Oguzhan Gunduz

• Ph.D
• Professor (Assistant) at Marmara University

Novel therapeutic strategies are essential for enhancing efficacy and accelerating the treatment of diabetes mellitus. This investigation focused on incorporating empagliflozin into a composite of polylactic acid and polycaprolactone, resulting in the fabrication of drug-loaded fibrous patches (DFPs) for transdermal application, both by electrospin...

This study introduces novel chitosan (CS) and polyethylene oxide (PEO) copolymers reinforced with bacterial cellulose (BC) to fabricate nanofibers using the electrospinning method. SEM analysis confirmed uniform nanofiber formation, with CS/PEO/BC nanofibers (~240 nm) exhibiting a larger diameter than CS/PEO ones (~190 nm). FTIR spectroscopy confir...

Skin disorders are the fourth most common cause of chronic and non-fatal skin conditions, leading to a worldwide burden and having an impact on the global economy. Skin conditions significantly impact patients’ overall well-being, encompassing their physical and psychological health. In this study, we aimed to evaluate the fabrication of mechanical...

Periodontal diseases, if untreated, can cause gum recession and tooth root exposure, resulting in infection and irreversible damage. Traditional treatments using autologous grafts are painful and often result in postoperative complications. Scaffolds offer a less invasive alternative, promoting cell proliferation and healing without additional surg...

Earthquakes are one of the most destructive natural hazards that cause loss of life, loss of property and destruction of infrastructure when they interact with vulnerabilities in human systems. The health impacts of these events are not only limited to immediate trauma and injuries, but also affect both physical and mental health in the short and l...

Recently developed nanoparticles and nanofibers present new brain‐specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)‐loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for sublingual administration. SEM images showed produced drug‐loaded...

Due to the probiotic characteristics of Bacillus subtilis and Bacillus coagulans, bacterial debris not only strengthens the immune system but also qualifies as a merit for biomedical products like toothpaste and implants for Hydroxyapatite (HA) synthesis. Since synthetic HA has caused some disadvantages, including raising proinflammatory cytokines...

In this study, the effects of 5‐FU, and its derivate Carmofur (Car) on MCF‐7 and MDA‐MB‐231 cells, were examined using FTIR. Both drugs showed a similar response in the protein and nucleic acid region of MCF‐7 but opposite results in MDA‐MB‐231 cells. Under the influence of the drugs, the absorption intensity of the proteins decreased in MCF‐7, whi...

Tympanic membrane (TM) perforation is a serious ear discomfort that can cause hearing loss and make the middle ear vulnerable to infections. In this study, a unique TM patch is designed to mimic the structure of the natural eardrum for tissue engineering of TM perforations. Gelatin methacryloyl (GelMA)‐based TM patches are equipped with microneedle...

Encapsulating antiepileptic drugs (AEDs), including ethosuximide (Etho), into nanoparticles shows promise in treating epilepsy. Nanomedicine may be the most significant contributor to addressing this issue. It presents several advantages compared to traditional drug delivery methods and is currently a prominent area of focus in cancer research. Inc...

Wound dressing is an ancient technique for promoting healing, and modern technology has led to the development of advanced dressings that enhance patient care. Nanofiber-based wound dressings are a medical innovation with enhanced properties, including improved adhesion, reduced infection rates, and increased tissue regeneration. This article focus...

3D printing technology allows food to be shaped into unique and complex forms. Chocolate is frequently used in food printing due to its extrusion melting capacity and attractiveness. In this study, chocolates enriched with ω-3 were produced with 3D printing technology. By using electrospraying, ω-3 was encapsulated within sodium alginate (SA) micro...

This study examined producing three-dimensional printed CS scaffolds coated with rifampicin (RIF)-loaded poly(lactic acid) (PLA) microparticles to treat hidradenitis suppurativa lesions. The morphology of the particle-coated scaffolds was examined; it was observed that the particles obtained were monodisperse and of an ideal size for the intended a...

Introduction: Microneedles (MNs) are miniaturized, painless, and minimally invasive platforms that have attracted significant attention over recent decades across multiple fields, such as drug delivery, disease monitoring, disease diagnosis, and cosmetics. Several manufacturing methods have been employed to create MNs; however, these approaches co...

GelMA hydrogels are prominent in biomedical applications due to their innate extracellular matrix mimicking properties. They exhibit favorable properties for cell proliferation and formation of light-induced hydrophilic cross-linked structures. However, there is limited research on the effect of variations in the starting material (gelatin) on the...

New gelatin methacryloyl (GelMA)—strontium-doped nanosize hydroxyapatite (SrHA) composite hydrogel scaffolds were developed using UV photo-crosslinking and 3D printing for bone tissue regeneration, with the controlled delivery capacity of strontium (Sr). While Sr is an effective anti-osteoporotic agent with both anti-resorptive and anabolic propert...

The aim of this study is to provide an overview of the current state-of-the-art in the fabrication of bioceramic scaffolds for bone tissue engineering, with an emphasis on the use of three-dimensional (3D) technologies coupled with generative design principles. The field of modern medicine has witnessed remarkable advancements and continuous innova...

Chronic skin wounds pose a global clinical challenge, necessitating effective treatment strategies. This study explores the potential of 3D printed Poly Lactic Acid (PLA) scaffolds, enhanced with Whey Protein Concentrate (WPC) at varying concentrations (25, 35, and 50% wt), for wound healing applications. PLA’s biocompatibility, biodegradability, a...

This study aims to develop gelatin methacryloyl (GelMA)‐based symblepharon rings fortified with l‐ascorbic acid (lAA), aiming for controlled release of vitamins for the treatment of the ocular surface, corneal healing, and acceleration of epithelial growth, while concurrently preventing potential inflammation. The human tears contain abundant IAA,...

Although different fabrication methods and biomaterials are used in scaffold development, hydrogels and electrospun materials that provide the closest environment to the extracellular matrix have recently attracted considerable interest in tissue engineering applications. However, some of the limitations encountered in the application of these meth...

Biomaterials are materials intended to interact with biological systems for the purposes of treatment, enhancement, or replacement of biological functions defined as material used in medical devices. Biomaterials in tissue engineering have been studied many years, and their level of innovation has increased significantly recently. In the field of t...

Skin is part of the integumentary and excretory system, which helps protect the body against infections. The skin should be properly treated when it gets injured, which requires a long healing process. In this study, 15% (w/v) polylactic acid (PLA) and 1 and 2% (w/v) polymethylsilsesquioxane (PMSQ) scaffolds were fabricated using 3D printing techno...

This study investigated the solubility of poly(lactic acid) (PLA) in various solvents (to achieve an 8 % (w/v) polymer solution) and its impact on the morphology of electrospun PLA nanofibers. Several solvents, such as dichloromethane (DCM), chloroform (CHL), tetrahydrofuran (THF), acetone (AC), acetonitrile (ACTNR), dimethylformamide (DMF), dimeth...

In recent years, Additive Manufacturing (AM), commonly referred to as 3D printing, has garnered the attention of the scientific community due to its capacity to transform ordinary and traditional items into customized materials at an affordable cost through various AM processes. Antimicrobial/antibiofilm 3D printed materials are one of the most tre...

Tympanic membrane (TM) perforations, primarily induced by middle ear infections, the introduction of foreign objects into the ear, and acoustic trauma, lead to hearing abnormalities and ear infections. We describe the design and fabrication of a novel composite patch containing photocrosslinkable gelatin methacryloyl (GelMA) and keratin methacryloy...

In recent years, Additive Manufacturing (AM), commonly referred to as 3D printing, has garnered the attention of the scientific community due to its capacity to transform ordinary and traditional items into customized materials at an affordable cost, through various AM processes. Antimicrobial/antibiofilm 3D printed materials are one of the most tr...

In this study, novel fibers were designed based on ethylcellulose (EC), loaded with different concentrations of gallic acid (GA) using the electrospinning technique, in order to investigate the potential of these materials as wound dressings. The chemical structure and morphology, along with the antimicrobial and biocompatibility tests of the EC_GA...

Silk has been consistently popular throughout human history due to its enigmatic properties. Today, it continues to be widely utilized as a polymer, having first been introduced to the textile industry. Furthermore, the health sector has also integrated silk. The Bombyx mori silk fibroin (SF) holds the record for being the most sustainable, functio...

This study is focused on fabricating tissue‐engineered electrospun nanofibers that contain polycaprolactone (PCL), graphene oxide (GO), and collagen (COL) to get an alternative treatment for cardiac injuries. GO (1.5 wt%) is used to support the contraction‐elongation of cardiomyocytes by improving electrical stimulation. The COL (1, 3, and 5 wt%) i...

Epilepsy is a medical condition that causes seizures and impairs the mental and physical activities of patients. Unfortunately, over one-third of patients do not receive adequate relief from oral Antiepileptic Drugs (AEDs) and continue to experience seizures. In addition to that, long term usage of Antiepileptic Drugs can cause a range of side effe...

3D-printed scaffolds can better mimic the function of human skin, both biologically and mechanically. Within the scope of this study, the effect of the addition of different amounts (10, 15, 20 mg) of gentamicin sulfate (GS) to a 10 mL solution of natural and synthetic polymers is investigated. Sodium alginate (SA), gelatin-methacryloyl (GelMA), an...

The application of biphasic calcium phosphate (BCP) in tissue engineering and regenerative medicine has been widely explored to possess its multi-functions extensively documented. The present study attempts to synthesize a new type of BCP nanoparticles with good cytocompatibility and antibacterial properties via imposing modifications in their stru...

Diabetic wounds are one of the most challenging clinical conditions in diabetes, necessitating the development of new treatments to foster healing and prevent microbial contamination. In this study, polyvinyl alcohol (PVA) was used as a matrix polymer, and amoxicillin (AMX) and salicylic acid (SA) were selected as bioactive compounds with antimicro...

Bioceramics commonly employed materials for the restoration, replacement and recovery of unhealthy and impaired pieces of the muscle and skeletal system, as well as periodontal anomalies. According to the host tissue interactions, bioceramics can be graded as nearly bioinert, bioactive, and bioresorbable. Most of the clinical applications of biocer...

Bone tissue engineering is based on a comprehensive understanding of bone structure, bone mechanics, and biology. In order to create nanostructured hydroxyapatite powders with customized properties, many synthesis strategies such as wet chemical precipitation, sol-gel, hydrothermal, and biomimetic approaches have been intensively researched through...

In this study, two-layer poly(vinyl alcohol)/gelatin (PVA/GEL) nanofiber patches containing cinnamaldehyde (CA) in the first layer and gentamicin (GEN) in the second layer were produced by the electrospinning method. The morphology, chemical structures, and thermal temperatures of the produced pure (PVA/GEL), CA-loaded (PVA/GEL/CA), GEN-loaded (PVA...

The electrospinning technique enables the convenient fabrication of non-woven fibrous materials with excellent properties such as fine diameters, a large surface area per unit mass, high porosity, high gas permeability, and small pore sizes. Nanofiber applications in healthcare systems continue to be popular especially in recent years and are integ...

The medicinal plant of Styrax liquidus (ST) (sweet gum balsam) which extracted from Liquidambar orientalis Mill tree, was loaded into the 3D printed polylactic acid (PLA)/chitosan (CS) based 3D printed scaffolds to investigate its wound healing and closure effect, in this study. The morphological and chemical properties of the ST loaded 3D printed...

Hydroxyapatite (HA) promotes excellent bone regeneration in bone-tissue engineering, due to its similarity to bone mineral and its ability to connect to living tissues. These factors promote the osteointegration process. This process can be enhanced by the presence of electrical charges, stored in the HA. Furthermore, several ions can be added to t...

This work evaluates the effects of manganese (Mn) doping on the morpho-structural features, mechanical performance, and in vitro biological response of beta-tricalcium phosphate (β-TCP) derived bioceramics for bone tissue engineering applications. Five different Mn doping levels (i.e., 0.01%, 0.05%, 0.1%, 0.5%, and 1 wt.%) were investigated, with t...

Skin is a protective barrier that can protect against environmental influences and renew itself. However, in some cases, this regenerative property is lost, and this causes delays in wound healing. Wound healing is a complex and long‐lasting phase. Any bacterial infection during the wound healing process delays wound healing. The therapeutic effica...

Microneedles (MNs) are micrometer-sized arrays that can penetrate the skin in a minimally invasive manner; these devices offer tremendous potential for the transdermal delivery of therapeutic molecules. Although there are many conventional techniques for manufacturing MNs, most of them are complicated and can only fabricate MNs with specific geomet...

Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual‐drug loaded dual‐layered...

Skeletal muscle tissue engineering presents a promising avenue to address the limitations pertaining to the regenerative potential of stem cells in case of injury or damage. The objective of this research was to evaluate the effects of utilizing novel microfibrous scaffolds, containing the compound quercetin (Q), on skeletal muscle regeneration. Mo...

In this study, a dental membrane scaffold was fabricated using a 3D printing technique, and the antimicrobial effect of pomegranate seed and peel extract were investigated. For the production of the dental membrane scaffold, a combination of polyvinyl alcohol, starch, and pomegranate seed and peel extracts was used. The aim of the scaffold was to c...

In this study, polyvinyl alcohol (PVA), gelatin (GEL) and gentamicin (GEN) were used and 13PVA/0.5GEL/GEN nanofibers were fabricated with the electrospinning method. These nanofibers were crosslinked chemically with glutaraldehyde (GA) at different time intervals (2, 3, 4, 5, 5.5 and 6 h) to observe the crosslinking effect on the properties of the...

Empagliflozin (EM) was successfully loaded in polycaprolactone/poly (L-lactic acid)/polymethyl methacrylate (PCL/PLA/PMMA) fibers. In the rat β-cell line (BRIN-BD11), the insulin expression ratio of pancreatic β-cells was stimulated at high and low glucose by culturing with tri-layer EM-loaded fiber (EMF) for 48 h. The expression ratios of glucokin...

Marine pollution has increased in recent decades, largely due to the proliferation of seafood processing plants and the improper disposal of their associated waste streams. The waste streams consist mainly of shells that are composed of chitin, which is the most abundant aminopolysaccharide biopolymer in nature. Recognizing the value of chitin, the...

The present study proposes two unique systems using free radical-induced grafting reactions to combine Ag, chitosan (CS) and gallic acid (GA) into a single particulate nanostructure. GA-grafted-CS (GA-g-CS) was used to reduce Ag+ to Ag0, and producing Ag-GA-g-CSNPs (hybrid NPs I). Also, GA was grafted into CS-AgNPs, to form GA-g-CS AgNPs (hybrid NP...

In this chapter, first, a general overview of biobased materials and their usage in the biomedical field is provided. Then, the relations between nerve regeneration and functionalized cell-ECM interactions with biobased materials are mentioned to highlight the cellular structures. The cellular effect mechanism of biobased materials in nerve regener...

In the present study, the effect of different ratios of GelMA concentration has been exhibited for wound dressing implementation by the electrospinning method using a new polymer combination of Gelatin methacrylate (GelMA)/Polycaprolactone (PCL)/Chitosan (CS). The nanofiber composites were fabricated due to their biocompatible, biodegradable, impro...

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by impaired insulin secretion, sensitivity, and hyperglycemia. Diabetic wounds are one of the significant complications of T2DM owing to its difficulty in normal healing, resulting in chronic wounds. In the present work, PCL/PVA, PCL/PVA/PCL, and metformin-loaded, PCL/PVA-Met and PC...

Bioceramics are a fast-growing materials group, which are widely used in orthopedics, maxillofacial, dental, and reconstructive surgeries. They are produced using raw materials either from synthetic or natural sources. As naturally originated resources, the bones of sheep and cows are used after converting to calcium phosphates. Human-originated so...

Aim: The purpose of this study was to design a sensor that could monitor the skin-cast contact surface pressure (SCCSP) of a limb under a cast and inform the user via a mobile application when the pressure increases. Methods: In this experimental study, an infant sphygmomanometer cuff was initially placed on the forearm of 10 volunteers. A press...