Olga ZiminaInstitute of Molecular Biology and Genetics NAS of Ukraine · Department of Molecular Genetics
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Aim. Creation of genetic constructions to study the effects of various regulatory elements, namely promoters, on the expression of GFP reporter protein. Methods. For creation genetic constructs, the method of molecular cloning Golden Gate was used, which allows the rapid creation of genetic vectors using IIS type restriction enzymes and T4 DNA liga...
The karyotype of the cultivated rye (Secale cereale) is one of the complex plant genomes whose chromosomes are difficult to discriminate because of their similar size. Aim. The purpose of this study is to reveal the chromosome variable sites using DNA repetitive sequences as probes and to define a possibility of sorting rye chromosomes. Methods. Fl...
Precise chromosome segregation is vital for speciation and hybrid formation. The aim of this work was to study the chromosomes behavior and inheritance of maternal and paternal genomes in Arabidopsis regenerants obtained from in vitro cultured cells on the medium with para-fluorophenyalanine (PFPA). The Arabidopsis thaliana model hybrid between Col...
The goal of the work was, using the database «The Arabidopsis Information Resource» TAIR, to select 12 SSLP-markers distributed along the Arabidopsis chromosomes and chromosome arms, to optimize the conditions of amplification of each fragment and for simultaneous amplification of several fragments. For identification of A. thaliana ecotypes and th...
There is plenty of evidence for genome instability in somatic tissues due to mitotic disorders. This short review focuses on the phenomena of chromosome number reduction in somatic plant cells referred to in the literature by different names as somatic reduction, somatic segregation, meiosis-like reduction, prophase grouping and assortment mitosis.