Nicholas James Morris

Nicholas James Morris
Newcastle University Medicine Malaysia | NUMED

PhD Glasgow University, UK

About

34
Publications
15,460
Reads
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1,164
Citations
Introduction
Skills and Expertise
Additional affiliations
January 1999 - January 2008
Newcastle University
Position
  • Lecturer
January 1994 - December 1998
Geisel School of Medicine at Dartmouth
Position
  • PostDoc Position
October 1990 - September 1993
University of Glasgow
Position
  • PhD Student
Education
October 1990 - September 1993
University of Glasgow
Field of study
  • Biochemistry

Publications

Publications (34)
Article
Full-text available
We report the whole genome ChIP seq for human TOP2B from MCF7 cells. Using three different peak calling methods, regions of binding were identified in the presence or absence of the nuclear hormone estradiol, as TOP2B has been reported to play a role in ligand-induced transcription. TOP2B peaks were found across the whole genome, 50% of the peaks f...
Article
Full-text available
We provide here a detailed and comprehensive analysis of skeletal muscle metabolomic profiles in response to adiponectin in adiponectin knockout (AdKO) mice after high-fat diet (HFD) feeding. Hyperinsulinemic-euglycemic clamp studies showed that adiponectin administration corrected HFD-induced defects in post/basal insulin stimulated R(d) and insul...
Article
Full-text available
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires removal of topoisomerase II. To analyse removal of topoisomerase II from genomic DNA we adapted the...
Article
Full-text available
Tumor cells very often have elevated expression of HSP70, the anti-apoptotic properties of which contribute to overall tumor survival. Independent of its anti-apoptotic properties, HSP70 was also suggested to be involved in the antigen presentation process by chaperoning cytosolic peptides, thus protecting them from rapid degradation and securing t...
Article
Fabrication of truly flexible optoelectronic devices, such as flexible displays and flexible photovoltaics, is highly dependent on the mechanical integrity of individual thin inorganic/organic hybrid device components. A common feature of almost all thin composite film components for flexible optoelectronic applications is indium tin oxide (ITO) co...
Article
Full-text available
The seed proteome of a low phytic acid (lpa) rice line (Os-lpa-XS110-1), developed as a novel food source, was compared to that of its parental line, Xiushui 110 (XS-110). Analysis by surfaced enhanced laser desorption ionization-time-of-flight mass spectrometry (SELDI-TOF MS) and two-dimensional gel electrophoresis (2-DE) allowed the detection of...
Article
Full-text available
Heat shock protein 70 (HSPA) is a molecular chaperone which has been suggested to shuttle human leukocyte antigen (HLA) epitope precursors from the proteasome to the transporter associated with antigen processing. Despite the reported observations that peptides chaperoned by HSPA are an effective source of antigens for cross-priming, little is know...
Article
Full-text available
The gel-forming properties of mucus are closely related to its functioning; although there is limited information available relating to coral mucus gels. The present study investigates coral mucus glycoprotein using rheological methods. We demonstrate the presence of a high-molecular-weight polymeric glycoprotein similar to that found in vertebrate...
Article
Full-text available
A method previously used to measure thickness of the surface mucus layer (SML) of the mammalian gastrointestinal tract has been applied to the SML of reef corals. It involves manual measurement of mucus thickness using a micromanipulator and fine glass needle (micropipette) and is non-destructive to the coral, meaning that repeated measurements can...
Article
Full-text available
the MIAPE Gel Electrophoresis (MIAPE-GE) guidelines specify the minimum information that should be provided when reporting the use of n-dimensional gel electrophoresis in a proteomics experiment. Developed through a joint effort between the gel-based analysis working group of the Human Proteome Organisation's Proteomics Standards Initiative (HUPO-P...
Article
During flexible optoelectronic device packaging, acid containing layers initiate the functional failure of the underlying indium tin oxide film. We experimentally investigate the stressassisted corrosion cracking of thin conductive optoelectronic components. It is essential to understand the stress corrosion mechanisms in order to design highly dur...
Article
We have used primary human muscle cell cultures to investigate the role of glycogen loading in cellular insulin resistance. Insulin pre-treatment for 2 h markedly impaired insulin signaling, as assessed by protein kinase B (PKB) phosphorylation. In contrast, insulin-dependent glycogen synthesis, glycogen synthase (GS) activation, and GS sites 3 de-...
Article
The bioscience field has seen some spectacular advances in genomic and proteomic technologies that are able to deliver vast quantities of information on cellular activity. Such technologies are of critical importance to biology, medical science and in drug discovery. However, living systems are highly complex and to fully exploit these technologies...
Article
Full-text available
Insulin stimulates the movement of glucose transporter-4 (Glut4)-containing vesicles to the plasma membrane of adipose cells. We investigated the role of post-Golgi t-soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) in the trafficking of Glut4 in 3T3-L1 adipocytes. Greater than 85% of syntaxin 6 was found in Glut4-con...
Article
In the course of characterizing proteins present in a preparation of vesicles from rat adipocytes containing glucose transporters, we examined a protein that migrated at 115 kDa upon SDS gel electrophoresis (designated vp115). Sequences of tryptic peptides were obtained, and from this information the cDNA for rat vp115 was cloned. The cDNA encodes...
Article
In the course of our examination of proteins associated with the GLUT4-containing vesicles of rat adipocytes we have identified a new 22 kDa member of the family of endoplasmic reticulum (ER) proteins known as reticulons. The protein, which we refer to as vp20, was purified from a preparation of GLUT4-containing vesicles of rat adipocytes, and tryp...
Article
Vesicles containing the glucose transporter GLUT4 from rat adipocytes contain a major protein of 110 kDa. We have isolated this protein, obtained the sequences of peptides, and cloned a large portion of its cDNA. This revealed that the protein is sortilin, a novel membrane protein that was cloned in another context from a human source while this wo...
Article
We previously discovered that insulin stimulates the marked translocation of a novel membrane aminopeptidase, designated vp165 for vesicle protein of 165 kDa, to the cell surface in adipocytes. To examine the hypothesis that this enzyme acts on peptide hormones, we assessed the relative affinity of the enzyme for 22 peptide hormones by measuring th...
Article
A 97-kDa protein present in the glucose transporter (GLUT4 isotype)-containing vesicles from rat adipocytes has been isolated, the sequences of two tryptic peptides were obtained, and on the basis of these its cDNA partially cloned. The 97-kDa protein is almost certainly identical to a major integral glycoprotein of this size in the rat adipocyte p...
Article
Full-text available
Streptozotocin-induced diabetes caused a profound increase in the steady-state level of phosphorylation of the alpha-subunit of the adenylate cyclase inhibitory protein Gi2 in hepatocytes. Unlike hepatocytes from control animals, those from streptozotocin-diabetic animals showed no increase in the phosphorylation of Gi2 alpha in response to a chall...
Article
Full-text available
A novel membrane aminopeptidase has been identified as a major protein in vesicles from rat adipocytes containing the glucose transporter isotype Glut4. In this study we have characterized this aminopeptidase, referred to as vp165, in 3T3-L1 adipocytes. The subcellular distributions of vp165 and Glut4 were determined by immunoisolation of vesicles...
Article
Full-text available
Challenge of intact hepatocytes with insulin reduced the level of phosphorylated alpha-Gi-2 found under basal (resting) conditions. At maximally effective concentrations of insulin the steady-state labelling of alpha-Gi-2 was reduced by approximately 21%. Insulin achieved this in a time- and dose-dependent fashion, exhibiting an IC50 value of 109 +...
Article
Full-text available
A phosphorylated form of alpha-Gi-2 (the alpha-subunit of Gi-2), immunoprecipitated from hepatocytes under basal conditions, migrated as a single species of pI approximately 5.7, the labelling of which increased approximately 2-fold in cells challenged with either vasopressin or phorbol 12-myristate 13-acetate (PMA); agents which activate protein k...
Article
Challenge of intact hepatocytes with amylin only succeeded in elevating intracellular cyclic AMP levels and activating phosphorylase in the presence of the cAMP phosphodiesterase inhibitor IBMX. Both amylin and CGRP similarly activated adenylate cyclase, around 5-fold, although approximately 400-fold higher levels of amylin were required to elicit...
Article
Full-text available
Both amylin and calcitonin-gene-related neuropeptide (CGRP) activated adenylate cyclase activity in hepatocyte membranes around 5-fold in a dose-dependent fashion, with EC50 values of 120 +/- 14 and 0.3 +/- 0.14 nM respectively. Whereas amylin exhibited normal activation kinetics (Hill coefficient, h approximately 1), CGRP showed kinetics indicativ...
Article
The localisation and immunochemical identification of 3 different forms of protein kinase C (PKC-alpha, PKC-beta and PKC-gamma) in retinas of different species were analysed by immunohistochemistry and SDS-PAGE-Western blotting, respectively. Only in some cases was there a correlation between the findings from each procedure. One reason for the lac...
Article
Using a monoclonal antibody against protein kinase C (PKC) that recognises the isoenzymes alpha, beta I, and beta II, positive immunoreactivity was observed throughout the cytoplasm of bipolar cells in both rat and rabbit retinas. PKC immunoreactivity was also associated with the outer segment of photoreceptors in the rabbit retina and presumed ama...
Article
Monoclonal antibodies to DARPP-32 recognise an antigen which is present in specific neurones in the snail (Helix aspersa). Consecutive sections 10-microns-thick processed for the localisation of DARPP-32 and tyrosine-hydroxylase immunoreactivity did not show a coexistence in any neuronal structures. DARPP-32 positive cells were, however, often morp...
Article
Full-text available
Background: The numerous methods and techniques employed in proteomics investigations generate vast quantities of heterogeneous data, both results and provenance. A standard representation of proteomics data and techniques is urgently required in order to document a proteomics investigation. A standard representation will facilitate data exchange,...

Questions

Questions (5)
Question
Hi,
Anyone have any information on data export from the HumaReader HS ELISA plate reader?
Thanks
Nick
Question
I am looking for a supplier of C. elegans in Malaysia.
If you know of any company that can supply the organism (I am only looking for a couple of plates for undergraduate practicals) I would be grateful if you could share the information.
Thanks
Question
As part of setting up a new undergraduate lab I have been offered a CBS Scientific DCX-700 SDS-PAGE and Western Blotting system. However, I can't understand how it works for blotting, and I can't get my hands on a demo unit. (The reason I am interested is it appears to be one of the few systems that can use pre-poured gels from a range of suppliers, and also pour-your-own.)
The system uses one tank in which SDS-PAGE, and blots can be run (obviously not at the same time). The bit I am struggling with is the blotting as it appears to use the same electrode configuration as for the SDS-PAGE, that is an electrode at the top of the tank and one at the bottom. From my own many years of experience of Western blotting I know that you need a constant uniform field at 90 degrees to the gel to move the proteins to the membrane. However, the approach this system uses would appear to give a non-uniform field that is not at 90 degrees.
According to the company the system 'utilizes a "single electrode array" for electroblotting transfers', and they also seem to acknowledge the non-uniform nature of the field as they recommend loading the gel upside down (i.e. high MW at the bottom of the tank) to take advantage of the better field near the electrode at the bottom of the tank. This all strikes me as a bit odd.
Anyone used a DCX-700 or have any views or opinions, or suggestions?
Question
I am in the process of setting up a new undergraduate lab and I am looking at CO2 cell culture incubators.
Several companies have offered copper-lined incubators as they claim it reduces culture infections. However, having read around I am not convinced. Some researchers think copper-lined incubators are great, others think they are a gimmick.
Has anyone on here used copper-lined incubators? Are they any good? Do they work? Or is it a gimmick?
Question
I am looking for a good all round gel and western blot documentation system for a teaching lab. The system should be able to capture coomassie and silver stained SDS-PAGE gels, DNA (agarose) gels stained with one of the modern replacements for ethidium bromide, and western blots both ECL fluorescent type and old fashioned colorimetric stained blots. That is, it should be able to UV and white light transmission, fluorescence, and reflected light capture. Gel/blot size around 10 x 10 cm up.
I have been recommended the following by suppliers:
Cleaver Scientific - microDoc
Vilber Lourmat - E-BOX VX2
Cole-Parmer - Bio-Doc-IT
Biorad - GelDoc XR+
Aplegen - Omega Lum G
GE Healthcare - IMAGEQUANT LAS500
Does any one here have any thought or opinions on these?

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