Nilambari Gurav

• Doctor of Pharmacy
• Professor (Assistant) at PES’s Rajaram and Tarabai Bandekar College of Pharmacy, Ponda, Goa University, Goa

The present study validates the biomimetic synthesis of silver nanoparticles using an endophytic fungus, Colletotrichum siamense Prihast (Colleto-AgNPs) derived from Annona muricata leaves. The study also investigates the impact of various process variables, including reductant concentration, the ratio of AgNO3 to reductant concentration, reaction...

Traditional medicines have reportedly treated SARS-CoV-2 infection. Substantial evidence shows that fish oil supplements promote human immune function, suggesting they may lessen susceptibility to SARS-CoV-2 infection and suppress viral replication by inducing interferon. Fish oil was subjected to partition chromatography and separated into two com...

Abstract Background The emergence and evolution of SARS-CoV-2 resulted a severe threat to public health globally. Due to the lack of an effective vaccine with durable immunity, the disease transited into the endemic phase, necessitating potent antiviral therapy including a scientific basis for current traditional herbal medicine. Objective This st...

Studies have reported the potential role of Boswellic acids (BAs), bioactive pentacyclic triterpenes from Boswellia serrata, in treating rheumatoid arthritis (RA). However, poor water solubility and limited oral absorption are restricting factors for its better therapeutic efficacy. Based on these assumptions, the current study set out to develop n...

Fungi-mediated biosynthesis of silver nanoparticles is one of the promising nanomanufacturing processes. Using endophytic fungi isolated from potential medicinal plants has emerged as a novel approach for producing several metal oxide nanoparticles in a green and cost-effective manner. The present study validates the biomimetic synthesis of silver...

The present invention provides Curcuma longa callus enriched in secondary metabolites obtained from a lateral bud of C. longa by a process comprising growing C. longa lateral bud, inducing callus cell culture from C. longa lateral bud in a Murashige-Skoog medium comprising at least one phyto-hormone and sub-culturing said callus cell culture to obt...

Despite the tremendous potential of phytomedicines (plant-derived drugs), their chemical and biological barriers and conventional/traditional dosage forms constrain the desired therapeutic efficacy with reduced side effects. The high molecular weight/size, poor aqueous/lipid solubility, and decreased plasma membrane permeability of these nutritiona...

All over the world, cancer is the leading cause of death. On the other hand, the efficacy of existing mainstream cancer treatments is unsatisfactory for a range of tumors. To begin with, most cancer treatments are non-specific, meaning they impact both cancer cells and their non-cancerous counterparts. Furthermore, due to challenges in administrati...

The current work is concerned with the fabrication of titanium dioxide (TiO2) nanoparticles (NPs) employing Capparis zeylanica leaf extract, as well as the investigation of the antidiabetic potential of the synthesized nanoparticles in relation to food-borne infections. Various spectroscopic and microscopic approaches were used to characterize the...

The book entitled “Antidiabetic Drug Discovery from Natural Products” provides up-to-date and in-depth information, practical understanding, and guidance, but in a comprehensive, systematic, and easy-to-understand manner to scientists, researchers, students, and teachers on critical and emerging topics biochemical and pharmacology topics (enumerate...

The promising therapeutic implications of nanoparticles have spurred their development for biomedical applications. An eco-friendly methodology synthesizes gold nanoparticles using Cordyceps militaris, an edible mushroom (Cord-Au-NPs), using a quality-by-design approach (central composite design). UV-visible spectroscopy analysis revealed an absorp...

The present research demonstrates the formation of zinc oxide nanoparticles facilitated by Cissus quadrangularis (CQ-ZnONPs) and subsequent synthesis of chitosan-conjugated nanocomposites (CQ-CS/ZnONCs) along with their biological assessment. The biosynthesized nanoparticles and nanocomposites were physicochemically characterized and therapeuticall...

The central nervous system (CNS) is an essential organ system in our body responsible for coordinating and controlling all of the activities performed by the various body parts. As a result, medicine and the therapy used to treat conditions related to the CNS play an important part in clinical practice. However, the conventional clinical treatment...

The present invention provides a biological method for the preparation of a compound of formula I; said method comprising the following steps: inoculating the Streptomyces violaceorubidus strain into at least one nutrient medium comprising at least one carbohydrate source to obtain an inoculum, fermenting the, inoculum at a. pH ranging-between .5.5...

The present research work aimed at synthesizing chitosan-coated Zinc oxide nanocomposites (NS-CS/ZnONCs) by a bio-inspired method using an aqueous extract of Nigella sativa (NS) seeds and employing a quality-by-design approach (Box-Behnken design). The biosynthesized NS-CS/ZnONCs were physicochemically characterized and subjected to their in-vitro...

Biogenic and biomimetic nanosystems (BBS) are the advanced hybrid nanomaterial used for treatment and diagnosis. These nanosystems' true bench-to-bedside potential makes them a more promising approach for biomedical applications. These nanoparticles are synthesized using biological microorganisms such as bacteria, algae, fungi, etc. A biosynthetic...

Ethnopharmacological relevance: Processing cow ghee (clarified butterfat) with therapeutic herbs, i.e. ghrita, is recognized for augmenting the therapeutic efficacy of plant materials. Ashwagandha ghrita (AG) is an effective Ayurvedic formulation consisting of Indian ginseng, i.e., Withania somnifera (L.) Dunal is the main constituent used to treat...

The present study intends to formulate, characterize and appraise the phospholipid-based nanovesicular system for enhanced delivery of Hesperetin (HT). The quality by design (QbD) approach was employed to prepare Hesperetin naturosomes (HTN) using the solvent evaporation technique and assessed for physicochemical and pharmacological attributes. The...

Toxicodendron succedaneum (L.) Kuntze (Anacardiaceae) is a deciduous tree widely distributed in South and Southeast Asia. The resin of T. succedaneum is used in decorating traditional handicrafts, and resinous latex is poisonous. The plant yields a commercially important wax and treats asthma, cough, fever, ear infections, pulmonary infections, dia...

The present invention provides a combustible coil or stick formulation for controlling/repelling/killing mosquitoes. The coil or stick formulation of the present invention essentially contains Neem, Vekhand, Tulsi, Ajowan, Coconut shell powder, Saw dust, Cow ghee, Peppermint oil, Lemongrass oil, Cow dung, Starch paste and Maida. The present inventi...

Annona muricata L. (Annonaceae) is widely distributed throughout tropical and subtropical countries and is also known as the 'soursop' plant due to edible fruits' sour and sweet taste. Various parts of A. muricata have been used as traditional medicine in treating multiple diseases. The present investigation deals with the study of pharmacognostic...

Chronic, non-healable wounds have been a threat throughout history and have consumed centuries of traditional and modern research. In wound repair, a growing variety of novel treatments have been developed. At various stages of wound healing, nanostructure systems are employed. The drug may be synthesized at the nanoscale to act as a “provider,” or...

The present research demonstrates the Cordyceps militaris (a medicinal mushroom) mediated fabrication of zinc oxide nanoparticles (Cor-ZnONPs) and its therapeutic investigation. The biosynthesized Cor-ZnONPs were characterized by UV–Visible spectroscopy, FT-IR, XRD, SEM with EDX, HRTEM, EDX, particle size, and zeta potential analysis and evaluated...

Hesperetin (HT) is a polyphenolic compound with anticarcinogenic, tumor necrosis, and antioxidant properties. The present study reports the fabrication, optimization, and evaluation of HT-loaded nanosponges (HTN)-based gel (HTNG) for sustained anti-inflammatory effect. HTN formulation was prepared by quasi emulsion solvent diffusion method using a...

Bhallatakadi Ghrita' (BG), comprising the plant extracts of Semecarpus anacardium L., Argemone mexicana L., Cocculus hirsutus L., and Woodfordia fruticosa K. 'Murcchana samskara' of ghee before any 'ghrita-paka' preparation evidenced the maximum acceptability for topical application. The current study dealt with the effect of the 'Murcchana' proces...

The present research deals with the rutin mediated synthesis of titanium dioxide nanoparticles (TiO2NPs), their characterization, evaluation of antibacterial, antidiabetic, and antioxidant properties. XRD spectrum of uncalcined TiO2NPs did not show any distinct diffraction peaks revealing its amorphous nature, while calcinated TiO2NPs showed promin...

Cassia glauca is reported as anti-diabetic medicinal plant and is also used as an ethnomedicine. However, its mode of action as an anti-diabetic agent has not been clearly elucidated. Hence, the present study investigated the probable mechanism of action of C. glauca to manage diabetes mellitus via network pharmacology and molecular docking and sim...

The present invention provides a method for producing Boerhaavia diffusa callus. The method consisting of providing explants of B. diffusa; growing the selected explants of B. diffusa under sterile conditions; generating/inducing callus cell cultures of B. diffusa from the selected explants of B. diffusa in a medium; and sub-culturing said callus c...

Objective: A randomized, open-label, balanced, two-treatment, two-period, two-sequence, single-dose, crossover bioequivalence study comparing Rizatriptan 10 mg Orally Disintegrating Strips (ODS, test) with that of established Oral Lyophilisate Rizatriptan 10 mg, Maxalt-MLT® (reference) was conducted in 24 healthy male volunteers under fasting condi...

Saikosaponins are major biologically active triterpenoids, usually as glucosides, isolated from Traditional Chinese Medicines (TCM) such as Bupleurum spp., Heteromorpha spp., and Scrophularia scorodonia with their antiviral and immunomodulatory potential. This investigation presents molecular docking, molecular dynamics simulation, and free energy...

Background Since December 2019, SARS-CoV-2 had been a significant threat globally, which has accounted for about two million deaths. Several types of research are undergoing and have reported the significant role of repurposing existing drugs and natural lead in the treatment of COVID-19. The plant Phyllanthus emblica (Synonym-Emblica officinalis)...

Traditionally, Withania somnifera is widely used as an immune booster, anti-viral, and for multiple medicinal purposes. The present study investigated the withanolides as an immune booster and anti-viral agents against the coronavirus-19. Withanolides from Withania somnifera were retrieved from the open-source database, their targets were predicted...

Bioflavonoids are the largest group of plant-derived polyphenolic compounds with diverse biological potential and have also been proven efficacious in the treatment of Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). The present investigation validates molecular docking, simulation, and MM-PBSA studies of fiftee...

Background: Ghee is widely considered as the Indian name for clarified butterfat and processing of ghee with therapeutic herbs i.e. ghrita is renowned for augmenting their medicinal properties. Kaamdev ghrita (also known as 'VajikaranaRasayana') is cow ghee based classical Ayurvedic formulation from the aphrodisiac category, which is used to ameli...

COVID-19 has ravaged the world and is the greatest of pandemics in human history, in the absence of treatment or vaccine the mortality and morbidity rates are very high. The present investigation was undertaken to screen and identify the potent leads from the Indian Ayurvedic herb, Asparagus racemosus (Willd.) against SARS-CoV-2 using molecular doc...

COVID-19 has ravaged the world and is the greatest of pandemics in modern human history, in the absence of treatment or vaccine, the mortality and morbidity rates are very high. The present investigation identifies potential leads from the plant Withania somnifera (Indian ginseng), a well-known antiviral, immunomodulatory, anti-inflammatory and a p...

The Public Health Emergency of International Concern declared the widespread outbreak of SARS-CoV-2 as a global pandemic emergency, which has resulted in 1,773,086 confirmed cases including 111,652 human deaths, as on 13 April 2020, as reported to World Health Organization. As of now, there are no vaccines or antiviral drugs declared to be official...

The effects of explant source, medium composition, and growth regulators were examined in callus lines of Curcuma longa. Callus cultures were induced from leaf sheath, leaf lamina, leaf base, apical buds, and lateral buds. The growth potential of established callus lines varied with a combination of phytohormones and culture medium. The greatest ca...

Background Withania somnifera (L.) (family-Solanaceae), known as ‘Indian ginseng’ or ‘Ashwagandha’ is acclaimed as an effective adaptogen, immunomodulator, aphrodisiac and sedative. Ashwagandha ghrita is a recognized ghee based Ayurvedic formulation. Few ancient texts suggest murcchana process for preparation of Ashwagandha ghrita. Objective The s...

Objectives: To develop, characterize and evaluate the phospholipid based nanovesicular system for enhanced delivery of Hesperetin (HT). Methods: Naturosomes of Hesperetin (HTN) with phospholipid were prepared by solvent evaporation technique using Design of Experiment (DOE) approach to optimize the formulation and process variables. The prepared na...

Background: Withania somnifera (L.) (family-Solanaceae), known as ‘Indian ginseng’ or ‘Ashwagandha’ is acclaimed as an effective adaptogen, immunomodulator, aphrodisiac, sedative etc. ‘Ashwagandha ghrita’ is a recognized ghee based Ayurvedic formulation. In some ancient texts, murchan process is suggested for preparation of Ashwagandha ghrita. Obje...

The present invention provides a method for producing Curcuma longa callus. The method consisting of providing explants of C. longa; growing the selected explants of C. longa under sterile conditions; generating/inducing callus cell cultures of C. longa from the selected explants of C. longa in a medium; and sub-culturing said callus cell cultures...

Thorough information of the structure of plant cells and tissues is the basic requirement for actual interpretation of morphology, physiology, and phylogeny. Furthermore, proper sectioning of the crude drug (plant material) is the initial step to proceed with the histological study of the same. The present chapter gives a brief idea about the neces...

Adulteration and misidentification of herbal drugs can cause serious health problems to consumers. The first step in quality control of medicinal plants is ensuring the authenticity of the desired species for intended use, with anatomical study playing a critical role in identifying and authenticating medicinal plants. A product of numerous years o...

Bergenia ligulata Wall is considered amongst the high valued medicinal herbs and one of the important examples of controversial drugs which is popularly known as 'Paashanbheda' (meaning 'to dissolve the stone') in Indian systems of medicine. The phytochemical studies have shown the presence of many secondary metabolites belonging to coumarins, flav...