Nemanja Kuzmanovic

Nemanja Kuzmanovic
Julius Kühn-Institut · Institute for Plant Protection in Horticulture and Forests (Braunschweig)

PhD

About

54
Publications
15,686
Reads
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441
Citations
Citations since 2016
39 Research Items
419 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Introduction
Nemanja Kuzmanovic currently works at the Institute for Epidemiology and Pathogen Diagnostics (Braunschweig), Julius Kühn-Institut. Nemanja does research in Plant Pathology, with emphasis on bacterial diseases of important agricultural crops. Their most recent publication is 'Bacterial Diseases.'
Education
September 2001 - November 2005
University of Belgrade
Field of study
  • Phytomedicine

Publications

Publications (54)
Article
Full-text available
Four plant tumorigenic strains 932, 1019, 1078T and 1081 isolated from cane gall tumors on thornless blackberry (Rubus sp.) were characterized. They shared low sequence identity with related Rhizobium spp. based on comparisons of 16S rRNA gene (≤98%) and housekeeping genes atpD, recA and rpoB (<90%). Phylogenetic analysis indicated that the strains...
Chapter
Agrobacterium vitis is the primary causal agent of grapevine crown gall worldwide. Symptoms of grapevine crown gall disease include tumor formation on the aerial plant parts, whereas both tumorigenic and nontumorigenic strains of A. vitis cause root necrosis. Genetic and genomic analyses indicated that A. vitis is distinguishable from the members o...
Article
The plant tumorigenic strain NCPPB 1650T isolated from Rosa x hybrida, and four nonpathogenic strains isolated from tumors on grapevine (strain 384), raspberry (strain 839) and blueberry (strains B20.3 and B25.3) were characterized by using polyphasic taxonomic methods. Based on 16S rRNA gene phylogeny, strains were clustered within the genus Agrob...
Article
Full-text available
Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7T (CFBP 7436T, LMG 26435T), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674),...
Article
The rapid spreading of the disease during last few years highlighted the need of a quick, sensitive and reliable method for Pseudomonas syringae pv. actinidiae (Psa) detection, to find possible inoculum sources and limit the pathogen spreading. A PCR method, using new primers designed on the gene encoding a putative outer membrane protein P1, was d...
Article
Full-text available
Serious outbreaks of walnut deep bark canker were observed on young walnut trees (Juglans regia L.) in the northern part of Serbia during 2020. From the symptomatic walnut tissues, two types of bacterial colonies were isolated, predominantly, light cream, circular and smooth colonies, as well as small, yellowish, mucoid and convex ones. PCR analysi...
Article
Full-text available
Root nodules of legume plants are primarily inhabited by rhizobial nitrogen-fixing bacteria. Here, we propose two new Rhizobiales species isolated from root nodules of common sainfoin (Onobrychis viciifolia), as shown by core-gene phylogeny, overall genome relatedness indices, and pan-genome ana lysis. Mesorhizobium onobrychidis sp. nov. actively i...
Article
During May 2016 severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. A total of 22 strains were isolated: 16 from symptomatic raspberry shoots, two from asymptomatic raspberry leaves, and four from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks...
Article
Full-text available
Background Allorhizobium vitis (formerly named Agrobacterium vitis or Agrobacterium biovar 3) is the primary causative agent of crown gall disease of grapevine worldwide. We obtained and analyzed whole-genome sequences of diverse All. vitis strains to get insights into their diversification and taxonomy. Results Pairwise genome comparisons and phy...
Article
Full-text available
The Xanthomonas genus contains a set of diverse bacterial strains most of which are known for their pathogenicity on annual crops and fruit trees causing economically important plant diseases. Recently, five Xanthomonas strains were isolated from Agrobacterium-induced crown gall tissues of amaranth (Amaranthus sp.) and weeping fig (Ficus benjamina)...
Preprint
Root nodules of legume plants are primarily inhabited by rhizobial nitrogen-fixing bacteria. Here we propose two new Rhizobiales species isolated from root nodules of common sainfoin ( Onobrychis viciifolia ), as shown by core-gene phylogeny, overall genome relatedness indices and pan-genome analysis. Mesorhizobium onobrychidis sp. nov., actively i...
Article
Full-text available
The alphaproteobacterial family Rhizobiaceae is highly diverse, with 168 species with validly published names classified into 17 genera with validly published names. Most named genera in this family are delineated based on genomic relatedness and phylogenetic relationships, but some historically named genera show inconsistent distribution and phylo...
Article
Four strains isolated from the galls on blueberry plants (Vaccinium corymbosum) were characterized by using polyphasic taxonomic methods. Based on 16S rRNA gene phylogeny, strains were clustered within the genus Agrobacterium. Furthermore, multilocus sequence analysis (MLSA) based on the partial sequences of atpD, recA and rpoB housekeeping genes a...
Article
Full-text available
Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli , is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management...
Article
Full-text available
In summer 2019, widespread occurrence of crown gall disease caused by Agrobacterium spp. was observed on commercially grown ornamental plants in Southern Iran. Beside agrobacteria, pale yellow-pigmented Gram-negative strains resembling the members of Xanthomonas were also associated with crown gall tissues on weeping fig (Ficus benjamina) and Amara...
Article
Full-text available
Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were ana...
Article
The efficacy of the bio-fungicide Amylo-X®, based on Bacillus amyloliquefaciens subsp. plantarum (Bap) strain D747, was evaluated in vivo against Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit bacterial canker disease. By in vivo tests, under controlled conditions, Amylo-X® reduced the disease severity caused by Psa in Act...
Article
Full-text available
Agrobacterium tumefaciens species complex contains a set of diverse bacterial strains, most of which are well known for their pathogenicity on agricultural plants causing crown gall diseases. Members of A. tumefaciens species complex are classified into several taxonomically distinct lineages called “genomospecies” (13 genomospecies until early 202...
Article
Full-text available
Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The c...
Article
Full-text available
Pseudomonas syringae pv. actinidiae is an important pathogen of kiwifruit (Actinidia deliciosa), and bacterial canker of this host is managed by monitoring and chemical control strategies. The efficacy of the bio-pesticides Amylo-X® (based on Bacillus amyloliquefaciens subsp. plantarum strain D747) and Serenade Max® (strain QST713 of B. subtilis) w...
Preprint
Full-text available
The alphaproteobacterial family Rhizobiaceae is highly diverse, with 168 species with validly published names classified into 17 genera with validly published names. Most named genera in this family are delineated based on genomic relatedness and phylogenetic relationships, but some historically named genera show inconsistent distribution and phylo...
Article
Full-text available
Crown gall disease caused by diverse Agrobacterium species is one of the main biotic constraints in the ornamental plants industry in Iran (Mafakheri et al. 2017). In August 2019, Japanese spindle (Euonymus japonicus var. Green Rocket) plants showing crown gall symptoms were observed in a commercial greenhouse in Tehran, Iran. Infected plants were...
Preprint
Full-text available
Background Allorhizobium vitis (formerly named Agrobacterium vitis or Agrobacterium biovar 3) is the primary causative agent of crown gall disease of grapevine worldwide. We obtained and analyzed whole-genome sequences of diverse All. vitis strains to get insights into their diversification and taxonomy. Results Pairwise genome comparisons and phy...
Article
Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains...
Article
Crown gall is an economically important and widespread plant disease caused by tumorigenic bacteria that are commonly affiliated within the genera Agrobacterium, Allorhizobium, and Rhizobium. Although crown gall disease was reported to occur on rhododendron, literature data regarding this disease are limited. In this study, an atypical group of tum...
Article
Full-text available
Herein the members of the Subcommittee on Taxonomy of Rhizobia and Agrobacteria of the International Committee on Systematics of Prokaryotes review recent developments in rhizobial and agrobacterial taxonomy and propose updated minimal standards for the description of new species (and genera) in these groups. The essential requirements (minimal sta...
Article
Full-text available
Plasmids play a crucial role in the ecology of agrobacteria. In this study, we sequenced tumour-inducing (Ti) and opine-catabolic (OC) plasmids in three Rhizobium rhizogenes (Agrobacterium biovar 2) strains isolated from the same crown gall tumour on ‘Colt’ cherry rootstock and conducted comparative genomic analyses. Tumourigenic strains C5.7 and C...
Article
Full-text available
Three PCR methods, referred in this study as „conventional“, „nested“ and „chromosomal“ PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from differen...
Article
Full-text available
Xanthomonas euvesicatoria phage KΦ1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of KΦ1 is do...
Article
Plants are colonized by diverse microorganisms, which may positively or negatively influence the plant fitness. The positive impact includes nutrient acquisition, enhancement of resistance to biotic and abiotic stresses, both important factors for plant growth and survival, while plant pathogenic bacteria can cause diseases. Plant pathogens are ada...
Article
Pseudomonas sp. RU47 (RU47) is a rhizosphere-competent strain showing plant growth-promoting and biocontrol activities. In this study, the genome sequence of strain RU47 was obtained and phylogenetic and comparative genome analyses were performed. Multilocus sequence analysis (MLSA) coupled with the calculation of average nucleotide identity (ANI)...
Article
Full-text available
The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in...
Chapter
This book contains 20 chapters and is divided into 4 parts focusing on genetic resources and improvement, ecophysiology and production, pests and diseases and their management and harvesting, processing and utilization of sweet and sour cherries.
Article
Five Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different stone fruit rootstocks in Poland: strains F5.1(T) and F5.3 from Prunus avium F12/1, strains CP3.5 and CP17.2.1 from Prunus avium and strain AL5.1.8 from Prunus cerasifera. On the basis of 16S rDNA phylogeny, the strains cluster together and belong to th...
Article
Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of t...
Article
During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease....
Article
Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacte...
Article
Aims:To analyze genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an eight-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. Methods and results: By using random amplified polymorphic DNA (RAPD) PCR, strains studied were d...
Article
Serious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homogeneous biochemical and physiological characterist...
Article
In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 × 106 to 2.1 × 101 cells ml-1. Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isop...
Article
In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains...
Article
Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associate...
Article
In November 2010, a serious outbreak of crown gall disease was observed on 3-year-old grapevine (Vitis vinifera L.) cv. Cabernet Sauvignon grafted onto Kober 5BB rootstock in two commercial vineyards located in the South Banat District in Serbia. Large, aerial tumors were visible above the grafting point on grapevine trunks, and in most cases, the...
Article
Full-text available
Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedure...
Article
Full-text available
Due to an overlapping host range, similar symptomatology and many common characteristics,Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified.In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae,morsprunorum and persicae, we studied the suitability and differentiating potential...
Article
Full-text available
In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tum...
Article
Full-text available
Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard...
Article
Full-text available
Automated method of fatty acid analysis was used to identify and study heterogeneity of 41 Erwinia amylovora strains, originating from 8 plant species grown in 13 locations in Serbia and one in Montenegro. All strains contained 14:0 3OH fatty acid,characteristic for the “amylovora” group. According to fatty acid composition 39 strains were identifi...

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Projects (4)
Project
Crown gall is an economically important and widespread plant disease caused by tumorigenic agrobacteria, affecting various agricultural crops, primarily fruit species, grapevine and some ornamentals. The disease is caused by different members of the family Rhizobiaceae, distributed within genera Agrobacterium, Allorhizobium and Rhizobium. Nevertheless, it has been shown that crown gall tumors are not only inhabited by agrobacteria. However, previous studies were mainly based on isolation-based methods, which are mainly limited by the fact that large proportion of microorganisms cannot grow on standard nutrient media. Novel tools based on next-generation sequencing make it possible to get an insight into the total bacterial community of desirable ecological niche at relatively low cost. Therefore, the objective of the project is to unravel the diversity of bacteria associated with crown gall tumors on various agricultural crops in Serbia. The understanding of the diversity and role of the crown gall associated microbiota will contribute in development of more efficient management of crown gall disease in the future.
Project
Crown gall is an economically important and widespread plant disease caused by tumorigenic bacteria that are commonly affiliated within the genera Agrobacterium, Allorhizobium and Rhizobium. Atypical Rhizobiaceae strains representing a novel species described as Rhizobium tumorigenes sp. nov., were isolated from aerial tumors on blackberry in Serbia. R. tumorigenes is a new plant tumorigenic bacterium and the second tumorigenic species within the genus Rhizobium. More recently, an atypical group of tumorigenic agrobacteria belonging to the genus Rhizobium was identified as a causative agent of crown gall on rhododendron and blueberry in Germany. Our study suggested that tumorigenic bacteria associated with rhododendron and blueberries are most closely related to R. tumorigenes, with which they form a homogenous clade within the genus Rhizobium, that we named “tumorigenes” clade. Additionally, it cannot be excluded that the novel pathogen is distributed more widely. The first objective of the project will be focused on isolation and determination of the properties of antimicrobial compounds produced by potential newly isolated PGP (plant-growth-promoting) and biocontrol strains. This characterization is important also from the regulatory point of view. Many strains from different species within genus Bacillus and Pseudomonas produce a broad spectrum of bioactive peptides and other substances with significant potential for biotechnological applications. The second objective will be the development of the best formulation related to plant treatment. Crown gall disease is a common in nurseries and represents a significant problem. Disease can decrease plant vigor and yield drastically. In agriculture, bacterial inoculants are often applied through planting material prior to sowing. Inoculated scions are more likely to establish large enough rhizobacterial populations within the rhizosphere to produce notable beneficial effects on the plant. The third objective, as fundamental part, will include complete analysis of phytobiome of the treated and non-treated crops from the second part of the project. Advances in microbiome-enabling technologies will include high-throughput sequencing and computational biology in order to assess the community composition, function, and activity of both cultivable and non-cultivable organisms in the phytobiome. These results should address the following question: How can a phytobiome be manipulated to increase agricultural production in an environmentally sustainable manner? The best strains would be chosen for complete genome sequencing in order to explore complete metabolic profile of the strains and to decipher the mechanisms involved in the large host range, as well. In general, the ultimate goal of the project will be to investigate alternative strategies for the control of crown/cane gall disease of different fruit and ornamental species, caused by recently discovered and characterized group of tumorigenic bacteria (“tumorigenes” clade). In addition to existing isolates with proven biocontrol role, one of the main objectives will be isolation and characterization of novel endophytic isolates from the infected tumorigenic plants and investigation of their interaction with a novel plant pathogen. The purpose of the proposed research is to contribute to the development of sustainable agriculture and environmental protection. Among the important measures that contribute to the above aim is certainly one of well-designed, targeted use of plant protection products, which however, requires precise knowledge on diseases progress.