Olga Sayanova

Olga Sayanova
Biotechnology and Biological Sciences Research Council | BBSRC · BCH

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104
Publications
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Publications

Publications (104)
Article
The Brassicaceae Camelina sativa (Gold‐of‐pleasure) is now an established niche crop, as well as being used as a transgenic host for a range of novel seed traits. Most notable of these is the accumulation of omega‐3 long chain polyunsaturates such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), fatty acids normally only found in mari...
Article
Full-text available
The unicellular marine diatom Phaeodactylum tricornutum accumulates up to 35% eicosapentaenoic acid (EPA, 20:5n3) and has been used as a model organism to study long chain polyunsaturated fatty acids (LC‐PUFA) biosynthesis due to an excellent annotated genome sequence and established transformation system. In P. tricornutum, the majority of EPA acc...
Article
Full-text available
Background: Oleaginous microalgae represent a valuable resource for the production of high-value molecules. Considering the importance of omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) for human health and nutrition the yields of high-value eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) require significant improvement to mee...
Article
Full-text available
The transgene‐directed accumulation of non‐native omega‐3 long chain polyunsaturated fatty acids in the seed oil of Camelina sativa (Camelina) was evaluated in the field, in distinct geographical and regulatory locations. A construct, DHA2015.1, containing an optimal combination of biosynthetic genes, was selected for experimental field release in...
Preprint
Full-text available
Background: Oleaginous microalgae represent a valuable resource for the production of high value molecules. Considering the importance of omega-3 long chain polyunsaturated fatty acids (LC-PUFAs) for human health and nutrition the yields of high value eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) require significant improvement to meet...
Preprint
Full-text available
Background: Oleaginous microalgae represent a valuable resource for the production of high value molecules. Considering the importance of omega-3 long chain polyunsaturated fatty acids (LC-PUFAs) for human health and nutrition the yields of high value eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) require significant improvement to meet...
Article
Full-text available
The global challenges of ensuring sufficient safe and nutritious food for all are enshrined within the Sustainable Development Goals. As our planet's population continues to grow, and as the impacts of climate change and environmental pollution become more visible to all, new solutions continue to be sought as to how best address these. Transgenic...
Article
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There is an urgent requirement for sustainable sources of food and feed due to world population growth. Aquaculture relies heavily on the fish meal and fish oils derived from capture fisheries, challenging sustainability of the production system. Furthermore, substitution of fish oil with vegetable oil and fish meal with plant seed meals in aquacul...
Article
The potential for using genetic modification (GM) to enhance the nutritional composition of crops (for either direct human consumption or as animal feed) has been recognized since the dawn of the GM era, with such ‘output’ traits being considered as distinct, if not potentially superior, to ‘input’ traits such as herbicide tolerance and insect resi...
Chapter
Interest in microalgal biotechnology for the production of bioactive and nutritional ingredients has increased tremendously with the urgent need for developing renewable bioresources. Microalgae represent a huge and still insufficiently tapped resource of LC-PUFA for human nutrition and health-related applications. Photosynthetic omega-3 and omega-...
Article
Facing a bottleneck in the growth of aquaculture, and a gap in the supply and demand of the highly beneficial n -3 long-chain PUFA (LC-PUFA), sustainable alternatives to traditional marine-based feeds are required. Therefore, in the present trial, a novel oil obtained from a genetically engineered oilseed crop, Camelina sativa , that supplied over...
Article
Genetic engineering can be the solution to achieve the economically feasible production of microalgal based biofuels and other bulk materials. A good number of microalgal species can grow mixotrophically using acetate as carbon source. Moreover, experimental evidence suggests that the biosynthesis of acetyl-CoA could be a limiting step in the compl...
Article
Full-text available
There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineer...
Article
Diatoms are responsible for up to 40% of the carbon fixation in our oceans. The fixed carbon is moved through carbon metabolism towards the synthesis of organic molecules that are consumed through interlocking foodwebs, and this process is strongly impacted by the abiotic environment. However, it has become evident that diatoms can be used as ‘plat...
Article
Full-text available
We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Delta 5-elongase from Ostreococcus tauri was co-expre...
Article
Full-text available
Plant seed lipid metabolism is an area of intensive research, including many examples of transgenic events in which oil composition has been modified. In the selected examples described in this review, progress towards the predictive manipulation of metabolism and the reconstitution of desired traits in a non-native host are considered. The advanta...
Article
Full-text available
Background: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed. Objective: The main objective was to investigate the ability of genetically engineered...
Article
Full-text available
The marine diatom Phaeodactylum tricornutum can accumulate up to 30% of the omega-3 long chain polyunsaturated fatty acid (LC-PUFA) eicosapentaenoic acid (EPA) and, as such, is considered a good source for the industrial production of EPA. However, P. tricornutum does not naturally accumulate significant levels of the more valuable omega-3 LC-PUFA...
Article
The global consumption of fish oils currently exceeds one million tonnes, with the natural de novo source of these important fatty acids forming the base of marine foodwebs. Here we describe the first field-based evaluation of a terrestrial source of these essential nutrients, synthesised in the seeds of transgenic Camelina sativa plants via the he...
Article
Full-text available
1An alternative, sustainable source of omega‐3 long chain polyunsaturated fatty acids is widely recognized as desirable, helping to reduce pressure on current sources (wild capture fisheries) and providing a de novo source of these health beneficial fatty acids. This review will consider the efforts and progress to develop transgenic plants as terr...
Patent
The invention provides isolated nucleic acid molecules which encodes a novel fatty acid nECR. The invention also provides recombinant expression vectors containing nECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs)...
Article
Full-text available
Currently, one alternative for dietary fish oil (FO) in aquafeeds are vegetable oils (VO) that are devoid of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). Entirely new sources of n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids through de novo production is a potential solution to fill the gap between sup...
Article
Full-text available
The synthesis and accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa is demonstrated using the so-called alternative pathway. This aerobic pathway is found in a small number of taxonomically unrelated unicellular organisms and utilizes a C18 Δ9-elongase to generate C20 PUFAs. Here, we evaluated four differe...
Article
Full-text available
For humans a daily intake of up to 500mg omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) is recommended, amounting to an annual requirement of 1.25 million metric tonnes (mt) for a population of 7 billion people. The annual global supply of n-3 LC-PUFA cannot meet this level of requirement and so there is a large gap between supply a...
Article
Full-text available
The world faces considerable challenges including how to produce more biomass for food, feed, fuel and industrial feedstock without significantly impacting on our environment or increasing our consumption of limited resources such as water or petroleum-derived carbon. This has been described as sustainable intensification. Oleaginous crops have the...
Article
Full-text available
Omega-3 fatty acids are characterized by a double bond at the third carbon atom from the end of the carbon chain. Latterly, long chain polyunsaturated omega-3 fatty acids such as eicosapentaenoic acid (EPA; 20:5Δ5,8,11,14,17) and docosahexanoic acid (DHA; 22:6 Δ4,7,10,13,16,19), which typically only enter the human diet via the consumption of oily...
Patent
The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to...
Article
Full-text available
We have engineered the diatom Phaeodactylum tricornutum to accumulate the high value omega-3 long chain polyunsaturated fatty acid docosahexaenoic acid (DHA). This was achieved by the generation of transgenic strains in which the Δ5-elongase from the picoalga Ostreococcus tauri was expressed to augment the endogenous fatty acid biosynthetic pathway...
Article
Full-text available
In order to identify novel genes encoding enzymes involved in the biosynthesis of nutritionally important omega-3 long chain polyunsaturated fatty acids, a database search was carried out in the genomes of the unicellular photoautotrophic green alga Ostreococcus RCC809 and cold-water diatom Fragilariopsis cylindrus. The search led to the identifica...
Article
Full-text available
Omega-3 (also called n-3) long-chain polyunsaturated fatty acids (≥C20; LC-PUFAs) are of considerable interest, based on clear evidence of dietary health benefits and the concurrent decline of global sources (fish oils). Generating alternative transgenic plant sources of omega-3 LC-PUFAs, i.e. eicosapentaenoic acid (20:5 n-3, EPA) and docosahexaeno...
Data
Total fatty acid composition of oil seeds from wild-type and transgenic C. sativa plants.
Data
Full-text available
Comparison of C. sativa wild-type, RRes_EPA and RRes_DHA plants at 6 and 10 weeks old. Figure S2. Analysis of major phospholipid species in wild-type and engineered mature C.sativa seed. Figure S3. LC-MS/MS + MRM analysis of TAG from C. sativa engineered for production of EPA and/or DHA. Figure S4. Parallel analysis (GC-FID) of FAMEs derived from t...
Article
An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid metabolism. Successive iterations...
Article
This article will focus on the modification of plant seed oils to enhance their nutritional composition. Such modifications will include C18 Δ6-desaturated fatty acids such as γ-linolenic and stearidonic acid, omega-6 long-chain polyunsaturated fatty acids such as arachidonic acid, as well as the omega-3 long-chain polyunsaturated fatty acids (ofte...
Article
The synthesis and accumulation of long chain polyunsaturated fatty acids such as eicosapentaenoic acid has previously been demonstrated in the seeds of transgenic plants. However, the obtained levels are relatively low, indicating the need for further studies and the better definition of the interplay between endogenous lipid synthesis and the non-...
Article
Full-text available
Omega-3 (ω-3) very long chain polyunsaturated fatty acids (VLC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5 Δ5,8,11,14,17) and docosahexaenoic acid (DHA; 22:6 Δ4,7,10,13,16,19) have been shown to have significant roles in human health. Currently the primary dietary source of these fatty acids are marine fish; however, the increasing demand for f...
Article
The role of acyl-CoA-dependent Δ6-desaturation in the heterologous synthesis of omega-3 long-chain polyunsaturated fatty acids was systematically evaluated in transgenic yeast and Arabidopsis thaliana. The acyl-CoA Δ6-desaturase from the picoalga Ostreococcus tauri and orthologous activities from mouse (Mus musculus) and salmon (Salmo salar) were s...
Article
Growing evidence suggests that omega-3 long chain polyunsaturated fatty acids (VLC-PUFAs), especially eicosapentaenoic acid (EPA; 20:5Δ5,8,11,14,17) and docosahexaenoic acid (DHA; 22:6Δ4,7,10,13,16,19) play critical roles in human health and development. VLC-PUFAs are mainly found in fish, some fungi, marine bacteria and microalgae. Currently, the...
Article
It is now accepted that omega-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA; 20:5Δ5,8,11,14,17) and docosahexaenoic acid (DHA, 22:6Δ4,7,10,13,16,19) play important roles in a number of aspects of human health, with marine fish rich in these beneficial fatty acids our primary dietary source. However, over-fishing and concerns...
Article
Full-text available
Enormous progress has been made in understanding genetic components required for the biosynthesis and modifica - tion of fatty acids in plants, thus facilitating the identification of sequences responsible for unusual fatty acid accumula - tion. Perhaps surprisingly, many early attempts to engineer the fatty acid composition of transgenic plants pr...
Article
Dietary omega-3 polyunsaturated fatty acids have a proven role in reducing the risk of cardiovascular disease and precursor disease states such as metabolic syndrome. Although most studies have focussed on the predominant omega-3 fatty acids found in fish oils (eicosapentaenoic acid and docosahexaenoic acid), recent evidence suggests similar health...
Article
Full-text available
The seed oil of Anemone leveillei contains significant amounts of sciadonic acid (20:3Delta(5,11,14); SA), an unusual non-methylene-interrupted fatty acid with pharmaceutical potential similar to arachidonic acid. Two candidate cDNAs (AL10 and AL21) for the C(20) Delta(5cis)-desaturase from developing seeds of A. leveillei were functionally charact...
Article
Full-text available
The marine parasitic protozoon Perkinus marinus synthesizes the polyunsaturated fatty acid arachidonic acid via the unusual alternative Delta8 pathway in which elongation of C18 fatty acids generates substrate for two sequential desaturations. Here we have shown that genes encoding the three P. marinus activities responsible for arachidonic acid bi...
Article
Full-text available
The free-living soil protozoon Acanthamoeba castellanii synthesizes a range of polyunsaturated fatty acids, the balance of which can be altered by environmental changes. We have isolated and functionally characterized in yeast a microsomal desaturase from A. castellanii, which catalyzes the sequential conversion of C(16) and C(18) Delta9-monounsatu...
Article
cDNA clones encoding cytochrome b(5) fusion desaturases were isolated from Primula cortusoides L. and Primula luteola Ruprecht, species previously shown to preferentially accumulate either n-6 or n-3 Delta6-desaturated fatty acids, respectively. Functional characterisation of these desaturases in yeast revealed that the recombinant Primula enzymes...
Chapter
IntroductionLC-PUFAs in Human HealthThe Biosynthesis of LC-PUFAsCharacterization of LC-PUFA Biosynthetic PathwaysThe Successful Synthesis of C20 PUFAs in Transgenic PlantsFuture Directions and ProspectsConclusions AcknowledgmentsReferences
Article
A cDNA encoding a C20 Delta8-desaturase was isolated from the free-living soil amoeba, Acanthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A. castellanii C20 Delta8-desaturase showed similarity to other microsomal front-end desaturases, but the N-terminal domain contained a variant form...
Article
Very long-chain polyunsaturated fatty acids are vital components of the human diet, playing important multiple roles in optimal health and development. Current dietary sources, in the form of fish oils, are in decline, and an alternative sustainable source is required. Recent attempts to engineer transgenic plants with the capacity to synthesize th...
Article
There is now considerable evidence of the importance of n-3 long-chain PUFA in human health and development. At the same time, the marine fish stocks that serve as the primary sources of these fatty acids are threatened by continued over-exploitation. Thus, there is an urgent need to provide a sustainable alternative source of the n-3 long-chain PU...
Article
Analysis of a draft nuclear genome sequence of the diatom Thalassiosira pseudonana revealed the presence of 11 open reading frames showing significant similarity to functionally characterized fatty acid front-end desaturases. The corresponding genes occupy discrete chromosomal locations as determined by comparison with the recently published genome...
Article
Long-chain PUFA such as eicosapentaenoic and docosahexaenoic acids are prevalent in fish oils, and these compounds have been demonstrated to play important roles in human health and nutrition. In particular, these n-3/omega-3 long-chain PUFA provide protection from cardiovascular disease and a collection of symptoms (termed metabolic syndrome) asso...
Article
The nutritional importance of long chain polyunsaturated fatty acids (LC-PUFAs) is now well-established, with these lipids playing roles in human development and the prevention of various diseases. In particular, n-3 polyunsaturated fatty acids are protective against cardiovascular disease (CVD) and risks associated with metabolic syndrome. In view...