Nikolay D Solovyev

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• PhD
• Lecturer at Institute of Technology Sligo

Se speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma–dynamic reaction cell–mass spectrometry (ICP-DRC-MS), was employed. Species identification was done by standard matched retention time, sta...

Exposure to selenium, and particularly to its inorganic forms, has been hypothesized as a risk factor for amyotrophic lateral sclerosis (ALS), a fast progressing motor neuron disease with poorly understood etiology. However, no information is known about levels of inorganic and some organic selenium species in the central nervous system of ALS pati...

A new method of simultaneous redox speciation of iron (II/III), manganese (II/III), and copper (I/II) in cerebrospinal fluid (CSF) has been designed. For the separation of redox species strong cation exchange chromatography (SCX) with isocratic elution was employed. Species were detected using inductively coupled plasma sector field mass spectromet...

The essential trace element, selenium (Se), is crucial to the brain but it may be potentially neurotoxic, depending on dosage and speciation; Se has been discussed for decades in relation to Alzheimer’s disease (AD). Selenoprotein P (SELENOP) is a secreted heparin-binding glycoprotein which serves as the main Se transport protein in mammals. In viv...

Biomedical analytical methods often rely on indirect measurements, such as immunoassays, which can lack effective metrological traceability. In the nephelometric determination of ceruloplasmin (Cp), an important protein whose circulating level is altered in Wilson's disease (WD), the anti-Cp antibody used is not specific for the biologically active...

Amyotrophic lateral sclerosis (ALS) is a lethal progressive neurodegenerative disease, characterized by a loss of function of upper and lower motor neurons. This study aimed to explore probable pathological alterations occurring in individuals with ALS compared to neurologically healthy controls through the analysis of cerebrospinal fluid (CSF), a...

Advances in inductively coupled plasma mass spectrometry and the methods used to prepare isotopically enriched standards, allow for the high accuracy measurement of metalloproteins by isotope dilution mass spectrometry. This technique has now reached a level of maturity whereby a step change in the accuracy, precision, and traceability of, in parti...

Glow discharge (GD) source gained an increased level of attention in relation to the analysis of volatile organic compounds (VOCs) since past work showed that this soft ionization method allowed direct analysis of VOCs with minimal fragmentation, however, the issue of fragmentation was not previously studied in detail. The aim of the present work w...

BACKGROUND A standard approach to study the anticancer activity of novel drugs is their testing in animals with inoculated tumors, which has some limitations. An alternative is the use of spontaneous or carcinogen-induced tumor models as they have better translation potential. The carcinogen-induced and transgenic tumor models were used to assess t...

Ethylene glycol (EG) is widely used in various economic and industrial fields. The demand for its efficient separation and recovery from water is constantly growing. To improve the pervaporation characteristics of a poly(2,6-dimethyl-1,4-phenylene oxide) (PPO) membrane in dehydration of ethylene glycol, the modification with graphene oxide (GO) nan...

A broadband collision-induced dissociation (bbCID) fragmentation mode was proposed for liquid chromatography–mass spectrometric targeted analysis of tryptic peptides obtained from proteins in samples of decoration paint coating. In this approach, a mass spectrometric dataset contains the information on the parent and all fragment ions. This maintai...

Platinum (Pt) antineoplastic agents remain indispensable for the treatment of oncological disease. Pt-based drugs are mainly used in the therapy of ovarian cancer and non-small-cell lung carcinoma. A novel platinum-containing antineoplastic agent BP-C1 is a complex of diamminoplatinum with an oxygen-donor polymeric ligand of benzene-polycarboxylic...

A recent trend in glow discharge analysis, previously considered as a ‘purely inorganic’ technique, is related to the effective ionisation of volatile organic compounds (VOCs). This approach was demonstrated to be capable of analysing VOCs in both model gas mixtures and ambient air. In the current study, the possibility of the direct determination...

A recent trend in glow discharge analysis, previously considered as a ‘purely inorganic’ technique, is related to the effective ionisation of volatile organic compounds (VOCs). This approach was demonstrated to be capable of analysing VOCs in both model gas mixtures and ambient air. In the current study, the possibility of the direct determination...

Transport characteristics of sodium alginate (SA) membranes cross-linked with CaCl2 and modified with fullerenol and fullerene derivative with L-arginine for pervaporation dehydration were improved applying various approaches, including the selection of a porous substrate for the creation of a thin selective SA-based layer, and the deposition of na...

Selenium (Se) is known to contribute to several vital physiological functions in mammals: antioxidant defense, fertility, thyroid hormone metabolism, and immune response. Growing evidence indicates the crucial role of Se and Se-containing selenoproteins in the brain and brain function. As for the other essential trace elements, dietary Se needs to...

Novel dense and supported (polyacrylonitrile substrate) mixed matrix membranes based on biopolymer sodium alginate (SA), modified by Zr-MOFs were developed to improve pervaporation dehydration properties of a parent SA membrane. The following Zr-MOFs were synthesized and tested as modifiers: unmodified UiO-66 and modified UiO-66(NH2)-AcOH and UiO-6...

Alzheimer’s disease (AD) is characterized by accumulation of tau and amyloid-beta in the brain, and recent evidence suggests a correlation between associated protein aggregates and trace elements, such as copper, iron and zinc. In AD, distorted brain redox homeostasis and complexation by amyloid-beta and hyperphosphorylated tau may alter the isotop...

Recently glow discharge (GD) mass spectrometry, which is conventionally used to analyze solid samples, was successfully applied for ionization and the subsequent determination of volatile organic compounds (VOCs). In the present study, the possibility of direct determination of VOCs in ambient air using time-of-flight mass spectrometry with microse...

Hyperthermic intraperitoneal chemoperfusion (HIPEC) is an established form of locoregional chemotherapy of peritoneum tumors. However, its efficacy and safety status remain a controversy, partially, due to scarce data on pharmacokinetics and toxicity profile of drugs under HIPEC. In the current study, 24 female Wistar rats were randomly assigned to...

The determination of nonmetals, first of all, the most electronegative ones—nitrogen, oxygen, fluorine, chlorine, and bromine, poses the highest challenge for element analysis. These elements are characterized by high reactivity, volatility, high ionization energy, and the absence of intensive spectral lines in the optical spectral range. Conventio...

Combined hollow cathode microsecond direct current pulsed glow discharge time-of-flight mass spectrometry (CHC µs-DС-PGD TOFMS) system has proved its efficiency for the quantification; however, it was not properly tested for the purposes of depth analysis up-to-now, to the best of the authors' knowledge. The aim of the current study was to investig...

Direct analysis of matrix and admixture elements in non-conducting crystals is a relevant analytical task in terms of quality assurance of optical materials. The current study aimed to develop a method capable to assess the inhomogeneity of optical crystals with sufficient sensitivity. K1−xRbxTiOPO4 (x = 0.002 and 0.05) and KGd1−yNdy(WO4)2 (y = 0.0...

Amyotrophic lateral sclerosis is a progressive neurodegenerative disease characterized by a loss of function of motor neurons. The etiology of this disorder is still largely unknown. Gene-environment interaction arises as a possible key factor in the development of amyotrophic lateral sclerosis. We assessed the levels of trace metals, copper (Cu),...

Alzheimer’s disease (AD) is the most common neurodegenerative disease associated with cognitive decline, loss of memory, and progressive cerebral atrophy. The trace element selenium (Se) is known to be involved in brain pathology. Selenoprotein P (SELENOP), as the main Se transport protein, is, to a great extent, responsible for maintaining Se home...

Rationale: Dopants in ionic conductors play a crucial role in achieving the required electrochemical properties. A slight variation in their concentration considerably affects crystals' conductivity and their applicability as ionic conductors and laser materials. To ensure the growth of high-quality fluoride crystals, adequate approaches for the q...

History of the development of a hollow cathode as a version of a discharge cell for optical emission spectroscopy and glow-discharge mass spectrometry is discussed. Special attention was paid to the contribution of colleagues from the Department of Analytical Chemistry at the St. Petersburg State University to research, development, and implication...

Diabetes mellitus is a chronic metabolic disease caused by insulin deficiency (type I) or dysfunction (type II). Diabetes is a threatening public health concern. It is considered as one of the priority non-communicable diseases, due to its high and increasing incidence, the associated healthcare costs, and threatening medical complications. Two tra...

Cu isotope fractionation was investigated in the human neuroblastoma SH-SY5Y cell line, in a proliferating/tumor phase (undifferentiated cells), and in a differentiated state (neuron-like cells), induced using retinoic acid (RA). The SH-SY5Y cell line displays genetic aberrations due to its cancerous origin, but differentiation drives the cell line...

A method has been developed for the determination of 24 elements (As, B, Ce, Co, Dy, Fe, K, La, Lu, Mg, Mn, Na, Nb, Nd, P, Pr, Rb, S, Sb, Si, Sm, Th, Ti, and U) in ore samples by pulsed direct current glow discharge time-of-flight mass spectrometry (PGD-TOF-MS). Sample treatment consisted of pressing the powdered samples into 10 mm diameter aluminu...

There is much contradiction between different experimental studies on beryllium (Be) toxicity. The majority of studies focus on occupational pathologies, caused by the exposure to Be dust. However, Be pollution may affect wide population groups through other exposure routes. The discrepancies between experimental studies may be attributed to the la...

Neon glow discharge was tested for the direct analysis of solid dielectric materials by pulsed glow discharge time-of-flight mass spectrometry with an emphasis on the elements with high ionisation energy. The findings for neon were compared to conventional argon glow discharge. Fluorine doped potassium titanyl phosphate (KTP) single crystals were u...

Organophosphates’ toxic effect causes covalent binding to serine-198 in the active site of human plasma butyrylcholinesterase (BChE) with loss of enzymatic function (covalent inhibition). Mass spectrometric detection of modified FGESAGAAS peptide at the active site is a powerful exposure biomarker tool. The aim of this study was to develop mass spe...

In the present study, novel pervaporation (nonporous, dense) and ultrafiltration (porous) membranes based on polyphenylene isophthalamide (PA) modified by Pluronic F127 (up to 20 wt%) used as a modifier and a pore-forming agent were developed and investigated. The structural parameters of the membranes obtained were studied by IR-spectroscopy, nucl...

Although aluminum chronic neurotoxicity is well documented, there are no well-established experimental protocols of Al exposure. In the current study, toxic effects of sub-chronic Al exposure have been evaluated in outbreed male rats (gastrointestinal administration). Forty animals were used: 10 were administered with AlCl3 water solution (2 mg/kg...

Intensive rice-wheat cultivation cycle in Northern belt of India in general and in the State of Punjab in particular results in large volumes of straw and other post-harvest residue annually. The agricultural area, bordering the districts of Nawanshahr and Hoshiarpur, is popularly known as the seleniferous belt of India. The agri-residues, generate...

Sapphire (α-Al2O3, transparent corundum) single crystals were analyzed with pulsed direct current glow discharge mass spectrometry. Combined hollow cathode was used as a discharge cell. To obtain stable sputtering of dielectric material, a formation of initial surface conductivity via preliminary vacuum deposition of thin metallic layer was propose...

Early detection of lung cancer usually markedly increases the efficiency of therapy. However, the currently employed diagnostic approaches are not sufficiently effective, resulting in late detection of the disease and high patient mortality. Therefore, development of a high-throughput and reliable diagnostic method is a priority task requiring fast...

A pulsed direct current glow discharge time-of-flight mass spectrometry (GD TOF MS) method for the quantification of fluorine in insoluble crystal materials with fluorine doped potassium titanyl phosphate (KTP) KTiOPO4:KF as an example has been proposed. The following parameters were optimized: repelling pulse delay, discharge duration, discharge v...

Direct quantification of oxygen in dielectric materials using non-destructive or nearly non-destructive techniques still remains a nontrivial task. Simultaneous assessment of oxygen with other elements in a single analytical procedure is even more challenging. In the current study, a method of direct determination of oxygen and other matrix element...

Introduction: Molybdenum (Mo) is an essential element for mammalian organisms. Three important enzyme families contain molybdenum-based cofactors. At the same time, metabolism of this element has attracted relatively low attention up-to-now. Even the transport of this element in the body was studied scarcely. Aim: Investigate molybdenum speciation...

Background: Although an increasing role of genetic susceptibility has been recognized, the role of environmental risk factors in amyotrophic lateral sclerosis (ALS) etiology is largely uncertain; among neurotoxic chemicals, epidemiological and biological plausibility has been provided for pesticides, the heavy metal lead, the metalloid selenium, a...

Element speciation offers deeper insight into the molecular mechanisms of disease by determining element species pattern. Thus, having great potential for investigating neurodegeneration in Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and mild cognitive impairment, speciation is increasingly considered in epidemiological...

Up-to-now the highest degree of attention was paid to the pathology of respiratory tract disorders, associated with human exposure to beryllium (Be). However, berylliosis as the most common pathology may be associated mostly with particles dispersity, whereas toxicity of Be2+ ions itself is much less studied. The difficulties in studying of the lat...

Analytical challenges of selenium (Se) species determination, using hyphenated techniques, are discussed. Se is ‘complicated’ element for the inductively coupled plasma (ICP) based techniques, owing to relatively low sensitivity and abundant interferences. Since emission lines of Se are insensitive, nearly exclusive mass spectrometry (MS) detection...

the Chernobyl ‘lava’ and hot particles were collected. Isotope analysis of Chernobyl specimens is the subject of special interest, since unexpected results on 238U/235U ratio has been reported previously. Although, over 30 years have passed since the Chernobyl accident, these samples are still a source of important information about the catastrophe...

After the Chernobyl nuclear accident in 1986, numerous specimens of the so-called ‘fuel-containing masses’ or the Chernobyl ‘lava’ and hot particles were collected. Isotope analysis of Chernobyl specimens is the subject of special interest, since unexpected results on ²³⁸U/²³⁵U ratio has been reported previously. Although, over 30 years have passed...

Platinum-containing neoplastic agents with physiologically active ligands seem to be a promising direction in anticancer drug design. CDBPA (BP-C1) is a novel promising antineoplastic agent, containing polymer ligand of natural origin (international patent WO2013/143549 A1). Polymer ligand of CDBPA has a highly functionalised polyphenolic backbone,...

FIELD: medicine. SUBSTANCE: invention relates to experimental toxicology, and can be used to study the mechanisms of the toxic effect of beryllium soluble forms. To do this, simulation of intoxication with beryllium salts is carried out by single or prolonged repeated intraperitoneal injection of beryllium salts. Salts are introduced as a solution...

Radiotherapy is widely spread approach for the treatment of certain malignancies. However, this is rather hazardous and invasive techniques; thus, the design and development of novel radiomitigators is of great importance for the restorative therapy of oncological patients. BP-C2 is a complex of molybdenum (Mo) with lignin-derived benzene-poly-carb...

In the current study, a biomonitoring of 18 hair trace elements (Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, V, Zn, Ca, Na and P) in school children from Leningradskaya Oblast’ is reported. A case group, residing in a proximity to the toxic waste disposal grounds (Krasniy Bor), has been assessed vs. controls from a non-urban settlement Selt...

The performance of acid extraction, dry ashing, and microwave digestion was evaluated for voltammetric cadmium quantification in oil crops with sunflower (Heliánthus ánnuus) seeds as an example. Standard additions, reference material analysis, and comparison to graphite furnace atomic absorption spectrometry were employed for validation. The main a...

Multiple biological functions of selenium manifest themselves mainly via 25 selenoproteins that have selenocysteine at their active centre. Selenium is vital for the brain and seems to participate in the pathology of disorders such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and epilepsy. Since selenium was shown to b...

A direct method of uranium and thorium determination in non-conducting geological samples using time-of-flight mass spectrometry with pulsed glow discharge was proposed. The following rock specimens were analysed: metamict zircon, metamict rinkite, metamict samarskite (Y-Fe-niobate), pyrochlore and jacinth. For sample sputtering combined hollow cat...

Cover page of the issue

A method of platinum quantification in whole blood samples after microwave digestion using sector field inductively coupled plasma mass spectrometry has been developed. The following analytical figures of merit have been established: limit of detection 1.1 µg/L for blood samples, dynamic range 3.6-200 µg/L, intra-day precision (relative standard de...

A digestion free blood arsenic assessment method based upon in situ trapping of arsenic hydride in coated graphite furnace was developed. Double layer coating of the furnace with Na2WO4 – H2PtCl6 was used. Arsenic limit of detection (3σ) for whole blood samples was 0.1 μg/L. Dynamic range was 60-1000 pg As. Relative standard deviation for blood sam...

Methods of whole blood trace element determination by Graphite furnace atomic absorption spectrometry (in the variant of Zeeman's modulation polarization spectrometry) have been proposed. They do not require preliminary sample digestion. Furnace programs, modifiers and blood dilution factors were optimized. Seronorm™ human whole blood reference mat...

Se-speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma –dynamic reaction cell- mass spectrometry was employed. Species identification was done by standard matched retention time, standard additi...

Determination of aluminum (Al), beryllium (Be), cadmium (Cd), chromium (Cr), mercury (Hg), manganese (Mn), nickel (Ni), lead (Pb), and thallium (Tl) concentrations in human blood using high-frequency modulation polarization Zeeman graphite furnace atomic absorption spectrometry (GFAAS) was performed. No sample digestion was used in the current stud...

General approaches to the determination of trace elemental species in biological media were summarized. Main attention was paid to the speciation analysis of clinical samples. Hybrid techniques including species separation and their sequential element specific detection are observed as a methodic approach of priority importance. Methodological solu...

Main approaches to the trace element analysis of biological fluids and problems appearing in this case are considered. The specific character of these approaches is illustrated by the examples of the analysis of real samples for various trace elements. The advantage of methods for the direct determination of trace elements in these samples is demon...

New technique for the determination of Mn, Cr, Ni in human whole blood based on Graphite furnace atomic absorption spectrometry with High-frequency modulation polarization Zeeman background correction was developed. During the technique development graphite furnace program was optimized. Performance of some conventional matrix modifiers was invest...

A new technique of blood thallium direct determination based on graphite furnace atomic absorption spectrometry with Zeeman background absorption correction system was designed. The developed technique does not require sample digestion. Sample treatment includes only a fivefold per volume dilution of blood sample with 0.1% (m/v) Triton X-100. L'vov...