Nicolas Cifuentes-Muñoz

Nicolas Cifuentes-Muñoz
University of Kentucky | UKY · Department of Biochemistry

Ph.D. in Biotechnology

About

17
Publications
3,208
Reads
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346
Citations
Introduction
Currently working at the University of Kentucky as a postdoctoral scholar. I am focused in studying the replication and assembly of human metapneumovirus and Hendra virus.
Additional affiliations
October 2014 - October 2018
University of Kentucky
Position
  • PostDoc Position
Education
March 2006 - April 2011
Universidad Andrés Bello
Field of study
  • Biotechnology
March 1999 - April 2005
University of Santiago, Chile
Field of study
  • Biochemistry

Publications

Publications (17)
Article
Full-text available
Human metapneumovirus (HMPV) inclusion bodies (IBs) are dynamic structures required for efficient viral replication and transcription. The minimum components needed to form IB-like structures in cells are the nucleoprotein (N) and the tetrameric phosphoprotein (P). HMPV P binds to the following two versions of the N protein in infected cells: N-ter...
Preprint
Full-text available
Human metapneumovirus (HMPV) inclusion bodies (IBs) are dynamic structures required for efficient viral replication and transcription. The minimum components needed to form IB-like structures in cells are the nucleoprotein (N) and the tetrameric phosphoprotein (P). HMPV P binds to two versions of N protein in infected cells: C-terminal P residues i...
Chapter
A critical step in the life cycle of a virus is spread to a new target cell, which generally involves the release of new viral particles from the infected cell which can then initiate infection in the next target cell. While cell-free viral particles released into the extracellular environment are necessary for long distance spread, there are disad...
Article
Pneumoviruses represent a major public health burden across the world. Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV), two of the most recognizable pediatric infectious agents, belong to this family. These viruses are enveloped with a non-segmented negative-sense RNA genome, and their replication occurs in specialized cytosolic...
Article
Full-text available
Human metapneumovirus (HMPV) causes significant upper and lower respiratory disease in all age groups worldwide. The virus possesses a negative-sense single-stranded RNA genome of approximately 13.3 kb encapsidated by multiple copies of the nucleoprotein (N), giving rise to helical nucleocapsids. In addition, copies of the phosphoprotein (P) and th...
Article
Full-text available
Hendra virus (HeV) is a zoonotic paramyxovirus that causes deadly illness in horses and humans. An intriguing feature of HeV is the utilization of endosomal protease for activation of the viral fusion protein (F). Here we investigated how endosomal F trafficking affects HeV assembly. We found that the HeV matrix (M) and F proteins each induced part...
Article
Full-text available
Paramyxovirus spread generally involves assembly of individual viral particles which then infect target cells. We show that infection of human bronchial airway cells with human metapneumovirus (HMPV), a recently identified paramyxovirus which causes significant respiratory disease, results in formation of intercellular extensions and extensive netw...
Data
Proteomic Analysis of Purified HMPV Particles. (DOCX)
Data
Proteomic Analysis of Exosomes Purified from Mock Infected Cells. (DOCX)
Article
Full-text available
In recent years, ultrastructural studies of viral surface spikes from three different genera within the Bunyaviridae family have revealed a remarkable diversity in their spike organization. Despite this structural heterogeneity, in every case the spikes seem to be composed of heterodimers formed by Gn and Gc envelope glycoproteins. In this review,...
Article
Full-text available
How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle forma...
Article
Full-text available
Human Papillomavirus (HPV) is the etiological agent of cervical cancer. Like other oncogenic viruses, HPV encode oncoproteins whose role in cellular immortalization and transformation has been extensively investigated. HPV E6 and E7 proteins disable tumor suppressors p53 and Rb and up-regulate telomerase, fundamental changes for cell immortalizatio...
Article
Full-text available
Deformed wing virus (DWV) is one of the most common viruses affecting honey bee specimens. Although the presence of DWV has been reported in many countries, there is no data of the current situation in Chile. In this report, we detected the presence of DWV in apiaries from two different locations in central Chile. Furthermore, the genome of a Chile...
Article
Full-text available
Hantaviruses infect human cells through cell attachment and subsequent fusion of viral and cellular membranes at low pH. This largely unknown entry process is mediated by the Gn and Gc glycoproteins, anchored at the viral envelope membrane. Performing bioinformatic analysis and peptide-liposome-binding assays we suggested in a former report that Gc...
Article
Full-text available
To infect target cells, enveloped viruses use their virion surface proteins to direct cell attachment and subsequent entry via virus-cell membrane fusion. How hantaviruses enter cells has been largely unexplored. To study early steps of Andes virus (ANDV) cell infection, a lentiviral vector system was developed based on a Simian immunodeficiency vi...

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Projects

Project (1)
Project
To elucidate the mechanism of genome transcription/replication and subsequent transportation of nucleocapsids to assembly sites