About
8
Publications
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51
Citations
Introduction
Nitanshu Garg currently works at the Department of Molecular Biology and Biotechnology, The University of Sheffield. Nitanshu does research in Molecular Biology, Microbiology and Biotechnology. Their most recent publication is 'Bacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors'.
Additional affiliations
January 2015 - January 2019
Position
- PhD Student
Description
- Copper, Cytochromes and Campylobacter (1) Assembly of Cytochrome C Oxidase in Campylobacter Jejuni. (2) Copper Homeostasis in Campylobacter Jejuni. (3) Quinol-cytochromec reductase complex in Campylobacter Jejuni.
May 2013 - August 2013
Position
- Summer Research Intern
Description
- High Pressure EPR Reveals Conformational Equilibria and Volumetric Properties of Spin Labeled Proteins
Education
January 2015 - January 2019
July 2010 - May 2014
Publications
Publications (8)
Bacterial C-type haem-copper oxidases in the cbb3 family are widespread in microaerophiles, which exploit their high oxygen-binding affinity for growth in microoxic niches. In microaerophilic pathogens, C-type oxidases can be essential for infection, yet little is known about their biogenesis compared to model bacteria. Here, we have identified gen...
Abstract The periplasmic reduction of the electron acceptors nitrate (E m +420 mV) and trimethylamine-N-oxide (TMAO; E m +130 mV) by Nap and Tor reductases is widespread in Gram-negative bacteria and is usually considered to be driven by non-energy conserving quinol dehydrogenases. The Epsilonproteobacterium Campylobacter jejuni can grow by nitrate...
Fig. S1. Physiological data for the transition experiment from 150% to 40% aerobiosis. At time zero, the input gas composition was changed from 7.5% v/v oxygen (150% aerobiosis) to 1.88% oxygen (40% aerobiosis). A. Change in optical density at 600 nm. B. Corresponding increase in the specific acetate excretion rate. C. Dry weight at the high and lo...
Table S3. 2D‐gel analysis of proteins from cells grown at all steady‐states from 25% to 345% aerobiosis. In the ‘All steady‐state raw data’ tab, the normalised spot volumes from image analysis for a given protein are shown for multiple gels run with a minimum of 1 steady‐state per oxygen input condition (50% and 75% aerobiosis) or with two or more...
Table S1. Microarray data and RT‐PCR comparing the 150% and 40% aerobiosis steady states and at time points during the transition from 150% to 40% aerobiosis. In the ‘All significant (p < 0.05) genes’ tab, the fold‐changes of genes which showed a greater than twofold change in expression with a significance of p < 0.05 at any one of the time points...
Table S2. Label‐free LC‐MS/MS analysis of proteins at 40% and 150% aerobiosis. Samples from three independent steady‐states at 1.88% v/v oxygen or 40% aerobiosis (LF1‐3) and three at 7.5% v/v oxygen or 150% aerobiosis (LF‐4‐6) were analysed by LC‐MS/MS as described in Experimental Procedures. In the tables shown, A,B,C are gel lane replicates of ea...
Campylobacter jejuni, the most frequent cause of food-borne bacterial gastroenteritis worldwide, is a microaerophile that has to survive high environmental oxygen tensions, adapt to oxygen limitation in the intestine and resist host oxidative attack. Here, oxygen-dependent changes in C. jejuni physiology were studied at constant growth rate using c...
