Olga Leonidovna Gorbunova

• Russian Academy of Sciences

The peptide hormone kisspeptin, produced by neurons of the hypothalamus anterior zone, is a key regulator of the gonadostat formation and reproductive function, and has immunoregulatory activity. During pregnancy, kisspeptin is produced by the placenta, presents in the peripheral blood and affects on immune cells that express specific receptors for...

The functions of peripheral blood NK cells change significantly during pregnancy, which is mainly due to the inhibition of their cytotoxicity. The functional activity of NK cells is directly related to their metabolic status, but these changes in physiological pregnancy have not been studied. The aim of this work is to study the expression of Glut-...

Physiologically, pregnancy is accompanied by profound changes in the functions of cells of both adaptive and innate immunity, which is necessary to prevent rejection of a semi-allogenic fetus and preserve the protective properties of the mother’s body. Objective: to investigate the metabolic activity of CD16hi and CD16int subpopulations of peripher...

Аннотация.Галектинами называют семейство β-галактозид-связывающих низкомолекулярных белков (лектинов), гомологичных по аминокислотной последовательности углевод-связывающего сайта. Галектины взаимодействуют с гликанами на поверхности клеток и участвуют в регуляции функциональной активностибольшинства клеток организма человека. Галектин-9 играет важ...

РЕЗЮМЕ Цель-оценить метаболическую активность разных субпопуляций натуральных киллеров (NK-клеток) периферической крови в I и в III триместрах физиологически протекающей беремен-ности (ФПБ). Материал и методы. В исследование включены здоровые женщины в I или III триместрах ФПБ. Группу сравнения составили условно-здоровые небеременные женщины в фол...

Galectin-9 is a b-galactoside binding lectin with expressed immunoregulatory activity. During pregnancy galectin-9 is produced by trophoblast cells and regulates the function of natural killer (NK) cells at the maternal-fetal interface via binding to Tim-3 (T-cell Ig and mucin domain-containing protein 3) molecules. Natural killer (NK) lymphocytes...

Pregnancy is a phenomenon of natural semi-allogeneic transplantation, since the fetus is half alien due to the expression of paternal antigens. It was found that the hypothalamic hormone kisspeptin during pregnancy is produced by the syncytiotrophoblast of the placenta and participates in the formation of a new specific hormonal background. Several...

Natural killer (NK) lymphocytes belong to the innate lymphoid cells, which have a cytotoxic effect on target cells and are capable of producing a large number of regulatory factors (cytokines, chemokines). During pregnancy, the peripheral blood CD56brightCD16-NK cells migrate to the uterus and transform into decidual (d)NK cells. dNK have a toleran...

Natural killer cells (NK) and T-lymphocytes with NK functions (NKT) are the leading effectors of the mother’s immune tolerance to a semi-allogeneic fetus and have a fetal trophic effect during physiological pregnancy. Tim-3 (T-cell Ig and mucin domain-containing protein 3) and CD9 molecules play a critical role in the immunoregulatory and fetal tro...

При физиологически протекающей беременности в матке нарастает количество натуральных киллеров (NK) и Т-лимфоцитов с функциями NK (NKT), экспрессирующих молекулы Tim-3, CD49a, CD9, которые играют критическую роль в реализации иммунорегуляторной и фетотрофической функции. Учитывая возможность образования децидуальной популяции из клеток периферическо...

Physiological pregnancy is characterized by significant changes in the phenotype and functions of peripheral blood lymphocytes. Thus, the number of lymphocytes migrating from peripheral blood in the uterus and expressing Tim-3, CD49a, CD9 molecules which limit their cytotoxicity and participate in their migration, is significantly increased in the...

Hypothalamic hormone kisspeptin is also produced by placental syncytiotrophoblast. Blood leukocytes express a specific membrane receptor of kisspeptin (KISS-1R). Since kisspeptin-54 enters the bloodstream during pregnancy, the hormone has a systemic effect on the leukocytes only in this period. Earlier we demonstrated that kisspeptin-54 enhances th...

The work is focused on physiological role of the hormone kisspeptin produced by neurons of the hypothalamus anterior zone, which is a key regulator of reproduction processes. Role of the hormone in transmission of information on metabolic activity and induction of the secretion of gonadotropin-releasing hormone (GnRH) by the hypothalamus that deter...

The aim of the work was to examine an interaction between neutrophils conditioned with chorionic gonadotropin, estriol, kisspeptin, leptin, ghrelin and commensal and uropathogenic Escherichia coli biofilms. Peripheral blood neutrophils isolated from healthy non-pregnant women in the first phase of the menstrual cycle (n = 8) were cultured in vitro...

Изучено влияние гормонов (хорионического гонадотропина, эстриола, лептина, грелина, кисспептина) в концентрациях, сопоставимых с их содержанием в периферической крови при беременности, в регуляции процессов клеточной дифференцировки в тимусе. Установлено, что исследуемые гормоны разнонаправленно регулируют функциональную активность миелоидных и пла...

Изучали влияние эстрадиола (Е2), прогестерона (P4), онкостатина М (OSM) на процессы дифференцировки CD4+ T-клеток в T регуляторные (Treg) лимфоциты или T-хелперы 17 (Th17). Также исследовали возможность ревизии T-клеточного рецептора в этих субпопуляциях с помощью оценки экспрессии рекомбиназы RAG-1. Е2 в концентрации, характерной для I триместра б...

The effect of estradiol (E2), progesterone (P4), and oncostatin M (OSM) on the differentiation of CD4⁺ T cells to T regulatory (Treg) lymphocytes and T helpers 17 (Th17) was investigated. The possibility of revision of the T cell receptor in these subpopulations by evaluating the expression of RAG-1 recombinase was also studied. E2 at concentration...

We studied the effect of estriol, chorionic gonadotropin, oncostatin M, and hormone-cytokine combinations on the expression of recombinase RAG-1 in T-regulatory (Treg) and T helper 17 (Th17) lymphocytes. It was found that estriol in a concentration corresponding to the first trimester of pregnancy increased the level of Treg (CD4+FoxP3+) cells and...

We studied the effect of hormones estriol, ghrelin, kisspeptin, and chorionic gonadotropin in concentrations corresponding to their content in the peripheral blood in each trimester of pregnancy on the expression of membrane molecules on myeloid and plasmacytoid dendritic cells of the thymus. It was found that thymic myeloid dendritic cells are sen...

The effects of kisspeptin, an important regulator of reproductive function, and leptin, a classical metabolic hormone, on the formation of inducible regulatory T cells (iTreg) and T helper cells that produce interleukin 17 (Th17), as well as on the activity of indoleamine 2,3-dioxygenase (IDO) have been studied. Both hormones are actively produced...

We investigated the effect of human chorionic gonadotropin, estriol, leptin, ghrelin, and kisspeptin on the microRNA expression in separated NK cells. All of these hormones are able to effectively modulate the expression of microRNAs both stimulating and suppressing the cytotoxic potential of NK cells and thereby indirectly regulate the functions o...

Изучено влияние пептидных гормонов лептина и грелина на экспрессию мембранных молекул и продукцию цитокинов NK-клетками периферической крови in vitro. Действие гормонов оценивали в концентрациях, отражающих их содержание в периферической крови на разных триместрах бере- менности. Установлено, что значимые эффекты гормоны оказывают лишь в концентрац...

Исследовали влияние хорионического гонадотропина, эстриола (Е3), лептина, грелина и кисспеп- тина на внутриклеточную экспрессию перфорина, гранзимов A и B в сепарированных NK-клетках. Все исследуемые гормоны, за исключением Е3, способны модулировать экспрессию цитотоксиче- ских ферментов NK-клеток посредством угнетения экспрессии наиболее активных...

The influence of leptin and ghrelin, as well as their combined effects, on the expression of membrane molecules and cytokine production by NK cells from peripheral blood was studied in vitro. The effects of hormones were assayed at the concentrations corresponding to their peripheral blood levels in the course of physiological pregnancy. It was est...

The effects of chorionic gonadotropin, estriol (E3), leptin, ghrelin, and kisspeptin on the intracellular expression of perforin, granzyme A, and granzyme B was studied in separated NK cells. All studied hormones except E3 are could modulate the expression of cytotoxic enzymes in NK cells by suppression of the expression of the most active proapopt...

Исследовали влияние хорионического гонадотропина, эстриола, лептина, грелина и кисспептина на экспрессию микроРНК в сепарированных NK-клетках. Все исследуемые гормоны способны эффективно модулировать экспрессию микроРНК, как стимулирующих, так и угнетающих цитотоксический потенциал NK-клеток, тем самым опосредованно регулировать функции данных лимф...

The influence of chorionic gonadotropin (CG) and estriol (Е3) at the concentrations typical of pregnancy on the expression of phenotypic markers and cytokine production by separated NK cells has been studied. It has been found that these hormones increase the percentage of CD56bright L-selectin⁺ NK cells, but also stimulate the expression of the in...

Both leptin and ghrelin used separately at the concentrations corresponding to trimesters II–III of pregnancy increase the number of CD56bright NK cells in mononuclear cell suspension; their combination also enhances the L-selectin expression on the surface of these cells in the culture. These hormones do not affect the production of TGF-β1, IL-17А...

Molecular mechanisms of the immunomodulatory effects of hormone kisspeptin on the formation and functional activity of inducible regulatory T cells (iTreg) and lymphocytes T helpers-17 (Th17) were studied using inhibitory analysis and direct measurements of the intracellular cAMP level. We found that kisspeptin in concentrations typical of the II a...

Лептин и грелин в концентрациях, соответствующих II–III триместрам беременности, по отдельности увеличивают число CD56bright NK-клеток в суспензии мононуклеаров, а при совместном вве-дении в культуру усиливают экспрессию L-селектина на поверхности этих клеток. Эти гормоны невлияют на выработку TGF-β1, IL -17А, IFN-γ NK-клетками и угнетают продукцию...

Тимус является центральным органом иммунитета, где происходит формирование основных эффекторных и регуляторных субпопуляций Т-лимфоцитов. Беременность сопровождается стероид-индуцированной инволюцией тимуса. Подобная гормон-зависимая инволюция тимуса наблюдается также при стрессе, старении, ряде аутоиммунных заболеваний. При беременности плацента п...

The effect of kisspeptin at concentrations typical of pregnancy on the functional activity of isolated cytokine-primed NK cells has been investigated. The hormone has been shown to promote an increase in the proportion of CD56bright NK cells, as well as an increase in the L-selectin expression on the cell surface. Assessment of cytokine levels has...

The effects of human chorionic gonadotropin (hCG), estriol, kisspeptin, leptin and ghrelin on the expression of homing molecules CCR7 and L-selectin on NK-cells was studied. It was established that hCG, estriol and leptin increase L-selectin expression on CD56bright cells. Leptin, ghrelin and kisspeptin reduce CCR7 expression on NK-cells. Thus, the...

Установлено, что в концентрациях, соответствующих I триместру беременности, ХГ, Е3 и кисспептин повышают количество CD56bright клеток. Одновременно Е3 и кисспептин, но не ХГ, снижают процент CD56dim лимфоцитов. Дополнительно кисспептин снижает количество NK-клеток, экспрессирующих как активационные (NKG2D), так и ингибиторные (LILRB) молекулы, а ХГ...

The effects of hormones associated with gestation (human chorionic gonadotropin (hCG), estriol (E3), Kisspeptin, leptin and ghrelin) on the expression of CD56, NKG2D, NKG2A and LILRB NK-cells. It was established that at concentrations corresponding to the I trimester of pregnancy, hCG, and E3 Kisspeptin increase the number of CD56bright cells. Simu...

Investigated oligopeptides (LQGV, AQGV, VLPALP) are products of catabolism molecule chorionic gonadotropin (hCG), and possess structural relatedness to the -subunit of the hormone. In the works of Khan N.A. & Benner R. (2011) demonstrated that these oligopeptides have expressed anti-inflammatory and anti-shock effects and block the development of...

It was investigated the effects of reproductive hormones (hCG, E3, leptin, ghrelin, kisspeptin) on phenotypic maturation of intact NK-cells. It was found that E3 in concentrations corresponding to the first half of the physiological pregnancy reduced the activity of NK-cells, ghrelin and kisspeptin did not affect it, while hCG and leptin promoted t...

Effect of recently opened hormone kisspeptin that is detected in peripheral blood only in pregnancy was analyzed against the monocyte functional activity. It was revealed that kisspeptin in concentration corresponding to III trimester of pregnancy promoted the phagocyte activity of monocytes and in all of concentrations under study favored the incr...

We studied the mechanisms of induction of recombinase activity in peripheral T cells of patients with autoimmune type 1 diabetes mellitus. It was shown that the presence of CD40 on T cell membrane (not typical of these cells) is crucial for this process: expression of recombinase RAG-1 in diabetic patients was detected primarily in αβTCR(+)CD40(+)...

The explanation of mother immune system toler� ance to genetically allogenic fetus is a topical issue of reproductive immunology, whose important branch is the study of the role of hormones in immune system control. In terms of maintaining normal pregnancy, chorionic gonadotropin (CG) is the key hormone having evident immunomodulatory properties [1...

The effects of chorionic gonadotropin (CG) and CG-induced hormonal background on the phagocytic activity of peripheral blood leukocytes and peritoneal macrophages were studied in female Swiss mice. In vitro, the direct immunosuppressing effect of CG on phagocytes depended on the function of L-type Ca2+ channels and was increased by an additional pa...

Experiments on male Swiss mice studied the effect of chorionic gonadotropin (CG) on the phagocytic activity of peripheral blood leukocytes and peritoneal macrophages, by taking into account the stages of formation of a humoral immune response. The administration of CG was found to stimulate the splenic production of antibody-forming cells (AFC) and...

Сконструирована система для «сэндвич»-варианта дот-блотинга при безынструментальном определении антител любой специфичности. Визуализация связанных антител осуществляется с помощью неферментного диагностикума, изготовленного путем ковалентного связывания белка G с частицами коллоидного углерода. Разработанная система анализа обладает высокой чувств...

Pregnancy is a phenomenon of natural transplantation, because the extraembryonic membranes, as well as the embryo, express the histocompatibility antigens of father’s haplotype and may serve as a potential target for the immunocompetent mother’s cells [7, 8]. The main hormone of pregnancy, chorionic gonadotrophin (CG), is synthesized by the placent...