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Nikola Milašinović

Nikola Milašinović
University of Michigan / University of Criminal Investigation and Police Studies / Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade

PhD

About

31
Publications
3,775
Reads
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455
Citations
Additional affiliations
January 2013 - present
The Academy of Criminalistic and Police Studies, Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade
Position
  • Research Assistant
October 2010 - present
Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade
Position
  • Research Associate

Publications

Publications (31)
Article
The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were c...
Article
Full-text available
The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate funct...
Article
Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems...
Article
This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan....
Conference Paper
Cyanide has been used as a poison for thousands of years. Cyanides comprise a wide range of compounds of varying degrees of chemical complexity and toxicity, all of which contain a CN moiety. Humans are exposed to cyanides from a broad range of natural and anthropogenic sources. Plant toxins, such as glycosides, can be present in the whole plant or...
Article
Incorporation of mucoadhesive polymers is one of the first efficient modification of conventional liquid ophthalmic formulations in order to prolong the residence time at ocular surface. The aim of this work was formulation of viscous ophthalmic vehicles/ocular lubricants containing derivative of guar gum, chitosan and cellulose derivatives (hyprom...
Article
Supercritical carbon dioxide (scCO2) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with sc...
Article
Statement of significance: The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisolation applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical medi...
Article
Full-text available
In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different t...
Chapter
The aim of food processing is to produce food that is palatable and tastes good, extend its shelf-life, increase the variety, and maintain the nutritional and healthcare quality of food. To achieve favorable processing conditions and for the safety of the food to be consumed, use of food grade microbial enzymes or microbes (being the natural biocat...
Article
Full-text available
The identification of latent fingerprints is crucial for the security agencies in order to identify persons suspected of having committed criminal acts. Latent prints often remain as random impressions onto the surfaces of various objects. Huge progress has been made in the identification processes using rolled and plain impressions fingerprints, w...
Article
The aim of this study was to investigate the encapsulation of α-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was...
Article
Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport pro...
Article
Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan micropartic...
Article
In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. Th...
Article
Full-text available
The application of poly(acrylamide-co-sodium methacrylate) hydrogel for the removal of Cu2+ and Cd2+ ions from aqueous solutions has been investigated as a function of pH, adsorbent dose and temperature. FTIR spectra showed that –NH2 and –COOH groups are involved in both metal ions adsorption which followed pseudo-first order kinetics. Non-linear r...
Article
Full-text available
Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the i...
Data
Tables 1-3 present the effect of temperature, pH of the buffer solution and lipase concentration on the lipase activity after entrapment.
Article
Full-text available
This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum tempera...
Article
Technical forensics is a very broad scientific field. This paper reports one of its aspects, which refer to the testing of electrical conductors following a fire in order to identify the real cause of its appearance, namely providing information whether or not the electric cord damage caused the fire and to offer contribution to the authorities. Fo...
Article
Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equili...
Article
A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was...
Article
Full-text available
To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing...
Article
Full-text available
This paper deals with the synthesis of copolymer hydrogels of N-isopropylacrylamide, itaconic acid and chitosan. During synthesis, pH of the reaction solution and temperature were varied. It was found that the reaction at higher temperature, above lower critical solution temperature (LCST) value of N-isopropylacrylamide resulted in a formation of p...
Article
Novel pH‐sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid were prepared in two steps. Chitosan was first ionically crosslinked with itaconic acid, after which a free radical polymerization and crosslinking of the chitosan/itaconic acid network was performed by adding methacrylic acid and a crosslinker in order to achieve be...
Article
Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a...
Article
Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a...
Article
Full-text available
The application of lipases as catalysts in chemical reactions has been deterred by the high cost of isolation and purification of enzymes, the instability of their structure when they are isolated from their natural environment, contamination of products with residual protein, their sensitivity to process conditions, etc. These problems could be ov...
Article
Full-text available
Lipaze (triacilglicerol acil-hidrolaze, E.C. 3.1.1.3) su enzimi koji imaju sposobnost da katalizuju cepanje kar-boksilne veze estara, tri-, di-i monoacilglicerola do gli-cerola i masnih kiselina. Nalaze se u organizmima živo-tinja, biljaka i mikroorganizama u kojima igraju ključnu ulogu u metabolizmu lipida. Lipaze su od velikog zna-čaja za industr...

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Projects

Projects (3)
Project
The goal of this Project will be to synthetize and characterize biopolymer-based conjugates that could be deployed as substitutes for the commercially available methods for detecting and visualizing latent fingermarks, with little training and no operator hazards.
Project
Syntheses of novel carriers for encapsulation of different active substances. The production of new/improved biocatalysts. Controlled targeted release of encapsulated/immobilized substances. Enhancement of food quality, safety and competitiveness.