Nuno MendonçaUniversity of Coimbra | UC · Institute for Interdisciplinary Research
Nuno Mendonça
Ph.D. MBA
About
48
Publications
3,921
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856
Citations
Citations since 2017
Introduction
Higher Education and Researcher professional, with a Ph.D. in Microbiology and an Executives MBA, highly engage in social development:
- High experienced in scientific writing (papers published in international peer review journals, projects financed by the European Society of Clinical Microbiology and Infectious Diseases and from Portuguese Foundation for Science and Technology);
- Entrepreneur in a bioinformatics startup project aiming to reduce antibiotic consumption and resistance;
Additional affiliations
January 2014 - present
September 2010 - January 2013
April 2009 - March 2015
Universidade de Coimbra
Position
- PostDoc Position
Education
September 2011 - June 2013
September 2006 - March 2009
October 1996 - July 2002
Publications
Publications (48)
Salmonella enterica is a foodborne pathogen showing increasing multidrug resistance (MDR). We characterized the antimicrobial resistance (AMR) genotype using microarrays in a panel of 105 nontyphoidal S. enterica isolated from food animals and foodstuff. Nineteen isolates were chosen on the basis of their MDR and virulence for determination of heav...
Background:
Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported.
Objectives:
This study screened for presence of transferable resistanc...
Gut inflammation can fuel horizontal gene transfer between pathogens and other gut bacteria (1). This study
characterized the molecular mechanisms of virulence, antimicrobial and heavy metal resistance (AMR/HMR) of
106 Salmonella enterica strains of food origin. A high throughput virulence genes microarray was designed, and
whole genome sequencing...
The presence of antimicrobial resistance and virulence factors of 174 Escherichia coli strains isolated from healthy Portuguese Gallus gallus was evaluated. Resistance profiles were determined against 33 antimicrobials by microbroth dilution. Resistance was prevalent for tetracycline (70%) and ampicillin (63%). Extended-spectrum beta-lactamase (ESB...
ABSTRACT
Introduction: Salmonella infection is a major foodborne illness worldwide.
Salmonella Pathogenicity Islands (SPI) are necessary for invasion and infection
of host cells. S. Typhi produces a cytolethal distending toxin (CDT: CdtB, the
active form, PltA and PltB). CdtB arrests cell cycle induces apoptosis and extends
persistence of pathogeni...
Salmonella enterica is a zoonotic foodborne pathogen that causes acute gastroenteritis in humans. We assessed the virulence potential of one-hundred and six Salmonella strains isolated from food animals and products. A high through-put virulence genes microarray demonstrated Salmonella Pathogenicity Islands (SPI) and adherence genes were highly con...
This study aimed to characterize the relationship between pathogenicity islands (PAIs), single virulence genes and resistance among uropathogenic Escherichia coli, evaluating the resistance plasmid carriage fitness cost related to PAIs. For 65 urinary E. coli, antimicrobial susceptibility and extended-spectrum β-lactamase production were determined...
INTRODUCTION Non-typhoid Salmonella (NTS) is a common zoonotic foodborne enteropathogen worldwide both in developing and industrialized countries. Salmonella cause each year 93.8 million cases of acute gastroenteritis worldwide. 1 In Europe, salmonellosis is the second most common zoonosis, affecting over 100,000 individuals each year. 2 Salmonella...
Escherichia coli is simultaneously an indicator of water contamination and a human pathogen. This study aimed to characterize the virulence and resistance of E. coli from municipal and hospital wastewater treatment plants (WWTPs) in central Portugal. From a total of 193 isolates showing reduced susceptibility to cefotaxime and/or nalidixic acid, 20...
Abstract As Salmonella is a common foodborne pathogen, the present study aimed to determine the distribution of Salmonella enterica serotypes isolated during 2011-2012 from poultry, swine, cattle, and processed food in Portugal, and to characterize the antimicrobial susceptibility and the extended-spectrum β-lactamases (ESBLs). Results were also co...
The objective was to characterize virulence markers and β-lactam resistance in Klebsiella pneumoniae isolates from renal transplant patients and to evaluate their genetic relatedness. Two main genetic lineages were detected: one carried blaCTX-M-15 not associated to IncFIIA plasmid replicon, which was found on the other lineage not expressing CTX-M...
Introduction:
The main objective of the study was the molecular characterization of extended spectrum β-lactamases (ESBL) in Escherichia coli isolates collected from human and bovine samples in Oyo state, Nigeria.
Methodology:
Between August 2010-2011, 114 E. coli isolates were collected from hospitals (n = 57) and bovine (n = 57). PCR and seque...
INTRODUCTION S. enterica is a pathogen with high expression in the world, both in developing and industrialized countries. Gastroenteritis infections are mostly self-limiting, however, a serious and potentially fatal problem requires antimicrobial treatment. 1 Significant bacterial virulence factors are necessary to adhere, invade and replicate ins...
INTRODUCTION Salmonella is common zoonotic foodborne enteropathogenic bacteria, causing each year 1.3 billion cases of acute gastroenteritis. Salmonella have several virulence factors that help in host cells invasion and multiplication. Due to the self-limiting nature of illness, typical cases of acute Salmonella diarrhea are not usually treated wi...
Escherichia coli ST131 is an important cause of multidrug-resistant infections. Thus, the aim of this study was to evaluate the concomitant presence of resistance plasmids and pathogenicity islands (PAIs) in ST131 E. coli. From 97 extra-intestinal E. coli characterized for antimicrobial susceptibility and extended-spectrum β-lactamase production, 1...
SUMMARY Twelve non-replicate Acinetobacter baumannii isolates from five European hospitals, Kuwait, and the US military healthcare system collected between 1980 and 2005 revealed a new clone, CC32. These included representative isolates of outbreaks/cross-infections. Antimicrobial susceptibility and carbapenem-resistant genetic traits varied. The w...
Objectives: The main goal of the study was to determine antibiotic susceptibility and the prevalence of extended-spectrum beta-lactamase (ESBL) producing Proteus spp. isolated from clinical samples from southwestern Nigeria hospitals. Methods: Proteus spp. isolates (n=120) were collected from diverse clinical specimens in hospitals from four states...
This work aimed to investigate the association of the carriage of plasmid-mediated quinolone resistance (PMQR) genes, the virulence potential encoded in pathogenicity islands (PAIs) and the phylogenetic background in Escherichia coli strains isolated from waters of diverse origin. Antimicrobial susceptibilities were determined by the disc diffusion...
Escherichia coli represents a major cause of morbidity and mortality worldwide. The treatment of E. coli infections is now threatened by the emergence of antimicrobial resistance. The dissemination of resistance is associated with genetic mobile elements, such as plasmids, that may also carry virulence determinants. A proficient pathogen should be...
Extraintestinal pathogenic Escherichia coli (ExPEC) cause infections such as urinary tract infections, septicemia and meningitis.…
We investigated the diversity of nucleotide sequences of 297 SHV-encoding genes, based on nucleotide synonymous mutations and the presence or absence of the nonsynonymous mutation T92A. The analysis of this diversity allowed us to develop and propose a classification to differentiate the nucleotide sequence variants of the bla(SHV) genes.
All 187 Klebsiella pneumoniae isolated over six consecutive months of 1999 in 17 Portuguese health institutions were studied: 89% were resistant to ampicillin, 31% to trimethoprim/sulfamethoxazole, 17% to aminoglycosides and 3% to fluoroquinolones; 16% were multidrug-resistant and 14% expressed an extended-spectrum beta-lactamase (ESBL) phenotype c...
Antimicrobial resistance is a growing problem worldwide, with extended-spectrum β-lactamase (ESBL)-producing organisms remaining an important cause of cephalosporin therapy failure. The main purpose of the work presented in this dissertation was to search for the molecular diversity of Ambler class A β-lactamase encoding genes in clinical Klebsiell...
Of the 308 clinical Klebsiella pneumoniae strains collected in 21 Portuguese health institutions, 11 encoded for LEN and 9 for OKP enzymes; of these, 15 were new enzymes. Ninety-one percent of LEN and all OKP producer strains were resistant to amoxicillin. We demonstrate that these beta-lactamase were highly diverse.
The new β-lactamase SHV-72 was isolated from clinical Klebsiella pneumoniae INSRA1229, which exhibited the unusual association of resistance to the amoxicillin-clavulanic acid combination (MIC, 64
μg/ml) and susceptibility to cephalosporins, aztreonam, and imipenem. SHV-72 (pI 7.6) harbored the three amino acid substitutions
Ile8Phe, Ala146Val, and...
Of the 181 unduplicated Escherichia coli strains isolated in nine different hospitals in three Portuguese regions, 119 were extended-spectrum β-lactamase (ESBL)-CTX-M
producers and were selected for phenotype and genotype characterization. CTX-M producer strains were prevalent among community-acquired
infections (56%), urinary tract infections (76%...
Sixteen strains of Escherichia coli isolated between January and June 2005 in a hospital in Algiers carry the ISEcp1 element and the TEM and either CTX-M-3 (n = 3) or CTX-M-15 (n = 13) β-lactamases. Fourteen of the isolates are multidrug resistant. Five isolates from the neonatal ward were indistinguishable
by pulsed-field gel electrophoresis.
Fifty-five isolates of Klebsiella pneumoniae were evaluated for extended-spectrum beta-lactamase (ESBL) detection and confirmation, using MIC testing by agar dilution, broth microdilution, and the ESBL E-Test (AB Biodisk, Solna, Sweden), according to reference laboratory criteria (RLC) and Clinical and Laboratory Standards Institute (CLSI) guidelin...
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (9...
Questions
Question (1)
Join us for 2014 edition of PostDoc Day at the University of Coimbra.
The event on Thursday morning is open to future, past and current Postdoctoral Researchers.
This year, postdocs will present work undertaken at their individual units, with an emphasis on the role of postdocs and potential avenues for collaboration and research networks.
Register now, and see you at the Faculty of Pharmacy on October 30.
Projects
Projects (2)
This work aims to gather information and to build a updated data base, covering the University of Coimbra (UC), studying the faculties and organic units, about the current situation of generation, recovery, reuse and destination of the different types of e-waste. Also, the project will seek to arrive at a set of recommendations, suggestions for improvement and concrete proposals related to the management of e-waste at the UC, using sustainable tools and circular economy approaches in order to minimize the impacts of this waste and increase the levels of waste. Through comparisons with other entities and case studies, it is therefore also intended to create, develop and validate guidelines for the promotions of sustainability.
A link service to minimize distances between University and Industry, as a clear commitment of the University of Coimbra. Potentiate funding through projects with enterprises, solving issues placedby these, is a natural consequence of the shift to a new paradigm: the University of Coimbra as innovation partner.
