Olena Demchenko

• PhD
• Researcher at State institution "National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine

To create film materials with antimicrobial properties, silver nanoparticles are widely used, which are introduced, in particular, into polymer matrices. In this work, a mixture of polylactide-polycaprolactone polymers, taken in a mass ratio of 80–20, was used as a polymer base. These studies will further contribute to the development of new safe m...

Objective: to investigate the reciprocal impact on the genome of malignant and normal human peripheral blood lymphocytes under their co-culture and the possibility to modify the effects by astaxanthin. Methods. Separate and joint/separate culturing of peripheral blood lymphocytes (PBL) of the chronic lymphocytic leukemia (CLL) patients (n = 6) and...

Objective: Study the tumor-induced bystander effect of blood cells from chronic lymphocytic leukemia (CLL)patients on non-transformed bystander cells (peripheral blood lymphocytes (PBL) of conditionally healthy individ-uals) and the possibility of its modification after the impact of ionizing radiation. Materials and methods: We carried out cocu...

Методом електрофорезу окремих клітин (сomet assay) в нейтральних умовах досліджено особливості індивідуальної радіаційно-індукованої нестабільності геному хворих на гліобластому. Встановлено, що в культурі лімфоцитів периферичної крові у двох осіб з патоморфологічно верифікованою гліобластомою (пацієнти № 1 та № 3) відсоток “комет” з високим рівнем...

ISSN 0095-4527, Cytology and Genetics, 2020, Vol. 54, No. 2, pp. 124–129. © Allerton Press, Inc., 2020. Ukrainian Text © The Author(s), 2020, published in Tsitologiya i Genetika, 2020, Vol. 54, No. 2, pp. 45–51. Evaluation of the Interaction between Malignant and Normal Human Peripheral Blood Lymphocytes Under Cocultivation and Separate Cultivat...

One of the hereditary factors affecting the development of breast and ovarian cancer is the 5382insC mutation in BRCA1 gene. But, nearly 15 % of carriers of this mutation do not manifest cancer during their lifetime. At present, the features of the development of spontaneous and induced genomic instability in asymptomatic carriers of BRCA1 mutation...

Методом електрофорезу окремих клітин (сomet assay) в нейтральних умовах досліджено вплив астаксантину на прояв ефекту свідка при сумісному культивуванні інтактних та опромінених in vitrо в дозі 0,5 Гр лімфоцитів периферичної крові людини. Показано значне зменшення виходу ДНК у культурах клітин-свідків порівняно з контролем. Це пояснюється наявністю...

Objective: To investigate an effect of astaxanthin on global DNA methylation in human peripheral blood lympho-cytes exposed to γ-radiation in vitro. Methods: Samples of whole blood were diluted with RPMI-1640 medium in proportion 1 : 10 and incubated at 37 °Cfor 3 h. Samples were exposed to γ-ray (emitter IBL-237C, dose-rate 2.34 Gy/min) in dose...

Objective: To investigate the development of chromosomal instability as a result of the radiation-induced bystandereffect in blood lymphocytes of persons from different age groups. Materials and methods: Materials of research were blood lymphocytes from 42 persons of different age (from 12 to102 years), divided into four age groups - teenagers,...

The frequency of spontaneous and X-ray-induced (0.25 Gy) in vitro chromosomal aberrations, as well as the level of chromosomal instability as a result of the bystander effect, in the blood lymphocytes of individuals aged from 12 to 102 years has been investigated using GTG staining of human metaphase chromosomes. The average group frequency of spon...

This study presents the results and comparison of the distinctive features of the modifying effect of astaxanthin (20.0 μg/mL) on γ-radiation-induced chromosomal instability in human peripheral blood lymphocytes that were irradiated in vitro (1.0 Gy) at the G0, S, and G2 stages of the cell cycle. The study showed that the effect of astaxanthin on c...

ABSTRACT Purpose: Astaxanthin (a carotenoid of xanthophyll group) satisfies all requirements demanded to potential radioprotectors. The purpose of our study was to investigate a modifying effect of astaxanthin on radiation induced genome damages in cultivating human peripheral blood lymphocytes (PBL). Materials and methods: To establish astaxanthi...

The features of astaxanthin impact (20 μg/mL) in the culture of human blood lymphocytes exposed to γ-radiation (1.0 Gy) on the G0, S, and G2 phases of the cell cycle were studied using Comet assay. Decrease in the level of DNA damages (Tail Moment index) under astaxanthin influence on lymphocytes irradiated in all stages of cell division was establ...

Objective: To identify the possibility of modification by astaxanthin the level of genome damages induced by gamma quanta in the culture of human peripheral blood lymphocytes exposed in vitro on postsynthetic (G2) phase of the first mitotic cycle. Materials and methods: Peripheral blood lymphocytes from four apparently healthy volunteers 35-51 y...

Objective: To establish and compare the frequency and spectrum of chromosome aberrations under X radiation exposure in vitro in dose 0.25 Gy peripheral blood lymphocytes of the elderly and centenarians. Material and methods: Material of cytogenetic research were peripheral blood lymphocytes from 11 elderly and 10 centenarians, which were irradia...