols, flavonols and anthocya-nins) and naphtodianthrones (hypericins) in elicited Hypericum perforatum L. cell suspensions. To determine whether secondary metabolite production could be enhanced, Hypericum cell suspensions were exposed to mycelia extract from the fungus As...

The main goal of the research was to summarize naphtodianthrone (hypericin and pseudohypericin) production in in vitro cell suspensions of H. perforatum L. A reversedphase high performance liquid chromatography (HPLC) method with fluorescence detection was used. Identification of the compounds was confirmed by mass spectrometry (ESI-MS) with electr...

Phenylpropanoid production in Hypericum perforatum L. cell suspensions in response to elicitation by Agrobacterium rhizogenes A4 co-cultivation has been studied. A reversedphase high performance liquid chromatography (HPLC-DAD) method with diode array detection was used. To determine whether secondary metabolite production could be enhanced in cell...

Recently, research activities in plant secondary metabolites are focused on their properties as a new source of natural antioxidants. In this study, nonenzymatic antioxidant acitvity (NEAA) of 20 Macedonian medicinal plants was determined using -carotene/linoleic acid oxidation method. Plant species were collected on the Jablanica Mt. at the altitu...