Olha Yaroshko

• Doctor of Philosophy
• assistant at Institute of Cell Biology and Genetic Engineering

Many species of Orchidaceae (Juss.) family have vulnerable or endangered status in Eastern and Western Europe. Among the main factors which influence on distribu on of orchids are ecological, biological and anthropogenic. Usually, the ways of orchid conserva on include research connected with taxonomy, geographic distribu on, impact evalua on of ec...

Human interferon alpha (huINFα2b) plays a crucial role in maintaining human immunity. It has antiviral properties and is used to treat a number of human diseases. The aim of this work was to obtain transgenic organs of Amaranthus caudatus plants expressing the infα2b gene, which can be used as future models to study the functional activity of inter...

P.1630 Direct regeneration of Drosera rotundifolia L. in vitro conditions Yaroshko Olha1 1 Genetic engineering Department, Institute of Cell biology and Genetic engineering NAS, UA. Drosera rotundifolia L. belongs to the Droseraceae family. Drosera rotundifolia is widespread species throughout the territory Holarctic (territory of America and Europ...

The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas, allows for the modification of physiological, morphological, and other characteristics in a wide range of crops to mitigate the negative effects of stress caused by anthropogenic climate change or biotic stresses. Importantly, these tools have the potential to impr...

The production of transgenic petunia plants with heterologous ZRNase II gene and the evaluation of resistance to Tobacco mosaic virus in the resultant plants was the aim of our research. We have generated transgenic petunia plants with ZRNase gene via Agrobacterium-mediated transformation. The presence of ZRNase II gene was confirmed by PCR-analysi...

Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the effect of different concentrations of zeatin and i...

The cultivated tomato (Solanum lycopersicum) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analysed the effect of different concentrations of zeatin and...

Ribonucleases (RNases) are supposed to be engaged to antiviral response in plants, so heterologous RNase gene expression can be a tool for production of cultivars with multiple resistance to viruses and viroids. Nicotiana benthamiana plants are susceptible to the large number of diverse plant viruses that can successfully infect it. The species is...

The aim of our investigation was to validate the expression of the heterologous HuINFalpha-2b gene in transgenic tomato lines

Local cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim. The purpose of the work was to identify the optimal conditions for the transient expression of reporter genes in Amaranth...

Despite the fact that in the modern world more than a thousand edible plants are used for food, only 3 staple cereal crops are grown worldwide: wheat, rice, and maize. Growing a limited number of crops often causes many problems: ranging from the loss of biodiversity, due to the constant cultivation of the same monocultures in the same areas, to th...

Amaranth is the C4-plant which used in various industries and is a promising object for genetic transformation. After «floral-dip» transformation of Helios and Karmin cultivars of Amaranthus caudatus L. using Agrobacterium rhizogenes A4 strain and A. tumefaciens GV3101 strain, transformed plants of the first generation were obtained. Gene vectors p...

The aim of the work was to establish the effective culture medium for the regeneration of Physalis peruviana for further micropropagation and obtaining of adult plants from regenerants in vitro conditions. After conducting series of experiments, effective culture media for the regeneration of P. peruviana was established. The most effective media f...

After “floral-dip” transformation of Amaranth plants with Agrobacterium tumefaciens strain GV3101 carrying pCBV19 gene vector that contained bar and gus genes, transgenic seeds were obtained. The functioning of the tran+sferred genes in Amaranthus tissues was confirmed with herbicide selection (PPT herbicide – phospinotricin) and gus gene activity....

The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum infiltration with Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties o...

Aim. To investigate the antibacterial properties of the fl owers of melliferous plants on the cultures isolated from honeycombs affected by foulbrood. Methods. Microbiological, cultural-morphological, biochemical, electron- microscopic, statistical. Results. Antibacterial effect on the Melissococcus pluton 8.1 strain was demonstrated by the fl ower...

Aim. To investigate the microbiota of honeycombs with affected bee brood. Methods. Visual, immunochro- matographic, cultural-morphological, biochemical, electron-microscopic methods were used to isolate and previously identify a number of microorganisms. Results. 10 samples of honeycombs with sealed brood were studied. The following agents of bee d...