Nishith Pal

Nishith Pal
West Bengal University of Health Sciences | WBUHS · Department of Microbiology

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101
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Publications

Publications (101)
Preprint
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This communication aims to validate the probiotic attributes of the Lactobacillus fermentum LMEM22 strain, safety profiling, 16S rRNA gene sequence analysis, and bacteriocin characterization. The bacteriocin of 13.1 kDa (based on the glycine-SDS-PAGE analysis) was isolated from L. fermentum LMEM22 curd strain (NCBI GenBank with accession No. MH3801...
Article
We report potentiation of healing efficacy of alginate by value addition at its structural level. Dual crosslinked (ionically and covalently) sodium alginate hydrogel coupled with honey (HSAG) brings about an intermediate stiffness in the fabric, confers consistent swelling property and limits erratic degradation of the polymer which ultimately pro...
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Background and objectives: The Score for Neonatal Acute Physiology II with Perinatal Extension (SNAPPE-II) is a vital tool for prognostication in newborns. The study was conducted with the hypothesis that the performance of the SNAPPE-II score might be affected by the presence of sepsis in newborns admitted with possible early onset septicemia and...
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Aims: To determine the spectrum of organisms with their sensitivity pattern and to explore whether the organisms isolated from inborn and outborn groups of neonates are identical or different. Methods: A total of 100 (50 inborn and 50 outborn) neonates were included in the study and 100 samples/swabs were taken from each site, viz axilla, umbilicus...
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The serum LDH (lactate dehydrogenase) levels have been reported to be increased in DF, and studies showed that LDH levels are higher in DHF and DSS cases [7], while an early elevation of LDH was recorded as an independent predictor of DHF [8]. As per the report of Perveen., et al. [9], at the time of hospital stay, patients having DHF had higher le...
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Human brucellosis is not an uncommon zoonotic disease worldwide with majority presenting with fever. Diagnostic difficulties, improper laboratory facilities and lack of knowledge about the disease are the important reasons for the disease to go unrecognized. The present study, between January 2015 to December 2015, identified cases of brucellosis a...
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Background: Indigenous lactic acid bacteria are well known probiotics having antibacterial activity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. Methods: Four curd samples were processed microbiologically for th...
Article
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Background Group A Streptococcus strains causing wide variety of diseases, recently became noticeable in eastern India, are not amenable to standard treatment protocol thus enhancing the possibility of disease morbidity by becoming antibiotic resistance. Methods The association of Lancefield group A Streptococcal variation with degree of vir archit...
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Aims: To assess the antioxidant and antibacterial activities of two locally available natural honey samples, and determine the total phenolic content (TPC) of the honeys. Methods: Antibacterial activity of two local natural honey samples (citrus honey: LNH1 and mango honey: LNH2) was determined against the clinical bacterial isolates of Staphylococ...
Article
Objective: The emergence of drug resistance has complicated tuberculosis (TB) scenario and is associated to treatment failure. The causative agent, Mycobacterium tuberculosis is usually slow growing and has been implicated as a contributing factor for drug tolerance and development of resistant strains. On the other hand, if rapidly growing bacilli...
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Secretary proteins of Mycobacterium tuberculosis are the key players of mycobacterial infection pathway. MTC28 is a 28kDa proline-rich secretary antigen of Mycobacterium tuberculosis and is only conserved in pathogenic strains of mycobacteria. Here we report the crystal structure of MTC28 at 2.8 and 2.15 angstrom resolutions for structure based epi...
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Technical limitations restrict routine anaerobe isolation from clinical materials in resource-limited laboratories. An innovative two steps combustion candle jar technique may be suitable for such setup. This system was tried with one case of chronic osteomyelitis developed on supracondyler compound fracture. Porphyromonas spp. was isolated and ide...
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Background: Some medical devices are reused after treatment with disinfectants at manufacturer′s recommended concentrations (RCs), based on experimental evaluation studies of their bactericidal, sporicidal, and mycobactericidal concentrations. However, this may not be sufficient to eliminate all colonized highly drug-resistant biofilms on medical d...
Article
Tuberculosis, caused by the bacteria Mycobacterium tuberculosis, is characterized by an infection in lung and spleen. In the present study, we have elucidated the mechanism by which Mycobacterium indicus pranii renders protection in in vivo Mycobacterium tuberculosis infection. We observed that Mycobacterium indicus pranii treated infected C57BL/6...
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Resistance to commonly used antibiotics by Enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. The present study was conducted to review the prevalence and identify therapeutic options for nosocomial Enterococcal infections in our tertiary care hospital. Isolates...
Conference Paper
TITLE: Medium modification for selecting Enterococcus spp. and Staphylococcus aureus from human stool. Authors: Chatterjee I, Chatterjee SS, Ghosh R, Mukherjee K, Saha S, Sammadar D, Ganguly S, Bhattacharya S, Pal NK. Institution: Microbiology, Nil Ratan Sircar Medical College & Hospital, Kolkata. ABSTRACT: Introduction: Staphylococcus aureus and...
Article
Tuberculosis causes severe immunosuppression thereby ensuring the loss of the host protective immune responses. During Mycobacterium tuberculosis infection, the pathogen modulates TLR-2 receptor down-stream signaling, indicating the possible involvement of TLR-2 in the regulation of the host immune response. Moreover, different PKC isoforms are als...
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Background Worldwide highest number of new pulmonary tuberculosis (PTB) cases, was reported from India in 2012. Adverse treatment outcomes and emergence of drug resistance further complicated the prevailing scenario owing to increased duration, cost and toxicity associated with the treatment of drug-resistant cases. Hence to reinforce India’s fight...
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AIMS: The emerging threat of widespread vancomycin resistance pose a serious public health concern. The objective of this study was to find the existence of vancomycin intermediate- resistant Staphylococcus spp (VIS) colonization in hands of health care workers. METHODS: 1284 isolates of Staphylococcus spp, from the fingers of 528 health care wo...
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Tuberculosis is characterized by severe immunosuppression of the host macrophages, resulting in the loss of the host protective immune responses. During Mycobacterium tuberculosis infection, the pathogen modulates C-C Chemokine Receptor 5 (CCR5) to enhance IL-10 production, indicating the possible involvement of CCR5 in regulation of the host immun...
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BACKGROUND: Current procedures for infection control in hospital environments have not been successful in curbing the rise in infections by multi-drug-resistant (MDR) pathogens. Emergence of resistance to chemical disinfectants is increasing steadily and has been reported worldwide. So prevention of multidrug-resistant health care associated inf...
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Context: Arthritis is inflammation of one or more joints. Terminalia chebula Retz. (Combretaceae) fruit is mentioned in Ayurveda as useful in treating arthritic disorders. Objective: This work was undertaken to evaluate the anti-inflammatory, antioxidant, anti-lipid peroxidative and membrane-stabilizing effects of hydroalcoholic extract of Termi...
Article
"Triphala", the Ayurvedic wonder is used traditionally for the treatment of different types of diseases since antiquity. The hydroalcoholic extracts of the three components of Triphala powder demonstrated varying degrees of strain specific antibacterial activity against multidrug-resistant uropathogenic bacteria. Terminalia chebula fruit extract wa...
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To compare the magnitude of tumour necrosis factor alpha (TNF-α) and nitric oxide (NO) response in different categories of active tuberculosis (TB) patients by ex vivo experiment. New, relapsed (recurrent), miliary and pleural effusion TB cases were recruited with matched healthy controls. TNF-α and NO were measured from the culture supernatant of...
Article
Background: AmpC beta-lactamases are cephalosporinases that confer resistance to a wide variety of beta-lactam antibiotics. Detecting AmpC-mediated resistance in gram negative organisms is a challenge for laboratories due to misleading results in phenotypic tests. There is always a search for newer and reliable methods which will be more user-frien...
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Objective To evaluate the applicability of nalidixic acid (Nx) resistance in determining reduced susceptibility to ciprofloxacin (Cp) among Salmonella enterica serovar Typhi (S. Typhi) clinical isolates.Methods The correlation between Cp MIC (minimum inhibitory concentration) values and that Nx, and between Cp MICs and zone diameter of inhibition (...
Article
Objective: Terminalia chebula Retz. (combretaceae) is called the " King of Medicine" in Tibet and is always listed at the top of the list of " Ayurvedic Materia Medica" because of its extraordinary power of healing. The present study was carried out to evaluate the possible in vitro antibacterial potential of different solvent extracts of T. chebul...
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Conventional and molecular techniques were applied to detect and characterize drug resistance of mycobacteria in the sputum samples of clinically confirmed tuberculosis. The sensitivities of mycobacterium detection by ZN staining, culture, multiplex PCR, and restriction fragment length polymorphism (RFLP) were 27.7%, 19.9%, 92.9%, and 95.7%, respec...
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The present study was carried out to evaluate the possible in vitro antibacterial potential of extracts of Eugenia jambolana seeds against multidrug-resistant human bacterial pathogens. Agar well diffusion and microbroth dilution assay methods were used for antibacterial susceptibility testing. Kill-kinetics study was done to know the rate and exte...
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To evaluate the antibacterial activity of Ocimum sanctum (O. sanctum) leaf extract, alone, and in combination with chloramphenicol (C) and trimethoprim (Tm) against Salmonella enterica serovar Typhi (S. typhi). The antibacterial activity of ethanolic extract of tulsi, O. sanctum, leaf (TLE; 500 μg) for 23 S. typhi isolates was determined following...
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Objective To develop an approach for making a selective and differential media specific for Lactobacillus rhamnosus Goldin and Gorbach (LGG) in order to distinguish it from other bacterial flora in fecal samples.Methods The current media referred as LGGM has been prepared by using MRS media ingredients (replacing dextrose with 2% sorbitol), and inc...
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This communication states the changing patterns of Salmonella enterica serovar Typhi (S. Typhi) isolates causing enteric fever in and around Kolkata, India. Among the isolates resistance to ampicillin (A), chloramphenicol (C), cotrimoxazole (Co) and tetracycline (T) were plasmid mediated; the plasmid was unstable in S. Typhi, and the other enteric...
Article
Background: Extended spectrum beta lactamases (ESBLs) and AmpC beta-lactamases conferring resistance to expanded-spectrum cephalosporins, continue to be a major problem in health care settings. Knowledge of their occurrence is essential to guide the clinicians towards the appropriate anti-microbial treatment. The purpose of this study is to evaluat...
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Objective To determine the incidence of leptospiral jaundice and find the correlation among human leptospiral cases with or without jaundice.Methods Patients suspected for leptospirosis, attending Calcutta School of Tropical Medicine, Kolkata, India during 2002 to 2008 were evaluated as per modified Faine's criterion.ResultsOut of 404 suspected cas...
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To investigate the prevalence of leptospirosis among patients from within and outside Kolkata, India, attending the Calcutta School of Tropical Medicine, for treatment during August 2002 to August 2008. The leptospirosis cases were determined on the basis of clinical, epidemiological, and biochemical factors, and were tested for leptospiral antibod...
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To search systematically for an alternative therapy with compounds particularly from plant origin. Efficacy test of different root extracts of Withania somnifera (W. somnifera) (L) Dunal against multi drug resistant (MDR) Staphylococcus aureus (S. aureus) variants was performed following the agar well diffusion method. Evaluation of susceptibility...
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The present study is an investigation on the kinetics of dimethoate biodegradation and an estimation of residual dimethoate in bacterial culture by spectrophotometry. The methylene chloride extract of the culture medium was used for determination of dimethoate through its reaction with 1 chloro-2, 4 dinitrobenzene to produce methylamine whose absor...
Article
Among the synthesized compounds, compounds (IXa), (IXc)—(IXe), (XIc), (XVa) and (XVd) show significant antitubercular activity when compared to isoniazid.
Article
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To explore the in vitro antibacterial activity of ethanolic extracts of cinnamon (Cinnamomum zeylanicum; CIN), clove (Syzygium aromaticum, CLV) and cumin (Cuminum cyminum, CMN) against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA), from Kolkata, India. The CIN, CLV and CMN were tested for their antibacterial activity again...
Article
Three new series of 4-hydroxy-8-trifluoromethyl-quinoline derivatives were synthesized through multi step reactions. All the newly synthesized compounds were characterized by spectral and elemental analyses. The structure of 5j was evidenced by X-ray crystallographic study. The newly synthesized title compounds were evaluated for their antimicrobia...
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Cholera, caused by the infection of toxigenic Vibrio cholerae (V. cholerae) to humans, is a life threatening diarrheal disease with epidemic and pandemic potential. The V. cholerae, both O1 and O139 serogroups, produce a potent enterotoxin (cholera toxin) responsible for the lethal symptoms of the disease. The O1 serogroup has two biotypes (phenoty...
Article
Three new series of quinoline-4-yl-1,2,3-triazoles carrying amides, sulphonamides and amidopiperazines were synthesized through multi-step reactions. The required intermediate, [1-(6-methoxy-2-methylquinolin-4-yl)-1H-1,2,3-triazol-4-yl]methanol (2) was prepared by treating 4-azido-6-methoxy-2-methylquinoline (1) with propargyl alcohol. Three differ...
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Introduction and objectives: Typhoid is endemic in India; multidrug resistance of Salmonella enterica serovar Typhi has been reported from Kolkata, India, but nalidixic acid (Nx) resistance has not been documented in this part of the world. The aim of this study was to determine the Nx susceptibility for S. enterica serovar Typhi isolates (1991-200...
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Introduction and objectives: Previous studies have shown the potential antibacterial activity of black tea, Camellia sinensis, extract against many pathogenic bacteria including Gram-positives (Listeria monocytogenes and Staphylococcus aureus) and Gram-negatives (Vibrio cholerae and Salmonella enterica serovar Typhi). The aim of the current study w...
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Objective: To ascertain the potential antibacterial activity of honey against clinical isolates of Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Salmonella enterica serovar Typhi (S. enterica serovar Typhi) by in. vitro methods. Methods: The partial inhibitory concentration (PIC), minimum inhibitory concentration (MIC) and...
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Objective: To evaluate the antibacterial activity of Emblica officinalis Gamin (E. officinalis; Family: Euphorbiaceae) seed and Nymphae odorata Aiton (N. odorata; Family: Nymphaeaceae) stamen extracts, alone and in combination, and in combination with amoxicillin (Ax) against Staphylococcus aureus (S. aureus). Methods: Antibacterial activity of eth...
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Objective To determine the antibiotic resistance of Vibrio cholerae (V. cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata, and to explore the role of plasmid in mediating antibiotic resistance.Methods Antibiotic susceptibility and minimum inhibitory concentration (MIC) values of antibiotics fo...
Article
Four new series of quinoline derivatives were synthesized starting from 2-trifluoromethyl aniline through multi-step reactions. In the reaction sequence, substituted aniline was cyclized to 4-hydroxy quinoline 1, which was then transformed to 4-chloro-2,8-bis(trifluoromethyl)quinoline 2. The key scaffold 4-hydrazinyl-2,8-bis(trifluoromethyl)quinoli...
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Streptococcus pyogenes is an important cause of serious bacterial infection in both adults and school-going children. On the present scenario of emerging changes observed in drug resistant pattern of S. pyogenes, this study was undertaken, from October2008 to July2009, to evaluate the trend of resistance pattern to various antibiotics by the Kirby-...
Article
A series of 26 new quinoline derivatives carrying active pharmacophores has been synthesized and evaluated for their in vitro antituberculosis activity against Mycobacterium tuberculosis H37Rv (MTB), Mycobacterium smegmatis (MC(2)), and Mycobacterium fortuitum following the broth micro dilution assay method. Compounds 13e, 13i, 13k, 14a, 14c, 14i,...
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Plantar ulcers commonly occur in leprosy patients, which usually recur and cause morbidity in such cases. The aim of the study is to find out the bacteriological profile of these ulcers and to find out the antibiotic susceptibility of the isolates so that appropriate drugs may be chosen for treatment and for prevention of recurrence. Fifty-six samp...
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In April 2007, a slum of South Dumdum municipality, West Bengal reported an increase in fever cases. We investigated to identify the agent, the source and to propose recommendations. We defined a suspected case of typhoid fever as occurrence of fever for > or = one week among residents of ward 1 of South Dumdum during February - May 2007. We search...
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The emergence of Salmonella enterica serovar Typhi isolates resistant to ciprofloxacin and 3rd-generation cephalosporins is a concern for physicians in developing countries. This study assessed the in vitro activity of gentamicin and amikacin against 464 S. entenca serovar Typhi isolates obtained from blood of patients clinically suspected of enter...
Article
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In this communication, the ciprofloxacin-trimethoprim (Cp-Tm) combination showed synergistic (Fractional Inhibitory Concentration, FIC index 0.399) and additive (FIC index 0.665-0.83) effects against Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates having Cp MICs 10 microg/ml and Cp 0.66 microg/ml, respectively, following agar dilution che...
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The aim of this study was to evaluate the possible antibacterial potential of Chebulic myrobalan extracts (cold aqueous, hot aqueous and ethanol) against multi-drug resistant bacterial pathogens. For this purpose both clinical isolates (methicillin resistant Staphylococcus aureus and trimethoprim-sulphamethoxazole resistant uropathogenic Escherichi...
Article
Mycobacterium tuberculosis is a facultative intracellular pathogen that flourishes inside the host macrophages. This organism has the ability to deactivate the cell-mediated immune responses involving the down-regulation of pro-inflammatory cytokines, T cell proliferation, apoptosis of CD4+T cells and impairment of the expression of MHC Class II mo...
Article
Mycobacterium tuberculosis exerts its pathogenic effects mainly via its cell wall glycolipid called Mannosylated Lipoarabinomannan (Man-LAM), which subverts the cellular inflammatory responses by the suppression of superoxide anion generation in earlier hours, and nitric oxide (NO) generation at later hours of pathogenic invasion. In this paper, we...
Article
Dimethoate-degrading enzymatic activity in Bacillus licheniformis, Pseudomonas aeruginosa, Aeromonas hydrophila, Proteus mirabilis and Bacillus pumilus was found to be 6.4, 1.760, 4.09, 1.196 and 0.505 units/mg protein, respectively. The Escherichia coli C600 transconjugants of the isolated bacterial strains also exhibited dimethoate-degrading enzy...
Article
Microbial degradation of pesticide has been recognized as a potential solution for the disposal of pesticide. Two bacterial strains namely Bacillus licheniformis and Pseudomonas aeruginosa, which were isolated from water and fish intestine, respectively, were allowed to grow in mineral salt solution. The acetonitrile extracts of the bacterial cultu...
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The present communication states the effect of UV irradiation on antibiotic resistance of Salmonella enterica serovar Typhi strain B18 from blood culture, and evaluates the role of R-plasmid in mediating UV-resistance. The colonies of the multidrug resistant (MDR) S. Typhi strain B18 were treated with UV light (260 nm) to check any changes of its a...
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The aim of this study was to document the changing clinical profile and prognosis of acute diarrhea in infants. This was a prospective observational study with follow-up. Demographic, anthropometric, and clinical data were collected in children younger than 1 year with acute diarrhea. Stool was examined under the microscope, cultured, tested for pr...