Nguyen QuiHue University · Institute of Biotechnology
Nguyen Qui
Doctor of Philosophy
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8
Publications
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Citations since 2017
Publications
Publications (8)
This study aimed to investigate β-casein gene polymorphisms in Jersey cows in Japan. Blood samples were collected from 590 cows from eight Jersey farms in Okayama Prefecture, western Japan. Sequence analysis of exon 7 regions in chromosome 6 of the CSN2 gene revealed the genotype and allele frequencies of the β-casein variants. Considering that var...
Immunoglobulin A (IgA) is a major antibody in the gut. We previously observed that high-fat diet (HFD) feeding reduces IgA reactivity to gut microbiota, but the physiological implications have yet to be elucidated. We hypothesized that a reduction of IgA reactivity to gut microbiota induced by HFD may contribute to development of gut dysbiosis and...
Microbiota of individual cow milk, bulk tank milk, and feces of Jersey cows were examined. Samples were collected from two farms (F1 and F2) in cool (November, Nov) and hot (July, Jul) seasons. Milk yield and milk composition were similar between the two farms and between the two seasons. Prevalent taxa of the fecal microbiota, i.e. Ruminococcaceae...
p> Abstract: The present study clarifies the effect of locally isolated Eimeria species on chickens in central Vietnam. Oocysts of Eimeria species were isolated from feces suspected to be infected with coccidiosis in 3 farms in Huong Thuy district, Thua Thien Hue province. A total of 54 2-week-old chickens were randomly allocated to 2 groups: 3 rep...
Microbiota of the gut, milk, and cowshed environment were examined at two dairy farms managed by automatic milking systems (AMS). Feed, rumen fluid, feces, milk, bedding, water, and airborne dust were collected and the microbiota on each was assessed by Illumina MiSeq sequencing. The most abundant taxa in feed, rumen fluid, feces, bedding, and wate...
Tóm tắt: Đặc điểm sinh lý và năng suất sinh sản của lợn nái lai Landrace × (Pietrain × VCN-MS15) và Landrace × (Duroc × VCN-MS15) nuôi tại Thừa Thiên Huế đã được đánh giá trong nghiên cứu này. Tổng số 16 cá thể lợn cái hậu bị Landrace × (Duroc × VCN-MS15) và Landrace × (Pietrain × VCN-MS15) được nuôi theo nhóm (2 nhóm/tổ hợp và 4 cá thể/nhóm) trong...
Taro leaves (Colcacia esculenta) were ensiled alone or with equal parts of taro petioles or 5% molasses. After 21 days, the lactic acid concentrations (% in DM) were 1.27, 1.45 and 1.49, and pH was 4.5, 4.0 and 3.9, for leaves alone, leaves plus petioles and leaves plus molasses, respectively. Ensiling the taro leaves reduced oxalate concentrations...
The objectives of the present study were to evaluate the effect on nutritive value of a diet for growing pigs of silage with increasing proportions (from 0 to 60% as DM) of banana pseudo-stem eplacing taro foliage. Compared with the tara foliage (leaves plus petioles), the banana pseudo-stem had half the crude protein, twice as much NDF and negligi...
Questions
Questions (5)
I'm extracting bacterial DNA from milk samples using DNeasy Blood & Tissue kit. However the columns have been used up and I want to complete the DNA extraction as soon as possible. So can I use spin columns from QIAamp DNA Stool Mini kit instead of that from DNeasy Blood & Tissue kit? In other word, is there any differences between QIAamp DNA Stool Mini spin column and DNeasy Blood & Tissue spin column? Both of them are from QIAGEN company.
Thank you!
I have three types of samples including liquid samples (water, milk), solid samples (feed, feces) and aerosol samples. After quantifying by qPCR, I have the copies number of each sample. However, I don't know how to compare the number of bacteria among these samples because of the differences in their characteristic and difference in unit (g vs ml) as well. Could anyone give me suggestion? Many thanks.
I'm doing experiment on LAB, specifically, lactobacillus plantarum. In my experiment, I inoculated l. plantarum in substrate-containing serum bottles ( in vitro gas production technique) and checked the total gas production, total SCFA concentration and do qPCR to check their initial quantities. The results showed that there was no difference between control and treatments. It means that l. plantarum couldn't survive in the buffered rumen fluid. Is it due to the neutral pH of the rumen? Could anyone explain this for me? Thanks in advance.
I'm going to clone bacterial DNA after doing DGGE. I wonder that why DNA cloning is necessary meanwhile PCR can amplify these DNA fragments. Why don't we use directly these PCR products to sequence?
Thanks in advance.
I saw many papers calculating the Acetic : Propionic acid ratio when they do experiment about ruminal fermentation, both in vitro and in vivo. But I don't fully understand about its meaning. Could you please tell me? Thank you in advance.
