Nenad L. Ignjatovic

Serbian Academy of Sciences and Arts · Institute of Technical Sciences

Low targeting efficiency and fast metabolism of antineoplastic drugs are hindrances to effective chemotherapies and there is an ongoing search for better drugs, but also better carriers. Steroid derivatives, 3β-hydroxy-16-hydroxymino-androst-5-en-17-one...

Taking advantage of the flexibility of the apatite structure, nano- and micro-particles of hydroxyapatite (HAp) were doped with different combinations of rare earth ions (RE3+ = Gd, Eu, Yb, Tm) to achieve a synergy among their magnetic and optical properties and to enable their application in preventive medicine, particularly diagnostics based on m...

Purpose Biofilm-free implant surface is ultimate prerequisite for successful soft and bone tissue integration. Objective of the study was to estimate the effects of argon plasma healing abutment pre-treatment (PT) on peri-implant soft-tissue phenotype (PiSP), inflammation, plaque accumulation and the microbiome (PiM) between non-treated (NPT) and t...

Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegrati...

Mammal bone tissue is mainly composed of an inorganic component consisting of calcium phosphate (CaP) ceramics, predominantly calcium hydroxyapatite (HAp), in addition to the organic component. Properties of HAp enable the modification of its structure, surface, design of particle size at the micro and the macro levels, hybridization with polymers,...

Sn-Pd electrocatalysts with a constant atomic ratio of 60 at.% Sn‒40 at.% Pd suitable for potential application in direct ethanol fuel cells were synthesized using a novel two-step electrodeposition method. First, Sn was electrodeposited in various forms of dendrites, from spear-like and needle-like to individual fern-like dendrites to a network of...

Hydroxyapatite (HAp) is the major component of all boney tissues in mammals. Because of this omnipresence in the living world, HAp possesses an exceptional biocompatibility. The downside of this omnipresence, however, comes in the form of its mild to moderate biological activities. One means of augmenting these activities involves the doping of HAp...

Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nan...

Dense calcium phosphate-based ceramics were fabricated to be used as targets for pulsed laser deposition (PLD). Nanostructured cobalt-substituted hydroxyapatite (Co:HAP) was used as a starting powder. To vary phase composition and microstructure of targets, two sintering approaches were applied, conventional (CS) and two-step sintering (TSS). The o...

Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a...

Cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line is elucidated. ACP/TiO2 and ACP/TiO2/ChOL are deposited onto Ti by novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no signifi...

Over the past decades, development of engineering sciences has incredibly contributed to advancements in medicine by production of numerous devices for diagnostics and treatment. In the middle of the twentieth century, a new scientific field, biomedical engineering (BE), was established, which has developed into an extremely complex scientific disc...

MRS Serbia 1 History 2 The idea of founding the Society 2 Birth and the first days of YU-MRS (MRS-Serbia) 3 YUCOMAT on the list of world-renowned conferences 5 Perspectives and future activities 8 Committee members of YUCOMAT conferences (1995 - 2020) 9 Statistics 13 List of plenary speakers 15 MRS-Serbia Awards Award for a Lasting and Outstand...

Functional nanomaterials have held a steady position at the frontier of materials science and engineering in the 21st century. "Molecular Designing of Nanoparticles with Controlled Morphological and Physicochemical Characteristics and Functional Materials Based on Them” was the title of the research project funded by the Ministry of Education, Scie...

Tetrahydropyrimidines are a class of azaheterocycles, also called Biginelli hybrids (obtained from the Biginelli reaction), that have attracted an enormous interest in the medicinal chemistry community in recent years, due to a broad biological activity, such as anticancer, antiviral, anti-inflammatory, antidiabetic, antituberculosis activities, et...

In this paper novel in situ one-step simultaneous anaphoretic deposition process of amorphous calcium phosphate (ACP) and titanium oxide (TiOx) with and without chitosan oligosaccharide lactate (ChOL) on titanium substrate was performed. The coatings were investigated by SEM, XRD and FTIR techniques, whereas roughness and adhesion were measured. It...

The optimization of the anodization process of Ti substrate for in situ synthesis of hydroxyapatite/titanium oxide composite coatings on titanium substrate was accomplished. The anodization was performed under 30, 60 and 90 V cell voltage, and the morphology of treated surface, as well as linear and surface roughness, were analysed by field emissio...

Production of copper powders by the potentiostatic electrolysis under different hydrogen evolution conditions was investigated. Copper powders were characterized by the scanning electron microscope (SEM), X-ray diffraction (XRD), particle size distribution (PSD), and by the determination of the specific surface area (SSA) of the formed powders. Dep...

Reconstruction of bone defects with the use of biomaterials based on hydroxyapatite (HAp) has been a popular approach in medicine and dentistry. Most often the process of new bone formation is analyzed with the focus only on the region of the reconstructed defect. The effects of the therapy on distant organs have been rarely reported in the literat...

In-situ synthesis of HAp/TiO2 coating on titanium was performed via anaphoretic deposition of HAp and simultaneous anodization of Ti to produce highly adherent and strengthened composite coating. The prepared coatings were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and electron dispersi...

Three different forms of Cu powder particles obtained by either galvanostatic electrolysis or a non-electrolytic method were analyzed by a scanning electron microscope (SEM), X-ray diffraction (XRD) and particle size distribution (PSD). Electrolytic procedures were performed under different hydrogen evolution conditions, leading to the formation of...

Transformations between amorphous and crystalline apatite mechanistically govern some of the most essential processes in bone metabolism, including biomineralization and bone remodeling. Fundamental understanding of this phase transition can help us gain control over the formation and dissolution of boney tissues in vivo and utilize that knowledge...

Introduction: Composite biomaterials based on nano hydroxyapatite (HAp) are the subject of numerous studies in reconstructive medicine. Multifunctional and nanoparticulate systems based on HAp and biodegradable polymers are successfully designed as systems for controlled and systemic drug delivery suitable for use in reconstructive medicine [1, 2]....

An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to tar...

Nanotechnology is an enabling technology with great potential for applications in stem cell research and regenerative medicine. Fluorescent nanodiamond (FND), an inherently biocompatible and nontoxic nanoparticle, is well suited for such applications. We had developed a prospective isolation method using CD157, CD45, and CD54 to obtain lung stem ce...

A review of recent advances in instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue is presented in this paper. In recent years, biomaterials attracted great attention primarily because of the wide range of biomedical applications. This paper focuses on the practical aspects of inst...

The interdisciplinary research team implemented the program titled “Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them” (MODENAFUNA), between 2011 and 2016, gaining new knowledge significant to the further improvement of nanomaterials and nanotechnologies. It...

Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to e...

Thanks to its special caracteristics, such as relativly easy chemical modification of structure and surface, calciumphosphates (CaP) especially hydroxyapatite (HAp) is found widely used in various fields of biomedicine. Beside of the ease chemical modification, which includes metal doping, enhancement of properties of the HAp can be accomplished by...

Changes in calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA) nanoparticles and ions content after incubation in Dulbecco's Modified Eagle Medium (DMEM) at 37ºC were examined in this study. Fourier transform infrared (FTIR) spectroscopy provided molecular structure information related to CP/PLGA nanoparticles and the semi-quantitative analy...

Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hy-droxyapatite (HA) nano-and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthe...

Implantation of a biomaterial is one of the important trends in solving the problem of bone tissue loss. Calcium hydroxiapatite (HAp), as the most representative bone component is a serious candidate for such implantations. The synthetic polymer poly-L-lactide (PLLA) in HAp/PLLA is often used as a polymeric material, with a role in the substitution...

In the field of oral implantology the loss of bone tissue prevents adequate patient care, and calls for the use of synthetic biomaterials with properties that resemble natural bone. Special attention is paid to the risk of infection after the implantation of these materials. Studies have suggested that some nanocontructs containing metal ions have...

In the field of oral implantology the loss of bone tissue prevents adequate patient care, and calls for the use of synthetic biomaterials with properties that resemble natural bone. Special attention is paid to the risk of infection after the implantation of these materials. Studies have suggested that some nanocontructs containing metal ions have...

Hydroxyapatite (HAp) is an extensively studied material with known biocompatible and osteoconductive properties in bone tissue reconstruction. The improvement of the osteogenetic potential of HAp has been tested through modification of its structure, by replacing Ca2+ ions with Co2+ ions. In our study, we comparatively analyze the osteogenetic pote...

Hydroxyapatite (HAp) is an extensively studied material with known biocompatible and osteoconductive properties in bone tissue reconstruction. The improvement of the osteogenetic potential of HAp has been tested through modification of its structure, by replacing Ca2+ ions with Co2+ ions. In our study, we comparatively analyze the osteogenetic pote...

Background/Aim. Different bone substitutes have been used for filling and reparation of intrabony defects. The aim of this study was to compare nanocrystalline material, biphasic calcium phosphate poly-DL-lactide-co-glycolide (BCP/PLGA) with deproteinised bovine bone (DPBB) and p-tricalcium phosphate (beta-TCP) in the treatment of periodontal intra...

Objective The aim of this study was to evaluate the clinical outcome of a nano crystalline material - biphasic calcium phosphate poly-DL-lactide-co-glycolide (BCP/PLGA) and to compare it with deproteinized bovine bone (DPBB) and β-tricalcium phosphate (β-TCP) in the treatment of human periodontal intra-bony defects. Design The study included 24 p...

Fullerenols are polyhydroxylated, water soluble derivatives of fullerene C60, with potential application in medicine as diagnostic agents, antioxidants or nano drug carriers. This paper describes synthesis and physical characterization of a new nanocomposite hydroxyapatite/fullerenol. Surface of the nanocomposite hydroxyapatite/fullerenol is inhomo...

Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomoleculs such are proteins, adsorbs these moleculs, and leads to formation of nanopartical-biomoelcular comlex known as...

Inverse gas chromatography at infinite dilution was applied to determine the thermodynamic interactions of poly(L-lactide) (PLLA) and the composite of biphasic calcium phosphate and PLLA (BCP/PLLA). The specific retention volumes, of 11 organic compounds of different chemical nature and polarity (non-polar, donor or acceptor) were determined in the...

Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morpho...

Bone tissue reconstruction and reparation is big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Bone marrow is a complex tissue composed of hematopoietic and stromal mesenchyme stem cell (BMSCs) with a differentiation potential to adipogenic, fibroblastic, reticular, osteogenic a...

The aim of our study was to evaluate the effect of designing ketoprofen-loaded nanosized spheres and combining them with solid silicon microneedles for enhanced and sustained percutaneous drug delivery. Ketoprofen-loaded nanoparticles (KET-NP) were designed by modified solvent displacement method, using poly (D, L-lactic acid) (PDLLA). All prepared...

The lack of bone in the jaw tegmenta inflicts major problem and leads to an inability to adequately treat stomatoprosthetic patients. If the bone tissue damage is minor, the balanced activities of osteoblasts and bone osteoclasts can repair it independently. If the damage is bigger it is necessary to support the biological potential to repair the b...

The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood cl...

Cholecalciferol, vitamin D3, plays an important role in bone metabolism by regulating extracellular levels of calcium. Presented here is a study on the effects of the local delivery of cholecalciferol (D3) using nanoparticulate carriers composed of hydroxyapatite (HAp) and poly(d,l-lactide-co-glycolide) (PLGA). Multifunctional nanoparticulate HAp-b...

Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the...

Young Researchers' meetings are held annually late in December since 2002 and they are organized by the Materials Research Society of Serbia. Originally conceived as seminars, since 2007 these meetings were transformed into conferences. The previous ten meetings featured presentations based on the research of various young scientists from Serbia, B...

Huge range of tested biomaterials in recent decades has emerged as an ideal scaffold for cell growth, but few have demonstrated clinical efficacy. Among them, synthetic hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is the most promising because of its biocompatibility, bioactivity, and osteoconductivity. Biocompatibility represents the primary concern for...

We have examined the effect of extracts of the calcium phosphate/poly(DLlactidecoglycolide) (CP/PLGA) and cobaltexchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 370C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assa...

Bone loss during the systemic osteoporosis has an important role in dentistry and medicine. The aim of the study was the application of bioactive microand nanocomposite materials, alone, and in combination with autologous plasma in osteoporotic jaw bones of rats with artificially induced osteoporosis. The effect of these composites was measured by...

In recent years, calcium hydroxyapatite (HAp) and its substituents are increasingly used in dentistry and medicine. The influence of nanoparticles Ca / Co-HAp in strengthening weak osteoporotic bone jaw tegmenta was tested in an experimental model. The study was conducted on Wistar soy rats, aged 6-8 weeks. The biomaterial was implanted in the oste...

Unlabelled: Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent in...

The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the i...

MTT test is a widely used method for examination of material biocompatibility in vitro. Nanomaterials, because of their specific structure, usually require a special approach in this kind of examination. The aim of this study was to examine the effect of calcium phosphate/poly-(DL-lactide-co-glycolide) (N-CP/DLPLG) nanoparticles suspension on viabi...

The surface properties of PLGA/HAp core-shell nanoparticles loaded with clindamycin obtained by an ultrasonic processing method and their changes under the simulated physiological conditions during the degradation process (when the morphology is changed starting from the nanospheres, over micrometer-sized plate-like films to a porous network) were...