Nicola Fiore

Nicola Fiore
University of Chile · Facultad de Ciencias Agronómicas, Department of Sanidad Vegetal

PhD

About

72
Publications
15,501
Reads
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719
Citations
Citations since 2016
40 Research Items
459 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Additional affiliations
January 2008 - present
University of Chile
Education
March 2005 - May 2009
University of Chile
Field of study
  • Plant pathology

Publications

Publications (72)
Article
Full-text available
Pistachio crops have a great economic potential, as their global production has increased dramatically over the past few decades. Therefore, it is important to maintain the healthy phytosanitary status of pistachio crops. In a Chilean pistachio orchard, a dieback of the trees was observed, with blighting of twigs and severe necrosis in the trunk an...
Article
Full-text available
Tomatoes are susceptible to bacterial diseases, mainly related to some Pseudomonas syringae pathovars. Many Pseudomonas species are considered innocuous, but some have shown the ability to opportunistically infect tomato plants. Antimicrobial compounds have been used to control pathogenic organisms, and this can lead to environmental selection of p...
Article
The genus ‘Candidatus Phytoplasma’ was proposed to accommodate cell wall-less bacteria that are molecularly and biochemi- cally incompletely characterized, and colonize plant phloem and insect vector tissues. This provisional classification is highly relevant due to its application in epidemiological and ecological studies, mainly aimed at keeping...
Article
Full-text available
Phylogenetic analyses categorize seven genotypes of citrus tristeza virus (CTV). The symptoms caused by this pathogen, their expression and severity are influenced by CTV genotypes, host species, cultivars, and infected host rootstocks. This study aimed to verify how populations of Chilean CTV isolates changed following inoculation from infected sw...
Article
Full-text available
The considerable economic losses in citrus associated with ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ presence have alerted all producing regions of the world. In Chile, none of these bacteria have been reported in citrus species. During the years 2017 and 2019, 258 samples presenting symptoms similar to those associated with the presen...
Article
Full-text available
Grapevine rupestris stem pitting associated virus (GRSPaV) is one of the most widely distributed viruses; even so, little is known about its effect on Vitis vinifera. To provide new insights, the effects of single and mixed GRSPaV infections on the V. vinifera cultivar “Cabernet Sauvignon” were studied by evaluating growth parameters, such as measu...
Article
Phytoplasmas are bacteria belonging to class Mollicutes that are able to infect a wide diversity of plant crops, causing significant economic losses worldwide. During the last decade, the typical symptoms exhibited by phytoplasma infected plants, like phyllody, excessive shoot proliferation and loss of apical dominance have been associated to the s...
Article
Full-text available
To date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the country were sampled and analyzed by nested PCR/RFL...
Chapter
Full-text available
The impact of phytoplasmas in agriculture has become serious, and early diagnosis is the best option to prevent the disease spread. Very often the symptoms-based diagnostics is not sufficient or able to discriminate among the diverse phytoplasmas. Until the early 1980s, the phytoplasma presence in diseased plants was detected by transmission electr...
Article
Full-text available
Since the year 2015, strawberry plants with symptoms of phyllody, achenes’ hypertrophy and leaf reddening have been observed in several fields in various regions of Chile. In recent surveys on five individual fields in Biobío and Araucanía regions, samples were collected from symptomatic plants and DNA was extracted. Two genomic regions correspondi...
Chapter
To reduce the spread of phytoplasmas in a crop or in a certain geographic area, epidemiological studies are of crucial importance in determining which insect species transmit these pathogens. In this chapter, we describe methods of capturing the insect vectors of phytoplasmas and the criteria for choosing the method(s) according to the objective to...
Chapter
The diseases associated with phytoplasmas in grapevine are collectively called yellows and occur in the majority of grapevine-growing regions over the world. At first, a short overview of symptoms and damage associated with the presence of grapevine phytoplasmas is reported. Then, vectors, alternative host plants, and epidemiological cycles, where...
Chapter
The present chapter focuses on phytoplasmas and phytoplasma diseases affecting stone, pome, and small fruit worldwide. An outlook is also provided on other fruit tree species growing mainly in tropical and subtropical areas that are oftern infected by phytoplasma diseases usually associated with crop losses or loss of fruit quality and marketabilit...
Book
Provides recent and updated information on emerging and re-emerging phytoplasma diseases affecting important crops in tropics and subtropics Provides comprehensive information on disease distribution, occurrence, and identification of the phytoplasmas Includes the recent approaches for diagnostics, transmission, and information about losses and geo...
Article
Full-text available
During spring 2016 commercial lettuce and Swiss chard plants, exhibiting disease symptoms suggestive of possible phytoplasma infection, were collected together with asymptomatic plants in Metropolitana and Arica y Parinacota regions of Chile, respectively. Nucleic acids extracted from leaf midribs were used for phytoplasmas detection. Nested polyme...
Article
Kiwifruit bleeding sap samples, collected in Italian and Chilean orchards from symptomatic and asymptomatic plants, were evaluated for the presence of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker. The saps were sampled during the spring of both hemispheres, before the bud sprouting, during the optimal time window for th...
Presentation
24rd International Conference on Virus and Other Transmissible Disease of Fruit Crops. Grecia 2017.
Article
Stone fruits rank third among the most important crop species in Chile, after grapevine and apple. Specifically, cherry (Prunus avium L.) cultivation have increased during the last 10 years, making of Chile the most important exporter in the Southern hemisphere. Nineteen cherry samples collected in the spring of 2016 were subjected to high-throughp...
Article
Full-text available
Chile is free of the “flavescence dorée” disease and its vector Scaphoideus titanus. Given the importance of viticulture industry in the country, it is fundamental to know which could be the consequences in case of accidental insect introduction in the Country. The potential distribution of the vector was evaluated through a model using the BIOCLIM...
Article
Pear decline (PD) is one of the most devastating diseases that affects pear trees and it has been detected in the Mediterranean basin and in the United States. The causal agent is ‘Candidatus Phytoplasma pyri’ (‘Ca. P. pyri’), belonging to the apple proliferation group (16SrX), ribosomal subgroup C. Cacopsylla pyri L. has been found as the main vec...
Article
Full-text available
Evaluation of the prevalence of virus and viroid infections was conducted in a grapevine field collection in Valencia, Spain. Samples of autochthonous and traditional grapevine cultivars were collected during November 2011 and tested for the presence of fourteen viruses and five viroids, using RT-PCR. The prevalent viruses were Grapevine rupestris...
Article
Full-text available
Phytoplasmas are bacterial plant pathogens that can affect different vegetal hosts. In South America, a phytoplasma belonging to ribosomal subgroup 16SrIII-J has been reported in many crops. Here we report its genomic draft sequence, showing a total length of 687,253 bp and a G+C content of 27.72%.
Article
Full-text available
Tomato ringspot virus (ToRSV) has been detected in Chile, causing economically important diseases in a wide range of hosts. A ToRSV isolate was obtained from raspberry cv Heritage (Rasp-CL) showing leaf yellowing and stunting. The complete genome of Rasp-CL was sequenced by deep sequencing. The Rasp-CL RNA1 sequence shared 97.4 % nucleotide sequenc...
Article
Grapevine leafroll-associated virus 2 (GLRaV-2) is prevalent in Chile but no information is available on the genetic variability of Chilean isolates. Three genomic regions, i.e. a partial RNA-dependent RNA polymerase (RdRp) gene, the complete coat protein (CP) gene and a partial p19 gene fragment, of eleven Chilean isolates were used for phylogenet...
Article
Stone fruits orchards from three Chilean regions were visited to collect leaf samples which were tested for the presence of the most important viruses and viroids. Molecular nonradioactive hybridization (MH) testing of 2,456 samples and confirmatory RT-PCR of some MH-negative samples gave the following infection rates: 31.5% for Prunus necrotic rin...
Article
Full-text available
‘Candidatus Phytoplasma ulmi’ (Elm yellows, 16SrV-A), transmitted by Amplicephalus curtulus Linnavuori & DeLong (Hemiptera: Cicadellidae), has been found in native Chilean plants, and transovarial transmission has been considered as a possible form of transmission. An analysis to detect the presence of ‘Ca. Phytoplasma ulmi’ and other phytoplasmas...
Article
Nested polymerase chain reacti on assays usi ng phyt opl asma universal primer pairs P1A/P7A and R16F2n/ R2 det ect ed phyt opl asmas i n grapevine vari eties Syrah and Superior in Chile showing yellows symptoms. Cloning, sequencing, and in sili co restri ction fragment length polymorphism anal yses, al lowed identificat ion of phytoplasmas bel ong...
Article
Full-text available
Due to the sporadic occurrence of yellow wilt of sugar beet disease throughout the last three years, a survey was carried out in two regions where the Chilean sugar beet production is concentrated. The tuf and 16S rRNA genes were used for phytoplasma detection and identification. Virtual restriction fragment length polymorphism analyses, cloning, a...
Article
In a pear orchard of variety d'Anjou located in Maule Region (Chile) several Galega officinalis L. plants exhibited yellowing leaves. Nested polymerase chain reaction assays, using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 100% of symptomatic samples. Restriction fragment length polymorphism analyses, cloning,...
Article
The most common insect in Chilean phytoplasma-infected vineyards, belonging to the family Cicadellidae, is Paratanus exitiosus (Beamer). This leafhopper was proved to be able to transmit the 16SrIII-J phytoplasma to periwinkle plants. In the present work we demonstrate that P. exitiosus transmits the same phytoplasma to grapevine plants too.
Article
One of the most common insects in vineyards infected with phytoplasmas belonging to the family Cicadellidae, is Bergallia valdiviana Berg 1881 This leafhopper has not yet been described as a phytoplasma vector. The present work demonstrates that B. valdiviana is able to transmit phytoplasmas to periwinkle plants.
Article
In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed in a real-time PCR using a primer set for both of them o...
Article
Full-text available
Grapevine fanleaf virus (GFLV), a nematode-transmitted virus belonging to the genus Nepovirus, is the major pathogen responsible for fanleaf degeneration, one of the most widespread and damaging viral diseases of grapevine. The virus is characterized by a genome constituted by two single positive sense RNAs (RNA1 and RNA2), coding for two polyprote...
Article
Full-text available
Background Microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. The majority of the studies investigating gene expression changes in virus-infected plants are limited to interactions between a virus and a model host plant, which usually is Arabidop...
Article
Full-text available
In Chile, sweet cherry represents one of the most valuable fruit crops, and the country is the main producer of cherries in the southern hemisphere. In October 2011, leaf samples were collected from 21 trees of the cultivar Bing in Libertador General Bernardo O?Higgins and Maule regions. Leaves of symptomatic plants showed brown angular necrotic sp...
Article
Full-text available
Plants of murta (Ugni molinae Turcz.) and peony (Paeonia lactiflora Pall.), exhibiting disease symptoms suggestive of possible phytoplasma infection were observed in Chile. Leaves were collected from six plants per species (three with and three without symptoms) and main leaf midribs were used for phytoplasma testing. Nested polymerase chain reacti...
Article
Vineyards in the Atacama region in Chile were surveyed from 2007 to 2009 for the presence of viruses. This region is an important area of table grape production, supplying international markets with its fruits in the off season of the Northern Hemisphere. Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect the most eco...
Article
Full-text available
Some Auchenorrhyncha specimens were captured, identified and tested to verify phytoplasma presence in Chilean vineyards; many of them belong to the subfamily Delthocephalinae and Agalliinae (family Cicadellidae) and to the families Cixiidae and Delphacidae, all known as potential phytoplasma vectors. Several individuals were positives to phytoplasm...
Article
Full-text available
During summer 2006 sweet cherry (Prunus avium L.) trees, exhibiting disease symptoms suggestive of possible phytoplasma in-fection were observed in the Libertador General Bernardo O'Higgins region of Chile. Branches were collected from ten plants (five with and five without symptoms) and nucleic acid extracted from leaf midribs and phloem scrapings...
Article
Full-text available
During 2007, a survey was performed to detect and identify Plum pox virus (PPV) in Chilean stone fruit commercial orchards. A total of 1396 trees were analyzed and 45 (3.22 %) of them resulted positive. A fragment of 467 bp, corresponding to the replicase-coat protein (Nib-CP) region from the virus genome, was amplified and the the sequences obtain...
Article
At least 58 viruses have been reported to infect grapevines causing economic damage globally. Conventional detection strategies based on serological assays, biological indexing and RT-PCR targeting one or few viruses in each assay are widely used. Grapevines are prone to contain mixed infections of several viruses, making the use of these technique...
Article
An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, clo...
Article
The combined effect of thermotherapy and tissue culture was evaluated on Red Globe variety infected with Grapevine leafroll associated virus 2 Red Globe strain (GLRaV-2-RG). Five plants, derived from a positive mother stock plant to GLRaV-2-RG, previously verified by RT-PCR were subjected to 20, 40, 60, 80 and 100 days at 38ºC and 75% RH. In each p...
Article
Full-text available
Grapevine fanleaf virus (GFLV), Grapevine leafroll associated virus 1, 2, and 3 (GLRaV-1,-2, and -3), Grapevine virus A (GVA) and Grapevine fleck virus (GFkV) were monitored monthly throughout a year in naturally infected field-grown vines by ELISA and RT-PCR. The organs tested were: opening buds in September, tips or unfolded leaves in September a...
Article
Grapevine leafroll is one of the most widespread and economically relevant viral diseases of grapevines. At least nine distinct Grapevine leafroll-associated viruses (GLRaVs), all members of the Closteroviridae family, have been associated with this disease in grapevine. Grapevine leafroll-associated virus 4 (GLRaV-4), currently classified as a Clo...
Article
A method was developed for routine analysis to detect latent infection of Clavibacter michiganensis subsp. michiganensis in samples from tomato seedling lots, before transplant, using polymerase chain reaction. In samples of 300 stem segments 1 cm long, the sensitivity threshold of the method was estimated at around 1.1 × 103 corynebacteria or 0.33...
Article
Full-text available
Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine...
Article
Grapevine fanleaf virus (GFLV) is the causal agent of a widespread disease that affects vineyards. Since it is difficult to culture viruses, the availability of an easy and efficient method of virus maintenance in the laboratory would be of interest to virologists. The objective of this research was to determine an adequate culture medium that prom...
Article
Full-text available
Prunus necrotic ringspot virus (PNRSV) is distributed worldwide, but no molecular data have been previously reported from South American isolates. The nucleotide sequences corresponding to the movement (MP) and coat (CP) proteins of 23 isolates of PNRSV from Chile, Brazil, and Uruguay, and from different Prunus species, have been obtained. Phylogen...
Article
Full-text available
Extensive survey to detect and identify phytoplasmas associated with Chilean grapevines was performed from 2003 to 2005 sam- pling symptomatic or asymptomatic grapevine plants. Nested PCR amplification allow the detection of phytoplasmas in 28 out of 90 samples. RFLP analyses identified phytoplasmas as belonging to ribosomal subgroups 16SrI-B, 16Sr...
Article
During surveys performed from 2001 to 2003 in peach orchards a severe decline of trees was observed. Leaf symptoms start in early summer and in one to two weeks the plants dry up and die. Percentages of affected plants varied according to cultivars and years. To verify phytoplasma presence after exclusion of other pathogens by visual inspections an...
Article
The two viroids known to affect stone fruit trees are Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). Viroids can be detected by biological, biochemical or molecular methods. The two first approaches are time consuming and expensive, with the limiting step for molecular techniques being the sample preparation process. In the present...