Nuno M Guimaraes

Nuno M Guimaraes
University of Porto | UP · Laboratory for Process, Environmental and Energy Engineering

PhD

About

28
Publications
13,036
Reads
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414
Citations
Introduction
Nuno M Guimaraes currently works at the Laboratory for Process, Environmental and Energy Engineering, University of Porto. Nuno does research in Biotechnology, Microbiology and Molecular Biology. Their current project is 'Target delivery systems for nucleic acid mimics oligonucleotides for Helicobacter pylori treatment'.
Additional affiliations
May 2013 - June 2013
University of Southern Denmark
Position
  • PostDoc Position
January 2012 - present
University of Porto
Position
  • PostDoc Position
January 2007 - December 2010
University of Minho
Position
  • PhD Student

Publications

Publications (28)
Article
The transmission of the gastric pathogen Helicobacter pylori involves the oral route. Molecular techniques have allowed the detection of H. pylori DNA in samples of the oral cavity, although culture of H. pylori from these type of samples has been sporadic. Studies have tried to demonstrate the presence of H. pylori in adenotonsillar tissue, with c...
Article
Full-text available
The standard methods for accurate diagnosis of Helicobacter pylori infection consist in either culturing of the pathogen and/or concordant positive results obtained by histology and the rapid urease test or the 13C-urea breath test (UBT), as none of these diagnostic tests except bacterial culturing are 100% specific, fluorescence in situ hybridizat...
Article
Full-text available
Twenty-five years after the first successful cultivation and isolation of Helicobacter pylori, the scientific community is still struggling to understand the way(s) this bacterium is transmitted among the human population. Here, both epidemiologic and microbiologic evidence addressing this matter is reviewed and explored to conclude that most H. py...
Article
The rise of infectious diseases as a public health concern has necessitated the development of rapid and precise diagnostic methods. Imaging techniques like nuclear and optical imaging provide the ability to diagnose infectious diseases within the body, eliminating delays caused by sampling and pre-enrichments of clinical samples and offering spati...
Article
Full-text available
Antimicrobial resistance (AMR) is a growing concern because it causes microorganisms to develop resistance to drugs commonly used to treat infections. This results in increased difficulty in treating infections, leading to higher mortality rates and significant economic effects. Investing in new antimicrobial agents is, therefore, necessary to prev...
Article
Full-text available
Antisense oligonucleotides (ASOs) composed of nucleic acid mimics (NAMs) monomers are considered as potential novel therapeutic drugs against bacterial infections. However, bacterial envelopes are generally impermeable to naked oligonucleotides. Herein, liposomes loaded with NAMs-modified oligonucleotides (LipoNAMs) were evaluated to deliver ASOs i...
Article
Full-text available
Development of specific probes to study the in vivo spatial distribution of microorganisms is essential to understand the ecology of human microbiota. Herein, we assess the possibility of using liposomes loaded with fluorescently labeled nucleic acid mimics (LipoNAMs) to image Gram-negative and Gram-positive bacteria. We proved that liposome fusion...
Chapter
FISH has gained an irreplaceable place in microbiology because of its ability to detect and locate a microorganism, or a group of organisms, within complex samples. However, FISH role has evolved drastically in the last few decades and its value has been boosted by several advances in signal intensity, imaging acquisitions, automation, method robus...
Chapter
Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their targe...
Chapter
Full-text available
Biological materials have inspired the development of new molecules and systems to assist in clinical diagnostics and therapeutics. For clinical and research purposes many different imaging modalities are available to detect or assess the effect of these molecules in humans or animals. The most commonly used are positron or single photon emission c...
Article
Full-text available
Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2′-O-methyl (2′-OMe) RNA modifications have on the probe that is targeting microorganisms is u...
Article
Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determin...
Article
Full-text available
Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H. pylori to antibiotics, antimicrobial nucleic acid mimics are a promising alternative. In particular, locke...
Article
Full-text available
In recent years, there have been several attempts to improve the diagnosis of infection caused by Helicobacter pylori. Fluorescence in situ hybridization (FISH) is a commonly used technique to detect H. pylori infection but it requires biopsies from the stomach. Thus, the development of an in vivo FISH-based method (FIVH) that directly detects and...
Article
Full-text available
The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermodynamics that provide reasonably accurate thermodynamic informat...
Article
Full-text available
While the influence of water in Helicobacter pylori culturability and membrane integrity has been extensively studied, there are little data concerning the effect of this environment on virulence properties. Therefore, we studied the culturability of water-exposed H. pylori and determined whether there was any relation with the bacterium’s ability...
Article
Full-text available
The understanding of the human microbiome and its influence upon human life has long been a subject of study. Hence, methods that allow the direct detection and visualization of microorganisms and microbial consortia (e.g. biofilms) within the human body would be invaluable. In here, we assessed the possibility of developing a variant of fluorescen...
Conference Paper
Helicobacter pylori is a Gram-negative rod that colonizes the human stomach and is associated with the gastrointestinal disorders such as chronic gastritis, peptic ulcer disease, and gastric carcinoma. The over usage of the standard treatments (triple therapy) led to an increased antibiotic resistance. So far, routine antibiotic- resistance charact...
Article
Background. Twenty-five years after the first successful cultivation and isolation of Helicobacter pylori, the scientific community is still struggling to understand the way(s) how this bacterium is transmitted among human populations. Aim. In this work, we aimed at evaluating the likelihood of gastrooral, oral-oral, and fecal-oral routes of infect...
Article
Nicotinamide adenine dinucleotide phosphate oxidase of the vascular cell membrane is an important source of reactive oxygen species. The aim of our study was to evaluate the possible influence of the p22phox C242T gene polymorphism on blood pressure and some markers of oxidative stress in children with tetralogy of Fallot. After surgical repair in...

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