Nemesio Villa-Ruano

Meritorious Autonomous University of Puebla | BUAP · Centro Universitario de Vinculación y Transferencia de Tecnología

El cultivo de la fresa (Fragaria × ananassa) es de gran importancia debido a su calidad nutrimental, aportes a la salud, impacto económico y en la generación de nuevas estrategias para el mejoramiento de sus rendimientos y características morfo-fisiológicas. Esta revisión bibliográfica tiene el objetivo de recopilar y mostrar el estado del arte act...

Introducción: El sistema inmunitario responde contra microorganismos patógenos, entre bacterias, virus, protozoos, parásitos y compuestos derivados de estos. Sin embargo, se debe silenciar esta respuesta a microorganismos comensales que colonizan y/o compuestos inocuos derivados de alimentos; como es el caso de los edulcorantes no nutritivos, tanto...

Solenopsis geminata is an aggressive pest of manzano pepper (Capsicum pubescens) crops. Herein, we report on the volatilomics profiling of manzano pepper plants obtained during S. geminata infestation by SPME‐GC/MS. As a result, sixty‐eight volatile organic compounds were identified from ants, non‐infested plants, and infested plants, including ter...

The chemical variation of the annual essential oils from Pimenta dioca (PdEOs) grown in the Northern Highlands of Puebla, Mexico, and their biological activities were determined. Eugenol (>50%) and beta-myrcene (>16%) were the main volatiles contained in the annual PdEOs (2022-2024) extracted from fruit and leaves. PdEOs showed inhibitory propertie...

A therapeutic strategy to treat Type 2 diabetes mellitus (T2D) is focused on controlling postprandial glucose levels by inhibiting α-amylase and α-glucosidase. These enzymes are essential to convert assimilable carbohydrates into glucose. Nutraceuticals contained in plant foods exert inhibitory properties on these enzymes, which are comparable to s...

The Capsicum genus includes several cultivated species that release complex blends of volatile organic compounds (VOCs) associated with their unique aroma. These VOCs are essential info-chemicals in ecological interactions. In this review, we describe how the volatilomic profiling naturally varies based on specific plant organs and genotypes as wel...

The flowers of Yucca aloifolia (“flor de izote”) are considered a millenary food in the Northeastern Highlands of Puebla, Mexico. The present investigation reports on the chemical and biological activities of the hydroalcoholic extract (YAHF) obtained from this edible source. HPLC-MS profiling revealed twenty bioactive phenolic compounds with chlor...

Los quelites de México y otros alimentos nativos de origen vegetal representan una fuente de moléculas bioactivas cuyo consumo genera beneficios para la salud de la población. En esta revisión de carácter divulgativa se describen a los principales compuestos con actividad nutracéutica encontrados en plantas comestibles consumidas dentro del territo...

The 1H NMR metabolomics profiling of six edible mushrooms consumed in the northeastern highlands of Puebla, Mexico is presented. These fungi were morpho- and molecularly identified as Infundibulicybe squamulosa, Amanita jacksonii, Lepista nuda, Russula delica, Russula brevipes, and Lactarius indigo. The chemical profiling confirmed the presence of...

Leafcutter ants (Atta spp.) are one of the mos t economically harmful pests in agriculture, considered dominant in the Neotropics and South America. Mature colonies of A. mexicana have a great economic impact on Mexico's agriculture. Microbial agents in the form of biopesticides are an effective component of integrated pest management (IPM) strateg...

During the spring of 2020, fruit rot was observed on strawberry cv. Camino Real in two 1,000 m 2 greenhouses belonging to the Santa Cruz Analco community, Puebla, Mexico.

Herein, we present an integrative investigation on the nutritional and nutraceutical potential of Lactarius indigo, Clitocybe nuda, Clitocybe subclavipes, Russula delica, Russula brevipes, Clitocybe squamulosa, and Amanita jacksonii, which are edible mushrooms consumed in the Northeastern Highlands of Puebla, Mexico. The content of protein oscillat...

Herein we report on the generation of hairy root lines of P. scaberrima able to produce hernandulcin (HE), a non-caloric sweetener with nutraceutical properties. From ten different lines analyzed, three synthesized up to 100 mg·L-1 HE under the batch culture conditions standardized in this investigation. Adding elicitors (salicylic acid, chitin, Gl...

Herein we describe the in situ inhibitory activity of three hybrid films (FL1, FL2, and FL3) against two wild strains of Colletotrichum gloeosporioides and Penicillium digitatum as causal agents of rot in Persian limes. The films FL2 and FL3 contained 1.0 and 1.3 % weight/volume Litsea glaucescens essential oil (LgEO) and significantly (p < 0.05) d...

Repurposing of approved drugs allows strong savings in time and investment. Rimantadine is an FDA-approved drug for prevention and treatment of influenza A infection. Patent US2021330605 describes the use of rimantadine, an adamantane derivative, for the treatment of melanoma, breast cancer and head and neck squamous cell carcinoma. Rimantadine inh...

We present the inhibitory properties of the Solanum nigrescens anthocyanin fraction (SNAF) and its major constituents on alpha-glucosidase (AG), pancreatic lipase (PL), HMG-CoA reductase, and ornithine decarboxylase (ODC). The effect of SNAF was simultaneously evaluated in ICR male mice exposed to triglyceride charge test (TCT). HPLC-MS profiling r...

Repurposing of approved drugs in a new strategy to combat cancer that leads to savings in time and investment. Atovaquone is a US FDA approved drug for treatment of Pneumocystis carinii pneumonia and malaria. Patent US2023017373 describe the use of mito-atovaquone for the treatment of several types of cancer. Mito-Atovaquone demonstrated antiprolif...

The native ant Solenopsis geminata (Fabricius, 1804) is a continuous pest of manzano pepper plants (Capsicum pubescens Ruiz and Pav.) cultivated in the northern highlands of Puebla, Mexico. This is the first report of S. geminata substantially affecting planations of manzano pepper in disturbed mesophyll rainforest of Mexico. This study is the firs...

We present the inhibitory properties of the R. pompana anthocyanin fraction (RPAF) and its major constituents on alpha-glucosidase (AG), pancreatic lipase (PL), HMG-CoA reductase, and ornithine decarboxylase (ODC). The effect of RPAF was also evaluated in ICR male mice subjected to oral glucose tolerance test (OGTT) and hypercaloric/atherogenic die...

Little is known on the use of biodegradable films for the control of diseases in crops of agricultural importance. Consequently, the objective of this work was to determine the efficacy of chitosan hybrid films impregnated with thyme essential oil on Hass avocado fruits previously infected with Fusarium solani isolated from the northeastern highlan...

There are several by-products obtained in agricultural systems that can contribute to small-scale production systems within backyards, in this sense, the production of oyster mushrooms (Pleurotus ostreatus) has been integrated into the rural family economy in Mexico. After harvesting the fruiting bodies, the remainder is known as sold-out substrate...

Resveratrol (RV), carnosic acid (CA) and hernandulcin (HE; a non-caloric sweetener) are envisioned as promising nutraceuticals to design new functional foods for improving lipid and carbohydrate metabolism. This study aimed to investigate the in vitro inhibitory effect of these molecules on specific enzyme targets and their capacity to improve dist...

Hairy root cultures of Centella asiatica–based coupled with exogenous application of methyl-jasmonate and coronatine are suitable bioproduction platforms to overproduce this plant-occurring high-valuable metabolite. These two molecules orchestrate cellular responses in metabolism that have not been yet deeply explored in hairy roots of this plant....

For more information please visit: https://www.mdpi.com/journal/metabolites/special_issues/JZR9M4U27V

Purslane (Portulaca oleracea L.) has a high content of nutrients and medicinal effects that depend on the genotype, harvesting time, and production system. The objective of the present research work was to elucidate the NMR-based metabolomics profiling of three native purslane cultivars from Mexico (Xochimilco, Mixquic, and Cuautla) grown under hyd...

Lepidopterism caused by caterpillar contact is considered a public health problem around the world. The local and systemic responses of this pathology include short-and long-term inflam-matory events. Although the proteolytic activity of the venoms from caterpillars is strongly associated with an inflammatory response in humans and murine models, f...

Lepechinia meyenii is a medicinal plant specialized in the biosynthesis of different types of antioxidants including the diterpenes carnosic (CA) acid and carnosol (CS). Herein we present the results of plant tissue culture approaches performed in this medicinal plant with particular emphasis on the generation and evaluation of a cell suspension sy...

The northern highlands of Puebla harbor several plant foods consumed since pre-Columbian times. Most of the native vegetables from this geographical region are still uncharacterized at the nutraceutical level. This investigation was focused on the elucidation of chemical and biochemical properties of the edible organs from Rhamnus pompana, Solanum...

The leaves of Piper auritum Kunth (“Hoja Santa”) have been consumed for centuries by native people of central and southern Mexico as a fresh vegetable or condiment. Herein we present the result of the 1H NMR metabolomics profiling of three accessions of P. auritum harvested in three different provinces of Mexico (Puebla, Tlaxcala, and Oaxaca). The...

Celery is a vegetable widely consumed as a condiment to prepare diverse dishes around the world. Nevertheless, this plant is susceptible to the attack of several phytopathogens including those of the Fusarium genus which is translated into devastating losses for the production chain. Herein we report on the metabolic changes produced during the cel...

The manzano pepper (Capsicum pubescens) is an exportation product that generates substantial earnings for local producers in Mexico. Herein we report on the most relevant metabolic changes that occur during the postharvest rot of manzano peppers caused by Fusarium temperatum. Simultaneously, we describe the effect of the Zanthoxylum limoncello leaf...

The aim of the present study was to synthesize isoflavone-enaminones 3a-c and 7-alkoxy-isoflavones 4a-c, evaluate their inhibition of α-glucosidase, and analyze the bioisosteric effect of the presence versus absence of aromatic moieties in these benzopyran derivatives. All the test compounds exhibited greater inhibition of α-glucosidase than the po...

Avocadoes are a rich source of nutrients and nutraceuticals that preserve human health. Nevertheless, this fruit is susceptible to phytopathogen infection during the postharvest period causing severe economic losses. Herein, we report on the in situ antifungal assessment of biodegradable films impregnated with the essential oil of Cinnamomum verum...

Fundamentos: El estado nutricional de la población se ve influenciado por diversos factores económicos, sociales y culturales, especialmente en zonas indígenas. La creación de programas como “La Casa Para Niño Indígena” (CNI) proporciona alimentación a estas poblaciones vulnerables. El presente trabajo determinó el perfil nutricional de la dieta pr...

Hass avocadoes are one of the most popular fruits consumed worldwide because of their nutritional and nutraceutical content. Nevertheless, these fruits are susceptible to phytopathogen attacks that decrease fruit quality during the postharvest period. Herein we present the results of the in situ fungistatic activity of four hybrid films (FT1–FT4) m...

p>The northern highlands of Puebla are considered a new potential area for the cultivation of Hass avocado. Then, the emergence of new phytopathogens related to this geographical area is latent. The aim of this investigation was to isolate the microorganisms linked to the avocado fruit rot recently recorded by the producers of this locality in 2021...

p> Background : Species of the Fusarium genus are considered as devastating phytopathogens of onion crops around the world. Objective : This work aimed to know the causal agent of basal rot in onion crops from Puebla-México recorded in 2019. Methodology : The causal agent was isolated from diseased samples by tissue incubation in Potato Dextrose Ag...

Rhizopus rot is a common postharvest disease that causes devastating economic losses for peach producers around the world. Thus, new alternatives for the control of this disease are required. Herein we report on the obtainment of a new hydrotalcite/shilovite (HT/Sh) composite with in situ fungistatic properties on Rhizopus stolonifer. The protectiv...

The control of postharvest diseases in tomatoes using hybrid films represents an efficient and inexpensive alternative to avoid economic losses. This work reports on the design, characterization, and in situ application of hybrid films containing chitosan combined with the leaf essential oil of Schinus molle (SmEO) as antifungal agents. These mater...

Cera pepper ( Capsicum pubescens ) is an exotic fruit considered as a rich source of nutraceuticals with known benefits for human health and also an economic resource for local producers in Mexico. The present investigation reports on the in vitro and in situ antifungal activity of the essential oil from Mentha piperita and its two major volatiles...

Puebla is one of the provinces in Mexico that produces manzano pepper (Capsicum pubescens). Recently, the producers of manzano pepper from the northern highlands of Puebla reported an aggressive fruit blotch that caused severe damage and substantial economic losses. Symptoms were rot spots with mycelial growth at the center of the spot, necrosis in...

The seeds of Phaseolus vulgaris are a rich source of protein consumed around the world and are considered as the most important source of proteins and antioxidants in the Mexican diet. This work reports on the 1H NMR metabolomics profiling of the cultivars Peruano (FPe), Pinto (FPi), Flor de mayo (FM), Negro (FN) and Flor de junio (FJ). Total pheno...

ent-Kaurenoic acid (KA) is a tetracyclic diterpene with known hypoglycemic properties and is a key intermediary in the biosynthesis of giberellins. This work presents the results of enzymatic assays using endosperm extracts from Cucurbita pepo and Phaseolus vulgaris in combination with commercial mevalonolactone for the obtainment of ent-kaurene, i...

Hernandulcin (HE) is a non-caloric sweetener biosynthezised by the Mexican medicinal plant Phyla scaberrima . Herein we present the results of HE production through cell suspensions of P. scaberrima as well as the influence of pH, temperature, biosynthetic precursors and potential elicitors to enhance HE accumulation. The incorporation of mevalonol...

Melanin is a bio-pigment present in microorganisms, plants, and animals. In humans, melanin has several functions, one of the most important being protection against pathogen microorganisms. With this in mind and considering that clays also have the ability to act against microorganisms, the major aim of this study was to associate natural melanin...

Beans are the most cultivated legume in the world. In Mexico, it is the second most important crop after corn (FAO 2020; SIAP 2020). Bean plants “Flor de Mayo M38” variety were affected by a foliar disease during the agricultural cycle 2019 in Puebla-Mexico (19°02'46.6" LN and 98°05'15.6" LO). Necrotic V- shaped lesions were observed on the margins...

Citation: Romero-Arenas, O.; Jara y Rivera, A.P.; Valencia de Ita, M..A.; Lezama, C.P.; Villa-Ruano, N.; Rivera, A. In Vitro Antimicrobial Activity of Cinnabarin on Xanthomonas campestris Abstract: Phaseolus vulgaris is the most legume cultivated in the world; in Mexico, it is considered the second most important crop after corn. The aim of this re...

The chemical factors that regulate the synthesis of resveratrol (RV) in filamentous fungi are still unknown. This work reports on the RV production by Arcopilus aureus MaC7A under controlled conditions and the effect of amino acid precursors (PHE and TYR), monoterpenes (limonone, camphor, citral, thymol, menthol), and mixtures of hydrolytic enzymes...

p>La cebolla es la segunda hortaliza de mayor producción en el mundo. En México constituye un cultivo de gran importancia económica en diversos estados. El objetivo de la presente investigación fue identificar y caracterizar morfológicamente la cepa CFbC asociada a la podredumbre basal del cultivo de cebolla, así como determinar la capacidad antagó...

The roots of Zaluzania montagnifolia are traditionally boiled to obtain an aqueous infusion to treat type 2 diabetes mellitus in Oaxaca, México. The objective of this work is to estimate the hypoglycemic effect of the root infusion from Zaluzania montagnifolia in healthy volunteers. One hundred and fifty eight healthy patients (70-100 mg dL-1 gluco...

Preventive measures based in the control of insect vectors are considered as the best choice to decrease the incidence of insect-borne diseases. Herein we report on the volatile content of the leaf essential oils from Marina neglecta, a medicinal plant distributed in the tropical regions of southern Mexico. In order to investigate the chemical vari...

Resveratrol, carnosic acid and hernandulcin are considered as nutraceuticals with potential application against pandemic health problems such as obesity, type 2 diabetes mellitus and diverse types of cancer. Basic and applied studies centered on metabolic engineering have emerged to scale the production of these molecules. The natural production of...

Brickellia veronicifolia is a native Asteraceae from Mexico that persists in fragmented habitats. This investigation reports the genetic and chemical diversity of B. veronicifolia. The diversity analysis based on iPBS markers showed an averaged Shannon index (S) of 0.3493, a Nei genetic diversity (h) of 0.2256, and a percentage of polymorphic loci...

Objective To estimate the correlation between indices of diet quality (DQIs), insulin sensitivity (QUICKI) and resistance (HOMA-IR), waist circumference (WHR) and body mass (BMI) and the alleles and genotypes of the TJP1 SNP rs2291166 and the VNTR of ATXN2 in adolescent patients. Subjects and method The study enrolled 85 subjects aged 10–20 years,...

The essential oil from the annual plant Lepidium virginicum L. was chemically characterized in three consecutive years (2018‐2020). The essential oils were evaluated in vitro and in situ on the causal agent of anthracnose in tamarillo fruits ( Solanum betaceum ). The main volatile constituents were phenylacetonitrile (>60%), linalool (>10%), limone...

This work reports on the metabolic fingerprinting of ten new races of Capsicum annuum cv. jalapeño using 1H NMR based metabolomics coupled to machine learning projections. Ten races were classified and evaluated according to their differential metabolites, variables of commercial interest and by multivariate data analysis/machine learning algorithm...

Introducción: Montanoa tomentosa es un recurso fitogenético con propiedades medicinales utilizada en México. A pesar de su relevancia para la botánica, la información específica sobre la distribución de sus taxa infraespecíficos y los factores ambientales que influyen en su desarrollo aún es limitada. Objetivo: Informar sobre la distribución geogr...

Cnidoscolus multilobus (Pax.) I.M. Johnst (Euphorbiaceae) is a stinging plant widely distributed in the south of Mexico. Currently, chemical and biological properties of the endogenous fluid from the prickles of C. multilobus are still unknown. Thus, the objective of this work was to identify the inflammatory-inducing toxins contained in the prickl...

Pomegranate is an edible fruit with a valuable content of bioactive molecules. This work describes the metabolomic profiling of pomegranate samples collected from conventional and organic cultures by using a combined 1H NMR-based and multivariate analysis protocol. The results of Principal Component Analysis (PCA) and Orthogonal Projections to Late...

Introduction: PD-L1 and PD-1 are two immune checkpoints and their presence in various types of tumours is related to a poor prognosis; this makes them highly relevant targets in the development of new therapies. Patent US2019010232 describes bispecific anti-PD-L1/PD-1 antibodies made with Azymetric technology. Areas covered: Three bispecific antibo...

Dyssodia decipiens is one of the seven recognized species within the Dyssodia genus which has economic and social relevance in Mexico. Herein we report on the volatile characterization and pharmacological activity of D. decipiens . The annual essential oils (2017‐2019) contained verbenone (30.8‐60.9%), limonene (5.6‐35.5%), β‐caryophyllene (7.8‐19....

Featured Application: This work was focused in the generation of six yeast lines able to produce resveratrol from p-coumaric acid. Industrial use of these strains may be contemplated. Abstract: Resveratrol is a nutraceutical with relevant benefits to human health. This investigation reports on the generation and evaluation of six recombinant yeast...

Purpose: To determine the nutraceutical effect of mixtures containing Lactobacillus nagelii/betanin on the carbohydrate and lipid metabolism of mice. Methods: Lactobacillus nagelii and betanin were isolated from the raw materials. These components were subsequently assessed for their anti-hyperglycemic and hypolipidemic activities, and administered...

Introduction: Due to the primary role of PD-1 and LAG-3 in regulating the immune response in tumors, there is a need to develop therapies focused on the inhibition of PD-1 and LAG-3 in order to improve the immune response in patients with cancer. The authors of US2018326054 patent propose a method to eradicate cancer by using bispecific anti-PD-1/L...

This investigation reports on the biological control of Sphenarium purpurascens by using different concentrations of conidia from a native strain of Beauveria bassiana (MABb1) isolated in Puebla, Mexico under laboratory and in situ conditions. Formulations containing this native strain combined with two mesoporous materials (diatomaceous earth “D”...

Introduction: KIR is an inhibitory receptor expressed by natural killer cells that suppresses the immune response against tumor cells. There is a great need to discover and develop new therapies focused on inhibiting the action of KIR and consequently improving the immune response in the various types of cancer. Authors of US9879082 and US201820865...

Introduction: Nicotiana tabacum is a plant model intensively used in the bio-engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non-targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available. Objective: To compare the proton nu...

Resumen Fundamentos: En la etapa lactante los patrones de consumo alimentario (PCA) adecuados pueden mantener el correcto estado nutricional. La presente investigación buscó asociar el PCA durante la alimentación complementaria con el estado nutricional en lactantes. Métodos: Se realizó un estudio transversal analítico (mayo-julio de 2017) con pobl...

The infection of Capsicum annuum cv. mirasol by Candidatus Phytoplasma trifolii (16SrVI) causes devastating crop losses in northern Mexico. This study addresses the metabolomics profiling of mirasol chili peppers (Capsicum annuum cv. mirasol) infected by Candidatus Phytoplasma trifolii. For this study, 25 diseased fruits and 25 healthy fruits were...

In Mexico the commercial substrates more utilized for the forest seedlings production are Peat Moss, agrolite and vermiculite, however, their costs are high which makes the utility margins decrease; therefore, alternative substrates capable of promoting good quality seedlings are sought. The residues from the production of Shiitake mushroom (Lentin...

Introduction: LAG-3 is checkpoint inhibitor in cancer that coordinates the down regulation of the proliferation of antigen-specific lymphocytes. There is a great need to discover and develop new therapies focused on inhibiting the action of LAG-3 and consequently improving the immune response in the various types of cancer. Areas covered: The paten...

Series of novel chromonyl enaminones 1a–e and 2a–e and 3-alkylated chromones 3a–e were synthesized and evaluated in vitro as α-glucosidase inhibitors as well as antioxidant and antifungal agents. Antifungal activity was tested on strains of Candida albicans. Compounds 2a and 2d–e showed good inhibition of the α-glucosidase enzyme (IC50 = 5.5, 0.9,...

Approximately 90% of the chili peppers consumed in the world are harvested in Mexico. The present article describes the untargeted 1H NMR-based metabolomic profiling of 11 cultivars of Capsicum annuum species which are routinely consumed worldwide. The metabolomic fingerprinting detected via 1H NMR contained 44 metabolites including sugars, amino a...

This study describes the 1H NMR‐based metabolomics profiling of juices from citrus fruits harvested in the state of Veracruz, México. The hydrophilic profile of commercial lemons (Agrio and Persian), tangerines (Fremont and Mónica), oranges (Valencia and Washington Navel), and grapefruits (Red Ruby and Rio Red) was determined. According to our resu...