Nicolas Richet

• PhD of Plant Biology
• Project Manager at Comité Champagne - CIVC

Efficient early pathogen detection, before symptom apparition, is crucial for optimizing disease management. In barley, the fungal pathogen Pyrenophora teres is the causative agent of net blotch disease, which exists in two forms: P. teres f. sp. teres (Ptt), causing net-form of net blotch (NTNB), and P. teres f. sp. maculata (Ptm), responsible for...

Net blotch disease caused by Drechslera teres is a major fungal disease that affects barley (Hordeum vulgare) plants and can result in significant crop losses. In this study, we developed a deep-learning model to quantify net blotch disease symptoms on different days post-infection on seedling leaves using Cascade R-CNN (Region-Based Convolutional...

Pommery Champagne cellar has the originality to be in the underground of Reims city (NE - France) and has got monumental bas-reliefs carved in chalk walls. Bas-reliefs were illuminated for tourist visits and led to a lampenflora growth with different coloured areas which mar the beauty of the artworks. This study was focused on the characterization...

Wheat and barley rank among the main crops cultivated on a global scale, providing the essential nutritional foundation for both humans and animals. Nevertheless, these crops are vulnerable to several fungal diseases, such as Septoria tritici blotch and net blotch, which significantly reduce yields by adversely affecting leaves and grain quality. T...

Neofusicoccum parvum is one of the most aggressive Botryosphaeriaceae species associated with grapevine trunk diseases. This species may secrete enzymes capable of overcoming the plant barriers, leading to wood colonization. In addition to their roles in pathogenicity, there is an interest in taking advantage of N. parvum carbohydrate-active enzyme...

Purpose This study aimed to evaluate the radiosensitizing potential of Au@DTDTPA(Gd) nanoparticles when combined with conventional external X-ray irradiation (RT) to treat GBM. Methods Complementary biological models based on U87 spheroids including conventional 3D invasion assay, organotypic brain slice cultures, chronic cranial window model were...

Background Grapevine trunk diseases (GTDs) are a complex group of diseases that lead to major economic losses in all wine‐producing countries. The investigation of biocontrol agents (BCAs) capable of forestalling or at least minimizing the development of GTDs has, recently, become a priority. Nursery experiments were set up to (i) assess the biocon...

Controlling crown gall in grapevine ( Vitis vinifera L.) caused by the pathogenic bacterium Allorhizobium vitis is a major challenge for global viticulture, as this pathogen is highly persistent in vineyards once infected. The bacteria can enter the plant through open wounds during pruning and then systemically colonize the plant. This study aimed...

The use of natural bio-based compounds becomes an eco-friendly strategy to control plant diseases. Rare sugars would be promising compounds as inducers of plant “sweet immunity”. The present study aimed to investigate the induced resistance of grapevine leaves against Plasmopara viticola and Botrytis cinerea by a rare sugar-based product (IFP48) an...

Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this st...

Main conclusion Depending on the N source and plant ontogenetic state, the epiphytic tank-forming bromeliad Vriesea gigantea can modulate aquaporin expression to maximize the absorption of the most available nitrogen source. Abstract Epiphytic bromeliads frequently present a structure formed by the overlapping of leaf bases where water and nutrien...

The sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: the Nodulin26‐like Intrinsic Protein (NIP) and BOR transporter family. Though the agriculturally important crop Brassica napus is highly sensitive to B deficiency and NIPs and BORs have been sugges...

Aquaporins (AQPs) are tetrameric channel proteins regulating the transmembrane flux of small uncharged solutes and in particular water in living organisms. In plants, members of the plasma membrane intrinsic protein (PIP) AQP subfamily are important for the maintenance of the plant water status through the control of cell and tissue hydraulics. The...

Trees adjust their growth following forced changes in orientation, to re-establish a vertical position. In angiosperms this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formati...

Forest trees cover 30% of the earth's land surface, providing renewable fuel, wood, timber, shelter, fruits, leaves, bark, roots, and are source of medicinal products in addition to benefits such as carbon sequestration, water shed protection, and habitat for 1/3 of terrestrial species. However, the genetic analysis and breeding of trees has lagged...

Gravity is one of the most important environmental parameter affecting plant growth and development. In response to such mechanical stimulus trees constantly remodel their position by developing a specialized wood called reaction wood. In angiosperms trees, such as poplars, reaction wood is named tension wood (TW) and is localized on upper side of...

Trees will have to cope with increasing levels of CO2 and ozone in the atmosphere. The purpose of this work was to assess whether the lignification process could be altered in the wood of poplars under elevated CO2 and/or ozone. Young poplars were exposed either to charcoal-filtered air (control), to elevated CO2 (800 μl l−1), to ozone (200 nl l−1)...

Ozone induces a stimulation of the phenylpropanoid and lignin biosynthesis pathways in leaves but the response of wood, the main lignin-producing tissue, is not well documented. The purpose of this study was to compare the responses of phenylpropanoid and lignin pathways in leaves and stem wood by a simultaneous analysis of both organs. Young popla...

Wood formation is altered in poplar under ozone and/or carbon dioxide.

Wood formation in trees is a dynamic process that is strongly affected by environmental factors. However, the impact of ozone on wood is poorly documented. The objective of this study was to assess the effects of ozone on wood formation by focusing on the two major wood components, cellulose and lignin, and analysing any anatomical modifications. Y...

In the context of global climate change, trees will have to cope with an atmosphere different from actual. Ozone and CO2 are two of the main gas whose increased concentration is worrying. We investigated the effects of ozone and elevated CO2 single or combined on the biosynthesis and content of lignin, wood anatomy and properties of young poplars (...