Olga Alekseeva

Olga Alekseeva
Sechenov Institute of Evolutionary Physiology and Biochemistry · Laboratory of Comparative Physiology of Respiration

PhD

About

63
Publications
3,603
Reads
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143
Citations
Citations since 2016
29 Research Items
122 Citations
201620172018201920202021202205101520
201620172018201920202021202205101520
201620172018201920202021202205101520
201620172018201920202021202205101520
Introduction
Olga Alekseeva currently works at the Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences. Olga does research in Neurology, Respiratory Medicine and Toxicology. Their most recent publication is 'Neuroglobin, an oxygen-binding protein in the mammalian nervous system (localization and putative functions)'.
Additional affiliations
August 1992 - August 2017
Russian Academy of Sciences
Position
  • Researcher
August 1992 - August 2017
Russian Academy of Sciences
Position
  • Researcher

Publications

Publications (63)
Article
Immunocytochemical and confocal laser microscopy were used to study the structural organization and spatial distribution of microgliocytes in the molecular layer of the cerebellar cortex in fi ve adult male rabbits. Reactions for the microgliocyte marker Iba-1 were highly specifi c, though until recently it was impossible to detect these cells sele...
Article
Full-text available
Assessment of safety, viability, and functional state of nerve cells is the major problem in studies of experimental effects on various structures of vertebrate brain and in search for correlation between structural abnormalities and changes in physiological parameters. Such an assessment is possible with applying an immunocytochemical reaction to...
Article
Based on the key statistical figures analysis on fires in Ukraine during 2000-2013, a dynamic multifactor model has been built revealing the dependence of direct losses as a result of fires on the number of fires, the number of deaths caused by fires and the number of injured in fires. The problematic issues are outlined and solutions on the organi...
Article
We studied the reaction of rat hippocampal microgliocytes to hyperbaric oxygen at a pressure of 5 ata (absolute atmosphere). Immunohistochemical analysis with selective macrophage marker CD68 (ED1) and microglial marker Iba-1 allowed separate analysis of these two cell populations. It was shown that macrophages do not significantly contribute to re...
Article
The purpose of the scientific article was to study the neurophysiological mechanisms of the occurrence of convulsive activity when breathing oxygen under high pressure under conditions of hyperbaric oxygen and the role of the glutamatergic system in this process. Research methods : Wistar rats, males, weighing 200–250 g. in the amount of 20 were us...
Article
Analysis of the age-related dynamics of olfactory behavior in the odor preference and food search testsshowed that all male Wistar rats, regardless of age, preferred valerian essential oil, whose components have the properties of pheromones in rodents, when given a selection of eight essential oils; young rats displayed better food-seeking results...
Article
Kolmer cells (epiplexus macrophages) constitute the least studied population among the phagocytosing cells in the mammalian brain. We report here studies of the reactions of these cells to ischemic brain injury induced by brief termination of the blood supply in the basin of the middle cerebral artery. These studies used rats of spontaneously hyper...
Article
Respiration of hyperbaric oxygen (HBO2) suppresses the synthesis of γ-aminobutyric acid (GABA) in the brain, leading to weakening of inhibitory GABAergic neurotransmission and the development of convulsive syndrome of the epileptic seizure type. We report here our testing of the hypothesis that inhibition of GABA transporters might compensate for i...
Article
Experiments using C57BL/6J mice 3 h after administration of agouti gene-related peptide (AgRP) fragments 83-132 and 25-51 into the midbrain identified dose-dependent inhibitory effects on dopaminergic neurons. Staining of midbrain sections using an immunohistochemical method with specific antibodies showed that fragment AgRP 83-132 induced signific...
Article
Full-text available
Hyperbaric oxygen (HBO2) is acutely toxic to the central nervous system, culminating in EEG spikes and tonic-clonic convulsions. GABA enhancers and sodium channel antagonists improve seizure latencies in HBO2 when administered individually, while combining antiepileptic drugs from different functional classes can provide greater seizure latency. We...
Article
The cardiovascular system of vertebrates, including humans, is well known to respond to hyperoxia by vasoconstriction, bradycardia and decreased contractility of the left heart ventricle. We hypothesized that all of these responses are components of the baroreflex that regulates blood pressure and circulation in hyperoxia. To test this hypothesis,...
Article
Full-text available
The review summarizes data on microglyocytes in the brain of mammals. General characteristics and features of their structure, functions and molecular organization at different ontogenetic stages are addressed. A special focus is on various hypotheses of the origin of microglia and current ideas about the involvement of microglyocytes in the regula...
Article
Neurogenesis in the subgranular zone (SGZ) of the mammalian hippocampus is well known to occur throughout the life span. Astrocytes in this specialized proliferative zone are supposed to have properties of progenitor cells. Structural features of these cells and their interspecies differences remain understudied, while data on the structural organi...
Article
Full-text available
The review summarizes current data on neuroglobin, the heme-containing protein discovered in mammalian nerve cells in 2000. It presents general characteristics of neuroglobin as well as data on its evolutionary changes and expression across different taxa. Neuroglobin distribution in specific brain structures and outside the brain is described. The...
Article
Full-text available
Iba-1 protein is a widely recognized marker for microglial cells and was previously observed by the authors in microgliocyte nuclei. The aim of the present work was to refine these data using immunocytochemistry and confocal laser microscopy methods. Studies were performed on human brain fragments (cortex, striatum, substantia nigra, red nucleus) (...
Article
Full-text available
Cytoskeletal intermediate filaments (IF) are composed of proteins able to form homo- and heterodimers, while their repertoire can change during cell differentiation. Data on the IF protein composition in tanycytes lining the mammalian third cerebral ventricle are still discrepant. The aim of this study was to investigate age-related changes in the...
Article
Neuroglobin is an iron-containing protein, most abundant in the vertebrate nervous system. Since neuroglobin is able to bind oxygen reversibly owing to the heme prosthetic group, it was believed that its function is an intercellular transport of oxygen in the nervous system and accumulation of oxygen for energy supply of cells in hypoxic conditions...
Article
Changes in electrical activity of the neocortex after prenatal hypoxia (day 14 of embryogenesis, E14, 7% O2 for 3 h) and intracortical microinjection of epileptogenic 4-aminopyridine (4-AP) were studied in adult (3-month-old) rats. The frequency–time parameters of electrocorticogram (ECoG) were analyzed during sleep and wakefulness as well as in a...
Article
The role of orexin in the organization of the sleep–wake cycle (SWC) is well known. The aim of this study was to examine the timing of the orexinergic system formation in rat postnatal ontogenesis and to assess the role of orexin A in the SWC organization under normal conditions and after prenatal hypoxia undergone on days 14 and 19 of embryogenesi...
Article
Using the methods of immunocytochemistry and confocal laser microscopy, structural organization and spatial distribution of microgliocytes in the molecular layer of the cerebellar cortex were studied in 5 adult male rabbits. Reaction to microglial cell marker Iba-1 was highly specific, while until recently their selective detection was impossible i...
Article
Neuroglobin is a recently discovered heme-containing protein located predominantly in the mammalian brain. This paper for the first time presents the data on neuroglobin distribution in human cerbellum using immunohistochemistry. Neuroglobin immunoreactivity in the cerebellum was found in all the cases studied (n = 7), although its intensity varied...
Article
Full-text available
Assessment of the integrity, viability and functional state of nerve cells is a major problem in studying the effect of experimental exposures on different brain structures in vertebrates and correlating structural abnormalities with changes in physiological parameters. Such an assessment is possible due to the use of the immunocytochemical reactio...
Article
The article presents highly reproducible and inexpensive protocol for simultaneous demonstration of glutamate decarboxylase (GAD67), the key enzyme of gamma-aminobutyric acid (GABA) synthesis and synaptophysin (SYP), a marker protein of synaptic vesicles using confocal laser microscopy. In the cerebellar cortex, GAD labels Purkinje cells and pincea...
Article
Full-text available
Glial fibrillary acidic protein (GFAP) refers to the type III intermediate filament proteins and is an essential component of the cytoskeleton in astrocytes of all vertebrates. This review presents current data on the molecular organization of GFAP in a comparative aspect. The results of most relevant studies using immunocytochemical labeling of th...
Article
Full-text available
It is known that during development of the brain, with the progress of ependendymocyte differentiation from radial gliocytes, the synthesis of nestin is stopped. However, it was shown that in the ependyma of the lateral brain ventricles nestin synthesis was resumed in response to ischemic injury. The aim of the present study was to test the hypothe...
Article
Full-text available
Brain catecholaminergic neurons belong to the most extensively studied populations of nerve cells. Presence of a pigment neuromelanin in their cytoplasm is a specific morphological feature of these neurons in many mammalian species. Elucidation of the role of neuromelanin is of importance for comparative neurobiology, as it is absent in neurons of...
Article
The goal of the study was to identify the subependymal microglial cells of the III ventricle of the rat brain and to determine their structural characteristics. The sections of the brain of intact Wistar (n = 3) and Sprague-Dawley (n = 3) male rats were studied using the methods of immunocytochemistry and confocal laser microscopy. Subependymal mic...
Article
Full-text available
Using Perls’ histochemical method, iron was revealed in the brain structures of human and rat. Iron accumulation was observed in perivascular areas and neuropil of substantia nigra as well as in white matter of cerebellum. After diaminobenzidine enhancement of histochemical reaction, the iron was revealed in nucleoli of many neurons, which is descr...
Article
Full-text available
Studies of the structural-functional organization of the boundaries of CNS areas address a fundamental task in neurobiology. Given the contradictory data on the structure of the superficial layers of the mammalian neocortex, there is value in undertaking detailed investigations of the structures and cytochemical organization of astrocytes – the mai...
Article
Importance of study of astrocytes for fundamental biology and medicine is due their key role in formation of the brain barrier system. On taking into consideration the controversial data on structure of the mammalian neocortex superficial layers, of great actuality are the comparative studies of the structural and cytochemical organization of astro...
Article
Full-text available
The aim of the present work was to study the distribution of cells expressing Musashi-1 (Msi-1) protein, which is regarded as a marker for neural stem cells, in the rat telencephalon. These cells were found to be concentrated in the subventricular proliferative zone and diffusely distributed in the striatum and hippocampus. In addition, intense rea...
Article
Full-text available
Importance of study of astrocytes for fundamental biology and medicine is due to their key role in formation of the brain barrier system. On taking into consideration the controversial data on structure of the mammalian neocortex superficial layers, of great actuality are the comparative studies of the structural and cytochemical organization of as...
Article
Full-text available
The data obtained demonstrated that NO restrains ET-1 production and blunts ET-1-mediated basal cerebrovascular tone. Local hyperoxygenation of the brain tissue decreases NO availability, supeoxide production, suppresses NO-mediated vascular tone and facilitates ET-1-mediated vasoconstriction.
Article
The aim of the study was to examine the distribution of cells expressing Msi-1 (Musashi-1) protein which is believed to be a marker of neural stem cells, in rat telencephalon. These cells were found to be concentrated in the subventricular proliferative zone and diffusely scattered in striatum and hippocampus. Moreover, neurons with extensive Msi-1...
Article
Full-text available
Subventricular proliferative zone is the brain area most intriguing and least understood in terms of cellular and spatial organization. The objective of this study was to focus on the structural and cytochemical organization of astrocytes of the subventricular zone of the rat brain. Astrocytes were detected immunocytochemically. The preparations we...
Article
Full-text available
A study of structural and functional organization of the boundaries separating CNS compartments is a fundamental task of neurobiology. Taking into account the contradictory data on the structure of superficial layers of mammalian neocortex, it is pertinent to study structural and cytochemical organization of astrocytes--the main components of the b...
Article
Full-text available
Experiments were carried out on the 19-day old rat embryos, the 5- and 45-day old rat pups, and the 1.4-1.5-year old rats. Phospholipids and their fatty acid composition in brain cell nuclei were studied using methods of extraction, two-dimensional thin-layer chromatography, and spectrofluorimetry. In the course of ontogenesis, the percentage of di...
Article
Full-text available
The attenuating effect of various variants of hypoxia on hyperbaric anesthesia in rats was studied. The most efficient turned out to be the daily 8-fold one-hour interval 6% hypoxia that decreased manifestation of the anesthesia by 60–67%. The immunocytochemical light optical microscopy showed that in the brain cortex after the 8th seance of such h...
Article
The spontaneous motor activity and pose reflexes of male adult rats (Wistar) were observed in the course of high pressure nitrogen compression up to 4,1 MPa. The experiments were carried out under normoxic and hypoxic conditions. Stabile rat motor cortex oxygen tension was recording during the nitrogen compression up to 7,1 MPa under normoxic condi...
Article
Full-text available
Hyperbaric oxygenation modulates cerebral blood flow affecting the development of oxygen convulsions. Before hyperbaric oxygenation-induced convulsions in rats the initial decrease in blood flow gave place to hyperemia, Po(2) increased. In rats receiving cyclooxygenase inhibitor indomethacin no convulsions were observed, blood flow and Po(2)were lo...
Article
Consumption of oxygen in rats was studied under conditions of the gas different ambient temperatures and pressure. A dependence was revealed between the neutral temperature and the high gas pressure. Mathematical model revealed an increase oxygen consumption at an increase gas pressure. The data suggest that the animal body temperature may be kept...

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