Norihide Nakada

Kanagawa University · Faculty of Engineering

Pharmaceutical and personal care products (PPCPs) recently defined as emerging pollutants that widespread in surface water all around the world. This study investigated the distribution, and ecological risk of PPCPs in urban rivers of Hanoi, Vietnam, and Metro Manila, the Philippines. Of the 56 investigated PPCPs, 48 and 33 compounds were detected...

Background Excrement containing antimicrobial-resistant bacteria (ARB) is discharged from the hospital sewage through wastewater treatment plants (WWTP) into rivers, increasing the antimicrobial resistance (AMR) burden on the environment. Purpose We illustrate the contamination of hospital sewage tanks with ARB harboring antimicrobial resistance g...

N-nitrosodimethylamine (NDMA) and N-nitrosomorpholine (NMOR) and their specific precursors (N,N-dimethylformamide [DMF] for NDMA and morpholine [MOR] for NMOR) were widely identified in runoff of urban area around the southern Lake Biwa basin, Japan. It was thought that this runoff might constitute a non-point source of the four compounds in rivers...

This study evaluated the occurrence of PPCPs in Cau River (Vietnam). Surface water and sediment samples were collected to determine PPCP concentrations. The analysis results showed the presence of 36 out of 56 investigated PPCPs in samples. The total concentration of PPCPs in water samples ranged from 8.21 to 529 ng/L and the value observed in sedi...

Disinfection by-products (DBPs) discharged from sewage treatment plants (STPs) could harm downstream receiving waters and drinking water resources. In-stream attenuation of photo- and non-photodegradable DBPs during river transportation is currently not well understood. Here we sought to fill this knowledge gap by meta-data-analysis for modeling in...

The contribution of specific precursors to N-nitrosodimethylamine formation potential (NDMA FP) upon chloramination depends not only on their NDMA molar yields but also on their concentrations in the actual environment. We investigated the seasonal and diurnal patterns of the NDMA precursor N,N-dimethylformamide (DMF) and NDMA FP in the Yodo River...

N-nitrosodimethylamine (NDMA), a probable carcinogenic disinfection by-product, can be formed with high molar yields following chloramination of ranitidine (RNTD), a histamine H2-receptor antagonist. Although RNTD and some of its transformation products (TPs) have been studied under chlorination and photo-irradiation, the relationship between RNTD...

Background The dissemination of antimicrobial-resistant bacteria (ARB) and the transfer of antimicrobial resistance genes (ARGs) are a threat to public health. Antibiotics are indispensable therapeutic agents essential for the treatment of infectious diseases; however, inappropriate use of antibiotics leads to the emergence of ARB. It is establishe...

Knowledge of the hazards and associated risks from chemicals discharged to the environment has grown considerably over the past 40 years. This improving awareness stems from advances in our ability to measure chemicals at low environmental concentrations, recognition of a range of effects on organisms, and a worldwide growth in expertise. Environme...

Pharmaceuticals raise concerns for aquatic species owing to their biological activities. It is estimated that nearly 40% of marketed pharmaceuticals target G protein-coupled receptors (GPCRs). Using an in vitro transforming growth factor-α (TGFα) shedding assay, we previously detected antagonistic activities of GPCR-acting pharmaceuticals against a...

To determine the most efficient pretreatment for ceramic membrane filtration (CMF) of primary clarifier effluent (PE), the effectiveness of ozonation and coagulation was investigated from the viewpoint of both virus removal and mitigation of membrane fouling. Our results showed virus removal by coagulation to be more efficient as a CMF pretreatment...

N-nitrosodimethylamine (NDMA) and formaldehyde (FAH) are probable carcinogenic disinfection by-products and have been found to occur in areas of the Yodo River basin that are influenced by treated wastewater. The diurnal patterns of them were examined with water samples collected over 4 years in different seasons at five outlets of four sewage trea...

The seasonal and diurnal patterns of N-nitrosomorpholine (NMOR) and its formation potential (NMOR FP) were examined with water samples taken from five outlets of four sewage treatment plants (STPs), seven main stream sites, and five tributary sites in the Yodo River basin. STPs were shown to be the main sources of downstream NMOR load. The highest...

We investigated the effects of the inclusion of biological activated carbon (BAC) on membrane fouling in combined process of ozonation, coagulation and ceramic membrane filtration (O3 + PACl + CMF) for treating secondary effluent. Inclusion of BAC between ozonation and coagulation reduced membrane permeability. The normalized flux decreased to 90%...

Whilst pharmaceuticals are now routinely detected in aquatic environments, we know little of the biological activity their presence might provoke. It is estimated that nearly 40% of all marketed pharmaceuticals are G protein-coupled receptors (GPCRs)-acting pharmaceuticals. Here, we applied an in vitro assay, called the TGFα shedding assay, to meas...

This study investigated the removal characteristics of N-Nitrosamines and their precursors at three pilot-scale water reclamation plants. These plants applies different integrated membrane systems: (1) microfiltration (MF)/nanofiltration (NF)/reverse osmosis (RO) membrane; (2) sand filtration/three-stage RO; and (3) ultrafiltration (UF)/NF and UF/R...

This study investigated the removal characteristics of N-nitrosamines and their precursors at three pilot-scale water reclamation plants. These plants applies different integrated membrane systems: (1) microfiltration (MF) / nanofiltration (NF) / RO; (2) sand filtration/three-step RO; and (3) ultrafiltration (UF) / NF and UF / RO. Variable removal...

Emerging contaminants in the aquatic environment have become a worldwide problem. Conventional wastewater treatment processes are ineffective for eliminating the emerging contaminants at trace concentrations. Nanomaterials possessing novel size-dependent properties, however, have shown great potential for removing these contaminants. Herein we revi...

Fifty-five pharmaceuticals were monitored at four rivers and inlets and/or outlets of three sewage treatment plants (STPs) in Yodo River watershed, Japan over 17 sampling events. Twenty-six quantified pharmaceuticals were classified by source and fate. The load per person (LPP) of nine pharmaceuticals, including six with observed mass balance in st...

Little is known about the mechanisms influencing the differences in attenuation of antibiotics between rivers. In this study, the natural attenuation of four antibiotics (azithromycin, clarithromycin, sulfapyridine, and sulfamethoxazole) during transport along the Thames River, UK, over a distance of 8.3 km, and the Katsura River, Japan, over a dis...

Water samples were collected from effluent-dominated urban streams in Tokushima, Kyoto, and Saitama in Japan to roughly determine the contribution of pharmaceuticals and personal care products (PPCPs) and surfactants to whole toxicity of the water. Approximately 100 PPCPs including anionic surfactants such as linear alkylbenzene sulfonate (LAS), we...

We investigated virus removal and the formation of disinfection by-products by a combined ozonation, coagulation, and ceramic membrane filtration process (O3 + PACl + CMF) for water reclamation. Pilot-scale continuous operation used secondary effluent as test water, and virus removal performance was tested using F-specific bacteriophage MS2. The ra...

Rapid increases in the amounts of fullerene C60 nanoparticles (nC60) being produced and used will inevitably lead to increases in the amounts released into the aquatic environment. This will have implications for human and ecosystem health. Wastewater treatment plants are key barriers to nC60 being released into aquatic systems, but little informat...

Photoproducts of pharmaceuticals have been studied in order not to overlook their potential risks to aquatic organisms. However, no studies have verified an equation for predicting the fate of photoproducts in aquatic environment (Poiger equation) by field measurements, leaving uncertainties in its practical utility. Therefore, we conducted this st...

The arrival and discharge of seven antibiotics were monitored at two trickling filter sewage treatment plants of 6000 and 11,000 population equivalents (PE) and two activated sludge plants of 33,000 and 162,000 PE in Southern England. The investigation consisted of 24 h composite samples taken on two separate days every summer from 2012 to 2015 and...

The quality of surface waters in lowland rivers is largely dependent on the efficiency of wastewater treatment. Even in the developed countries, there have been difficulties in evaluating the effectiveness of wastewater management and the proportion of wastewater content (WWC) in the river, as well as in estimating the contributing human population...

Huge amount of the chemicals are used in industry. Although the chemicals are statutory restricted, formation and/or transformation of them to hazardous chemicals in urban water cycling have not been taken into consideration. This research was aimed at establishing the formation potential screening tests of eight N-nitrosamines, four aldehydes and...

Main objective of this study was to study on the behavior of perfluorinated carboxylates (PFCAs) and their formation potentilas (9 PFCA-FPs) during river transport where the main water source is treated wastewater. 9 PFCAs, 14 precursors and 9 PFCA-FPs were analyzed from wastewater (n=2), river water (n=35), and river sediment (n=7). A s a result,...

This study describes the occurrence, fate,and removal of free estrogens (estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethynylestradiol (EE2)) and their glucuronide and sulfate conjugates (estrone-3-sulphate (E1-3S), 17β-estradiol-3-sulfate (E2-3S), estriol-3-sulfate (E3-3S), estrone-3-glucuronide (E1-3G), 17β-estradiol-3-glucuronide (E2-3G),...

A year-round monitoring survey of sewage flowing into sewage treatment plants located in urban Japan was conducted by targeting seven representative pharmaceutical components-atenolol (ATL), ciprofloxacin (CFX), clarithromycin (CTM), diclofenac (DCF), diltiazem (DTZ), disopyramide (DSP), and sulpiride (SPR)-detected in the river environment. For ea...

To reduce the risk of producing an anti-influenza drug-resistant virus from wildfowl, it is important to estimate the concentrations of anti-influenza drugs in river water during an influenza pandemic and to evaluate the concentrations that keep river basins safe. We first created a newly designed infectious disease transmission model based on the...

We analyzed pepper mild mottle virus (PMMoV) in 36 samples taken from surface water, wastewater, groundwater, tap water and bottled water in Hanoi, Vietnam. We then compared the occurrence and fates of PMMoV with pharmaceuticals and personal care products (PPCPs), which are known wastewater tracers. PMMoV was detected in 94% of the surface water sa...

To improve the risk assessment of pharmaceuticals, it is helpful to know how rapidly they are removed from river water. Direct photolysis by sunlight could be an important process, but so far few studies have attempted to compare modeled with actual losses in a river. Therefore, we quantified natural attenuation by monitoring 56 pharmaceuticals and...

We optimised the simultaneous analysis of four analytes, namely three anti-influenza drugs (oseltamivir (OS), zanamivir (ZAN) and amantadine (AMN)) and one metabolite of OS (oseltamivir carboxylate (OC)), in sewage treatment plant (STP) influent and effluent by verifying the types and conditions of solid phase extraction (SPE) appropriate for LC-MS...

Endocrine-disrupting chemicals are exogenous substances that alter the function of the endocrine system, with adverse health effects on organisms or their progeny. In vitro estrogen receptor (ER) reporter gene assays have long been used to measure estrogenic activity in wastewater. Nevertheless, there is still uncertainty about their usefulness in...

We established a method for the simultaneous analysis of seven preservatives and five antimicrobials in water. These chemicals are widely used in cosmetics, and their presence in river water is of concern. We used the method to test 13 sites in Japanese rivers and streams contaminated by domestic wastewater, effluent, or industrial wastewater. 2-Ph...

Main objective of this study was to examine the behavior of perfluorocarboxylic acids (PFCAs) during treatment processes at a WWTP by (a) analysis of PFCAs at each treatment process (b) study on the examination methods of formation potential of PFCAs from their precursors in wastewater samples (c) calculation of mass flow of PFCs through treatment...

Existing stochastic models for predicting concentrations of down-the-drain chemicals in aquatic environments do not account for the diurnal variation of direct photolysis by sunlight, despite its being an important factor in natural attenuation. To overcome this limitation, we developed a stochastic model incorporating temporal variations in direct...

N-nitrosamines are well known as carcinogens present in the environment. However, studies of the occurrence and fate of N-nitrosamines and their N-nitrosamine formation potential (FP) in wastewater treatment plants (WWTPs) are lacking. Therefore, the objectives of this study were to determine the occurrence of N-nitrosamines in WWTPs, the FP of N-n...

In February 2011, at the peak of an influenza outbreak, we performed a comprehensive analysis of the mass balances of four anti-influenza drugs-oseltamivir (OS), oseltamivir carboxylate (OC), amantadine (AMN), and zanamivir (ZAN)-in the urban area of the Yodo River system. This area includes three main river catchments (the Katsura, Uji, and Kidzu...

The increasing production and use of fullerene C60 nanomaterials raises concerns about environmental risks and human health. However, the materials’ behavior and fate during wastewater treatment are still unclear. We investigated the adsorption of aqueous nanoscale fullerene (nC60) on activated sludge by analysis of adsorption kinetics and equilibr...

Despite the fact that some hydrophobic pharmaceuticals and personal care products (PPCPs) have been found to accumulate in river sediments, little is known about the contribution of these compounds to the toxicity of the whole sediment. We sampled river sediments from two urban creeks with an unsewered drainage area to investigate the toxicity for...

The fate and removal of 48 pharmaceuticals and personal care products (PPCPs) during anaerobic digestion of sewage sludge were investigated in four full-scale sewage treatment plants (STPs). We measured concentrations in both the liquid and solid phases of the sludge to compare the distribution ratios (Kd) between phases before and after digestion....

A simple model was set up to predict estrogen concentrations and endocrine disruption risk in the Yodo River, Japan. This catchment spans the conurbations of Kyoto and Osaka and is the main source of drinking water for Osaka City, Japan. From the river survey data (5 separate occasions between 2005 and 2008), a maximum of 32 g per day estrone (E1)...

Water reclamation projects are established all over the world to lighten the water stress or scarcity problem. For choosing water reclamation processes not only water qualities but also energy consumption should be considered. This study evaluated the potential of ozonation and ceramic membrane filtration (CMF) combination processes application for...

The rapid increase in the production and use of fullerene C(60) nanoparticles raise concerns about environmental risks and human health. Wastewater treatment plants are key barriers to their discharge into the environment. The aggregation behavior of aqueous suspensions of C(60) nanoparticles (nC(60)) could affect their transport, bioavailability,...

Time-dependent dynamics in the concentrations of four anti-influenza drugs (oseltamivir, oseltamivir carboxylate, zanamivir, and amantadine) in environmental waters collected from the Yodo River basin, Japan, were monitored for the first time over a 1-year period (July 2010 to June 2011). The clear, convex dynamic profiles of oseltamivir, oseltamiv...

We developed a methodology for the separation, identification, and quantification of eight N-nitrosamines. For a range of wastewater samples, including raw sewage and final-discharge wastewater, the methodology, which was based on solid-phase extraction (SPE) and a purification technique followed by analysis using a gas chromatograph equipped with...

Acute and chronic (or sub-chronic) toxicity of five selected antimicrobial agents, including triclosan (TCS), triclocarban (TCC), resorcinol, phenoxyethanol and p-thymol, was investigated using the conventional three-aquatic-organism battery. These compounds are widely used in cosmetics and other personal care products and their ecological risk has...

The increasing production and use of fullerene nanomaterials raised their exposure potential to the activated sludge during biological wastewater treatment process. In this study, the toxicity of aqueous nanoscaled C60 (nC60) to activated sludge was investigated using nitrification inhibition and Microtox test. The test solutions of nC60 were prepa...

The fate and behavior of estrone-3-sulfate (E1-3S), estradiol-3-sulfate (E2-3S), estrone-3-glucuronide (E1-3G) and estradiol-3-glucuronide (E2-3G) were studied in raw sewage, activated sludge and river water using microcosms. The glucuronide conjugates had a half-life of 0.4h in raw sewage, yielding 40-60% of their free estrogens. Field observation...

We report the results of a nationwide survey of commonly used human and veterinary antibiotics (7 sulfonamides, trimethoprim, and 4 macrolides) in 37 Japanese rivers. Concentrations of the sum of the 12 target antibiotics ranged from undetectable to 626 ng/L, with a median of 7.3 ng/L for the 37 rivers. Antibiotics concentrations were higher in urb...

The ubiquitous application and release of antibiotics to the environment can result in bacterial antibiotic resistance, which in turn can be a serious risk to humans and other animals. Southeast Asian countries commonly apply an integrated recycling farm system called VAC (Vegetable, Aquaculture and Caged animal). In the VAC environment, antibiotic...

N-Nitrosodimethylamine (NDMA) is a potent carcinogen that is formed during disinfection by chlorination or ozonation in wastewater treatment plants (WWTPs). At present, little is known about the occurrence and fate of NDMA and its formation potential (FP) during wastewater treatment. We investigated the fate of NDMA and NDMA FP in 12 WWTPs. NDMA oc...

A detailed study of the free and conjugated estrogen load discharged by the eight major sewage treatment plants into the Yodo River basin, Japan was carried out. Sampling campaigns were focused on the winter and autumn seasons from 2005 to 2008 and the free estrogens estrone(E1), 17β-estradiol(E2), estriol(E3), 17α-ethynylestradiol(EE2) as well as...

The occurrence of free and conjugated estrogens was examined in a survey of eleven sewage treatment plants (STPs) and their discharge water in the United Kingdom using grab sampling. The STPs included trickling filter with and without tertiary treatment, and activated sludge with tertiary treatment. For three activated sludge plants both influent a...

We investigated the occurrence and fate of the two antiviral drugs oseltamivir carboxylate (OC)-the active metabolite of Tamiflu-and amantadine (AMT) at three sewage treatment plants (STPs) during the 2008-2009 and 2009-2010 influenza seasons in Japan. Both compounds were detected in all samples analyzed. The concentrations in raw influents at the...

The behavior of antibacterial triclosan, insect-repellent diethyltoluamide (DEET), anticonvulsant carbamazepine, and antipruritic crotamiton was investigated at two sewage treatment plants (STPs) to clarify their complete mass balance. Twenty-four-hour flow-proportional composite samples were collected from the influent and effluent of primary and...

Oseltamivir phosphate (OP; Tamiflu) is a prodrug of the anti-influenza neuraminidase inhibitor oseltamivir carboxylate (OC) and has been developed for the treatment and prevention of both A and B strains of influenza. The recent increase in OP resistance in influenza A virus (H1N1; commonly called "swine flu") has raised questions about the widespr...

This article describes the development of a short pre-treatment method that allows the simultaneous analysis of free estrogens (estrone, 17beta-estradiol, estriol and 17 alpha-ethynylestradiol) and their sulphate and glucuronide conjugated forms. For a range of matrices, from sewage effluent to river water, the developed methodology based on solid-...

  The objective of this study is to evaluate the effect of estrogens on fish endocrine disruption in river water and treated wastewater. Endogenous estrogen estrone (E1), 17β-estradiol (E2), river water, and treated wastewater were used for exposure tests on male Japanese medaka. Vitellogenin induction in male medaka was regarded as the endpoint of...

This paper describes the behavior, occurrence and de-conjugation rate of the conjugated estrogens in raw wastewater. De-conjugation of sulphate and glucuronide conjugates in raw wastewater was investigated in batch experiments (flasks shake). The experiments were carried out at 2.5 μg/L initial concentration. Both conjugates were transformed into t...

We examined the utility of 13 pharmaceuticals and personal care products (PPCPs) as molecular markers of sewage contamination in riverine, groundwater, and coastal environments. The PPCPs were crotamiton, ibuprofen, naproxen, ketoprofen, fenoprofen, mefenamic acid, thymol, triclosan, propyphenazone, carbamazepine, diethyltoluamide, ethenzamide, and...

Groundwater replenishment by infiltration of road runoff is expected to be a promising option for ensuring a sustainable urban water cycle. In this study, we performed a soil infiltration column test using artificial road runoff equivalent to approximately 11-12 years of rainfall to evaluate the removal of pollutants by using various chemical analy...

The existence of pharmaceuticals and personal care products (PPCPs) in the water environment is an emerging problem. In this study, we investigated the toxicity of eleven PPCPs through bioassays on bacteria, algae, crustaceans, amphibians and protozoa, and compared the toxicology indexes with the concentration of PPCPs in river water for ecotoxiclo...

We investigated the efficiencies of removal of 24 pharmaceutically active compounds (PhACs) during sand filtration and ozonation in an operating municipal sewage treatment plant (STP). The target compounds were 2 phenolic antiseptics (thymol, triclosan), 5 acidic analgesics or anti-inflammatories (ibuprofen, naproxen, ketoprofen, fenoprofen, mefena...

Ten wastewater treatment plants were surveyed to clarify the occurrence and fate of pharmaceuticals and personal care products (PPCPs) in wastewater systems in Japan. It was found that the concentration of most PPCPs in influent wastewater ranged from hundreds to one thousand ng/L and that several patterns of PPCP removal in the treatment process e...

The occurrence of 70 pharmaceutical and personal care products (PPCPs) was investigated in the Tone River. The river has the largest basin in Japan, and the water is utilized not only for farming, but also as a source of water supply. One day in both January and October 2006, surface waters in the river and its tributaries and effluents from sewage...

In recent years, pharmaceuticals have attracted attention as new environmental contaminants. Their detections in the aqueous environment thanks to innovative analytical methods have been extensively reported in Europe and North America. The purpose of this study is to develop an innovative and timesaving method to analyze many pharmaceuticals in wa...