Nelly Besson

Terumo Blood and Cell Technologies · Therapeutic Systems

Background and Objectives The number of CD34+ cells collected in apheresis procedures depends mainly on the collection efficiency of the device and the blood volume processed. Large volume leukapheresis (LVL) can improve CD34+ cell yield and has previously been investigated using the COBE Spectra device (Terumo BCT, USA). Materials and Methods Thi...

Success of peripheral blood stem cell (PBSC) collections depends on patient biological parameters and stable apheresis device performance. We investigated product quality and factors influencing main apheresis procedure outcomes including CD34⁺ collection efficiency (CE), product volume or platelet CE. We also assessed different CD34⁺ cell yield pr...

Aim of the study Large volume leukapheresis (LVL) have shown to enhance CD34+cell yield collected. This study evaluated performance and safety of the Spectra Optia CMNC protocol (version 11) in adult and pediatric LVL. A prediction algorithm for CD34+cell yield was also tested. Study Design From March 2016 till September 2016,67 LVL in 46 adults an...

Introduction: Thrombocytapheresis is an alternative treatment beneficial in rare circumstances, when cytoreductive agents are contraindicated, drug therapy gave no response or the expected response would be too slow. Here we present a case of a pregnant woman who underwent 5 thrombocytaphereses using Spectra Optia device to reduce circulating plat...

A new continuous mononuclear cell collection protocol (CMNC) has been released on the Spectra Optia® platform (Terumo BCT, Belgium) for peripheral blood stem cell (PBSC) collections. Compared to MNC2, where MNC are collected by cycles, the CMNC offers a continuous collection & allows higher inlet-flow rates facilitating our “individualized high-flo...

Spectra Optia cell separator is a widely utilized apheresis system for collecting peripheral blood stem cells (PBSC). Because of the automation of the procedure, few input were required from operator and we found interesting to explore if some parameters could potentially affect the product collected and the performance of the device.

Bone marrow (BM) is one source of hematopoietic stem cells (HSC) transplantation. Before storing of both autologous and allogeneic bone marrow, it is strongly recommended to downsize bone marrow volume in order to reduce the cryogenic storage space but also to prevent DMSO toxicity during infusion. In addition, ABO-incompatible grafts may be RBC de...

King’s College Hospital collection facility has experience in using both the COBE Spectra® (Terumo BCT) and Spectra Optia® (Terumo BCT) cell separator platforms for Haematopoietic progenitor cells (HPC)-Apheresis (A) collection. Multiple factors influence the efficient collection of CD34+ HPC for patients undergoing mobilisation prior to Autologous...

Bis(monoacylglycero)phosphate (BMP), also called lysobisphosphatidic acid, is a phospholipid highly enriched in the internal membranes of multivesicular late endosomes, in which it forms specialized lipid domains. It has been suggested that BMP-rich membranes regulate cholesterol transport. Here, we examine the effects of an anti-BMP antibody on ch...

Lysobisphosphatidic acid (LBPA) is highly accumulated in specific domains of the late endosome and is involved in the biogenesis and function of this organelle. Little is known about the biosynthesis and metabolism of this lipid. We examined its FA composition and the incorporation of exogenous FA into LBPA in the human monocytic leukemia cell line...

L’objectif de ce travail a été de caractériser le Bis(Monoacylglycérol)Phosphate (BMP) dans les macrophages issus de la lignée monocytaire humaine THP-1, ainsi que d’appréhender son rôle possible dans la régulation de l’homéostasie du cholestérol. Nous avons montré par immunofluorescence que le BMP est localisé de façon quasi-exclusive au niveau de...