Nikola Hanišáková

Nikola Hanišáková
Masaryk University | MUNI · Department of Experimental Biology

Ph.D

About

10
Publications
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Introduction
- PhD student in Microbiology at Masaryk university - environmental microbiology - interested in anaerobic microorganisms, methanogenic archaea, cultivation techniques but also molecular biology methods

Publications

Publications (10)
Article
Full-text available
In recent years, there has been a growing interest in extending the potential of underground gas storage (UGS) facilities to hydrogen and carbon dioxide storage. However, this transition to hydrogen storage raises concerns regarding potential microbial reactions, which could convert hydrogen into methane. It is crucial to gain a comprehensive under...
Article
Full-text available
Securing new sources of renewable energy and achieving national self-sufficiency in natural gas have become increasingly important in recent times. The study described in this paper focuses on three geologically diverse underground gas reservoirs (UGS) that are the natural habitat of methane-producing archaea, as well as other microorganisms with w...
Article
Full-text available
As a renewable energy carrier, dark fermentative biohydrogen (H2) represents a promising future alternative to fossil fuels. Recently, the limited H2 yield of 4 moles of H2 per mole glucose, the so-called “Thauer limit”, was surpassed by a defined artificial consortium. In this article, we demonstrate the upscaling of this drawing board design, fro...
Article
Full-text available
The cultivation and investigation of strictly anaerobic microorganisms belong to the fields of anaerobic microbial physiology, microbiology, and biotechnology. Anaerobic cultivation methods differ from classic microbiological techniques in several aspects. The requirement for special instruments, which are designed to prevent the contact of the spe...
Preprint
Full-text available
Chemical modifications of RNA affect essential properties of transcripts, such as their translation, localization and stability. 5-end RNA capping with the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD+) has been discovered in organisms ranging from bacteria to mammals. However, the hypothesis that NAD+ capping might be universal...
Article
Full-text available
Employing deep reservoirs as UGS (underground gas storage) has a long history across continents. In 2018, 689 underground gas reservoirs with a total volume of 417 bcm were in operation worldwide. It is known that many microbial processes take place in the deep underground, even under the conditions of underground gas reservoirs. In this review, we...
Article
Full-text available
Over the past decades, anaerobic biotechnology is commonly used for treating high-strength wastewaters from different industries. This biotechnology depends on interactions and cooperation between microorganisms in the anaerobic environment where many pollutants' transformation to energy-rich biogas occurs. Properties of wastewater vary across indu...
Article
Full-text available
Spent coffee ground (SCG) may affect wastewater treatment processes due to high coffee consumption worldwide. The impact of the main chemical compounds present in SCG on respiration activity of sewage sludge was investigated. The results showed approximately two times higher respiration in the samples where various types of SCG were present in comp...

Questions

Question (1)
Question
This question is targered mostly on these who have worked with this instrument.
We‘ve conducted the experiments according to WTW Application report 0600412e „Determination of the biological biodegradability of organic substances under anaerobic conditions using the OxiTop® Control measuring system“. Our target for now is to adjust the conditions and obtain similar results; understanding of the mechanism and calculations is important for further experimenting. But there are points that need to be solved.
Of course the conditions can’t be fully imitated, there are many variables and the main is the volume of bottle which make 1 l.
At first, calculation of carbon content in the gasous phase require difference of pressure formed minus pressure in the beginning minus difference of blank. My question is, why there is the need of use blank and pressure at the beginning when theoretically the beginning pressure is included in blank variation (or where excatly should one choose the beginning).
The thing we don’t understand is the coefficient of total degradation Dt (or the meaning of it). Although biological degradation Dh corresponds with degradation of used amount of glucose, the mentioned Dt results in numbers that exceed 100% (even to 160% and higher).
If the problem doesn’t reside in the expelling of gasses from liquid phase by HCl. I use boiled tap water and then degass it with N2 to reduce CO2 in the water but the question is, how much is gas expel with HCl necesarry and influence the real results of methane production?
In the end, I would like to ask you about your experience with the use of anaerobic OxiTop and how you deal with calculating the produced methane gas.
Thank you.

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