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Nour Eddine Es-Safi

Nour Eddine Es-Safi
Mohammed V University in Rabat. Ecole Normale Supérieure · Chemistry

Professor

About

90
Publications
21,644
Reads
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2,814
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Introduction
Nour Eddine ES-SAFI completed his PhD in organic chemistry in 1997 at Mohammed V University in Rabat, Morocco. He undertook postdoctoral at the French National Institute for Agricultural Research. He is currently working at Mohammed V University in Rabat as deputy director and a full professor. His research focuses on natural products (polyphenols, essential oils, ...), their structural elucidation, their biological activities and their role in food technology. He has published many research papers. He is Editorial Board Member of various scientific journals (Journal of Food Composition & Analysis, Current Bioactive Compounds, ...). He is Associate Editor of Heliyon - Food Science and he is Editor in Chief of the Green and Sustainable Chemistry journal.
Additional affiliations
January 2010 - present
Mohammed V Agdal University, Normal Superior School
Position
  • Professor
September 2003 - August 2005
INRA - National Institute for Agricultural Research
Position
  • Researcher
January 1999 - December 2000
INRA - National Institute for Agricultural Research (INRA)
Position
  • Researcher

Publications

Publications (90)
Article
This study aimed to improve the extractive effect of four solvents and their combinations on phenolic compounds from Moroccan Lavandula stoechas leaves. A simplex-centroid design was used in a solvent system containing ethyl acetate, methanol, ethanol, acetone, and their mixtures to optimize the five responses: extraction yield, total phenol conten...
Presentation
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Molecules Special Issue - Invitation to contribute
Article
Full-text available
Management of broomrape (Orobanche crenata Fosk.) that causes important damages on lentil production becomes a veritable concern in the Mediterranean region. Eighty lentil accessions were evaluated for resistance to O. crenata under field and controlled conditions. Both genotypes ILL6415 and ILL7723 expressed the highest resistance level under fiel...
Article
Full-text available
The current study was performed in order to evaluate the effect of using argane by-products (oil cake and pulp) as fattening diet of lambs. This was achieved through an experimental lamb fattening investigation using argan by-product as feeding source and the exploration of the fattened lamb performance, carcass characteristics and the chemical fat...
Article
Full-text available
Lentil (Lens culinaris Medikus) is one of the most important cool season food legume crops grown in many countries. Seeds are typically rich in protein, fiber, prebiotic carbohydrates and minerals, such as iron and zinc. With changing climate and variability, the lentil crop faces frequent droughts and heat stress of varying intensity in its major...
Article
Rationale: Cotula cinerea of the Asteraceae family is a traditional Moroccan plant, with various biological activities such as analgesic, cytotoxic and antioxidant effects which are often related to the presence of secondary metabolites. The present work aims to screen and identify the main phytochemicals compounds of Cotula cinerea extracts. Met...
Article
Full-text available
The objective of this work was to study the effect of the incorporation of argane by-products (meal and pulp) in ewe’s diet on the production and quality of milk and the performance of lambs in the lactation phase. Twenty ewes were divided into two equal groups. The first one has been fed with argane by-product diet (AD), and the second with a cont...
Article
The acute and subchronic toxicological effects of Salvia verbenaca extracts in hexane, ethyl acetate, n-butanol, were investigated. The extracts were not toxic at 2000 mg Kg⁻¹. A normal body weight evolution was observed during the 14 treatment days where there was no difference between the control and the treated groups. No abnormalities were seen...
Article
Full-text available
The objective of this work was to investigate the cytotoxicological effect of the extracts (hexane, ethyl acetate, and n-butanol) of Cotula cinerea and Salvia verbenaca in addition to the essential oil of Cotula cinerea. These plants are widely used in the Moroccan traditional folk medicine. The cytotoxic effect was explored against two cancer cell...
Article
Background: Salvia verbenaca is a Mediterranean plant currently belonging to the Lamiaceae family. The plant is widely distributed in Morocco and is widely used in Moroccan traditional folk medicine as a cholagogue, antiseptic, diuretic and astringent. The crushed or chopped fresh leaves are also applied in poultices on wounds and emptied abscesses...
Article
Background: Cotula cinerea belongs to the Asteraceae family and grows in desert areas such as Moroccan Sahara. The use of this plant in Moroccan traditional medicine prompted us to investigate its chemical composition, its acute oral toxicity, its analgesic and antioxidative activities. Methods: Extraction was conducted by steam distillation for es...
Chapter
Two new isomeric oxazolidin-2-one derivatives have been synthesized through reaction of (E)-3-styrylquinoxalin-2-one with bis-dichloroethylamine hydrochloride in presence of potassium carbonate. The structures of the formed products were established by NMR and mass spectroscopy. The fragmentation patterns of the two novel synthesized oxazolidin-2-o...
Article
Full-text available
Two new isomeric heterocyclic systems containing oxazolidin-2-one and quinoxaline moieties have been synthesized through reaction of (E)-3-styrylquinoxalin- 2-one with bis-dichloroethylamine hydrochloride in presence of potassium carbonate. The structures of the formed products were established by NMR and mass spectroscopy. The fragmentation patter...
Article
Punica granatum L. is widely recognized for its potency against a broad spectrum of bacterial pathogens. The purpose of this study was to explore the inhibitory and the bactericidal activities of Punica granatum against Salmonella strains. The effect of extracts obtained from different parts (peels, seeds, juice and flowers) of pomegranate and usin...
Conference Paper
Full-text available
Lentil (Lens culinaris subsp. culinaris Medikus) is an important cool-season food legume crop commonly grown under rainfed conditions in South Asia, North America, West Asia and North Africa (WANA). Occurrence of drought and heat stress during the reproductive stage in most of these environments, causing substantial reduction in crop yield. Lentil...
Conference Paper
Full-text available
Lentil (Lens culinaris Medikus) is one of the most important cool season food legume crops. It constitutes good source of seed protein, carbohydrates and minerals particularly to the people living in the developing countries. Terminal drought and heat stress are important factors affecting lentil production especially in the dry and semi-arid regio...
Conference Paper
Full-text available
The root parasite, broomrape (Orobanche crenata) poses a serious threat to lentil production in Mediterranean and West Asian countries. Identification of resistant sources in the germplasm collections and the development of new tolerant varieties could be helpful to address the issue. However, resistance breeding is hampered by the scarcity of gene...
Article
Full-text available
Fenugreek (Trigonella foenum-graecum) is a medicinal plant which is widely used for its pharmacological properties. In this study the phenolic composition of fenugreek crude seeds originating from Morocco has been investigated. Extraction was performed from defatted seeds by a hydromethanolic solution using an Accelerated Solvent Extractor. HPLC te...
Article
Full-text available
Phenolic compounds have a fundamental importance in the nutritional and sensorial characteristics of olive. In this study phenolic compounds extracted from olive fruit stones of three Moroccan registered cultivars were analyzed. The obtained results showed that the total amount of phenolic compounds varied according to the olive variety. The stones...
Article
The phenolic composition of fenugreek (Trigonella foenum-graecum) germinated seeds originating from Morocco has been investigated. After germination, extraction was performed from defatted germinated seeds by a hydromethanolic solution using an Accelerated Solvent Extractor. HPLC coupled to negative ion electrospray ionisation mass spectrometry and...
Article
Flavonoids are a broad class of polyphenolic secondary metabolites widely dispersed throughout the plant kingdom and found in substantial levels in commonly consumed fruits, vegetables and beverages. These compounds have aroused considerable interest because of their potential beneficial biochemical and antioxidant effects on human health. The stru...
Article
Full-text available
Various new heterocyclic compounds were synthesized starting from 4-phenyl-1,5-benzodiazepin-2-one. Catalytic hydrogenation was first explored leading to the formation of compounds where the diazepinic seven bond ring was or was not opened. Further alkylation reactions of the new formed derivatives were achieved in order to prepare new dipolarophil...
Article
The initiatory heterocyclization yields regioselectively the novel pyrazolo[1,5,4-de]quinoxaline (III) whose bromination followed by nucleophilic substitution gives the new tetracyclic system (VII).
Chapter
Full-text available
Mass spectrometry is an important tool for the identification and structural elucidation of natural and synthesized compounds. Its high sensitivity and the possibility of coupling liquid chromatography with mass spectrometry detection make it a technique of choice for the investigation of complex mixtures like raw natural extracts. The mass spectro...
Article
New pyrazolo[1.5.4-de]quinoxaline derivatives were prepared by the action of 7-aminoindazole 1 on diethyl and dimethyl acetylene dicarboxylates. The structures of the obtained compounds and the direction of cyclization were investigated through a crystallographic study of compound 2. Further alkylation, hydrogenation, and bromination were also expl...
Article
Full-text available
New 3-hydroxy-1,5-benzodiazepin-2-ones were synthesized through condensation between o-phenylenediamines with glycidic ester. Alkylation and oxidation of some of the obtained compounds were also explored in different conditions yielding various oxidized and alkylated benzodiazepines. The structural elucidation of the synthesized compounds was achie...
Article
Full-text available
The fragmentation patterns of a series of three novel synthesized 3-hydroxy-4-phenyl-tetrahydro-1,5-benzodiazepin-2-ones (1-3), possessing the same backbone structure, were investigated using electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) techniques. A simple methodology, based on the use of ESI (positive io...
Article
Two new colorless phenolic compounds were formed from malvidin 3- O-glucoside incubated in an ethanolic solution. Their structures were characterized by means of one- and two-dimensional NMR analysis and through electrospray ionization-mass spectrometry. As compared to the structure of the initial anthocyanin skeleton, the first new compound showed...
Article
NMR and MS techniques were used for the unambiguous structural elucidation of synthesized modified monomeric and dimeric flavan-3-ols presenting different substituents on the A-ring C8 position. The full characterization of the synthesized compounds was achieved by concerted use of NMR and ESI-MS techniques. Assignments of proton and carbon atoms w...
Article
Several flavonols have been transformed upon treatment by Trametes versicolor laccase. Most of the major oxidation products have been isolated by HPLC as pure compounds and their structures have been, when possible, investigated through spectral methods (HPLC–MS and NMR). The results are coherent with the predominance of a dismutation process, lead...
Article
The antioxidant activity of the Globularia alypum phytochemicals were evaluated for their capacity to scavenge the 1,1-diphenyl-2-dipicrylhydrazyl (DPPH°) free radical and some structure–activity relationships were obtained. Assay guided fractionation led to the isolation of syringin, four phenylethanoids, four flavonoids and six iridoids as the ma...
Article
The structure of globularin was studied by a mass spectrometric methodology based on the combined use of positive and negative electrospray ionization, collision‐induced dissociation (CID), and tandem mass spectrometry. The mass spectrometry investigation was achieved through in‐source fragmentation of the deprotonated [M−H], protonated [M+H], lith...
Article
A quinoxaline‐2,3‐dione derivative was synthesized, and its chemical structure was determined through spectral analysis. Alkylation of this compound under phase transfer catalysis (PTC) conditions yielded monoalkylated and diakylated adducts. The monolalkylation process was shown to be regioselective occurring on the quinoxalic nitrogen atom rather...
Article
Full-text available
In a screening program for free radical scavengers, new antioxidant compounds have been obtained by C-8 substitution of various groups on (+)-catechin A ring. Their preparation, purification, identification by MS, 1H and 13C NMR analysis as well as their antiradical/antioxidant properties are reported. In order to explore the substituent effect on...
Article
Several flavonols were chemically transformed upon treatment by Trametes versicolor laccase; the majority of the major oxidation products were isolated (HPLC) as pure compounds and their structures unambiguously established through spectroscopic methods (HPLC-MS), when possible. In some cases, compounds were difficult to obtain either completely pu...
Article
Mass spectrometric methodology based on the combined use of positive and negative electrospray ionization, collision-induced dissociation (CID) and tandem mass spectrometry (MS/MS) has been applied to the mass spectral study of a series of six naturally occurring iridoids through in-source fragmentation of the protonated [M+H]+, deprotonated [M--H]...
Article
Full-text available
Phenolic compounds form one of the main classes of secondary metabolites. They display a large range of structures and they are responsible for the major organoleptic characteristics of plant-derived-foods and beverages, particularly color and taste properties and they also contribute to the nutritional qualities of fruits and vegetables. Phenolic...
Article
Full-text available
Objective: To investigate the in vitro antioxidant activity of the hydromethanolic extract of the aerial parts (leaves and stems) of Salvia verbenaca L. towards fatty acids (linoleic and linolenic acids) and human, low density lipoproteins (LDL) peroxidation. Materials and Methods: Lipid peroxidation was carried out in the presence of the S. verben...
Article
The structure of a polymeric proanthocyanidin fraction isolated from pear juice was characterized by NMR, ESI/MS, and MALDI-TOF/MS analyses, and its antioxidant activity was investigated using the DPPH free radical scavenging method. The results obtained from 13C NMR analysis showed the predominance of signals representative of procyanidins. Typica...
Article
The effect of an electron attracting substituent in the Lewis acid catalyzed oligomerization of flavanols was investigated. The results showed that the presence of a COCF3, at the 8 position of a (+)-catechin unit strongly influenced the attack of the 6 free nucleophile flavanol position by the electrophile generated from a 4-O-alkyloxy protected c...
Article
Benzylic oxidation of catechine has been performed using either DDQ or Ce(IV) trifluoromethanesulfonate as oxidizing agent. These procedures allowed the efficient synthesis of taxifolin 3 in a high overall yield.
Article
From the hydromethanolic extract of the aerial parts of Globularia alypum grown in Morocco, a new chlorinated iridoid glucoside, globularioside has been isolated beside 5 known iridoid glycosides, globularin, globularicisin, globularidin, globularinin and globularimin. This is the first report of a chlorinated iridoid in G. alypum and in the Globul...
Article
A mass spectrometric method based on the combined use of positive and negative electrospray ionization, collision-induced dissociation and tandem mass spectrometry has been applied to the structural characterization of the eriodictyol 7-O-glucosyl-(1→2)-glucoside and luteolin 7-O-glucosyl-(1→2)-glucoside. The low-energy product ion mass spectrum of...
Article
Mass spectrometric methodology based on the combined use of positive and negative electrospray ionization, collision-induced dissociation (CID) and tandem mass spectrometry (MS/MS) has been applied to the structural characterization of 6-hydroxyluteolin 7-O-glucoside and 7-O-glucosyl-(1 --> 3)-glucoside. In-source fragmentation of both glycosides a...
Article
Full-text available
Objective : To investigate the in vitro antioxidant activity of the hydromethanolic extract of aerial parts (leaves and stems) of Globularia alypum L. toward linoleic acid emulsion and human low-density lipoproteins (LDL) peroxidation. Materials and Methods : Lipid peroxidation was carried out in the presence of G. alypum hydromethanolic extract (1...
Article
Three new phenolic compounds were isolated from the aerial parts of Globularia alypum. Their structures were determined as 6-hydroxyluteolin 7-O-laminaribioside (1), eriodictyol 7-O-sophoroside (2), and 6'-O-coumaroyl-1'-O-[2-(3,4-dihydroxyphenyl)ethyl]-beta-D-glucopyranoside (3). In addition, three phenylethanoid glycosides (acteoside, isoacteosid...
Article
The regioselective introduction of substituents at C-8 of (+)-catechin is described, leading to the synthesis of several catechin derivatives with various substitution patterns to be used for the further synthesis of modified proanthocyanidins. Thereafter, a new 3-O-4 ether-linked procyanidin-like derivative was synthesized. Its formation was selec...
Article
A general procedure for the oxidation of catechin derivatives is described, leading to the introduction of a new hydroxy group at C-6. This procedure has been applied for the synthesis of elephantorrhizol, a natural flavan-3-ol exhibiting a fully substituted cycle A.
Article
A xanthylium salt pigment was synthesized from the reaction involving glyoxylic acid and (+)-catechin. After isolation, its spectral characteristics and colour intensity, influenced by pH, were studied. When varying pH from acidic to alkaline values, a steady bathochromic displacement of the visible wavelength was observed while no noticeable chang...
Chapter
During storage and ageing of red wine, the pigment composition becomes progressively more complex. There is a steady shift from the original red color due to the anthocyanins extracted from grapes to a more yellow tint. This is explained by the progressive and irreversible conversion of anthocyanins and of flavanols to more stable pigments, includi...
Chapter
During storage and ageing of red wine, the pigment composition becomes progressively more complex. There is a steady shift from the original red color due to the anthocyanins extracted from grapes to a more yellow tint. This is explained by the progressive and irreversible conversion of anthocyanins and of flavanols to more stable pigments, includi...
Article
The reaction between (+)-catechin and glyoxylic acid was investigated as a model system of color changes usually observed during conservation and ageing of fruit-derived foods. The reaction was monitored by LC/DAD and LC/ESI-MS analysis and the formation of various phenolic compounds initially absent in the mixture was observed.Among the detected c...
Article
Summary Autoxidation of (+)-catechin in a model solution system, containing cupric ions, was monitored by high-performance liquid chromatography with diode array detection. The rate of (+)-catechin oxidation and the extent of browning increased with the copper concentration, showing an effect of copper on browning of flavanols. The progression of t...
Article
The reactions between (epi)catechin, mavidin 3-O-glucoside, and some aldehydes were investigated by LC/DAD and LC/ESI-MS analysis. The obtained results showed that the acetaldehyde-mediated condensation occurred more generally and glyoxylic acid, furfural, and 5-(hydroxymethyl)furfural (HMF) react in the same way in the first stages of the reaction...
Article
The reaction between (+)-catechin and cyanidin 3-O-glucoside was investigated in the presence of furfural and 5-(hydroxymethyl)furfural using LC/DAD and LC/MS analysis, and the obtained results were compared with those recorded with malvidin 3-O-glucoside. The appearance of colorless and red and yellow compounds was observed showing that the two po...
Article
Various phenolic compounds were synthesized in an aqueous–alcoholic solution containing (+)-catechin and glyoxylic acid which was used as a model of fruit-derived food browning that usually occurs during aging. After purification by semi-preparative HPLC, the isolated compounds were subjected to homo- and heteronuclear proton and carbon NMR analysi...
Article
Summary The reaction of (+)-catechin in wine-like model solution was investigated. First appearance of colourless dimeric compounds consisting of two flavanol units linked by carboxy-methine bridge was observed. Their isolation and further incubation was found to yield two types of yellowish pigments showing visible absorption maxima at 440 and 460...