Norbert Gottschlich

• Account Manager at thinxxs Microtechnology AG

We present a novel, cost-efficient process chain for fast tooling and small-lot replication of high-quality, multi-scale microfluidic polymer chips within less than 5 days. The fabrication chain starts with a primary master which is made by well-established cleanroom processes such as DRIE or negative SU-8 resist based surface micromachining. The f...

Erster Schritt bei der Strukturaufklärung von Proteinen mit Röntgenstrukturanalyse ist die Züchtung von Proteinkristallen. Hochdurchsatz-Techniken sowie spezialisierte Mikroplatten tragen entscheidend dazu bei, sowohl den zeitlichen als auch den finanziellen Aufwand zu reduzieren.

Complementing single electrode recordings, passive, substrate-integrated thin-film microelectrode arrays (MEAs) have become established tools to investigate spatio-temporal patterns of electrical activity and neuronal interaction in-vitro. In the neurosciences, acute brain slices with accessible and well-preserved neuronal microcircuitry have becom...

In the future, plastic-based lab-on-a-chip systems will play a crucial role in modern life sciences, e.g. biotechnology and (bio)medical engineering. Presently, microfluidic systems for capillary electrophoresis (CE) are being used. They allow for the safe handling of smallest substance volumes in the pL range and their separation into the individu...

The detection of ionic species in a polymeric planar electrophoresis device by contactless conductivity measurement is described. To our knowledge this is the first report of such measurements carried out with external electrodes which are part of the holder rather than the separation chip itself. The approach allows the use of bare devices as used...

In today's biomedical research and diagnosis, a number of substances and agents have to be checked. Frequently, plastic micro titer plates are used for this purpose as large-area test platforms. For the first time, plastic micro titer plates with 96 identical microfluidic labon-a-chip structures for simultaneous capillary electrophoresis (CE) have...

A two-dimensional separation system on a microfabricated device was demonstrated using open-channel electrochromatography as the first dimension and capillary electrophoresis as the second dimension. The first dimension was operated under isocratic conditions, and the effluent from the first dimension was repetitively injected into the second dimen...

A microchip device was demonstrated that integrated enzymatic reactions, electrophoretic separation of the reactants from the products and post-separation labeling of proteins and peptides prior to detection. A tryptic digestion of oxidized insulin B-chain was performed in 15 min under stopped flow conditions in a heated channel, and the separation...

A simulated moving bed (SMB) system has been developed for the biospecific purification of monoclonal antibodies. Adsorption and desorption of the desired product is performed under different conditions. To increase the purity and yield of the antibodies, two purge steps have to be introduced. The steady-state performance of the SMB system was mode...

A system for continuous biospecific affinity purification of enzymes by simulated moving-bed chromatography was designed. The performance of steady-state operation was predicted by a model based on a linear driving force approximation. The system was tested for the purification of bovine α-chymotrypsin on immobilized soybean trypsin inhibitor. The...

Several monoclonal antibodies (mABs) have been prepared and immobilized for the biospecific isolation of penicillin amidase (PA) from Escherichia coli (EC 3.5.1.11), an enzyme without S-S bridges and a pH stability range of 4-9. During the immobilization the fluorescence emission maxima of the mABs were found to change from 336 nm to ca. 350 nm. On...

Zugl.: Hamburg-Harburg, Techn. Universiẗat, Diss., 1997.