Niti SharmaGachon University | kyungwon · Gachon BioNano Research Center
Niti Sharma
PhD
Gachon University, South Korea
About
63
Publications
17,066
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1,458
Citations
Citations since 2017
Introduction
Neurodegenerative diseases, Alzheimer's disease, Plant extracts, Cell culture, Enzymes, Mode of action
Additional affiliations
August 2016 - June 2021
Patanjali Research Foundation
Position
- Scientis F
October 2014 - April 2016
Independent Researcher
Position
- Senior Researcher
October 2010 - July 2014
Publications
Publications (63)
Neurodegenerative diseases (NDs) are a family of disorders that cause progressive structural and functional degeneration of neurons. Among all the organs in the body, the brain is the one that is the most affected by the production and accumulation of ROS. Various studies have shown that an increase in oxidative stress is a common pathophysiology f...
Alzheimer's disease (AD) is a neurodegenerative disease that causes a gradual loss of normal motor and cognitive function. The complex AD pathophysiology involves various factors such as oxidative stress, neuroinflammation, amyloid-beta (Aβ) aggregation, disturbed neurotransmis-sion, and apoptosis. The available drugs suffer from a range of side ef...
Prion diseases are a group of incurable zoonotic neurodegenerative diseases (NDDs) in humans and other animals caused by the prion proteins. The abnormal folding and aggregation of the soluble cellular prion proteins (PrPC) into scrapie isoform (PrPSc) in the Central nervous system (CNS) resulted in brain damage and other neurological symptoms. Dif...
Prion diseases are a group of incurable zoonotic neurodegenerative diseases (NDDs) in humans and other animals caused by the prion proteins. The abnormal folding and aggregation of the soluble cellular prion proteins (PrP C) into scrapie isoform (PrP Sc) in the Central nervous system (CNS) resulted in brain damage and other neurological symptoms. D...
Ficus religiosa (Bo tree or sacred fig) and Ficus benghalensis (Indian banyan) are of immense spiritual and therapeutic importance. Various parts of these trees have been investigated for their antioxidant, antimicrobial, anticonvulsant, antidiabetic, anti-inflammatory, analgesic, hepatopro-tective, dermoprotective, and nephroprotective properties....
Prion diseases are a group of incurable neurodegenerative
diseases caused by the prions, affecting both
humans and animals. The atypical folding and aggregation
of the soluble cellular prion proteins (PrPC) into
scrapie isoform (PrPSc) in the CNS, results in brain
damage and other symptoms associated with it.
Different therapeutic approaches rangin...
Tropane alkaloids (TAs) are among the most valued chemical compounds known since pre-historic times. Poisonous plants from Solanaceae family ( Hyoscyamus niger, Datura, Atropa belladonna , Scopolia lurida, Mandragora officinarum, Duboisia ) and Erythroxylaceae ( Erythroxylum coca ) are rich sources of tropane alkaloids. These compounds possess the...
Plant-derived (phyto) carbazole alkaloids are an important class of compounds, presented in the family of Rutaceae (Genera Murraya, Clausena, Glycosmis, Micromelum and Zanthoxylum). Due to several significant biological activities, such as antitumor, antibacterial, antiviral, antidiabetic, anti-HIV and neuroprotective activities of the parent skele...
Neurodegenerative diseases (NDs) mainly affect neurons and gradually lead to a loss of normal motor and cognitive functions. Atypical protein homeostasis-misfolding, aggregations and accumulations, oxidative stress, inflammation, and apoptosis-are common features in most NDs. To date, due to the complex etiology and pathogenesis of NDs, no defined...
Phytosterols constitute a class of natural products that are an important component of diet and have vast applications in foods, cosmetics, and herbal medicines. With many and diverse isolated structures in nature, they exhibit a broad range of biological and pharmacological activities. Among over 200 types of phytosterols, stigmasterol and β-sitos...
Alzheimer's disease (AD) is one of the most prevalent neurodegenerative diseases worldwide. In an effort to search for new strategies for treating AD, natural products have become candidates of choice. Plants are a rich source of bioactive and effective compounds used in treating numerous diseases. Various plant extracts are known to display neurop...
Purpose:
Asthma is a heterogeneous airway inflammatory disease with limited therapeutic options. Traditional medicine is extensively used for treating various ailments including asthma. Sahastraputi-Abhrak-Bhasma (SPAB) is a biotite-calx based Indian medicine.
Methods:
We have tested for the anti-inflammatory and anti-asthmatic properties of SPA...
Identification of novel anti-inflammatory strategies are needed to avoid the side effects associated with the currently available therapies. Use of anti-inflammatory herbal remedies is gaining attention. The purpose of the present investigation was to evaluate the pharmacological potential of the withanolide-rich root extracts of the medical plant...
To identify novel potent cardiac myosin activator, a series of diphenylalkylisoxazol-5-amine compounds 4–7 have been synthesized and evaluated for cardiac myosin ATPase activation. Among the 37 compounds, 4a (CMA at 10 µM = 81.6%), 4w (CMA at 10 µM = 71.2%) and 6b (CMA at 10 µM = 67.4%) showed potent cardiac myosin activation at a single concentrat...
The widely used cancer treatment, chemotherapy, causes severe long-term neuropathic pain in 30-40% cases, the condition clinically known as chemotherapy-induced peripheral neuropathy (CIPN). Approved conventional analgesics are sometimes ineffective, while others like opioids have undesirable side effects like addiction, seizures, and respiratory m...
Oroxilum indicum has an extensive history in the Indian traditional medicine system. The root and stem bark of this plant is well accepted for the treatment, prevention and cure of various types of diseases. The bark of the plant has a high content of flavonoids and other phenolics, which are known to be responsible for exhibiting various therapeut...
Background and Purpose
: Asthma is a chronic respiratory disease orchestrated by immune and structural cells. Identification of novel therapeutic strategies are needed for asthma due to the limitations of existing therapies. We have validated the anti-inflammatory, anti-asthmatic and immunomodulatory therapeutic properties of herbal decoction, Divy...
Asthma is a chronic allergic respiratory disease with limited therapeutic options. Here we validated the potential anti-inflammatory, anti-asthmatic and immunomodulatory therapeutic properties of calcio-herbal ayurvedic formulation, Divya-Swasari-Ras (DSR) in-vivo, using mouse model of ovalbumin (OVA) induced allergic asthma. HPLC analysis identifi...
The incidence rate of diabetes mellitus is increasing worldwide. Herbal formulations have recently gained importance as an alternative therapeutic option in controlling diabetes without causing any side effects In the present study, we have demonstrated maintenance of glycemic homeostasis and anti-adipogenic potential of a herbo-mineral formulation...
Bhasmas are Ayurvedic herbo-mineral formulations that have been used since ancient times for therapeutic benefits. Godanti Bhasma (GB) is an anhydrous calcium sulfate preparation processed by heating of gypsum powder with herbal extracts. Thermo-transformation of gypsum into the anhydrous GB was confirmed by Raman and FT-IR spectroscopy. GB particl...
(1) Background: Withania somnifera Dunal (Ashwagandha) is a widely used medicinal herb in traditional medicinal systems with extensive research on various plant parts. Surprisingly, seeds of W. somnifera have never been investigated for their therapeutic potential. (2) Methods: W. somnifera seeds were extracted for fatty acids (WSSO) using super cr...
Introduction:
A comparative study of Putranjiva roxburghii Wall. seed extract and developed silver nanoparticles (PJSNPs) for improving bioavailability that enhance their anti-cancer activity against HCT-116 (colon carcinoma), PANC-1 (pancreatic carcinoma), MDA-MB 231 (breast carcinoma) cell lines was performed.
Materials and methods:
The green...
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Colchicine was extracted from Gloriosa superba seeds using the Super Critical Fluid (CO2) Extraction (SCFE) technology. The seeds were purified upto 99.82% using column chromatography. Colchicine affinity was further investigated for anticancer activity in six human cancer cell lines, i.e., A549, MCF-7, MDA-MB231, PANC-1, HCT116, and SiHa. Purified...
The sulfonamidophenylethylamide analogues were explored for finding novel and potent cardiac myosin activators. Among them, N-(4-(N,N-dimethylsulfamoyl)phenethyl-N-methyl-5-phenylpentanamide (13, CMA at 10 µM = 48.5%; FS = 26.21%; EF = 15.28%) and its isomer, 4-(4-(N,N-dimethylsulfamoyl)phenyl-N-methyl-N-(3-phenylpropyl)butanamide (27, CMA at 10 µM...
Ethnopharmacological relevance
Peedantak Vati (PV) is a polyherbal ayurvedic formulation, which is regularly prescribed by the ayurvedic practitioner for the inflammatory disorders and joints pain in India. It is composed of 23 different herbs and minerals, described in ayurvedic text for their anti-inflammatory and analgesic properties.
Aim of th...
![Figure 1: Position of Chakras in Human Body [54].](publication/356204662/figure/fig1/AS:1090419735699458@1636987732782/Position-of-Chakras-in-Human-Body-54_Q320.jpg)
Astavarga is nature's most extravagant group of flowering plants found in Himalayan region. As the name Astavarga
indicates, it contains eight medicinal plants belonging to Zingiberaceae, Orchidaceae and Liliaceae family viz, Kakoli,
Kshirakakoli, Meda, Mahameda, Jeevak, Risbhaka, Riddhi and Vridddhi. As per Ayurveda this group of medicinal plants...
To explore novel cardiac myosin activator, a series of diphenylalkyl substituted 1,3,4-oxadiazoles and 1,2,4-oxadiazoles have been prepared and tested for cardiac myosin ATPase activation in vitro. In all cases, three carbon spacer between the oxadiazole core and one of the phenyl ring was considered crucial. In case of 1,3,4-oxadiazole, zero to tw...
Green synthesis of silver nanoparticles (AgNPs) using Shivlingi (Bryonia laciniosa) seed extract was carried out.
Characterisation of synthesised nanoparticles was accomplished through the optical absorption and photoluminescence
spectrum, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier
t...
To optimize the lead urea scaffold 1 and 2 as selective cardiac myosin ATPase activator, a series of urea derivatives have been synthesized to explore its structure activity relationship. Among them N,N-dimethyl-4-(2-(3-(3-phenylpropyl)ureido)ethyl)benzenesulfonamide (13, CMA = 91.6%, FS = 17.62%; EF = 11.55%), N,N-dimethyl-4-(2-(1-methyl-3-(3-phen...
Background: Picrorhiza kurroa is an important medicinal plant in the traditional Chinese
Introduction: The Picrorhiza kurroa rhizome has a long history of use in Indian Ayurvedic and Chinese system of medicine for the treatment of a wide spectrum of diseases. Today it is viewed as an important therapeutic target in both Western and Eastern medicinal systems. This work was aimed to study the clastogenic effect of Picrorhiza kurroa rhizo...
Picrorhiza kurroa is a well-known ayurvedic or herbal medicine which is used very commonly in the treatment of various diseases. Therefore, we studied the oral toxicity of Picrorhiza kurroa rhizome extract in rats. A single high dose of the extract at 2000 mg/kg body weight was tested on Wistar rats. Mortality/viability and clinical signs were reco...
Ergot of Rye is a plant disease caused by the fungus Claviceps purpurea which infects the grains of cereals and grasses but it is being used for ages for its medicinal properties. All the naturally
obtained ergot alkaloids contain tetracyclic ergoline ring system, which makes them structurally similar with other neurotransmitters such as noradrenal...
Chrysosplenol C (4’,5,6-trihydroxy-3,3’,7-trimethoxyflavone) isolated from Miliusa balansae has unique structural features as a reversible inotropic agent independent of -adrenergic signaling and with selective activation of cardiac myosin ATPase. Hence, a series of chrysosplenol analogs were synthesized and explored for identification of pharmaco...
Water solubility of title compound (I) is remarkably increased by modifying the structure to the succinate (VIa) and pentanedioate (VIb).
To improve the water solubility of arylsulfonylimidazolidinone (JSH-2282), a potent anti-cancer agent, two urea derivatives, sodium (S)-2-(3-(4-(5-((S)-2-oxo-4-phenylimidazolidin-1-ylsulfonyl)indoline-1-carbonyl)phenyl)ureido)succinate (2a) and sodium (5)-2-(3-(4-(5-((S)-2-oxo-4-phenylimidazolidin-1-ylsulfonyl)indoline-1-carbonyl)phenyl)ureido)pent...
Based on the hits, 3,4-dihydroquinazoline-2-thione (1) and benzimidazole-2-thione (2), a series of indole-2-thione (3) and indole-2-thiol inhibitors (4) of melanogenesis were designed, synthesized and evaluated in melanoma B16 cells under the stimulant of α-melanocyte stimulating hormone (α-MSH). The indole-2-thione compounds (3a-g) exhibited an ef...
To investigate the possible isosteric replacement of imidazolidinone moiety in 4-phenyl-N-arylsulfonylimidazolidinone for broad and potent anticancer agents, a series of 4-phenyl-l(N)-arylsulfonylimidazolidinones 6a-k, imidazolidinethione analogs 7a-i, and imidazolidine oxime analogs 8a-c were prepared and evaluated for their in vitro anticancer ac...
Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and
important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18)
was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the pl...
In order to find new pancreatic lipase (triacylglycerol lipase, EC 3.1.1.3) inhibitors from natural sources, 75 medicinal plants belonging to different families were screened for their anti-lipase activity, using a radioactive method. On evaluating the results, methanolic extracts of three plants namely, Eriochloa villosa (Thunb.) Kunth, Orixa japo...
A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in
rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity
with an overall yield of 80.1% by ammonium sulfate precipitation and a single chromatographic step involv...
The inhibition of mushroom tyrosinase by methanolic extract of Dictyophora indusiata was evaluated and the bioactive component was characterized and identified as 5-(hydroxymethyl)-2-furfural (HMF) by chromatographic and spectroscopic means. Kinetic studies revealed it to be a noncompetitive inhibitor for the oxidation of L-DOPA. On the basis of th...
Tyrosinase, also known as polyphenol oxidase, is a copper-containing enzyme, which is widely distributed in microorganisms, animals, and plants. Nowadays mushroom tyrosinase has become popular because it is readily available and useful in a number of applications. This work presents a study on the importance of tyrosinase, especially that derived f...
An in vivo method of labelling lipid fractions in developing seeds of Brassica campestris using [1-14C] acetate has been developed. The “wick” method for introducing label into the intact plant is quite effective, safe and easy to use. The results obtained were reproducible and comparable to those reported earlier for seeds procured from greenhouse...
Dihydroxyacetone phosphate reductase (DHAP reductase) was purified to apparent electrophoretic homogeneity with about 24% recovery from immature seeds of Brassica campestris using (NH4)2SO4 fractionation, affinity chromatography, gel filtration and adsorption chromatography. The purified enzyme with molecular mass of about 62 kDa was a dimer with s...
Questions
Questions (3)
I have prepared a standard curve for Pyruvate for ALT and AST estimation in serum using manual method.
I need to convert Pyruvate (uM) to transaminase activity in U/L. I referred to literature and found tables that mention eg 1 uM=9U/L, 4 uM=18 U/L and so on. These values also varies from one protocol to other.
The standard curve is obtained by plotting the measured absorbances
Please suggest how to convert Pyruvate standard (uM) to enzyme activity (U/L).
Thanks
Please suggest the protocol for MMP 1 and MMP13 inhibitory assay (I'm not using the kit).
For beta glucuronidase inhibitory assay, I'm using Silymarin as positive control. However, it has solubility issue. I've prepared the Silymarin stock in DMSO(100%) . No ppt are observed after double dilution with buffer/water...but on subsequent dilutions ppt start appearing. I want to have [DMSO] less than 1 % in final assay system. The same condition is observed with Ethanol. Please suggest what can be done.
