Nicolas Kylilis

Nicolas Kylilis
University of Cyprus · Department of Biological Sciences

PhD in Synthetic Biology & Molecular Biology

About

13
Publications
6,739
Reads
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275
Citations
Introduction
My current work focuses on the the development of a biosensor platform -of synthetic biology design- for the detection of protein biomarkers in the bloodstream that are of clinical importance.
Additional affiliations
March 2017 - present
Imperial College London
Position
  • PostDoc Position
Description
  • Postdoctoral Research Associate in Synthetic Biology
July 2016 - October 2016
Imperial College London
Position
  • Project Manager
Description
  • Project Manager of research team of Imperial College for the 2016 iGEM competition
October 2012 - January 2017
Imperial College London
Position
  • PhD Student
Description
  • Synthetic Biology Biosensor Design for Personal Diagnostics
Education
October 2012 - January 2017
Imperial College London
Field of study
  • Synthetic Biology / Molecular Biology
September 2011 - September 2012
Imperial College London
Field of study
  • Systems & Synthetic Biology
September 2008 - August 2011
Imperial College London
Field of study
  • Biochemistry

Publications

Publications (13)
Article
Full-text available
Whole-cell biosensors can form the basis of affordable, easy-to-use diagnostic tests that can be readily deployed for point-of-care (POC) testing, but to date, the detection of analytes such as proteins that cannot easily diffuse across the cell membrane has been challenging. Here we developed a novel biosensing platform based on cell agglutination...
Article
Full-text available
Advancing synthetic biology to the multicellular level requires the development of multiple cell-to-cell communication channels that propagate information with minimal signal interference. The development of quorum-sensing devices, the cornerstone technology for building microbial communities with coordinated system behaviour, has largely focused o...
Preprint
Full-text available
Affordable, easy-to-use diagnostic tests that can be readily deployed for point-of-care (POC) testing are key in addressing challenges in the diagnosis of medical conditions and for improving global health in general. Ideally, POC diagnostic tests should be highly selective for the biomarker, user-friendly, have a flexible design architecture and a...
Article
Full-text available
Native cell-free transcription–translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host, Bacillus megaterium, is a giant Gram-positive bacterium with pote...
Preprint
Full-text available
Advancing synthetic biology to the multicellular level requires the development of multiple orthogonal cell-to-cell communication channels to propagate information with minimal signal interference. The development of quorum sensing devices, the cornerstone technology for building microbial communities with coordinated system behaviour, has largely...
Preprint
Automation and factorial experimental design together with cell-free in vitro transcription-translation systems offers a new route to the precise characterization of regulatory components. This now presents a new opportunity to illuminate the genetic circuitry from arcane microbial chassis, which are difficult to assess in vivo . One such host, Bac...
Article
Full-text available
Parasitic diseases affect millions of people worldwide, causing debilitating illnesses and death. Rapid and cost-effective approaches to detect parasites are needed, especially in resource-limited settings. A common signature of parasitic diseases is the release of specific proteases by the parasites at multiple stages during their life cycles. To...
Article
Full-text available
The use of biological entities as 'sensors' has long been of interest to engineers and biologists. With recent advances in our ability to engineer biology, potential biosensors can be identified, engineered and tested faster than ever before. In this chapter, we discuss different types of biosensors and their potential uses and the considerations a...

Questions

Question (1)
Question
I have carried out a His-tag assisted affinity purification of a protein (43kDa) and now I am trying to buffer exchange and concentrate down my protein sample using a Vivaspin 20ml concentrator (30K MWCO) to concentrate down my protein. However it takes too long for the buffer to pass through the filter (1h-1h30min). Does anyone know whether this signifies that something is wrong with the protein sample?
Thanks in advance for your time!
P.S. I know I should have used a concentrator with lower MWCO but I wanted to get rid off a 27kDa protein that co-purifies with my protein.

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Projects

Projects (2)
Project
Design principles for microbial-based sensing devices