Ogbuagu Ugorji Udensi

• Ph.D
• Professor (Associate) at University of Calabar

Aims: This study was carried out to evaluate the evolutionary relationship of a family of cold shock proteins (CSP) in ten mammalian species using bioinformatics tools and soft wares such as Genbank, FASTA, BLAST and MEGA 5. Sample: Twenty protein sequences of both RBM3 and CIRP proteins of some selected mammalian species were downloaded from NCBI...

This study was aimed at using bioinformatics tools to characterize the Lipid Transfer Protein 1 gene in some selected accessions with special reference to Benny seed ( Sesamum indicum) Lipid Transfer Protein 1 sequence as a query sequence. Nucleotide and amino acid sequences of 30 accessions were retrieved from NCBI database and analyzed for homolo...

Pigeon pea Cajanus cajan (L.) Millsp. and cowpea Vigna unguiculata (L.) Walp. are two of the important seed legumes of Africa and are relatively more drought-tolerant than many other legumes. Nigeria is the largest producer of cowpea in Africa, while pigeon pea is yet to be commercially cultivated in Nigeria. The genomic information on cowpea is kn...

Antimicrobial resistance to typical antibiotics is a significant threat to human health worldwide, and its rapid progression has raised serious concerns in the treatment of infectious diseases. The cost of commercialising a new antibiotic is high relative to a low return on investment. Medicinal plants are used for the treatment of infections in ma...

Since the advent of the current pandemic of corona virus-induced disease in December 2019 to date, the search for knowledge has specifically been focused on finding prophylactic and therapeutic solutions for COVID-19 which has murdered approximately 1,073,675 globally as of October 11, 2020 thus intensifying the global search for a cure to save hum...

Effects of mutagens on the chromosomes of crop plants are a good indicator to deciphering the extent to which they can be utilized for crop improvement. The aim of this research work was to evaluate the cyto-sensitivity of two landraces of pigeon pea [Cajanus cajan (L.) Millsp] to amiprophos methyl (APM). The seeds from the two varieties were soake...

Cajanus cajan (L.) Millsp. is an important legume crop that grows in semiarid tropic continents such as Asia, South America and Africa. Sub-Saharan African countries, particularly Nigeria, have to achieve food, crop and nutrition security, because the population faces hunger and malnutrition. Thus, to mitigate these challenges, developing and impro...

This study was aimed at inducing mutation on three cultivars of Capsicum annuum L. to source for new and favourable genetic variations that could be exploited for better agronomic traits. The mutation was induced by exposing the seeds of Shombo, Tatase and Nsukka yellow pepper to varied dosages (0, 50, 100, 150 and 200 Gy from 60Co source) of gamma...

INTRODUCTION Most recently, there has been an upsurge of DNA and protein sequence data deposited in genbank or databases that are subjected to analysis, which is made possible through the utilization of bioinformatics tools. However, the accuracy and informativeness of these sequences often analyzed depend on the suitability of the bioinformatics S...

INTRODUCTION Most recently, there has been an upsurge of DNA and protein sequence data deposited in genbank or databases that are subjected to analysis, which is made possible through the utilization of bioinformatics tools. However, the accuracy and informativeness of these sequences often analyzed depend on the suitability of the bioinformatics S...

Background: Primary open-angle glaucoma is one of the commonest causes of blindness due to optic nerves damage because of elevated intraocular pressure and it is the commonest form of glaucoma. We determined the frequency of CYP1B1 gene mutations spectrum among families with open-angle glaucoma in Calabar. Methods: Ophthalmologists examined the rec...

Background: Primary open-angle glaucoma is one of the commonest causes of blindness due to optic nerves damage because of elevated intraocular pressure and it is the commonest form of glaucoma. We determined the frequency of CYP 1 B 1 gene mutations spectrum among families with open-angle glaucoma in Calabar. Methods: Ophthalmologists examined the...

Aims: This study was carried out to evaluate the evolutionary relationship of a family of cold shock proteins (CSP) in ten mammalian species using bioinformatics tools and soft wares such as Genbank, FASTA, BLAST and MEGA 5. Sample: Twenty protein sequences of both RBM3 and CIRP proteins of some selected mammalian species were downloaded from NCBI...

Genotyping of crop plants, especially pigeon pea is very strategic in the global quest for crops that will be suitable for the precarious climate change as it provides genotypes for introgression. 22 pigeon pea accessions obtained from IITA germplasm were evaluated for genetic diversity. Genomic DNA was extracted using SDS protocol from young leave...

Background: Breast cancer type 1 (BRCA1) gene also known as breast cancer type 1 susceptibility protein homologue plays important role in DNA double-strand break repair, homologous recombination, chromatin remodeling, cell cycle regulation and transcription regulation. Objective: Considering the importance of this protein, the present study was und...

Multivariate based genetic analysis of three genotypes of Nigerian local chicken (Gallus domesticus) was studied to assess morphological diversity. Forty (40) adult local chickens which comprised frizzle feather, naked neck and normal feather were obtained from two different locations; Obudu in Cross River State and Umudioka in Anambra State. The c...

Genetic diversity in germplasm is cardinal for breeders as it provides potential genotype (s) for breeding and improvement. Sixty C. gariepinus samples were obtained from the wild and cultured populations in Cross River, Nigeria. Twenty one morphometric traits were taken from each fish sample and were later prepared for proximate and mineral compos...

This study was designed to evaluate the effects of chlorpyrifos and cypermethrin, singly and in combination on reproductive physiology of male albino rats. Thirty six mature male rats were used for this study. Completely randomized design was used in a 3×3 factorial format. Meaning there were 3 treatments (chlorpyrifos, cypermethrin and chlorpyrifo...

Aspartame (ASP) consumption has been reported to implicate oxidative stress and antioxidant supplements from plant origin are one of the safest ways of averting their effect in the body. The current study was aimed at ascertaining the ability of aspartame (ASP) to induce oxidative stress in rats as well as assessing the potential of ginger (Zingibe...

This research was aimed at assessing the genetic diversity in cowpea using RAPD analysis. Twenty cowpea accessions were obtained from the Genetic Resource Unit (GRU) of International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and planted in the screening house of the same institute. The seeds from the 20 cowpea accessions were plante...

The utilization of molecular markers-assisted genetic diversity study is critical and imperative for a more robust selection and breeding programmes in aquaculture. Twenty C. gariepinus species were randomly selected, 5 each from four Rivers/locations-Okurikan (OKU), Lemna (LMN), UNICAL Fish Farm (UFF) and MCC cultured fish site, in Cross River Sta...

This study was aimed at assessing the reproductive activities of male albino rats treated with crude leaf extract of Bougainvillea spectabilis. The total of thirty sexually matured male albino rats of about eleven weeks, weighing between 120-180 g were divided into five groups (A, B, C, D and E) with 6 rats in each group. Rats in group A served as...

Thirty accessions of cowpea from Ebonyi, Enugu and Kogi States, Nigeria were used for the study. Seeds of the accessions were randomized in a plot measuring 50x50 meters for three growing seasons. Our results on yield and yield-related traits showed that three principal components were extracted, which contributed 82.23% of the total variability. I...

The current research was aimed at comparing the leaf extracts of two medicinal plants (Moringa oleifera and Azadiracta indica) for antioxidant and free radical scavenging potentials in different extracting solvents (absolute ethanol, 70 and 50% ethanol). Different in vitro assays such as total phenolic and flavonoid content, 2-2-diphenyl-1-picrylhy...

Objective: To assess the efficacy of Hibiscus sabdariffa (H. sabdariffa) and Telfairia occidentalis (T. occidentalis) extracts in the attenuation of CCl4-mediated oxidative stress. Methods: Seventy-two healthy matured male albino rats weighing between (120±20) g were divided into 6 groups (A-F) in a 2×6 factorial experiment using completely rand...

Creation of variability through induced mutation is cardinal in genotype selection. However, the stability of the mutants in the subsequent generations is of utmost importance for improvement. This current research was aimed at evaluating the performance of pigeon pea landraces following amiprophos methyl (APM) treatment. Thirty seeds each of two v...

Aim: This research was aimed at evaluating the antioxidant efficacy of fresh and dried fruits of Persea americana. Methods: Various in vitro asaays such as total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, total antioxidant capacity, reducing power and hydroxyl radical scavenging were used...

Herbal plants have been found to be effective in traditional medicine for healthcare. However, the full potentials of these natural resources are yet to be fully explored. This research was coined at investigating the effects of Tetracarpidium conophorum seeds on the hormone and sperm profile of male albino rats. Forty eight albino rats of about tw...

This study was designed to evaluate the effect of ethanolic stem bark extract of Nauclea latifolia, Cylicodiscus gabunensis and Araliopsis souyauxii; three plants used locally as anti-malarials, on haematology and serum enzyme levels in albino rats. Employing a 3x3 factorial arrangement, thirty six male albino rats were randomly assigned into nine...

In this study, stem bark extracts of Cylicodiscus gabunensis, Nauclea latifolia and Araliopsis soyauxii were investigated for possible adverse effects on male reproductive organs and sex hormones of male albino rats of about eleven weeks weighing between 120-180g. The total of twenty eight rats were divided into seven groups (A, B, C, D, E, F and G...

The need to optimize flow cytometric analysis for the determination of ploidy level is a worthwhile venture to precisely know at what concentration of a mutagen and at what time of exposure polyploidy could be induced. Flow cytometry was used to determine the polyploidy inducing-capacity of colchicine in pigeon pea (Cajanus cajan (L.) Mill sp). See...

The seeds of two varieties of pigeon pea [Cajanus cajan (L.) Mill], brown " Fiofio " and white " Fiofio " , were treated with 0, 4, 6 and 8 ppm amiprophos methyl (APM) for 48 h, or exposed to γ-irradiation at 0, 200, 400, 600 Gy (Gray) from 60 Co source. There was significant (P < 0.05) effect of the mutagenic treatments on all the growth and yield...

Giving the high adaptability, heritability, genetic variability and nutritive values reported in the indigenous landraces of legumes, combined efforts should be geared towards their improvement. The present study aimed at evaluating the sensitivity of "Fiofio" Cajanus cajan (L.) Huth and "Olaudi" Vigna unguiculata (L.) Walp to gamma irradiation usi...

Demand for new chemotherapies against malaria is increasing as Plasmodium, the causative organism of the disease, has shown a high degree of resistance against most standard anti-malarial drugs. One of the few highly effective compounds is artemisinin, the major sesquiterpene for the production of artemisinin-based combination therapies (ACTs). Thi...

A four-day 4 suppressive schizontocidal test for anti malarial activity against Swiss albino mice infected with chloroquine sensitive strain (NK-65) of Plasmodium berghei was used to monitor in vivo response to different modes of preparing herbal remedies using the stem and bark of Azadarachta indica Juss. Four recognized modes of preparing anti ma...

Aims: It is utmost desire for plant breeders to know the extent of relationship between yield and its various components, which will inevitably facilitate selection of desirable characteristics. This research focused on computing the correlation coefficients and path coefficients of yield and its contributing traits in pigeon pea landraces Cajanus...

Aim: Effects of gamma irradiation on amino acid profile, proximate and anti-nutritional compositions of selected landraces of pulses were investigated. Methods: Seeds of Brown “Fiofio”, White “Fiofio” ( Cajanus cajan ) and “Olaudi” ( Vigna unguiculata ) were exposed to gamma irradiation. Each variety was divided into five groups of fifty grams weig...

Establishing the extent of relationship, identifying the cause and measuring the relative importance of such associations to yield is very crucial for varietal selection, breeding and subsequent improvement of crops, especially cowpea landraces. This research x-rays correlation coefficients and path coefficients of yield and yield-influeneing trait...

A major constraint in the improvement of 'Egusi' melon lies in the fact that attempts at transferring desirable genes into the plant from wild relatives are limited by interspecific hybridization barriers. Therefore, antimitotic agents were used as a means of introducing genetic variability for possible incorporation into breeding programmes of the...

Effect of soybean (Glycine max) on serum level of some sex hormones: testosterone, follicle stimulating hormone (FSH), luteinizing hormone/interstitial cell stimulating hormone (LH/ICSH), estradiol and prolactin in male rats was investigated. Twenty male albino rats of 12 weeks old with similar body weights were assigned to four groups of 5 rats ea...

This study investigated the nutritive value of locally grown pulses' landraces (White "Fiofio", Brown "Fiofio", [Cajanus cajan (L.) Millsp] "Olaudi" "Akidi" and IT88D-867-11) [Vigna unguiculata (L) Walp] as indices for conservation and improvement. Proximate, anti-nutritional and mineral elements were evaluated. Data analyses were done using analys...

The negligence of breeders and farmers to explore and exploit landraces of pulses is worrisome and urgent measures needed to be set in motion to forestall major future crisis, taking into cognizance the high adaptability and nutritive values accredited to them. This study focused on the estimation of genetic variability and heritability of desirabl...

This study was aimed at qualitative evaluation of the ethanol seed, leaf and pulp extracts of C. papaya for bioactive compounds and also to investigate their effect on the haematology in male albino rats. A 3 x 4 factorial experimental layout using randomized complete design was adopted. Results show that the phytochemicals found in seed, leaf and...

The effects of aqueous leaf extract of neem on serum testosterone, follicle stimulating hormone (FSH), luteinizing hormone/interstitial cell stimulating hormone (LH/ICSH), estrogen and prolactin levels in male albino rats were studied. Rats treated with 50, 100 and 150 mg/kg body weight of neem extract intraperitoneally daily for 15 days showed ver...

Pumpkin (Cucurbita ficifolia L.) is a climbing or creeping monoecious annual plant grown in Nigeria for its stem, leaves, fruits and seeds, which are rich sources of calcium, potassium and vitamins (niacin). The effects of growth regulator (IAA and BAP) applications [ 0 (distilled water, control), 50, 100 and 150 mg l-1] on the growth characteristi...

Correlation and genetic variability in cassava (Manihot esculenta Crantz) was studied using 6 varieties, viz: ADP 3 (4), ADP 3(5), ADP 4(4), TMS 3055, TMS 419 and one local variety Panya, all obtained from the Cross River Agricultural Development Programme, Calabar, Nigeria. The research was carried out at the Biological Sciences Experimental Farm,...