Nicolay Rustad Nilssen

Nicolay Rustad Nilssen
Thermo Fisher Scientific | TFS · Cellular medicine

PhD

About

9
Publications
828
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40
Citations
Citations since 2016
7 Research Items
36 Citations
20162017201820192020202120220246810
20162017201820192020202120220246810
20162017201820192020202120220246810
20162017201820192020202120220246810

Publications

Publications (9)
Article
Full-text available
Phage display screening readily allows for the identification of a multitude of antibody specificities, but to identify optimal lead candidates remains a challenge. Here, we direct the antibody-capsid fusion away from the signal sequence-dependent secretory SEC pathway in E. coli by utilizing the intrinsic signal sequence-independent property of pI...
Article
Full-text available
Phage display has been instrumental in discovery of novel binding peptides and folded domains for the past two decades. We recently reported a novel pIX phagemid display system that is characterized by a strong preference for phagemid packaging combined with low display levels, two key features that support highly efficient affinity selection. Howe...
Article
Full-text available
There is a quest for production of soluble protein of high quality for the study of T-cell receptors (TCRs), but expression often results in low yields of functional molecules. In this study, we used an E. coli chaperone-assisted periplasmic production system and compared expression of 4 different soluble TCR formats: single-chain TCR (scTCR), two...
Data
Amino acid sequences of the TCR constructs. The amino acid sequence of (A) scTCR wt380, (B) scTCR s380, (C) dsTCR 380, (D) ct-dsTCR 380 and (E) cFab 380. In all variants the leader sequence is highlighted in light grey, variable domains in white, linker in teal and c-Myc and His6 tags in violet. The introduced stabilizing mutations of scTCR s380 (B...
Data
Expression characteristics of the recombinant TCR formats. Representative western blot showing the expression profile of the TCR formats. E. coli RosettaBlueTM expression cultures were normalized and fractionated into; M, medium; P, periplasmic; C, cytosolic fractions before analysis by western blots (n = 3–4). All samples were detected with anti-H...
Data
Original uncropped western blots. Fig 2A are composed of the following three (A, B, C) uncropped original western blots. (TIF)

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