Ngoc Bich Vu

• Associate Professor, Ph.D
• Head of Department at Ho Chi Minh City University of Science

Introduction: Photoaging is a degenerative condition that leads to skin fragility, loss of function and cosmetic dissatisfaction. The senescence of human dermal fibroblasts (HDFs) plays vital roles in the pathogenesis of skin aging. Therefore, we aim to develop a senescent HDFs model with an optimized acute ultraviolet (UV) irradiation protocol for...

Introduction: The viability of mesenchymal stem cells (MSCs) during intravenous infusion can significantly impact treatment efficacy, particularly with thawed cryopreserved MSCs. During infusion, these thawed MSCs are suspended in saline at room temperature, which lacks nutrients and buffering capacity, leading to a gradual decline in cell viabilit...

Mở đầu: Tế bào gốc trung mô từ mô mỡ (ADSC) là tế bào gốc trưởng thành, dễ thu thập từ người trưởng thành. Nhiều nghiên cứu đã chứng minh tế bào gốc trung mô từ mô mỡ có khả năng điều hoà miễn dịch, nên có hiệu quả trong các bệnh lý về bất thường miễn dịch. Nghiên cứu của chúng tôi đánh giá hiệu quả điều trị của ADSC dùng đường tiêm tĩnh mạch trong...

Introduction: Mesenchymal stem cells (MSCs) are increasingly used for the treatment of various diseases. However, the treatment cost remains relatively high. One reason for this is the complexity and expense involved in the transportation and use of MSCs. Currently, most of the transport of stem cells from the production site to the hospital is car...

Platelet-rich plasma (PRP) therapy has gained popularity as a treatment option for osteoarthritis, despite a lack of formal endorsement by professional associations. While not universally recommended, its application in managing joint osteoarthritis and aiding ligament regeneration has received approval in several countries. Research into PRP's eff...

Introduction: Psoriasis is a chronic inflammatory skin disorder with a complex pathogenesis, prominently involving the IL-17/IL-23 pathway. The imiquimod (IMQ)-induced mouse model is widely utilized for preclinical psoriasis research. This study aims to elucidate the effects of different durations of IMQ exposure on the development and characterist...

Introduction: Deer antlers are remarkable organs as they can regenerate seasonally and leave no scars. Antler-derived stem cell therapy applications are of increasing interest in food, beauty, and medicine. Method: Antler stem cells (ASCs) were isolated from antlers, and their expression of markers CD73, CD90, CD105, Nanog, and Oct4 was detected by...

Regenerative medicine-based therapies are considered promising for some chronic diseases, such as osteoarthritis (OA). Because OA is the most common disease in many countries, significant efforts have long been made to develop effective treatments. Current therapies for OA include hyaluronic acid, platelet-rich plasma, bone marrow aspirate concentr...

Introduction: Exosomes derived from mesenchymal stem cells (MSCs) are crucial mediators of the paracrine effects as well as tissue repair and have promising clinical applications. They enhance tissue regeneration by reducing inflammatory responses, enhancing proliferation, inhibiting apoptosis, and stimulating angiogenesis. This study aimed to eva...

Objectives: Acute hindlimb ischemia is a peripheral arterial disease that severely affects the patient's health. Injection of stem cells-derived exosomes that promote angiogenesis is a promising therapeutic strategy to increase perfusion and repair ischemic tissues. This study aimed to evaluate the efficacy of adipose stem cell-derived exosomes in...

Introduction: Recent studies have demonstrated that adipose tissue-derived stem cell (ADSC) transplantation could promote neoangiogenesis in various ischemic diseases. However, as whole cells, ADSCs have some disadvantages, such as shipping and storage issues, high costs, and controversies related to the fates of grafted cells in the recipients. T...

The skin is an organ that performs complex functions of both the innate and adaptive immune systems. It serves as the first physical barrier to protect the body from environmental factors. Skin also carries aesthetic value in people's desire for eternal youth. Skin lesions are often unwanted, causing open wounds that can be contagious or permit inf...

Introduction: In regenerative medicine, human adipose tissue–derived mesenchymal stem cells (hadMSCs) have exhibited tremendous promise as an efficient approach to chronic cardiovascular diseases. Noble effects of mesenchymal stem cells (MSCs) have been acknowledged to contribute to not only cellular engraftment and response to the site of injury...

Coronavirus disease 2019 (COVID-19) has caused nearly 15 million deaths worldwide. The rapid development of COVID-19 vaccines and anti-viral drugs significantly decreased the level of mortality related to COVID-19. However, post-COVID-19 pulmonary fibrosis has become a severe problem for some COVID-19 patients. The previous articles present the res...

Introduction: In recent years, both stromal vascular fraction (SVF) from adipose tissue and mesenchymal stem cells (MSC) from adipose tissues were extensively used in both preclinical and clinical treatment for various diseases. Some studies reported differences in treatment efficacy between SVFs and MSCs in animals as well as in humans. Therefore...

Introduction: Cartilage and bone tissues are widely utilized in regenerative medicine, especially in treating injured cartilage and bone. Some attempts were made to produce these tissues from stem cells and scaffolds for a long time, combined with some growth factors and inducible factors. However, owing to the complexity of cartilage and bone tiss...

Introduction: The damaged articular cartilage has limited self-regeneration capacity because of the absence of blood vessels, lymphatics, and nerves. Cartilage transplantation is, hence, a popular method used to treat this disease. However, sources of autograft and allogenic cartilage for transplantation are limited. Therefore, this study aims to...

Introduction: Articular cartilage is limited in self-repair following injuries due to avascular, lymphatic, and nerve absence. Recent treatments for cartilage injuries, such as physical therapy, anti-inflammatory medication, chondrocyte implantation, and joint replacement, still have limitations. This study aimed to evaluate the treatment efficacy...

Introduction: After injuries, the cartilage healing capacity is limited owing to its nature as a particular connective tissue without blood vessels, lymphatics, or nerves. The creation of artificial cartilage tissue mimics the biological properties of native cartilage and can reduce the need for donated tissue. Fibrin is a type of biodegradable sc...

BACKGROUND: Angiogenesis plays an important role in tissue repair. In recent years, one of the main research strategies used to induce angiogenesis in vivo has been via stem cell therapy, especially therapy using mesenchymal stem cells (MSCs). However, the use of MSCs in angiogenesis has also been limited due to the low angiogenic potency of MSCs....

Introduction: Mesenchymal stem cell (MSC) transplantation has been reported as a promising therapy for acute limb ischemia (ALI). However, the treatment efficacy is limited to only certain improvements. Therefore, this study aims to improve the treatment efficacy of MSC transplantation through the use of MSC sheets produced from MSCs' cultured in f...

Introduction: The potency of mesenchymal stem cells (MSCs) related to their biological effects includes immune modulation and angiogenesis. Recently, some stress conditions were applied to enhance the angiogenic potential of MSCs. This study aimed to assess the effects of conditioned media (CM) collected from adipose-derived stem cells’ (ADSCs’) cu...

Ischemic diseases are conditions associated with the restriction or blockage of blood supply to specific tissues. These conditions can cause moderate to severe complications in patients, and can lead to permanent disabilities. Since they are blood vessel-related diseases, ischemic diseases are usually treated with endothelial cells or endothelial p...

Introduction: Tissue engineering is a field suited for applying stem cells, besides stem cell transplantation. In the current tissue engineering approaches, stem cells are typically seeded onto a suitable scaffold and induced into specific tissues under particular conditions. However, this strategy has faced some limitations, namely that stem cell...

Wound healing, especially of chronic wounds, is still an unmet therapeutic area since assessment and management are extremely complicated. Although many efforts have been made to treat wounds, all strategies have achieved limited results for chronic wounds. Stem cell-based therapy is considered a promising approach for complex wounds such as those...

This study suggested a new method to produce the in vitro cartilage tissues by cartilage differentiation in spheroids from adipose-derived stem cells on porous scaffolds. Adipose-derived stem cells (ADSCs) were used to produce spheroids by the hanging-drop method. The spheroids were then loaded into a porous scaffold and induced to differentiate in...

Coronavirus disease-2019 (COVID-19) has affected more than 200 countries worldwide. This disease has hugely affected healthcare systems as well as the economy to an extent never seen before. To date, COVID-19 infection has led to about 165000 deaths in 150 countries. At present, there is no specific drug or efficient treatment for this disease. In...

Tế bào gốc trung mô từ mô mỡ (Adipose Tissue-Derived Stem Cells-ADSCs) có những ưu điểm như khả năng tự làm mới, biệt hóa thành nhiều loại tế bào, dễ dàng thu nhận, ít gây xâm lấn, số lượng tế bào dồi dào và đặc biệt là có thể sử dụng làm nguồn tự ghép. Để thực hiện các nghiên cứu nhằm ứng dụng ADSCs cho y học tái tạo và công nghệ mô, việc chuyển g...

Introduction: Cartilage damage is one of the injuries that is difficult for the human body to self-repair due to the avascular and completely mature tissue with only few stem or progenitor cells present. Recently, some studies showed that engineered cartilage tissues could be used to treat or improve this injury. This study aimed to produce the car...

Introduction: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide. COPD results from chronic inflammation of the lungs. Current treatments, including physical and chemical therapies, provide limited results. Stem cells, particularly mesenchymal stem cells (MSCs), are used to treat COPD. Here, we evaluated the...

Autoimmune diseases (ADs) are common conditions of human health. These diseases can significantly reduce the patients’ quality of life. Although most autoimmune diseases can be controlled by certain immunosuppressive drugs, after long-term treatment, the therapeutic efficacy of these drugs can be significantly decreased while side effects may be in...

Introduction: Human umbilical cord-derived mesenchymal stem cells (UCMSCs) are multiple potential stem cells that can differentiate into various kinds of functional cells, including adipocytes, osteoblasts, and chondroblasts. Thus, UCMSCs have recently been used in both stem cell therapy and tissue engineering applications to produce various functi...

Introduction: Human mesenchymal stem cells (MSCs) are the most popular stem cells applied in disease treatment. MSCs can be isolated and in vitro expanded from various sources such as bone marrow, peripheral blood, umbilical cord blood, umbilical cord tissue, and adipose tissue. According to Dominici et al. (2006), MSCs should express CD105, an ess...

Introduction: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marke...

Introduction: Cartilage injury is the most common injury among orthopedic diseases. The predominant treatment for this condition is cartilage transplantation. Therefore, production of cartilage for treatment is an important strategy in regenerative medicine of cartilage to provide surgeons with an additional option for treatment of cartilage defect...

Stem cell research and therapy are one of the most attractive studies in the biomedicine. Not only in the bench, nowadays stem cells but also become the bustling industry. In Vietnam, biomedical scientists started to study and apply stem cells since 1995. From that, Vietnamese scientists got some significant achievements in stem cell research and t...

Exosomes as well as other extracellular vesicles (EVs) play an important role in cell–cell communication. Recently, EVs have been used as a platform for cellular therapy for conditions such as degenerative diseases. EVs were demonstrated to stimulate angiogenesis, cell proliferation, and immune modulation. This study aims to determine the factors,...

Of all stem cells in the human body, mesenchymal stem cells (MSCs) are the most popular with regard to their potential and use in therapy. They can be easily identified and isolated from various tissues, and they can differentiate into a variety of mature cell types (bone, fat, cartilage, etc.). Unlike other adult stem cells, MSCs possess a variety...

Stem cell transplantation has become the new therapy for various degenerative diseases (such as Parkinson’s disease and spinal cord injury), and even for myocardial infarction, type II diabetes mellitus, and cancer. From the first transplantation of therapeutic stem cells in humans to the present day, the use of stem cells or stem cell-based produc...

Mesenchymal stem cells (MSCs) are common stem cells in the human body. They have various properties and functions, including migration to tumors. Based on this property, MSCs have been used as vectors to deliver anti-cancer agents to tumors. To date, MSCs have been modified to express some factors or agents, including interleukin (IL)-12, IL-14, IF...

According to World Health Organization, cardiovascular disease, especially ischemic heart disease, is one of the top ten killers today. Therefore, a growing number of new therapies are being studied for curing this disease from new drug developments to new advance stent creations. Although these methods have given patients more comfortable than pre...

Introduction: Pathological animal models provide the foundation for developing new methods for disease treatment. This research aims to establish a rabbit model of femoral head necrosis. Methods: Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylprednisolone (MPS) combined with Complete Freund's Adjuvant (CFA). New Zea...

Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and ne...

Currently, Adipose-derived Stem Cells (hASCs) therapy is being promoted applied research in the treatment of many diseases, especially autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis and type 1 diabetes. However, profound mechanisms of this potential therapy have not been understood entirely. It is questioned that whi...

Endothelial progenitor cells (EPCs) play an important role in angiogenesis. However, they exist in limited numbers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F12 m...

Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and ne...

Background: CD47 is a transmembrane glycoprotein expressed on all cells in the body and particularly overexpressed on cancer cells and cancer stem cells of both hematologic and solid malignancies. In the immune system, CD47 acts as a "don't eat me" signal, inhibiting phagocytosis by macrophages by interaction with signal regulatory protein α (SIRPα...

Nowaday, the regenerative medicine based on the use of biomaterial scaffold combine to stem cells was considered as a necessary treatment strategy for injury or diseases involving cartilage. In this study, we investigated the adherence, proliferation and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (rBM-MSCs) on...

Introduction: Type 2 diabetes mellitus (T2D) is the most common form of diabetes mellitus, accounting for 90% of diabetes mellitus in patients. At the present time, although T2D can be treated by various drugs and therapies using insulin replacement, reports have shown that complications including microvascular, macrovascular complications and ther...

Background: Pathological animal models provide the foundation for developing new methods for treating. This research aims to establish a rabbit model of femoral head necrosis. Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylpresnisolone combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were div...

Ischemia is the reduction of blood flow to tissues by injury of blood vessels. Depending on the sites of tissues and grade of ischemia, ischemia can cause many serious complications. This study aimed to evaluate the effects of the E-twenty six (ETS) factor Ets variant 2 (ETV2) gene expression in angiogenesis and the effect of ETV2 gene therapy in a...

The cell migration and/or molecular mechanism in healing of damaged vascular or muscle tissue are emerging field with strong research interest worldwide. This study focuses on evaluating the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) in restoring damaged tissue through the migration of cells and the expression of genes relate...

Introduction: Both ETV-2 and HGF factors are known to be important factors which trigger neo-angiogenesis both in vitro and in vivo models. This study aimed to treat hindlimb ischemia in mice by co-injection of ETV-2 and HGF viral vectors. Methods: ETV-2 and HGF vectors were prepared per previous publications. The mouse ischemic hindlimb model was...

Cell migration and molecular mechanisms during healing of damaged vascular or muscle tissues are emerging fields of interest worldwide. The study herein focuses on evaluating the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) in restoring damaged tissues. Using a hindlimb ischemic mouse model, ADMSC-mediated induction of cell mig...

The Figure 5 of the article “Adipose derived stem cell transplantation is better than bone marrow mesenchymal stem cell transplantation in treating hindlimb ischemia in mice” that published in 29 Sept 2016, at Vol 3, Issue 9, from page 844-856 (DOI: 10.7603/s40730-016-0046-0) as below that lacked the legend for the violet line should be corrected...

The title of the article “A mouse model of osteonecrotic femoral head induced by methylprednisolone and liposaccharide” published in 25 March 2016, in 2016 at Vol 3, Issue 3, from pages: 548-556 (DOI: 10.7603/s40730-016-0012-x) should be read as: “A mouse model of osteonecrotic femoral head induced by methylprednisolone and lipopolysaccharide”.

Ischemia is the reduction of blood flow to tissues by injury of blood vessels. Depending on the sites of tissues and grade of ischemia, ischemia can cause many serious complications. This study aimed to evaluate the effects of the E-twenty six (ETS) factor Ets variant 2 (ETV2) gene expression in angiogenesis and the effect of ETV2 gene therapy in a...

In vitro production of tissues or tissue engineering is a promising approach to produce artificial tissues for regenerative medicine. There are at least three important components of tissue engineering, including stem cells, scaffolds and growth factors. This study aimed to produce cartilage tissues in vitro from culture and chondrogenic differenti...

Introduction: Type 2 diabetes mellitus (T2D) is the most common form of diabetes mellitus, accounting for 90% of diabetes mellitus in patients. At the present time, althoughT2D can be treated by various drugs and therapies using insulin replacement, reports have shown that complications including microvascular, macrovascular complications and thera...

Introduction: Chronic obstructive pulmonary disease (COPD) is a chronic disease affecting the airway of the respiratory system. COPD cases have rapidly increased in recent years, with the disease becoming the fourth leading cause of death worldwide. Stem cell transplantation is a new approach to treat COPD. In this study we report in two cases the...

Endothelial progenitor cells (EPCs) play an impor- tant role in angiogenesis. However, they exist in limited num- bers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F...

Induced pluripotent stem cells (iPSCs) are a novel source of stem cells with great potential for therapeutic applications. By transfection of pluripotent genes, iPSCs can be obtained from almost all adult somatic cells. This technique allows for easy production of large amounts of iPSCs for autografts or allografts. However, to use these cells in t...

Recent clinical applications of stem cells have demonstrated novel therapies for chronic diseases and certain kinds of cancers. The first clinical case was treated with hematopoietic stem cells over 50 years ago. Subsequently, mesenchymal stem cells (MSCs) have been clinically applied to various diseases. Furthermore, induced pluripotent stem cells...

Background: CD47 is a transmembrane glycoprotein expressed on all cells in the body and particularly overexpressed on cancer cells and cancer stem cells of both hematologic and solid malignancies. In the immune system, CD47 acts as a " don't eat me " signal, inhibiting phagocytosis by macrophages by interaction with signal regulatory protein α (SIR...

Introduction: Human fibroblasts can be differentiated into endothelial progenitor cells by direct reprogramming via ETV-2 transfection. Previously, we have shown that the efficacy of direct reprogramming can be enhanced by hypoxia treatment. In this study, we aim to investigate whether the efficacy of direct reprogramming of fibroblasts into EPCs v...

Introduction: Bone marrow derived MSCs (BM-MSCs) and adipose derived MSCs (AD-MSCs) are among the types of stem cells most commonly studied. Our study aims to compare the therapeutic efficacy of allograft AD-MSCs versus BM-MSCs in a mouse model of hindlimb ischemia. Methods: AD-MSCs were isolated from belly fat and BM-MSCs were isolated from femur...

Introduction: Mesenchymal stem cells (MSCs) transplantation for the treatment of acute hindlimb ischemia is recently attracting the attention of many scientists. Identifying the role of donor cells in the host is a crucial factor for improving the efficiency of treatment. This study evaluated the injury repair role of xenogeneic adipose-derived ste...

Significance: Arthroscopic microfracture (AM) and stem cell therapy have been used clinically to treat osteoarthritis (OA). This study evaluated the clinical effects of AM in the presence (treatment group) and absence (placebo group) of a stromal vascular fraction (SVF) injection in the knee for OA. The SVF was suspended in platelet-rich plasma (P...

Background Endothelial progenitor cell (EPC) transplantation is a promising therapy for ischemic diseases such as ischemic myocardial infarction and hindlimb ischemia. However, limitation of EPC sources remains a major obstacle. Direct reprogramming has become a powerful tool to produce EPCs from fibroblasts. Some recent efforts successfully direct...

Introduction: Mesenchymal stem cells (MSCs) show great promise in regenerative medicine. Clinical applications of MSCs have recently increased significantly, especially for immune diseases. Autologous transplantation is considered a safe therapy. However, its main disadvantages are poor stability and quality of MSCs from patient to patient, and lab...

Mesenchymal stem cells (MSCs) now are popular stem cells for clinical applications. To date, MSCs were accepted in various disease treatments with several FDA-approved treatments in some countries. One important requirement for the clinical usage of stem cells is the production of stem cells. Actually, the treatment efficacy of MSC transplantation...

Mesenchymal stem cells (MSCs) now are popular stem cells for clinical applications. To date, MSCs were accepted in various disease treatments with several FDA approved treatments in some countries. One important requirement for the clinical usage of stem cells is the production of stem cells. Actually, the treatment efficacy of MSC transplantation...

Umbilical cord (UC) is a rich source of rapidly proliferating mesenchymal stem cells (MSCs) that are easily cultured on a large-scale. Clinical applications of UC–MSCs include graft-versus-host disease, and diabetes mellitus types 1 and 2. UC–MSCs should be isolated and proliferated according to good manufacturing practice (GMP) with animal compone...

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase us...

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase us...

Introductions: Joint replacements have considerably improved the quality of life of patients with damaged joints. Over the past 30 years, there has been much effort and investigations in ways to repair damages in joints, including knee and hip joints. Materials for joint production have also been developed. Many improvements have been made in the j...

Introduction: Osteonecrosis of the femoral head is caused by various factors, including prolonged use of steroid drugs, use of alcohol, vascular injuries and hemoglobinopathies. This study aims to develop a mouse model for glucocorticoid-induced avascular necrosis (AVN) of the femoral head.Methods: Adult mice were randomly divided into two groups:...

Adipose-derived stem cells (ADSCs) are a promising mesenchymal stem cell source with therapeutic applications. Recent studies have shown that ADSCs could be expanded in vitro without phenotype changes. This study aimed to evaluate the effect of hypoxia on ADSC proliferation in vitro and to determine the role of vascular endothelial growth factor (V...

Avascular necrosis of the femoral head (AVNFH) is a condition involving low blood supply to the femoral head. Lacking blood, the femoral head degenerates. Although there are certain techniques to treat this disease, the treatment efficiency does not significantly improve the symptoms, especially in the late stage of AVNFH. Currently, AVNFH is treat...

Because of self-renewal, strong proliferation in vitro , abundant sources for isolation, and a high differentiation capacity, mesenchymal stem cells are suggested to be potentially therapeutic for liver fibrosis/cirrhosis. In this study, we evaluated the treatment effects of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) on mouse liver...

Gingival stem cells (GSCs) are a novel source of mesenchymal stem cells (MSCs) that are easily accessed from the oral cavity. GSCs were considered valuable autograft MSCs with particular characteristics. However, the limitation in the number of available GSCs remains an obstacle. Therefore, this study aimed to stimulate GSC proliferation by ascorbi...

The adipose tissue contains a large numbers of stem cells; adipose-derived stem cells (ADSCs) can be employed in regenerative medicine. This study was aimed at isolating ADSCs and evaluating the safety of ADSCs in mouse models. Stromal vascular fraction (SVF) was collected from the adipose tissue using collagenase. ADSCs were then isolated from SVF...

Non-expanded adipose tissue-derived stem cells (ADSCs) are commonly used in preclinical and clinical articular cartilage injury treatment; however, there are usually insufficient non-expanded ADSCs for transplantation. This research aims to evaluate the safety and efficacy of expanded ADSC transplantation in a mouse model. The stromal vascular frac...

Limb ischemia is a common disease that occurs when there is a sudden lack of blood flow to a limb due to embolism or thrombosis. Currently, treatment of this disease is extremely difficult and shows poor efficacy. De novo angiogenesis based on stem cell therapy is considered as a promising therapy for treating this disease. Therefore, this study ai...

Adipose-derived stem cells (ADSCs) are excellent for regenerative medicine. Like mesenchymal stem cells, ADSCs possess multi-potent differentiation capacity that enables them to differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into other cells. ADSC transplantation has gained attention in recent years, e...

Acute lower limb ischemia is a common peripheral artery disease whose treatment presents many difficulties. Stem cell transplantation is considered a novel and promising method of treating this disease. Umbilical cord blood (UCB) is rich in stem cells, including hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) and endothelial progenit...

Mesenchymal stem cells (MSCs) are an attractive source of stem cells for clinical applications. These cells exhibit a multilineage differentiation potential and strong capacity for immune modulation. Thus, MSCs are widely used in cell therapy, tissue engineering, and immunotherapy. Because of important advantages, umbilical cord blood-derived MSCs...

Introduction: Acute lower limb ischemia is a common peripheral artery disease whose treatment presents many difficulties. Stem cell transplantation is considered a novel and promising method of treating this disease. Umbilical cord blood (UCB) is rich in stem cells, including hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) and endoth...

Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articu...

The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil resistant hepatic cancer cells an...

Breast cancer stem cells with a CD44(+)CD24(-) phenotype are the origin of breast tumors. Strong CD44 expression in this population indicates its important role in maintaining the stem cell phenotype. Previous studies show that CD44 down-regulation causes CD44(+)CD24(-) breast cancer stem cells to differentiate into non-stem cells that are sensitiv...

Human bone marrow derived mesenchymal stem cells (BMMSCs), a source of important autologous stem cells without ethical debate, have been used in both experimental and clinical treatment. Therefore, this research aims to isolate, culture and cryopreserve BMMSCs. Mesenchymal stem cells (MSCs) were preserved in each cryovial by slow freezing – rapid t...

It is known that umbilical cord blood (UCB) is a rich source of stem cells with practical and ethical advantages. Three important types of stem cells which can be harvested from umbilical cord blood and used in disease treatment are hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). Since these s...