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Nevena Zlatković

Nevena Zlatković
University of Belgrade, Faculty of Agriculure

About

17
Publications
2,541
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91
Citations
Citations since 2017
11 Research Items
78 Citations
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201720182019202020212022202305101520
201720182019202020212022202305101520

Publications

Publications (17)
Article
Full-text available
Serious outbreaks of walnut deep bark canker were observed on young walnut trees (Juglans regia L.) in the northern part of Serbia during 2020. From the symptomatic walnut tissues, two types of bacterial colonies were isolated, predominantly, light cream, circular and smooth colonies, as well as small, yellowish, mucoid and convex ones. PCR analysi...
Article
During May 2016 severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. A total of 22 strains were isolated: 16 from symptomatic raspberry shoots, two from asymptomatic raspberry leaves, and four from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks...
Article
Full-text available
Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli , is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management...
Article
Full-text available
Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were ana...
Article
During May 2021, necrosis of young twigs and flower buds were observed on two-year-old highbush blueberry plants (Vaccinium corymbosum) cv. Draper, in a 1 hectare orchard in the municipality of Šabac, Serbia. Disease symptoms included reddish-brown to black irregularly shaped cankers developing on the shoot tips that extended downwards along the br...
Article
Full-text available
In Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial dis...
Article
Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains...
Article
Full-text available
Pseudomonas syringae sensu stricto (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in a...
Article
Full-text available
Three PCR methods, referred in this study as „conventional“, „nested“ and „chromosomal“ PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from differen...
Article
Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of t...
Article
During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease....
Article
Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacte...
Article
Full-text available
Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7T (CFBP 7436T, LMG 26435T), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674),...
Article
Serious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homogeneous biochemical and physiological characterist...

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Projects

Project (1)
Project
Crown gall is an economically important and widespread plant disease caused by tumorigenic agrobacteria, affecting various agricultural crops, primarily fruit species, grapevine and some ornamentals. The disease is caused by different members of the family Rhizobiaceae, distributed within genera Agrobacterium, Allorhizobium and Rhizobium. Nevertheless, it has been shown that crown gall tumors are not only inhabited by agrobacteria. However, previous studies were mainly based on isolation-based methods, which are mainly limited by the fact that large proportion of microorganisms cannot grow on standard nutrient media. Novel tools based on next-generation sequencing make it possible to get an insight into the total bacterial community of desirable ecological niche at relatively low cost. Therefore, the objective of the project is to unravel the diversity of bacteria associated with crown gall tumors on various agricultural crops in Serbia. The understanding of the diversity and role of the crown gall associated microbiota will contribute in development of more efficient management of crown gall disease in the future.