Okee Moses

Okee Moses
Makerere University · Department of Microbiology

Master of Science

About

46
Publications
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622
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Publications

Publications (46)
Article
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Background: Between January 2015 and July 2017, we investigated the frequency of carbapenem resistant Acinetobacter baumannii (CRAB) and carbapenem resistant Pseudomonas aeruginosa (CRPA) at the Mulago Hospital intensive care unit (ICU) in Kampala, Uganda. Carbapenemase production and carbapenemase gene carriage among CRAB and CRPA were determined...
Article
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Background Detection of Mycobacterium avium subspecies paratuberculosis (MAP) infection is key to the control of Johne’s disease. Immunohistochemistry is one of the methods of detection of MAP infection in tissues. However, unavailability of commercial antibodies that can detect the organism is a limiting factor for the use of immunohistochemistry....
Article
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Background Multidrug resistant Pseudomonas aeruginosa and Acinetobacter species are common causes of nosocomial infections worldwide. Recently we reported the occurrence of carbapenem resistant Enterobacteriaceae, P. aeruginosa and Acinetobacter species at Mulago National Referral Hospital in Kampala, Uganda, but the isolates were not analyzed for...
Article
Efficiency of artificial restriction enzymes (AREs) towards curing HIV has only been separately examined, using differing delivery vehicles. We compared the in-vitro transduction and target-mutagenesis efficiency of consortium-plasmid and adenoviral vector delivered HIV-1 pol gene targeting ZFN with CRISPER/Cas. Custom-ZFN, CRISPER-Cas-9, plasmids...
Article
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Background: In Uganda, the emerging Uganda genotype of Mycobacterium tuberculosis is the most common cause of pulmonary tuberculosis (PTB), and accounts for up to 70 % of isolates. Extrapulmonary TB (EPTB) is less studied in Uganda. Methods: Molecular characterization using deletion analysis and spoligotyping was performed on 121 M. tuberculosis i...
Article
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Background: We determined prevalence of pertussis infection and its associated host and environmental factors to generate information that would guide strategies for disease control. Methods: In a cross-sectional study, 449 children aged 3 months to 12 years with persistent cough lasting ≥14 days were enrolled and evaluated for pertussis using D...
Article
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Surveillance of the circulating Mycobacterium tuberculosis Complex (MTC) strains in a given locality is important for understanding tuberculosis (TB) epidemiology. We performed molecular epidemiological studies on sputum smear-positive isolates that were collected for anti-TB drug resistance surveillance to establish the variability of MTC lineages...
Article
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Background: The prevalence of Methicillin resistant Staphylococcus aureus (MRSA) is progressively increasing globally with significant regional variation. Understanding the Staphylococcus aureus lineages is crucial in controlling nosocomial infections. Recent studies on S. aureus in Uganda have revealed an escalating burden of MRSA. However, the S...
Data
PCR primers used in this study. (DOC)
Data
S. aureus clonal lineages based on spa typing among patients surgical site infection. *Sequence repeats with no identity in the spa server. +Not detected. (DOC)
Data
Multiplex-PCR for SCCmec Typing. Numbers 1 to 16 refer to patient isolates; –ve, negative control. (DOC)
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Study flow chart showing patient recruitment and laboratory procedures. (DOC)
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PCR-detection of the nuc gene confirming isolates as S. aureus. Numbers 1 to 31 refer to patients’ isolates; +ve, positive control; -ve, negative control. (DOC)
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Multiplex PCR in which the mecA and pvl genes were detected. Numbers 1 to 16 refer to patient isolates; +ve, positive control; -ve negative control. (DOC)
Article
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Background Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic in...
Data
Isolate profiles (bovine and human samples). R, resistant; S, susceptible, with respect to: Staphylococci: Ampicillin; Penicillin G; Trimethopprim-sulfamethoxazole; Tetracycline; Cefoxitine; Oxacillin; Amoxicillin-Clavulanate; Teicoplanin; Vancomycin; Clindamycin; Erythromycin; Nitrofurantoin; Rifampicin Enterococci: Daptomycin; Teicoplanin; Vancom...
Data
Antimicrobial resistance patterns of each staphylococcal isolate. (PDF)
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Primers for genotyping daptomycin and vancomycin resistant enterococci. (PDF)
Article
Full-text available
Rhomboids are ubiquitous proteins with unknown roles in mycobacteria. However, bioinformatics suggested putative roles in DNA replication pathways and metabolite transport. Here, mycobacterial rhomboid-encoding genes were characterized; first, using the Providencia stuartii null-rhomboid mutant and then deleted from Mycobacterium smegmatis for addi...
Data
Rhomboid family residues in MAV_1554 of M. avium codons of which were inactivated through SDM; the rest as in Figure S1. (TIFF)
Data
Generation of M. smegmatis single and double rhomboid gene mutants. Panel A: Group (a), PCR-amplification of rhomboid-encoding DNA (MSMEG_4904 and MSMEG_5036) from wild type M. smegmatis. (b), generation of marked single and double rhomboid mutants; the ∼2 kb hygromycin resistance gene (HygR) that replaced rhomboid-encoding DNA was amplified at the...
Data
Rhomboid family residues in Rv1337 codons of which were inactivated through site directed mutagenesis. In bold face: three histidine residues (H87, H92 and H204) conserved at the C-termini of most rhomboids; the rhomboid catalytic dyad (S149 and H204, in blue); the putative active site stabilizing residue in mycobacteria (F153); and the other rhomb...
Data
Catalogue of rhomboid family proteins from completed genomes of mycobacteria. Homologs are categorized as “rhomboid protease 1” or “rhomboid protease 2”. Sequences were retrieved from GenBank (PSI-BLAST algorithm) using ‘Rv0110’ and ‘Rv1337’ as queries or by blasting the mycobacterial genomes with the same queries. aWhere applicable; bRhomboid prot...
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Vectors and bacterial strains used in this study. (PDF)
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Primers used in this study. (PDF)
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Mutants of Rv1337-encoding gene generated through site directed mutagenesis. (PDF)
Article
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Staphylococcus epidermidis is often considered a non-pathogenic organism but it causes nosocomial infections. To distinguish invasive strains, comparative studies of patient and community isolates may offer some clues. We investigated the distribution of virulence determinants in patient isolates from Uganda. S. epidermidis isolates were identified...
Data
Speciation, drug susceptibility testing and detection of virulence and antimicrobial resistance genes/determinants. NA, Not applicable; ND, Not detected; MSSA, Methicillin Sensitive Staphylococcus aureus; MRSE, Methicillin Resistance Staphylococcus epidermidis.
Article
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ABSTRACT: There is limited data on Methicillin resistant Staphylococcus aureus (MRSA) in Uganda where, as in most low income countries, the routine use of chromogenic agar for MRSA detection is not affordable. We aimed to determine MRSA prevalence among patients, healthcare workers (HCW) and the environment in the burns units at Mulago hospital, an...
Data
Full-text available
The topology and location of catalytic residues in rho-1 of Drosophila. As in mycobacterial rhomboid protease 1, the catalytic residues are located in TMH4 (Gly199 and Ser201) and TMH6 (His254), while His145, His150 and Asn154 are in TMH2.
Data
ClustalW-Neighbor Joining analysis of the genes in Rv1337 cluster. Boxed (blue) are the genes that grouped with Rv1337. Essential genes in this clade are Rv1327c, Rv1327c, Rv1331, Rv1340 and Rv1344.
Data
Full-text available
ClustalW-Neighbor Joining analysis of the genes in Mjls5529 cluster. Boxed (blue) are the genes that grouped with Mjls5529, whose homologs are essential in M. tuberculosis. Several of the MTC orthologs in this clade are essential for the growth of M. tuberculosis in macrophages.
Data
The topology and location of catalytic residues in mycobacterial rhomboid protease 1 (Rv0110 orthologs). As in rho-1, the catalytic residues are located in TMH4 (Gly199 and Ser201) and TMH6 (His254), while His145, His150 and Asn154 are in TMH2.
Data
Full-text available
The topology and location of catalytic residues in mycobacterial rhomboid protease 2 (Rv1337 orthologs). The orthologs of pathogenic mycobcateria formed six TMHs, with catalytic residues in TMH4 (Gly199 and Ser201) and TMH6 (His254). His145, His150 and Asn154 are located in TMH2 as in rhomboid protease-1 (Rv0110 orthologs).
Data
Full-text available
The topology and location of catalytic residues in mycobacterial rhomboid protease 2 (Rv1337 orthologs) of nonpathogenic mycobacteria. These rhomboids formed five TMHs, with catalytic residues in TMH3 (Gly199 and Ser201) and TMH5 (His254), while His145, His150 and Asn154 are outside the TMHs (boxed).
Data
Full-text available
ClustalW-Neighbor Joining analysis of the genes in Rv0110 cluster. Boxed (blue) are the essential genes in that grouped with Rv0110 (Rv0118c, Rv0127, Rv0107c, Rv0116c, Rv0121c, Rv0132c, Rv0133 and Rv0139).
Data
The essential genes in mycobacterial rhomboid gene clusters (doc). a: According to Sassetti et al [37] and Rengarajan et al [38]. 1: Essential (for optimal growth). 2: Required for growth in macrophage. 3: Mutation slows growth.
Data
Full-text available
ClustalW-Neighbor Joining analysis of the genes in MUL4822 cluster. Boxed (blue) are the genes that grouped with MUL4822. Several of the MTC orthologs in this clade are essential for the growth of M. tuberculosis in macrophages.
Article
Full-text available
Rhomboids are ubiquitous proteins with diverse functions in all life kingdoms, and are emerging as important factors in the biology of some pathogenic apicomplexa and Providencia stuartii. Although prokaryotic genomes contain one rhomboid, actinobacteria can have two or more copies whose sequences have not been analyzed for the presence putative rh...
Article
Full-text available
The ideal identification of Staphylococcus aureus clinical isolates requires a battery of tests and this is costly in resource limited settings. In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and is routinely done using either human or sheep plasma. This study evaluated Mannitol salt agar and the deoxyri...

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