Neiva Tinti Oliveira

Federal University of Pernambuco | UFPE · Departamento de Micologia

ABSTRACT The knowledge of the geographical distribution of fungi is essential to promote the understanding of global and national fungal diversity patterns. In this study, we provide a literature-based checklist of Aspergillus, Penicillium, and Talaromyces species recorded in the biomes of the Brazilian Atlantic Forest and the Brazilian Pampa. Only...

This study aimed to evaluate the potential antagonistic role of Trichoderma in the control of Fusarium isolates belonging to the Fusarium oxysporum species complex (FOSC) and in the promotion of growth in Vigna unguiculata plants. The antagonistic action of Trichoderma isolates against Fusarium in vitro and in vivo was verified. Seven Trichoderma i...

The aim of the present study was to optimize the production of cellulolytic enzymes by C. gloeosporioides URM 7080 isolated from leaf lesions in Manihot esculenta showing symptoms of anthracnose. Cassava bran (MA) and crushed forage cactus (PA) were used as substrates for submerged fermentation at the concentrations of 0.5%, 0.75% and 1%; initial p...

Two new species were isolated during a survey on the mycobiota of soil of sugarcane fields in a deforested area of Atlantic Forest in Pernambuco, northeastern Brazil. Using a polyphasic approach, combining ITS, partial β-tubulin, calmodulin, and RPB2 gene sequences and morphological features, we described the new species Penicillium barbosae sp. no...

Fusarium incarnatum-equiseti species complex (FIESC) is considered as one of the richest insecticolous species. Fusarium species synthesize toxic secondary metabolites that are not fully understood. Mycotoxin production and pathogenicity on germinating seeds, seedlings, and leaves must be carefully studied for the use of Fusarium species in the bio...

A literature-based checklist of species of Aspergillus, Penicillium, and Talaromyces recorded in the Brazilian tropical dry forest (Caatinga), the largest tropical dry forest region in South America, is provided. A total of 130 species (60 Aspergillus, 57 Penicillium, and 13 Talaromyces) are reported. Soil was the most common substrate, with 122 sp...

The genus Fusarium is noted for including important plant pathogens and mycotoxin producers. Furthermore, many Fusarium lineages have been reported to be efficient in controlling insects and to exhibit promising characteristics for agricultural pest control such as causing high mortality rates and having fast action and abundant sporulation. In thi...

This article is the tenth series of the Fungal Diversity Notes, where 114 taxa distributed in three phyla, ten classes, 30 orders and 53 families are described and illustrated. Taxa described in the present study include one new family (viz. Pseudoberkleasmiaceae in Dothideomycetes), five new genera (Caatingomyces, Cryptoschizotrema, Neoacladium, P...

RESUMO Austropuccinia psidii é o agente etiológico de uma das principais doenças do jambeiro-do-pará: a ferrugem-amarela. Em uma ocorrência dessa doença foi constatada a ação de um fungo hiperparasito sobre uredossoro de A. psidii. A partir de folhas lesionadas, dois isolados de Fusarium foram caracterizados por análise morfológica e molecular. Os...

Based on morphological and molecular phylogenetic markers and the fertility of sexual crosses, two novel species of Fusarium associated with Dactylopius opuntiae (Hemiptera: Dactylopiidae) and Aleurocanthus woglumi (Hemiptera: Aleyrodidae) from northeastern Brazil are described as Fusarium caatingaense and F. pernambucanum. Partial sequences of fiv...

Resumo: Este estudo teve por objetivo avaliar o potencial de biocontrole in vitro de espécies de Trichoderma sobre Meloidogyne enterolobii, por meio de testes de parasitismo e inibição da eclosão de formas juvenis deste nematoide. Foram utilizados nove isolados de Trichoderma, pertencentes às espécies T. longibrachiatum (T1), T. asperellum (T2), T....

Penicillium and Talaromyces species have a worldwide distribution and are isolated from various materials and hosts, including insects and their substrates. The aim of this study was to characterize the Penicillium and Talaromyces species obtained during a survey of honey, pollen and the inside of nests of Melipona scutellaris. A total of 100 isola...

Penicillium and Talaromyces are fungal genera with high ecological and biotechnological importance. However, studies on exploration and ecology of these fungi in soils are scarce. The objectives of this study were to evaluate the species diversity of these genera in soils of sugarcane and fallow. Identification of the isolates was performed by morp...

Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this...

Cultivation of the prickly pear cactus, Opuntia ficus-indica, an important cattle food in the semiarid region of Brazil, has been increasingly compromised by predation by the cochineal scale Dactylopius opuntiae. Entomopathogenic fungi represent a low environmental impact alternative to controlling this insect pest, and the genus Fusarium has been...

The genus Monascus was described by van Tieghem (1884) to accommodate M. ruber and M. mucoroides, two species with non-ostiolate ascomata. Species delimitation in the genus is still mainly based on phenotypic characters, and taxonomic studies that include sequence data are limited. The genus is of economic importance. Species are used in fermented...

Material collected from commercial crops of cassava (Manihot esculenta Crantz) in 11 municipalities in the state of Paraíba included 27 isolates of the fungi Colletotrichum gloeosporoides f. sp. manihotis Henn (Penn.) and Fusarium oxysporum Schlent., which are pathogenic to the culture. From these isolates, cellulolytic enzyme complex production wa...

Isolates of Fusarium obtained from Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) demonstrated potential as biological control agents against that same insect, which is a pest on Opuntia ficus-indica L Miller. The isolates belong to two species complexes: Fusarium incarnatum-equiseti (FIESC - five species) and Fusarium fujikuroi (FFSC...

Novel species of fungi described in the present study include the following from Australia: Vermiculariopsiella eucalypti, Mulderomyces natalis (incl. Mulderomyces gen. nov.), Fusicladium paraamoenum, Neotrimmatostroma paraexcentricum, and Pseudophloeospora eucalyptorum on leaves of Eucalyptus spp., Anungitea grevilleae (on leaves of Grevillea sp.)...

Novel species of fungi described in the present study include the following from Australia: Vermiculariopsiella eucalypti, Mulderomyces natalis (incl. Mulderomyces gen. nov.), Fusicladium paraamoenum, Neotrimmatostroma paraexcentricum, and Pseudophloeospora eucalyptorum on leaves of Eucalyptus spp., Anungitea grevilleae (on leaves of Grevillea sp.)...

Soil is a complex biological system that plays a key role for plants and animals, especially in dry forests such as the Caatinga. Fungi from soils, such as Aspergillus and Penicillium, can be used as bioindicators for biodiversity conservation. The aim of this study was to isolate and identify species of Aspergillus and Penicillium in soil, from th...

Fusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels...

White piedra is a clinical condition caused by Trichosporon species (1,2). The most distinctive feature of this mycosis is the production of white-brown nodules adhered to the hair shaft that may easily be detached, in contrast to black piedra nodules (3). Despite awareness of white piedra in the medical field since the 19th century (4), there is l...

The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is...

The cassava is used in human and animal food, as well as in the industry. In the state of Paraíba, it is commercially explored on the Coast, in the microregion of "Brejo Paraibano" and as subsistence crop in inner regions of the state. In view of the lack of epidemiological studies of this crop in Paraiba State, the aim of this study was to evaluat...

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Microbial control of insects is based on the rational use of pathogens to maintain environmentally balanced pest population levels, and Metarhizium anisopliae has been the most studied and most utilized fungal species for that purpose. The natural genetic variability of entomopathogenic fungi is considered one of the principal advantages of microbi...

To survey and quantify the incidence of the diseases affecting cassava (Manihot esculenta) in commercial crops of the state of Paraiba - Brazil, samplings were conducted in 21 municipalities at four microregions of the state during the first semester of 2011. The detected diseases were brown leaf spot (Cercosporidium henningsii), white leaf spot (P...

Fusarium verticillioides is a pathogen of agriculturally important crops, especially maize. It is considered one of the most important pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and animals. Moreover, it is recognized as a cause of localized infections in immuno...

Neonatal candidemia can occur, however, infections caused by Candida pelliculosa are rare. Here, we describe an outbreak of candidemia caused by C. pelliculosa among babies hospitalized in a neonatal intensive care unit.

Anthracnose, caused by the fungus Colletotrichum gloeosporioides, is one of the most important diseases in cashew (Anacardium occidentale) cultivation. In this study, the genetic and pathogenic diversity of this microorganism isolated from cashew crops from Pernambuco State, Brazil, were evaluated by RAPD and ribosomal DNA-RFLP analysis. Based on t...

Non-albicans Candida species are being increasingly reported as causes of nosocomial fungal infections. For example, invasive candidiasis caused by C. tropicalis has been associated with hematologic malignancies. In this study, we report a fatal case of fungemia and a possible urinary and pulmonary infection in a leukemia patient that was due to a...

Transgenic Bt cotton expresses the Cry1Ac protein from Bacillus thuringiensis, which could influence the plant's capability to host endophytic fungi. The diversity of endophytic fungi in leaves, stems and roots from transgenic (Bt) and its isoline (non-Bt) cotton was evaluated during different plant developmental stages to investigate possible non-...

Expression analysis of the genes involved in germination, conidiogenisis and pathogenesis of Metarhizium anisopliae during its saprophytic and pathogenic life stages can help plan strategies to increase its efficacy as a biological control agent. We quantified relative expression levels of the nitrogen response regulator gene (nrr1) and a G-protein...

Trichosporon species are usually opportunistic pathogens. Here, we present a case of esophagitis caused by T. inkin in a 54-year-old woman with pulmonary cancer and severe neutropenia in whom the susceptibility profile of the isolate against azoles and polyenes was verified. The patient was diagnosed with esophagitis grade I of Wilcox, presenting s...

Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates a...

Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Gross...

Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers t...

Colletotrichum gloeosporioides is an important pathogen for a great number of economically important crops. During the necrotrophic phase of infection by Colletotrichum spp, the degradative enzymes of plant cell walls, such as pectate lyase, clearly increase. A gene pelB that expresses a pectate lyase was identified in isolates of C. gloeosporioide...

Four isolates of Sclerotinia sclerotiorum were tested for pathogenicity in IPA-10 variety bean plants (Phaseolus vulgaris L.), and all were pathogenic. Biological control in vitro was evaluated using eight isolates of Trichoderma spp. and, one of Ulocladium atrum. Chemical control in vitro with fungicides Thiophanate methyl, Iprodione and Carbendaz...

The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification is nowadays performed...

Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using...

Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel m...

Random Amplified Polymorphic DNA (RAPD) procedure was used to examine the genetic variability among fourteen isolates of Trichoderma and their ability to antagonize Rhizoctonia solani using a dual-culture assay for correlation among RAPD products and their hardness to R. solani. Seven oligodeoxynucleotide primers were selected for the RAPD assays w...

Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twen...

Under natural conditions a fungus, identified as Cladosporium uredinicola, was observed living as a parasite over rust lesions produced by Puccinia puta on leaves of Ipomoea fistulosa, a shrub commonly found in the State of Pernambuco, northeast of Brazil. Although recorded as a parasite of different rust species, C. uredinicola had never been ment...

Eight inoculation methods were studied to evaluate the pathogenicity among six isolates of Colletotrichum gloeosporioides, agent of anthracnose on passion fruits (Passiflora edulis). The isolates were selected by micelial growth and sporulation. The inoculations were made through suspension (1x10(6) conidia/ml) and micelial-agar discs (4mm in diame...

Conidia produced by a wild strain (E9) of the entomopathogenic fungus nd anisopliae var. anisopliae were exposed to gamma radiation in order to obtain new biotypes. At the 390 Gy dose there were obtained 48 colonies (MaE). On Complete Medium, 5 colonies (MaE 01, MaE 10, MaE 15, MaE 24, MaE 40) presented morphological changes in color while the colo...

The variation of total protein of spore during germination of strains E9, Pegro and BSA of Metarhizium anisopliae var. anisopliae was investigated. Minimal germination time (MGT, defined as the time during which 85% of the conidia germinated) for the three strains was 12 hours of incubation in minimal medium. When the total protein content of the t...