Nikhil Ghate

Nikhil Ghate
University of Southern California | USC · Norris Comprehensive Cancer Center

Ph.D. Biochemistry

About

45
Publications
14,974
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
715
Citations
Citations since 2016
21 Research Items
642 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Additional affiliations
December 2010 - August 2016
Bose Institute
Position
  • Senior Researcher

Publications

Publications (45)
Article
Full-text available
Melanoma is a type of skin cancer that develops in pigment-producing melanocytes and often spreads to other parts of the body. Aberrant gene expression has been considered as a crucial step for increasing the risk of melanomagenesis, but how chromatin reorganization contributes to this pathogenic process is still not well understood. Here we report...
Article
Excess iron causes oxidative damage of biomolecules, leading to tissue injury primarily liver tissue causing liver failure. In this study, we explored the remediating effects of Morus alba L. (MAME) on iron-overload-induced oxidative stress and liver injury in mice. The In vitro study revealed the antioxidant and free radical scavenging properties...
Article
Full-text available
Histone modification is aberrantly regulated in cancer and generates an unbalanced state of gene transcription. VprBP, a recently identified kinase, phosphorylates histone H2A on threonine 120 (T120) and is involved in oncogenic transcriptional dysregulation; however, its specific role in colon cancer is undefined. Here, we show that VprBP is overe...
Article
Full-text available
p53 is a sequence‐specific transcription factor, and proper regulation of p53 transcriptional activity is critical for orchestrating different tumor‐suppressive mechanisms. p32 is a multifunctional protein which interacts with a large number of viral proteins and transcription factors. Here, we investigate the effect of p32 on p53 transactivation a...
Article
Full-text available
Background: MMP-9-dependent proteolysis of histone H3 N-terminal tail (H3NT) is an important mechanism for activation of gene expression during osteoclast differentiation. Like other enzymes targeting their substrates within chromatin structure, MMP-9 enzymatic activity toward H3NT is tightly controlled by histone modifications such as H3K18 acety...
Article
The search for new plant-based anti-inflammatory drugs continues in order to overcome the detrimental side effects of conventional anti-inflammatory agents, both steroidal and nonsteroidal. This study involves the quinoline SPE2, 7-hydroxy-6-methoxyquinolin-2(1H)-one, isolated from the EtOAc fraction of Spondias pinnata bark. Structure elucidation...
Chapter
Oxidative stress is associated with increased production of reactive oxygen species (ROS) that can pose a threat to cells by causing lipid peroxidation, protein oxidation, nucleic acid damage, enzyme inhibition and activation of cell death pathways. An uncontrollable production of ROS may lead to organ dysfunction and diseases. It has been well doc...
Article
Full-text available
Terminalia belerica Roxb. fruits have been previously reported against diabetes, ulcer, microbial problems and hepatotoxicity. The present study was aimed to investigate antioxidant and anticancer potential of sequentially fractionated hexane (TBHE), chloroform (TBCE), ethyl acetate (TBEE), butanol (TBBE) and water (TBWE) extracts from the 70% meth...
Article
Full-text available
Background: Iron in the overloaded condition in liver promotes the overproduction of free radicals that lead to oxidative stress and ultimately hepatic damage. The present study was designed to evaluate the ameliorating potential from iron overloaded hepatotoxicity by the glycosidic fraction from Spondious pinnata bark (SPW1) along with its antiox...
Article
Full-text available
Background Crude Spondias pinnata bark extract was previously assessed for its antioxidant, anticancer and iron chelating potentials. The isolated compounds gallic acid (GA) and methyl gallate (MG) were evaluated for their curative potential against iron overload-induced liver fibrosis and hepatocellular damage. Methods In vitro iron chelation pro...
Article
Full-text available
Background: Euglena tuba, a microalga, is known for its excellent antioxidant and iron-chelation activities; however its anticancer efficacies have not been reported yet. This study investigates the antitumor and antimetastatic activities of 70 % methanolic extract of Euglena tuba (ETME) against human lung (A549) and breast cancer (MCF-7) cells in...
Article
The first examples of Ru (II) and Ir (III) metalla-rectangles [1]4+-[4]4+ containing a BODIPY-based linker are reported; some of these compounds exhibited highly selective anticancer activity and interact strongly with DNA as well as protein. The characteristic green fluorescence of BODIPY ligand and associated aggregation-induced emission (AIE), p...
Article
Full-text available
The worldwide cancer incidences are remarkable despite the advancement in cancer drug discovery field, highlighting the need for new therapies focusing on cancer cell and its microenvironment, including inflammation. Several species of Drosera (family: Droseraceae) are used in various traditional as well as homeopathic systems of medicine. Drosera...
Article
Full-text available
The antioxidant and restoration potentials of hepatic injury by Prunus nepalensis Ser. (Steud), a wild fruit plant from the Northeastern region of India, were investigated. The fruit extract (PNME) exhibited excellent antioxidant and reducing properties and also scavenged the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC50 = 30.92 ± 0.40 μg/ml)....
Article
Full-text available
Free iron typically leads to the formation of excess free radicals, and additional iron deposition in the liver contributes to the oxidative pathologic processes of liver disease. Many pharmacological properties of the insectivorous plant Drosera burmannii Vahl. have been reported in previous studies; however, there is no evidence of its antioxidan...
Article
Full-text available
Liver toxicity due to iron overload leads to oxidative damage of proteins, lipids and nucleic acids which in turn manifests several human diseases. Here, we evaluated the improving effect of Clerodendrum colebrookianum leaf on iron overload induced liver injury along with in vitro iron chelation and the protection of Fenton reaction induced DNA dam...
Article
Spondias pinnata has been reported for its efficient anticancer effects, but the studies were mostly focused on its extract. Since its bioactive compounds are largely unknown, this study was designed to characterize the lead components present in it and their anticancer activity against human glioblastoma cell line (U87). Major compounds from the e...
Article
Context: Nerium indicum Mill. (Apocynaceae) was reported for its efficient in vitro antioxidant and iron-chelating properties. Objective: This study demonstrates the effect of 70% methanol extract of N. indicum leaf (NIME) towards in vitro DNA protection and ameliorating iron-overload-induced liver damage in mice. Materials and methods: Phytoc...
Article
Iron overload in liver is associated with the oxidative damage of protein, lipid and nucleic acids which in turn manifests several human diseases. The present study was aimed to evaluate the amending effect of 70% methanol extract of Hemidesmus indicus root (HIME) on iron overload induced liver injury. Iron overload was induced by intraperitoneal a...
Conference Paper
Free radicals are formed naturally in the body and play an important role in many normal cellular processes. Sometimes, several internal metabolic processes or external stimuli (UV, radiation, ozone) may lead to the production of high concentrations of free radicals. These abnormally high concentrations of free radicals are hazardous to major compo...
Conference Paper
Full-text available
T he use of herbal based medicine is gaining importance worldwide and medicinal plants are believed to be an important source of new lead chemicals with potential therapeutic effects. Free radicals play a key role in many degenerative diseases such as cardiovascular disease, cataracts, weak immune system, brain dysfunction and cancer. Antioxidants...
Article
AimsThe present study is aimed to evaluate different phytochemical constituents and the ameliorating effect of 70% methanol extract of Euglena tuba (ETME) on iron overload induced liver injury, along with its in vitro iron chelating and DNA protection effects.Methods and ResultsPhytochemicals of ETME were identified by GCMS analysis. Iron chelation...
Conference Paper
Full-text available
M edicinal values of plants have been recognized owing to the presence of biologically active phytochemicals beneficial to the human body. Most of the physiological impairment, tissues damages, pathological events or diseases have been attributed to unstable and extremely reactive chemical species called free radicals and/or reactive oxygen species...
Article
In vitro assessment of phytochemicals, antioxidant and DNA protective potential of wild edible fruit of Elaeagnus latifolia Linn. Abstract – Introduction. Elaeagnus latifolia Linn. is a type of wild edible fruit found in nor-theast India, Thailand and also in Vietnam. Although the fruit is reported to be a source of vita-mins, minerals, essential f...
Article
Full-text available
Background:Unstable generation of free radicals in the body are responsible for many degenerative diseases. A bloom forming algaeEuglena tubagrowing abundantly in the aquatic habitats of Cachar district in the state of Assam in North-East India was analysed for its phytochemical contents, antioxidant activity as well as free radical scavenging pote...
Article
The heartwood extract of A. catechu, called pale catechu or "Katha" in Hindi has been widely used in traditional Indian medicinal system. Although various pharmacological properties of this plant had been reported previously, only a few were concerned with the anticancer activity of this plant. The objective was to assess the in vitro anticancer an...
Article
The objective of the present study was to assess the in vitro anticancer activity of 70% methanolic extract of Terminalia belerica (TBME) against human lung (A549) and human breast (MCF-7) carcinoma and its possible mechanism. TBME showed significant cytotoxicity to both A549 and MCF-7 cells, whereas, no cytotoxicity was found in non-malignant WI-3...
Article
Full-text available
The medicinal role of dietary plants in promoting the healthy endurance of human beings is traditionally well documented through ancient and modern times all over the world. The purpose of this study was to delineate the differential antioxidant, ROS scavenging and iron chelation activities of the 70% methanolic extract (DALM) and aqueous extract (...
Conference Paper
Full-text available
A number of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are essential parts of aerobic life and metabolism. ROS and RNS are normally generated by tightly regulated enzymes, such as NAD(P)H oxidase isoforms and NO synthase (NOS), respectively. Free radicals increase tumorigenesis by causing DNA damage and mutation, inhibiting a...
Article
This report highlights the phytochemical analysis, antioxidant potential and anticancer activity against breast carcinoma of 70% methanolic extract of lichen, Parmotrema reticulatum (PRME). Phytochemical analysis of PRME confirms the presence of various phytoconstituents like alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, tann...
Article
Full-text available
Objective: The present study was undertaken, to evaluate the in vitro antioxidant capacity as well as phytochemical quantification of Clerodendrum colebrookianum. Methods: A 70% methanolic extract (CCLM) and aqueous extract (CCLA) of C. colebrookianum Walp. leaves to evaluate different in vitro tests in diversified fields including the total antiox...
Article
Spondias pinnata, a commonly distributed tree in India, previously proven for various pharmacological properties and also reported for efficient anti-oxidant, free radical scavenging and iron chelating activity, continuing this, the present study is aimed to investigate the role of 70 % methanolic extract of S. pinnata bark (SPME) in promoting apop...
Article
This study evaluated the ameliorating effect of 70% methanol extract of Acacia catechu heartwood, or Katha (ACME) on liver injury induced by iron overload. Iron overload in mice was caused by intraperitoneal administration of 100 mg/kg iron-dextran. ACME was administered orally for 21 days, starting from the day after the first iron-dextran injecti...
Article
This study evaluated the ameliorating effect of 70% methanol extract of Acacia catechu heartwood, or Katha (ACME) on liver injury induced by iron overload. Iron overload in mice was caused by intraperitoneal admin-istration of 100 mg/kg iron-dextran. ACME was administered orally for 21 days, starting from the day after the first iron-dextran inject...
Article
Antioxidants have reactive oxygen species (ROS) scavenging ability with great relevance in the prevention of oxidative stress. The present study aimed to examine the antioxidant and ROS scavenging activities of 70% methanolic extract of Tinospora cordifolia. The plant was found a significant source of carbohydrate, alkaloid, ascorbic acid, tannin,...
Article
Withania somnifera, also known as 'Ashwagandha', is an important medicinal plant that has been used in Ayurvedic and indigenous medicine for over 3,000 years. In view of its varied therapeutic potential, it has also been the subject of considerable modern scientific attention. The present study was performed to evaluate the phytochemicals and in vi...

Questions

Questions (3)
Question
Do I need to add a stopping codon when expressing protein in pET 15b vector?
Question
I have tried 0.5% but couldnt separate it.
Question
For anti-inflammatory studies I need them..

Network

Cited By