Norma M de la Fuente-Salcido

Norma M de la Fuente-Salcido
Autonomous University of Coahuila | UAdeC · Graduate Program in Biochemical Engineering Dept Bioprospecting and Bioprocesses Biological Sciences Faculty

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93
Publications
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550
Citations
Citations since 2017
48 Research Items
368 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
Additional affiliations
May 1991 - October 2020
Autonomous University of Coahuila
Position
  • Professor
Description
  • Research in proteins, antimicrobial peptides, chitinases, edible coatings and nanocaotins, bioactive metabolites (microbial and vegetables) Postgraduate Professor in Biochemical Engineering

Publications

Publications (93)
Article
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RESUMEN La mastitis se considera la enfermedad más costosa que afecta a la industria lechera. Muchas estrategias implican el uso de antibióticos para prevenir y tratar esta enfermedad, mismos que generan cepas multi-resistentes a antibióticos. La exigencia de reducir los trazas de antibióticos en los alimenticios de origen animal ha llevado a los i...
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Resistance or multi-resistance to antibiotics by microorganisms has generated one of the most emerging problems of public health due to the loss of susceptibility to drugs. The World Health Organization (WHO) publishes the groups of bacteria considered antibiotic-resistant "priority pathogens", and for these microbes new antimicrobials or strategic...
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Worldwide it has been confirmed that most of the chemical/artificial additives used to preserve food are causing undesirable damage to human health, and currently the consumers are demanding the change of potentially harmful ingredients by safe and natural compounds. According to this demand, there is growing interest in expanding the use of natura...
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RESUMEN: Ariocarpus fissuratus es una cactácea endémica de Coahuila ubicada principalmente en la Reserva Municipal Sierra y Cañón de Jimulco (RMSCJ) la cual se encuentra en el apéndice I del CITES así como en la NOM-059-SEMARNAT-2010 como especie en peligro de extinción. La apreciación de esta planta tanto por su belleza exótica así como por su sup...
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Resumen-El objetivo de esta investigación fue monitorear el crecimiento de Saccharomyces cerevisiae en mosto a base de harina de nopal y poder emplear el nopal como una fuente de biomasa para la generación de etanol. Se empelaron dos variedades de nopal (Atlixco y Blanco) como fuente de biomasa lignocelulolítica, inicialmente la harina de nopal fue...
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Palabras clave: Inhibición, Xoconostle, semilla. Introducción: El xoconostle es el fruto de la Opuntia joconostle, una planta de la familia de las cactáceas (Cactaceae) con cerca de 124 géneros con 1438 especies que agrupan 60 géneros y 670 especies en México (77% endémicas) [1]. Las semillas son el principal órgano reproductivo de la gran mayoría...
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New and improved approaches are urgently needed to fight the increasing number of multi-drug resistant bacteria. The antibacterial effect of silver nanoparticles (AgNPs) prepared by standardized chemical and biological syntheses is compered here. Biological systems included extracts of Opuntia ficus-indica mucilage and extracellular growth broth of...
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Nowadays, consumers are demanding nutrient‐rich products for health optimal benefits. In this regard, Brassicaceae family plants, previously named cruciferous, group a large number of widely consumed species around the world. The popularity of Brassica is increasing due to their nutritional value and pharmacological effects. The group includes a la...
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Fungi are the primary infectious agents in plant crops and many post-harvest fungal diseases of fruit and vegetables causing significant economic losses worldwide. Here, the antifungal effect of Prosopis glandulosa extract (PgE) against phytopathogenic fungi was evaluated. The effect with PgE (5, 4, 2, 1, 0%) as AI (%) and radial growth rate reduct...
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The microbiology of the rhizosphere has been investigated since 1904, revealing innumerable rhizomic microorganisms that promote growth in swamps (RPCPs), which have attracted a special interest to improve agroecological practices, crop productivity and protect soils from environmental contamination under a agricultural sustainability approach. Thi...
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Currently, cancer is one of the leading causes of death worldwide. The latest reports from the World Health Organization (WHO) of 2018 show alarming figures that indicate 18.1 million new cases and recorded 9.6 million deaths from cancer, with a 5-year prevalence of 43.8 million patients. The low efficiency of conventional cancer treatments has gen...
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Existen plantas que contienen un gran número de metabolitos secundarios, que son compuestos que se generan sobre todo en plantas que viven en condiciones ambientales adversas como lo son las regiones áridas. Estos metabolitos secundarios son generados además como mecanismo de defensa contra insectos y patógenos, por lo cual se les atribuye que son...
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RESUMEN: Los nanopelículas comestibles sintetizados con polímeros y agentes bioactivos vegetales han confirmado su eficacia para preservar frutas y verduras de diversos orígenes. Se sintetizaron cuatro nanopelículas comestibles diferentes con extracto de chitosan-mezquite (Prosopis glandulosa) y se caracterizaron parcialmente con respecto al color...
Article
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RESUMEN: Los polímeros antimicrobianos como el quitosano y los extractos vegetales (Prosopis glandulosa) son eficientes agentes bioactivos para inhibir el crecimiento de bacterias, hongos y levaduras y han generado novedosas investigaciones para utilizarlos como una alternativa real y efectiva para inhibir patógenos, sin generar resistencia como lo...
Chapter
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Polymer-based edible coatings were designed to extend the shelf-life of foods by controlling their intrinsic and extrinsic parameters. In addition, a coating reduces microbiological damage that affects the quality, which in turn causes high economic losses during post-harvest storage. Although polymer coatings with natural bioactive agents have con...
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Biocontrol de fitopatógenos e incremento del vigor en semillas de tomate (Solanum lycopersicum L.) por Bacillus thuringiensis y sus bacteriocinas Biocontrol of phytopathogens and increased vigor in tomato seeds (Solanum lycopersicum L.) by Bacillus thuringiensis and its bacteriocins ID 1 er Autor: Norma Margarita, De La Fuente-Salcido / ORC ID: 000...
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Bacillus thuringiensis is a microbial insecticide whose presence in the Fernandez Canyon State Park, a protected natural area in the north of Mexico, has not been reported. The objective of this work was to isolate Bacillus thuringiensis strains isolated from the Fernandez Canyon State Park with the capacity to synthesize antimicrobial peptides (ba...
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Biocontrol de fitopatógenos e incremento del vigor en semillas de tomate (Solanum lycopersicum L.) por Bacillus thuringiensis y sus bacteriocinas Biocontrol of phytopathogens and increased vigor in tomato seeds (Solanum lycopersicum L.) by Bacillus thuringiensis and its bacteriocins ID 1 er Autor: Norma Margarita, De La Fuente-Salcido / ORC ID: 000...
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Palabras clave:Quitinasas, bacteriocinas, lipasas Introducción En la actualidad diversos metabolitos biosintetizados por el género Bacillus, se encuentran en desarrollo para ser considerados como una importante alternativa para generar diferentes productos biológicos efectivos para inhibir el desarrollo de bacterias dañinas y hongos fitopatógenos....
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Resumen En años recientes ha surgido la necesidad de desarrollar nuevas metodologías de conservación de productos hortofrutícola; la fresa es un fruto con un alto contenido en antioxidantes y muy apreciada por sus características sensoriales. En el presente estudio se analizó el comportamiento de las características físico-químicas y sensoriales de...
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Palabras clave: péptidos antimicrobianos, biopelículas, actividad antimicrobiana. Introducción Una biopelícula o recubrimiento se define como un empaque primario formado por componentes comestibles, que mejoran las propiedades los alimentos y alargan su vida de anaquel [1]. Los principales componentes de la matriz estructural de los recubrimientos...
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Resumen Las películas comestibles utilizadas en los alimentos deben de proteger y extender su vida útil, es por ello que se busca que tengan características de barrera contra agentes externos; por lo que el objetivo del presente trabajo fue evaluar las propiedades mecánicas y la permeabilidad al vapor de agua de películas elaboradas a base de quito...
Data
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Figure S1. Inhibition of rChiA Btt on the growth of C. gloeosporioides using the well diffussion assay. (A) The fungus was placed into the center of fresh potato dextrose agar and different chitinase concentrations were added in each well. Wells (a) without chitinase or added with (b) 18.75 U, (c) 9.38 U, (d) 4.69 U, (e) 3.34 U, and (f) 1.17 U. (B)...
Article
Mastitis caused by microbial infections in dairy goats reduces milk yield, modifies milk composition, and potentially contributes to morbidity in herds and consumers of dairy products. Microorganisms associated with mastitis in dairy goats are commonly controlled with antibiotics, but it is known that continued use of these chemical agents promotes...
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Bacillus thuringiensis subsp. tenebrionis DSM-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. The endochitinase chiA Btt gene of B. thuringiensis subsp. tenebrionis DSM-2803 was cloned and expressed in Escherichia coli. The recombinant 6x-histidine tagg...
Article
Currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. In particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central Mexico. Our objective was to isolate bacteria, from goat products, that synthesized ant...
Article
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RESUMEN El objetivo principal de ésta investigación fue evaluar la capacidad de Ambrosia ambrosioides para absorber y acumular plomo (Pb) en sus tejidos e identificar cual parte de la planta acumulaba el metal en mayor cantidad. Para esto, se hizo crecer a las plantas en un suelo artificial (perlita mineral) y se expusieron a 3 diferentes concentra...
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ABSTRACT The search for new compounds is the main purpose of bioprospecting and is an inexhaustible source of new genes, antimicrobials, enzymes, proteins and other bioactive compounds. The initial stage of this project bioprospecting in soil samples from the floodplain Cañón de Fernández State Park in Lerdo Durango, Mexico provided the date, 108 i...
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ABSTRACT Due to the serious problem of obesity in Mexico, the trend is to develop low calorie products with high nutritional value and easy to consume. The objective of this study was to develop a low calorie jelly, also rich in proteins, using protein from soybeans and strawberries as a source of antioxidants. Sensorial evaluation was performed, M...
Article
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ABSTRACT In this Project, the study was performed with two isolates from fermented beverages, tepache and pulque, the concentrations of the main carbon source is varied, in this case tree levels, high, lower and normal commonly used for fructose, sucrose and glucose were taken. The essays was incubated and purified by centrifugation and pH adjustme...
Article
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ABSTRACT: The efficiency in vitro and in vivo of a bioactive coating formulated with chitosan and mesquite extract was investigated for postharvest biocontrol of plant pathogenic fungi (C. gloeosporoides, F. oxysporum, R. oryzae and R. stolonifer) in fruit and vegetables (strawberry, cherry tomatoes and baby carrots). Separately chitosan and mesqui...
Article
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quitosana es un derivado desacetilado de la quitina con capacidad antifúngica y antibacteriana. Los objetivos de la investigación fueron el aislar e identificar molecularmente hongos fitopatógenos y evaluar el efecto antifúngico de la quitosana de medio y bajo peso molecular. Los hongos aislados de frutas y hortalizas se identificaron por caracterí...
Article
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ABSTRACT Nisin is an important lantibiotic for the food industry, for that reason is essential to have a method for nisin quantification. In order to have a measurement way it was studied the nisin effect on the bioluminescence of L. monocytogenes EGD-e::pPL2lux-PhlyA, a recombinant strain with constitutive luminescence. Experiments shown a signifi...
Article
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ABSTRACT: Goat’s milk is estimated to be one of the most consumed milk in the world, mainly in a liquid form, being it an important source of probiotic organisms and probably of microorganisms that synthetise antimicrobial peptides. However, to date there is little research on the matter, and in this work we focus our effort in the isolation and id...
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ABSTRACT: The purpose of this study was to assess the in vitro and in vivo antifungic activity of extract from mesquite (EM) Prosopis glandulosa to evaluate the fungitoxic potential to enrich the formulation of coatings and/or films to prolong the quality and shelf life of fruits and vegetables and protecting them from biological damage. The EM was...
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ocuidad para el medio ambiente y además de que no representan un riesgo para la salud de los consumidores. Palabras clave: Fresa, fitopatógeno, biocontrol. Abstract Strawberry is a very important agricultural product in the generation of foreign exchange in Mexico, considered the fourth largest producer worldwide country. However, strawberry cro...
Chapter
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Bacillus thuiringiensis es la bacteria entomopatógena más reconocida en el mundo y un sistema de estudio fascinante para los investigadores interesados en ampliar el conocimiento sobre la diferencia estructural y la actividad biológica de los metabolitos que sintetiza. Las bacteriocinas sintetizadas por este bacilo, son metabolitos de secreción de...
Article
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Mexican Tuba (M-Tuba) and Tepache are Mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. At present, reports on the microbiota and nutritional effects of both beverages are lacking. The purpose of this study was to determine whether M-Tuba and Tepache contain cultivable lactic acid bacteria (LAB) capable...
Article
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Antibiotic resistance by bacteria is one of the major emerging issues in human and animal health, due to the loss of susceptibility to certain drug's action. This review discuss the terminology and international reference guidelines that allow a right study of the antimicrobial agents' effect and/or their combinations in vitro and in vivo. They're...
Chapter
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PROLOGO Desde sus orígenes casi primitivos la microbiología ha sido el centro de atención de incontables investigadores que comparten la fascinación por el conocimiento del mundo microbiano, y a través de sus descubrimientos, han coadyuvado en la transformación para posicionarla en una área de investigación de frontera especializada, sofisticada y...
Article
Full-text available
Antibiotic resistance by bacteria is one of the major emerging issues in human and animal health, due to the loss of susceptibility to certain drug’s action. This review discuss the terminology and international reference guidelines that allow a right study of the antimicrobial agents’ effect and/or their combinations in vitro and in vivo. They’re...
Article
Full-text available
Bacteriocins synthesized by entomopathogenic Bacillus thuringiensis are gaining attention owing to their inhibitory effects against a wide variety of pathogenic bacteria. In the present study, we purified and characterized Tolworthcin 524, a bacteriocin synthesized by B. thuringiensis subsp. tolworthi, and compared it with other bacteriocins synthe...
Article
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A ctualmente la fresa está conside-rada en nuestro país como uno de los productos agrícolas más importan-tes en cuanto al volumen de siembra y el valor de la producción (SAGARPA, 2013), por lo tanto su producción im-pacta directamente en la desarrollo de empleos y en la generación de impor-tantes divisas en nuestro país. La fresa (Fragaria spp. Fam...
Article
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Various strains of Bacillus thuringiensis are among the most successful entomopathogenic bacteria used commercially as biopesticides owing to their ability to synthesize insecticidal crystal (Cry) and cytolytic (Cyt) protein toxins during sporulation, and vegetative insecticidal (VIPs) proteins during the vegetative phase of growth. Whereas much is...
Article
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In the present study, we expressed the chiA74 gene of Bacillus thuringiensis in Escherichia coli K12 and demonstrated that the active ChiA74 enzyme was produced at a high level in this strain. The ChiA74 enzymatic activity (in units per milliliter) was approximately 500 % greater in E. coli K12 when compared to that produced in E. coli DH5α. Moreov...