O. V. Dorogina

• Central Siberian Botanical Garden, Russian Academy of Sciences

Miscanthus sinensis Andersson (Poaceae) grows in monsoon climate. For this reason, when cultured under the conditions of a short growing season of Western Siberia, full-fledged seeds do not have time to form. We have studied a large number of specimens of this species from Primorsky Krai in the collection of the Central Siberian Botanical Garden, S...

The breeding of remontant rose cultivars that are resistant to diseases and adverse conditions, with high decorative value and continuous flowering is the most important task during work with the gene pool of garden roses. Currently, intercultivar hybridization within a single garden group has largely outlived its usefulness. It is necessary to bre...

A hybridological study of biotypes of species close to Elymus caninus : E. prokudinii , E. viridiglumis , E. goloskokovii , as well as a number of morphologically deviant biotypes in Russia and Kazakhstan, was carried out. The objectives were to study the levels of reproductive relationships and the degree of integration of the species E. goloskoko...

Background. Rhaponticum carthamoides (Willd.) Iljin (Asteraceae) is a rare species for the Altai Republic (AR). The purpose of this study was to characterize the genetic polymorphism of Rhaponticum carthamoides at the inter- and intrapopulation level in a comparative analysis for subsequent selection of seed samples from the genetically most hetero...

The concept of “Introduction” is ambiguous. It is associated with a long period of human introduction activity. There are six stages of introduction development: from 8000 years BC (intuitive stage) to the present. For a long time, the practical development of the introduction was significantly ahead of the theoretical one. In 1972, the adopted def...

The generic complex Miscanthus Anderss. (Poaceae) is a unique example among herbaceous plants characterized by high values of growth of aboveground vegetative mass and practical use as a valuable source of alternative energy. Miscanthus is one of the most efficient solar energy accumulators, and since phytomeliorative use implies the cultivation of...

Based on sequence data of the nuclear ETS markers of 20 species, the relationships of sections Hedysarum and Multicaulia were assessed. Phylogenetic analysis was performed on the complete sequences using the Neighbor-Joining methods. The nuclear marker ETS is investigated for the first time in the most species excluding H. hedysaroides, H. inundatu...

We studied the nucleotide variability in two morphologically similar and taxonomically close endemic legume species, Hedysarum austrosibiricum and H. consanguineum. To estimate the level of genetic divergence between the species, four DNA loci were sequenced and analysed: ITS region of nuclear ribosomal DNA and three non-coding regions of chloropla...

Background. Trypsin-inhibiting activity (TIA) is one of the factors of plant immunity. This factor provides the presence of general non-specific systemic resistance. Representatives of the genus Hedysarum L. are promising medicinal and high-protein fodder plants with high TIA in the leaves. High TIA values in the aboveground part of plants lead to...

Identifying among varieties and natural forms of roses were carried out using ISSR thechnics to determine kinship. It was studied 6 foreign varieties, 16 varieties bred in Nikitsky Botanical Garden, as well as 2 natural forms (Rosa canina and Rosa chinensis). Despite some samples breaking into three clades, many of them don’t belong to any of these...

Several species of the genus Miscanthus Anderss. (elephant grass) characterized by a high rate of growth of the aboveground vegetative mass are currently in the focus of attention due to their high practical application as a source of bioethanol and cellulose. The main goals of this study were: (1) molecular genetic identification and (2) histochem...

Here we provide a scientific justification and experimental support for the choice of easily renewable cellulosic feedstock Miscanthus sacchariflorus (Maxim.) Hack. in order to obtain high-quality nutrient broths therefrom for bacterial cellulose biosynthesis. The plant life-forms promising for breeding were screened under introduction conditions a...

A significant length polymorphism (309–735 bp) and nucleotide variability were detected by the study of the intergenic spacer trnL-trnF of chloroplast DNA in Hedysarum species belonging to sections Hedysarum and Multicaulia (family Fabaceae). Among nineteen Hedysarum species analyzed, the region trnL-trnF was not investigated before for thirteen sp...

In the meantime, search for environmentally friendly renewable energy sources alternative to fossil fuel has been driven by energy security challenges including limited availability of fuel and energy price fluctuations. Therefore herbal perennial grasses with their rapid growth and prominent biomass yield increasingly make it a favorite choice as...

Morphostructural features of aerial parts of shoots have been studied in endemic Hedysarum austrosibiricum B. Fedtsch growing at the altitude range of 1200–2350 m in the mountain areas of the Altai Republic (AR) and the Republic of Khakassia (RK). A complex of morphological traits adaptive to mountain conditions has been revealed in the studied spe...

A multidisciplinary study, engaging morphological, carpological and molecular data, has been performed to investigate the genetic relatedness and taxonomic boundaries of the close species Hedysarum gmelinii, H. setigerum and H. chaiyrakanicum (Fabaceae) with overlapped distribution areas in southern Siberia. The diagnostic features of these legume...

Overgrazing and mining affect vegetation, particularly in mountains. At times, it goes to such an extent that the plant species become vulnerable and slowly extinct from its habitat. Such endemic species need to be protected. One such endemic species Hedysarum chaiyrakanicum Kurbatsky, a vulnerable steppe vegetation of Tuva Republic, Russia was eva...

The morphological structure of the aboveground part of the sprouts of the Astragalus mongholicus Bunge s.l. plants, which grow in Gorny Altai and Buryatiya and Irkutsk regions, was studied. The complex of adaptive morphological traits of this species has been revealed. The dependence of the traits on the confined-ness of the plants to the different...

In the article - quantitative determination of total flavonoids in the grass plants of clover growing in the Kemerovo region. This study flavonoid content in plants is essential for solving a number of problems chemotaxonomic direction. The studied species of clover differ in quantitative content of total flavonoids. Maximum mass concentration (%)...

Cloning of the Arabidopsis thaliana genomic DNA fragment presumably corresponding to the promoter region of the ornithine-delta-aminotransferase (OAT) gene is reported. The reporter-gene construct, containing the Escherichia coli beta-glucouronidase gene under control of the OAT gene promoter was generated. The Nicotian tabacum SR1 transformants ca...

Cloning of the Arabidopsis thaliana genomic DNA fragment presumably corresponding to the promoter region of the ornithine-delta-aminotransferase (OAT) gene is reported. The reporter-gene construct, containing the Escherichia coli beta-glucouronidase gene under control of the OAT gene promoter was generated. The Nicotian tabacum SR1 transformants ca...

Lines of the spring common wheat Omskaya 37 were studied, and wheat-rye translocations were determined by genomic in situ hybridization 1RS. 1BL. This translocation was transferred to Omskaya 37 from the wheat cultivar Kavkaz. An assessment of crop productivity, grain quality, and resistance to leaf pathogens in these lines was carried out compared...

Relationships between the range of variation of electrophoretic profiles of seed polypeptides and ecological-phytocenotic conditions of the habitats of thirteen populations of Hedysarum theinum Krasnob. (tea sweetvetch) were studied. The variability of the seed polypeptide profiles were lower in H. theinum populations from the alpine zone than in t...

Viable lily regenerants are produced by interspecific hybridization and in vitro culturing of hybrid embryos. The isozyme analysis method is used for studying the hybrids obtained. The possibility of identifying them at early stages of ontogeny in an in vitro culture is shown.

Legumes are one of the cheapest protein sources. But most of them contains inhibitors tripsin and needs in long processing. Micronization and cooking of legumes only partly destructs the protease inhibitors. The using this products as fast processing food is unwished.