Neslihan Dikbas

Neslihan Dikbas
Ataturk University · Biotechnology Application and Research Center

About

43
Publications
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Publications

Publications (43)
Article
Full-text available
Alternative or complementary control methods need to be developed to reduce the use of hazardous chemicals in agriculture. Biological control of fungi by bacteria is an important alternative. The fungal cell wall consists of chitin, glucan, and glycoproteins. Therefore, bacteria producing hydrolytic enzymes such as chitinases are of great interest...
Article
Full-text available
It has been planned to minimize the yield and quality impairment of the seed corn, which is strategically important in the world, by pests under storage conditions with a biological product produced with a biotechnological approach. In this context, the present study aimed to control the maize weevil Sitophilus zeamais, known as a warehouse pest, u...
Article
In the present study, the phytase enzyme was purified from Lactobacillus plantarum with a 3.08% recovery, 9.57-purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro-micro nutrient content of pansy (Viola x wittrockiana)...
Article
Despite climate change scenarios, new research on improving yield and quality in agricultural production is extremely important. The use of nanomaterials and plant-growth-promoting bacteria (PGPB) has been of interest to researchers in recent years. In the future, the creation of new nano-biotechnological products by using nanomaterials together wi...
Presentation
To discuss agricultural biotechnology application with nanomaterials and Microbial methabolites between academic and ındustriel society.
Article
Aims: Pests are one of the factors that cause problems in plant production. Many methods are used in the fight against economically damaging insects. Mixtures of various substances are used to reduce the negative effects of harmful organisms and to control them. These substances, called pesticides, often have a chemical origin, because they have ma...
Preprint
Full-text available
Background: Phytases can be produced by animals, plants and microorganisms. However, the most promising ones for commercial use and biotechnological applications are those of microbial origin. Phytases are also used in the preparation of myo-inositol phosphates in the food industry, soil remediation and in the paper industry. Biotechnology, along w...
Article
Full-text available
Citrus fruit can be infected by many fungal pathogens, and these pathogens cause considerable losses during storage and transportation. Recently, biological control methods of postharvest diseases are getting more prevalent due to the effects of chemical residues on fruits. The present study was carried out to determine the antifungal activities of...
Article
Full-text available
Phytase (myo-inositol hexaphosphate phosphohydrolase) belongs to phosphatases. It catalyzes the hydrolysis of phytate to less-phosphorylated inorganic phosphates and phytate. Phytase is used primarily for the feeding of simple hermit animals in order to increase the usability of amino acids, minerals, phosphorus and energy. In the present study, ph...
Article
Full-text available
Mannanase alkaline was purified from thermo Lactobacillus plantarum(ATCC® 14917TM) using ammonium sulfate precipitation and DEAE-celluloseion exchange chromatography and Sephacryl S200 Gel filtration chromatography techniques, in 36% yield and 111 -fold. Gel filtration chromatography and SDS-PAGE electrophoresis were executed pure enzyme systems 35...
Article
Objective: Oxidative stress has been reported to be associated with various pregnancy complications and to play key roles in many of them. An inadequate level of antioxidant defense may eventually lead to an early pregnancy loss. There is a lack of information about the roles of the PON2 and PON3 enzymes in the etiology of the cases of unexplained...
Conference Paper
This study was conducted to comparise two identification method for Salmonella spp which includes biochemical test and Microbial Identification System (MIS) methods A total of 120 pieces of chicken samples examined in this study was provided from the markets in Erzurum city. The samples includes 40 pieces of chicken wings, chicken legs and chicken...
Article
In the study, phytase was purified in three simple steps comprising ammonium sulphate precipitation, anion exchange and gel filtration chromatography from Weissella halotolerans. The enzyme was obtained with a specific activity of 227.73 EU/mg and 6.52% recovery. The molecular mass of the enzyme was determined to be 41.52 kDa. The optimum pH and te...
Article
In this study, \(\hbox {TiO}_{2}\) coatings were grown with nano-Ag doped and micro-arc oxidation technique on NiTi shape memory alloy. The antibacterial sensitivity and corrosion resistance characteristics of both coatings were investigated. The microstructure and composition analyses of the coatings were performed with scanning electron microscop...
Article
Thermo alkaline Mannanase, obtained from the Lactobacillus acidophilus (ATCC 4356) bacterium, was purified and as a result 181.3 fold and 36.7% yield were attained. Gel filtration chromatography and SDS PAGE electrophoresis systems are executed to the pure mannanase enzyme, and two subunits of the enzyme were determined at 36 and 73 kDa. The optimu...
Article
Full-text available
he aim of this study was to test for antibacterial efficiacy and potential use as seed disinfectants of extracts and essential oils of Satureja hortensis, Satureja spicigera, Origanum onites and Origanum rotundifolium against Xanthomonas axonopodis pv. vesicatoria causes of bacterial leaf spot of pepper and tomato. According to the in-vitro test re...
Article
Objective: Recent evidence suggests that oxidative stress is involved in the pathophysiology of many human diseases. It has been demonstrated that oxidative stress is associated with intrauterine growth restriction (IUGR), and the depletion of placental antioxidant systems has been suggested as a key factor in this disease. Our aims were to explor...
Conference Paper
Full-text available
The aim of this study is to determine effect of Origanum rotundifolium’s essential oil on some plant pathogenic bacterias, seed germination and plant growth of tomato. Xanthomonas axanopodis pv. vesicatoria strain (Xcv-761) and Clavibacter michiganensis ssp. michiganensis strain (Cmm) inoculated to tomato seed. The seeds were tested for germination...
Article
Full-text available
To determine the role of Bacillus cereus as a potential pathogen in food poisoning, the production of an emetic toxin (cereulide) and enterotoxigenic by B. cereus was isolated in various food sources. Ninety-two strains of B. cereus isolated from milk, cereal, chicken and meat products have been used to study profiles in detail and by polymerase ch...
Article
Full-text available
This study was carried out to determine antibacterial activity of essential oils, extracts and major components of three Artemisia species (A. absinthium, A. santonicum and A. spicigera) against to some seed borne bacterial plant pathogens. According to our results, essential oils and some major components of Artemisia species have antibacterial ac...
Article
Full-text available
Background Chitin is the main structural component of cell walls of fungi, exoskeletons of insects and other arthropods and shells of crustaceans. Chitinase enzyme is capable of degrading chitin, and this enzyme can be used as a biological fungicide against phytopathogenic fungi, as well as an insecticide against insect pests.Methods In this study,...
Article
Full-text available
Phytases (myo-inositol hexakisphosphatephos phohydrolase, EC 3.1.3.8) catalyze the release of phosphate from phytates. Many of the cereal grains, legumes and oilseeds store phosphorus in phytate form. Phytases are used in many areas such as in food industry, in preparation of myo-inositol phosphates, in the paper industry and in soil improvement. I...
Article
Full-text available
Background: Chitin is the main structural component of cell walls of fungi, exoskeletons of insects and other arthropods and shells of crustaceans. Chitinase enzyme is capable of degrading chitin, and this enzyme can be used as a biological fungicide against phytopathogenic fungi, as well as an insecticide against insect pests.
Article
Full-text available
The aim of this study was to determine the antibacterial activity and potential use as seed disinfectant of Satureja hortensis L. essential oil, extracts (n-hexane, chloroform, acetone and methanol) and two pure compounds (carvacrol and thymol) against plant pathogenic bacteria. Antibacterial activity assays were carried out by disc diffusion metho...
Article
The essential oil isolated from the aerial parts of wild Turkish Satureja hortensis L. (summer savory) was analyzed by GC-MS, and carvacrol (54.7 %), y-terpinene (20.9 %), p-cymene (12.3 %), a-terpinene (2.0 %), and thymol (2.0 %) were found to be major components of the oil. Post-harvest decay of fruits caused by some fungi is still a most importa...
Article
Cadmium is a toxic metal widely used in industry and it enters the environment from electroplating, smelting, alloy manufacturing, pigments, plastics, cadmium-nickel batteries, fertilizers, pesticides, mining, pigments and dyes, textile operations, and refining industries. Considering the harmful effects of cadmium, it is necessary to remove cadmiu...
Article
In this study; a total of 77 Bacillus cereus strains, isolated from 4 different food samples (raw milk, chicken, cereal and meat), were tested for their susceptibility against S. hortensis essential oil. In addition, it was investigated whether there is any relationship between fatty acid methyl esters of B. cereus strains and their susceptibility...
Article
Full-text available
There are many studies focused on antibacterial and antifungal activity of the essential oil or extracts of Satureja hortensis. But their modes of action against microorganisms are poorly understood. The first objective of this study was to determine the antibacterial activity of Satureja hortensis essential oil (500 μl/ml) against Salmonella enter...
Article
The chemical compositions of hydrodistilled essential oils and n-hexane extracts from Origanum acutidens, O. rotundifolium and O. vulgare were analyzed by GC and GC-MS. Essential oils and hexane, chloroform, acetone and methanol extracts were tested against 25 plant pathogenic bacterial strains. Essential oils showed a wide spectrum of antibacteria...
Article
In this study, a total of 77 Bacillus cereus isolates were obtained from four different food samples (58 raw milks, 8 chickens, 7 cereals and 4 meats) consumed in Turkey by using Chromogenic Bacillus Cereus Agar (Oxoid, CM1036). They were tested for susceptibilities to a total of 10 different antibiotics (penicillin, oxacillin, sulphamethoxazole, r...
Article
It is known that many plant essential oils have been shown to possess antimicrobial activity. The aim of this study was to investigate the comparative antibacterial activities of the essential oils from seven different plant species collected from Turkey and two pure compounds against S. enteritidis at 25° and 35°C to determine the major compounds...
Article
Full-text available
The aims of this study were to examine the chemical composition of the essential oils and hexane extracts of the aerial parts of Satureja spicigera (C. Koch) Boiss., Thymus fallax Fisch. & CA Mey, Achillea biebersteinii Afan, and Achillea millefolium L. by GC and GC-MS, and to test antibacterial efficacy of essential oils and n-hexane, chloroform,...
Article
The objective of this work is to determine the microbiological and chemical characteristics of Çeçil cheese. A total of 15 samples of Çeçil cheese were collected from individual households in Erzurum, Turkey. In the samples, average of total aerobic mesophilic bacteria, yeast and moulds, lactic acid bacteria (LAB) and Staphylococcus aures were dete...
Article
Antagonistic activity of 24 selected bacterial strains detected by previous microbiological studies to Aspergillus flavus was tested in vitro and in vivo conditions. Within 24 strains, only ten strains showed remarkable inhibition zone (6-34 mm) against the pathogen in assays carried out in Petri plates. Both cell suspension and culture filtrates o...
Article
Full-text available
Five different plant essential oils (Satureja hortensis, Thymus sipyleus ssp. rosulans, Thymus haussknechtii, Origanum rotundifolium (cultured form) and Origanum acutidens (wild and cultured form)) and their two major constituents carvacarol and thymol were evaluated for antibacterial activity against food-borne Gram negative (Escherichia coli, Pse...
Article
The essential oil and methanol extract of Satureja hortensis were tested for antifungal activity against Aspergillus flavus in vitro on Petri plates and liquid culture, and under storage conditions. The oil showed strong antifungal activity based on the inhibition zone and minimal inhibitory concentration values against the pathogen on Petri plates...

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