Nora Ventosa

Nora Ventosa
Materials Science Institute of Barcelona · Molecular nanoscience and organic materials

About

147
Publications
12,033
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2,881
Citations
Citations since 2016
45 Research Items
1467 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
Introduction
I’m research scientist at the Institute of Materials Science of Barcelona (ICMAB) of CSIC. In my lab, we do research on green chemistry, soft materials and nanobiochemistry. We investigate, develop and apply new methodologies, based on compressed fluids, in order to gain control of molecular self-assembly in solution. Furthermore, we also focus our research work on particulate soft materials for drug delivery. We are currently working on: 1) Fine structural tuning of peptide targeted liposomes for enzyme delivery. 2) Quatsomes: new highly stable nanovesicles as nanocarriers for drug delivery. 3) Surfactant-free pressure tunnable microemulsions

Publications

Publications (147)
Article
Full-text available
Fluorescent organic nanoparticles (FONs) are a large family of nanostructures constituted by organic components that emit light in different spectral regions upon excitation, due to the presence of organic fluorophores. FONs are of great interest for numerous biological and medical applications, due to their high tunability in terms of composition,...
Article
Full-text available
The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on...
Article
Hypothesis Quatsome nanovesicles, formed through the self-assembly of cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) in water, have shown long-term stability in terms of size and morphology, while at the same time exhibiting high CHOL-CTAB intermolecular binding energies. We hypothesize that CHOL/CTAB quatsomes are indeed thermodynami...
Article
Lysosomal storage disorders (LSD) are a group of rare life-threatening diseases caused by a lysosomal dysfunction, usually due to the lack of a single enzyme required for the metabolism of macromolecules, which leads to a lysosomal accumulation of specific substrates, resulting in severe disease manifestations and early death. There is currently no...
Article
Full-text available
The development of contrast agents based on fluorescent nanoparticles with high brightness and stability is a key factor to improve the resolution and signal-to-noise ratio of current fluorescence imaging techniques. However, the design of bright fluorescent nanoparticles remains challenging due to fluorescence self-quenching at high concentrations...
Article
Full-text available
The efficacy of the treatment of bacterial infection is seriously reduced because of antibiotic resistance; thus, therapeutic solutions against drug-resistant microbes are necessary. Nanoparticle-based solutions are particularly promising for meeting this challenge because they can offer intrinsic antimicrobial activity and sustained drug release a...
Chapter
The processing of inclusion bodies (IBs) into surfaces is of great interest for cell culture applications due to the combined physical and biological cues these particles provide. The arrangement of these IBs into defined and tunable micropatterns can be useful for basic research purposes regarding the mechanical properties needed for cell adhesion...
Chapter
The physicochemical characterization of protein aggregates yields an important contribution to further our understanding on many diseases for which the formation of protein aggregates is one of the pathological hallmarks. On the other hand, bacterial inclusion bodies (IBs) have recently been shown to be highly pure proteinaceous aggregates of a few...
Article
Full-text available
Topical delivery has received great attention due to its localized drug delivery, its patient compliance, and its low risk for side effects. Recent developments have focused on studying new drug delivery systems as a strategy for addressing the challenges of current topical treatments. Here we describe the advances on an innovative drug delivery pl...
Article
Full-text available
The development of artificial vesicles into responsive architectures capable of sensing the biological environment and simultaneously signaling the presence of a specific target molecule is a key challenge in a range of biomedical applications from drug delivery to diagnostic tools. Herein, the rational design of biomimetic DNA‐grafted quatsome (QS...
Article
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MicroRNAs (miRNAs) are small non‐coding endogenous RNAs, which are attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited due to their unstability in the bloodstream, caused by nucleases abundantly present in the blood, and poor capa...
Article
Full-text available
Fabricating polymeric scaffolds using cost-effective manufacturing processes is still challenging. Gas foaming techniques using supercritical carbon dioxide (scCO2) have attracted attention for producing synthetic polymer matrices; however, the high-pressure requirements are often a technological barrier for its widespread use. Compressed 1,1,1,2-t...
Article
We present a comprehensive study of two indocarbocyanines, DiI (1,1 0-dioctadecyl-3,3,3 0 3 0-tetra-methylindocarbocyanine perchlorate) and DiD (1,1 0-dioctadecyl-3,3,3 0 ,3 0-tetramethylindodicarbocyanine perchlorate), in water suspensions in the presence of a CTAB surfactant both above and below the critical micellar concentration. The very good...
Article
In article number 2000260, Jaume Veciana, Nora Ventosa and co‐workers report a soft, reliable, and scalable method based on compressed CO2 for obtaining nanoconjugates of recombinant human epidermal growth factors and nanovesicles. These nanoconjugates exhibit appropriate physicochemical properties together with an antimicrobial activity preventing...
Article
The administration of hydrophobic actives and drugs for medical or cosmetical purposes generally requires a formulation that ensures adequate water solubility, which can be achieved through the encapsulation in liposomes. For the vehiculation of (+)-usnic acid (UA), a hydrophobic compound with antioxidant activity, we have prepared liposomes in a o...
Article
We found a quasi-incompressible and liquid mixture of ibuprofen, water and acetone, that enables the crystallization of ibuprofen particles by decreasing the pressure from 15MPa to ambient pressure (~0.1MPa). We measured the solubility of ibuprofen in the mixture as a function of the acetone/water-ratio for pressures of 0.1MPa, 5.5MPa and 15MPa and...
Article
Full-text available
A multitude of microparticles and nanoparticles is developed to improve the delivery of different small drugs and large biomolecules, which are subject to several hindering biological barriers that limit their optimal biodistribution and therapeutic effects. Here, a soft, reliable, and scalable method based on compressed CO2 is reported for obtaini...
Article
Fabry disease is a rare lysosomal storage disorder characterized by a deficiency of α-galactosidase A (GLA), a lysosomal hydrolase. The enzyme replacement therapy administering naked GLA shows several drawbacks including poor biodistribution, limited efficacy, and relatively high immunogenicity in Fabry patients. An attractive strategy to overcome...
Article
Full-text available
Fabry disease is a lysosomal storage disease arising from a deficiency of the enzyme α-galactosidase A (GLA). The enzyme deficiency results in an accumulation of glycolipids, which over time, leads to cardiovascular, cerebrovascular, and renal disease, ultimately leading to death in the fourth or fifth decade of life. Currently, lysosomal storage d...
Article
In tissue engineering, biological, physical, and chemical inputs have to be combined to properly mimic cellular environments and successfully build artificial tissues, which can be designed to fulfil different biomedical needs such as organ donor shortage or the development of in vitro disease models for drug testing. Inclusion body-like protein na...
Article
Full-text available
Fluorescent organic nanoparticles (FONs) are emerging as an attractive alternative to the well-stablished fluorescent inorganic nanoparticles or small organic dyes. Their proper design allows one to obtain biocompatible probes with superior brightness and high photostability, although usually affected by low colloidal stability. Herein, we present...
Article
Quatsomes are outstanding new lipid-based nanovesicles that are highly homogeneous and stable in different media for years, but the composition must be carefully chosen to avoid any potentially toxic side effects in in vivo applications. To this end, we have developed and studied a novel type of Quatsomes composed of cholesterol and myristalkonium...
Article
The influence of the presence of synthetic structurally related N-oxide and corresponding cationic surfactants with different chain lengths on the properties of 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomes was investigated. Their potentiality as delivery systems of (+)-usnic acid was also evaluated by studying its entrapment efficiency and...
Article
Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enab...
Article
Full-text available
We present a fast optical screening method for the detection of sub-micro-structures nanostructuration in water-containing fluids. The optical method is based on Raman spectroscopy of the water stretching vibration. The shape of this Raman band reveals information about the development of hydrogen bonds, from which it can be extracted whether the w...
Article
Full-text available
Correction for ‘Pulling lipid tubes from supported bilayers unveils the underlying substrate contribution to the membrane mechanics’ by Marina I. Giannotti et al. , Nanoscale , 2018, 10 , 14763–14770.
Article
Quatsomes (QS) are unilamellar nanovesicles constituted by quaternary ammonium surfactants and sterols in defined molar ratios. Unlike conventional liposomes, QS are stable upon long storage such as for several years, they show outstanding vesicle to vesicle homogeneity regarding size and lamellarity, and they have the structural and physicochemica...
Article
Polyethyleneimine (PEI) has been used extensively for transient gene expression (TGE) in mammalian cell cultures. However, the relationship between DNA/PEI complex preparation and their biological activity has not been fully established. Here, a systematic study of DNA/PEI complexes, their physicochemical properties during formation and their trans...
Poster
Nanomedicine development has improved over traditional approaches as new nanomaterials emerge at the nano-scale. In addition to favorable pharmacokinetics, which improves bioavailability, solubility and therapeutic index of their payload, nano-scaled pharmaceuticals can offer controlled release at the disease site. The H2020 Project Smart-4-Fabry a...
Article
Full-text available
Cell processes like endocytosis, membrane resealing, signaling and transcription, involve conformational changes which depend on the chemical composition and the physicochemical properties of the lipid membrane. The better understanding of the mechanical role of the lipids in cell membrane force-triggered and sensing mechanisms has recently become...
Article
Full-text available
Diketopyrrolopyrroles (DPPs) have recently attracted large interest as highly bright and photostable red‐emitting molecules. However, their tendency to form non‐fluorescent aggregates in water via the so‐called Aggregation Caused Quenching (ACQ) effect is a major issue that limits their application under the microscope. In this work, two DPP molecu...
Article
A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate (DiI), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indodicarbocyanine perchlorate (D...
Article
Fluorescent silicon (Si) nanocrystals (2.8 nm diameter) were incorporated into surfactant assemblies of cetyl trimethylammonium bromide (CTAB) and cholesterol, called quatsomes. In water, the quatsome-Si nanocrystal assemblies remain fluorescent and well-dispersed for weeks. In contrast to Si nanocrystals, alkanethiol-capped gold (Au) nanocrystals...
Article
Full-text available
This article describes the characterization of various encapsulated formulations of benznidazole, the current first-line drug for the treatment of Chagas disease. Given the adverse effects of benznidazole, safer formulations of this drug have a great interest. In fact, treatment of Chagas disease with benznidazole has to be discontinued in as much...
Article
Full-text available
Microemulsions are extensively used in advanced material and chemical processing. However, considerable amounts of surfactant are needed for their formulation, which is a drawback due to both economic and ecological reasons. Here, we describe the nanostructuration of recently discovered surfactant-free, carbon dioxide (CO2)-based microemulsion-like...
Article
Full-text available
Delivery of hydrophobic materials in biological systems, for example, contrast agents or drugs, is an obdurate challenge, severely restricting the use of materials with otherwise advantageous properties. The synthesis and characterization of a highly stable and water-soluble nano-vesicle, referred to as a quatsome (QS, vesicle prepared from cholest...
Article
Molecular self-assembly has enabled the fabrication of biologically inspired, advanced nanostructures as lipid-based nanovesicles (L-NVs). The oldest L-NVs, liposomes, have been widely proposed as potential candidates for drug delivery, diagnostic and/or theranostic applications and some liposome-based drug products have already stepped from the la...
Article
1,2,3-Triazole rings bearing hydrophobic aliphatic or hydrophilic oligoether chains were easily introduced at the two ends of the conjugated skeleton of bisthiophene-diketopyrrolopyrrole (TDPP) derivatives by simple click cycloaddition reactions. The combination of side chains with different structures and polarities on the triazole rings with the...
Article
Full-text available
Lysosomal storage disorders (LSD) are caused by lysosomal dysfunction usually as a consequence of deficiency of a single enzyme required for the metabolism of macromolecules, such as lipids, glycoproteins, and mucopolysaccharides. For instance, the lack of α-galactosidase A (GLA) activity in Fabry disease patients causes the accumulation of glycosp...
Article
Physicochemical characterization of protein aggregates is important on one hand, due to its large impact in understanding many diseases for which formation of protein aggregates is one of the pathological hallmarks. On the other hand, recently it has been observed that bacterial inclusion bodies (IBs) are also highly pure proteinaceous aggregates o...
Chapter
Compressed fluids and especially CO2-expanded solvents (a mixed solvent composed of CO2 dissolved in an organic solvent) present unique properties for the eco-efficient production of active pharmaceutical ingredients (APIs) with an exceptional control of the operational variables that allow tuning the final properties of the active compounds. The p...
Article
Full-text available
Growth hormone releasing peptide, GHRP-6, a hexapeptide (His-(D-Trp)-Ala-Trp-(D-Phe)-Lys-NH2, MW = 872.44 Da) that belongs to a class of synthetic growth hormone secretagogues, can stimulate growth hormone secretion from somatotrophs in several species including humans. In the present study, we demonstrate that GHRP-6 dispersed in aqueous solution,...
Article
Full-text available
The presence of water-rich and water-lean nanodomains in a transparent, pressurized "water-acetone-CO2" mixture was revealed by Raman spectroscopy. This nano-structured liquid can be classified as a surfactant-free microemulsion-like system and has the capacity to dissolve hydrophobic compounds, such as ibuprofen, in the presence of large amounts o...
Patent
Full-text available
The invention provides a novel method for obtaining solid micro- or nanoparticles with a homogeneous structure. A method is provided for obtaining solid micro- or nanoparticles with a homogeneous structure having a particle size of less than 10 μm where the processed solid compound has the natural, crystalline, amorphous, polymorphic and other feat...
Article
Phytosterols have been receiving increasing attention due to their demonstrated health benefits. Micronization of phytosterol particles is desirable to enhance their physiological efficacy. Utilization of the environmentally friendly compressed fluid-based technology, called Depressurization of an Expanded Liquid Organic Solution (DELOS) was invest...
Article
Nanomedicine, as the medical application of Nanotechnology, is a promising alternative to overcome the problems of the administration of APIs and biomolecules, either already existing drugs or new entities coming out from the discovery pipeline. In this paper we endeavour to show the potentiality of DELOS Technology Platform, which uses CO2-expande...
Article
Integration of therapeutic biomolecules, such as proteins and peptides, in nanovesicles is a widely used strategy to improve their stability and efficacy. However, translation of these promising nanotherapeutics to clinical tests is still challenged by the complexity involved in the preparation of functional nanovesicles, their reproducibility, sca...
Article
Appropriate combinations of mechanical and biological stimuli are required to promote proper colonization of substrate materials in regenerative medicine. In this context, 3D scaffolds formed by compatible and biodegradable materials are under continuous development in an attempt to mimic the extracellular environment of mammalian cells. We have he...
Article
Cells responses, like positioning, morphological changes, proliferation and apoptosis are the result of complex chemical, topographical and biological stimuli. Here we show the macroscopic responses of cells when nanoscale profiles made with Inclusion Bodies (IBs) are used for the 2D engineering of biological interfaces at the microscale. A deep st...
Article
Thermodynamically stable nanovesicular structures are of high interest for academia and industry in a wide variety of application fields, ranging from nanomaterials preparation to nanomedicine. Here we show the ability of quaternary ammonium surfactants and sterols to self-assemble forming stable amphiphilic bimolecular building-blocks with the app...
Article
Full-text available
The clinical management of human brucellosis is still challenging and demands in vitro active antibiotics capable of targeting the pathogen-harboring intracellular compartments. A sustained release of the antibiotic at the site of infection would make it possible to reduce the number of required doses and thus the treatment-associated toxicity. In...
Article
Slow protein release from amyloidal materials is a molecular platform used by nature to control protein hormone secretion in the endocrine system. The molecular mechanics of the sustained protein release from amyloids remains essentially unexplored. Inclusion bodies (IBs) are natural amyloids that occur as discrete protein nanoparticles in recombin...
Article
The structuring of synthetic and biological therapeutic actives as micro- and nano-particulate materials is a widely accepted formulation strategy to improve efficacy and reduce the toxicity of drugs. However, the development of efficient production platforms that enable the formulation of these nanomedicines at an industrial scale and with the qua...