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The present study investigates the anatomical and morphological characteristics of four Taenitis species sampled from several localities in Peninsular Malaysia, namely Taenitis blechnoides, T. interrupta, T. dimorpha and Taenitis sp. The morphological and anatomical characters were observered with compound and dissecting microscopes, and sliding mi...
Cyathea latebrosa is one of the lowland tree fern species found in Peninsular Malaysia. This fern species is highly demanded in ornamental landscaping. The in vitro cultures are an important tool for propagation which may contribute toward the reduction of over-exploitation. To overcome these problems, an effective spore surface disinfection protoc...
A new record of the fern genus Cornopteris Nakai from Peninsular Malaysia is presented. The species, C. opaca (D. Don) Tagawa was encountered during recent fieldwork in Mount Berinchang, Cameron Highlands. Previously, it has only been recorded from Mount Kinabalu in Sabah, East Malaysia. A description, photographs, and a distribution map of known o...
Ferns and lycophytes were opportunistically collected along the major trail at Gunung Basor Forest Reserve near Pergau Dam, Kelantan. Samples were curated, identified and deposited at Universiti Kebangsaan Malaysia Herbarium (UKMB). From the sampling area, a total of 94 taxa of ferns and lycophytes in 55 genera and 29 families were identified. This...
A total of 57 taxa in 37 genera and 24 families of ferns and lycophyte has been recorded in Pulau Pangkor and the surrounding areas. These number of taxa represents 8.8% of the total 647 taxa of ferns and lycophytes, which have been recorded for Peninsular Malaysia. Polypodiaceae is the largest family with nine species followed by Gleicheniaceae, A...
All my Selaginella got contamination after sterilization phase. I already
try to change method for sterile it such :-
1. soak them in fungicide + tween 20
2. rinse with dh2O (3times)
3. dip in 70% ethanol (30second)
4. soak in sodium hypochlorite/clorox (20%-60% concentration) / 0.1% mercury chloride(HgCl2) for 5-10 min.
5. rinse with dH2O (3-5 times)
6. dry it on filter paper
7. put in media
Any suggestion, what should i do to improve it from contamination?
I already try to sterile my fern for in vitro spore culture by:-
1. wash under running tap water
2. then shake with fungicide + distill water
3. rinse with distill water(3 times)
4. dip in 1 min 70% ethanol
5. dip in difference concentration of sodium hypochlorite (if higher concentration the it cannot grow but lower concentration cause fungus)
6. rinse with distill water (3 times)